STAT1 Recombinant Monoclonal Antibody

Code: CSB-RA989713A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, IHC, IF, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

P42224

Target Names

STAT1

Alternative Names

Signal transducer and activator of transcription 1-alpha/beta (Transcription factor ISGF-3 components p91/p84), STAT1

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human STAT1

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6C10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA989713A0HU diluted at 1:50 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.40% DAB.
Immunofluorescence staining of Hela with CSB-RA989713A0HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 508-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing HepG2 cells stained with CSB-RA989713A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

STAT1 serves as a critical mediator of cellular responses to interferons and other cytokines, functioning as both a signal transducer and transcription factor. Upon activation, STAT1 translocates to the nucleus where it regulates genes involved in cell growth, apoptosis, and immune responses. Its dysregulation has been implicated in various malignancies and inflammatory conditions, making it a significant target for researchers investigating cancer biology, immune signaling, and epigenetic mechanisms.

This recombinant monoclonal antibody, generated from clone 6C10, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined genetic sequences rather than hybridoma supernatants, researchers can expect uniform performance across different lots, eliminating a common source of experimental variability that can complicate long-term studies or multi-site collaborations.

The antibody has been validated across multiple detection platforms, providing flexibility for diverse experimental approaches. Immunohistochemistry performed on paraffin-embedded human testis tissue demonstrates clear target detection using citrate buffer antigen retrieval on automated staining systems. Immunofluorescence studies in HeLa cells reveal the expected nuclear and cytoplasmic localization pattern characteristic of STAT1, while flow cytometry analysis in HepG2 cells confirms robust detection with clear separation from isotype control, supporting quantitative single-cell applications.

Raised against a synthetic peptide derived from human STAT1 and validated for human reactivity, this antibody is well-suited for researchers exploring interferon signaling pathways, JAK-STAT cascade dynamics, or STAT1's role in tumor biology. The unconjugated format allows pairing with secondary detection systems of choice, accommodating both standard and multiplexed experimental designs across ELISA, immunohistochemistry, immunofluorescence, and flow cytometry applications.

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Target Background

Function(From Uniprot)

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.

Gene References into Functions

we suggest that STAT1HDAC4 signaling induces malignant tumor features such as EMT and sphere formation in CUG2overexpressing cancer cells. PMID:30226605
Downregulated NDR1 protein kinase inhibits innate immune response by initiating an miR146a-STAT1 feedback loop. PMID:30018336
High STAT1 expression is associated with Melanoma. PMID:29150430
These results demonstrated that the IFNGinduced immunosuppressive properties of B7H1 in human BM and WJMSCs were mediated by STAT1 signaling, and not by PI3K/RACalpha serine/threonineprotein kinase signaling PMID:29901104
STAT1 plays a pivotal role as a tumor suppressor in glioma. PMID:29800921
the present study revealed a negative correlation between the expression of the STAT-1 gene and the glioma grade, as well as between STAT-1 and mutant p53 expression. The negative correlation between STAT-1 and the pathological level of glioma suggested that STAT-1 may be associated with the occurrence and development of glioma, and may be a diagnostic biomarker and therapeutic target for the malignancy of glioma. PMID:29620180
PARP9 and PARP14 regulate macrophage activation in macrophage cell lines treated with either IFNgamma or IL-4; PARP14 silencing induces pro-inflammatory genes and STAT1 phosphorylation in M(IFNgamma) cells, whereas it suppresses anti-inflammatory gene expression and STAT6 phosphorylation in M(IL-4) cells PMID:27796300
PVT1 interacts with STAT1 to inhibit IFN-alpha signaling and tumor cells proliferation. PMID:29715456
STAT1 is associated with giant cell tumor of bone recurrence, which might serve as biomarker for giant cell tumor of bone recurrence PMID:29651441
High STAT1 expression is associated with head and neck squamous cell carcinoma. PMID:29328389
The research described here specifies where in the JAK/STAT signaling cascade the IFN response is inhibited and which protein domain of nsP2 is responsible for IFN inhibition. The results illuminate new aspects of antiviral defense and CHIKV counterdefense strategies and will direct the search for novel antiviral compounds. PMID:29925658
These results reveal that STAT1 pS727 regulates growth and differentiation in JAK-STAT activated neoplasms and suggest that Mediator kinase inhibition represents a therapeutic strategy to regulate JAK-STAT signaling PMID:29239838
Transcription factor STAT1 regulates the expression of LINC00174 PMID:29729381
Dysregulation of the IFN-gamma-STAT1 signaling pathway in a cell line model of large granular lymphocyte leukemia PMID:29474442
STAT1b plays a key role in enhancing the tumor suppressor function of STAT1a, in ESCC, in a manner that can be amplified by IFN-gamma PMID:28981100
HSP90 is an upstream regulator of the ACK1-dependent phosphorylation of STAT1 and STAT3. PMID:28739485
These findings suggest that IFN-a can inhibit HCV replication through a STAT2-dependent but STAT1-independent pathway, whereas IFN-g induces ISG expression and inhibits HCV replication exclusively through a STAT1- and STAT2-dependent pathway. PMID:27929099
MxA inhibits hepatitis C virus replication through JAK-STAT pathway activation. PMID:29417241
These results indicate the potential involvement of STAT1 in the regulation of trophoblast behavior. Furthermore, STAT 1 functions are more efficiently inhibited by blocking its expression than its phosphorylation. PMID:28552376
Authors detected the ERK, p-ERK and STAT1 expression in 131 ESCC cases and 22 case-matched normal esophageal tissues adjacent to the tumors specimens. These findings provide pathological evidence that ERK/p-ERK is negatively correlated with STAT1 in ESCC. PMID:28431406
129:Stat1 (-/-) is a unique model for studying the critical origins and risk reduction strategies in age-related ER(+) breast cancer. In addition, it can be used in preclinical trials of hormonal and targeted therapies as well as immunotherapies. PMID:28865492
STAT1 knockdown using an inhibitor and siRNA attenuated the IL-17-mediated increases in IL-6, IL-8 and VEGF expression in A549 and H292 cells. PMID:27819281
Review of the role of STAT1 and STAT3 gain-of-function mutations in primary immunodeficiency/immunodysregulation disorders. PMID:28914637
IFN gamma induced upregulation of BCL6 was dependent on the classical STAT1 signaling pathway, and affected both major BCL6 variants. Interestingly, although IFN alpha induced stronger STAT1 phosphorylation than IFN gamma, it only slightly upregulated BCL6 in multiple myeloma lines. PMID:29510136
this study shows that miR-146a negatively regulates NK cell functions via STAT1 signaling PMID:26996068
Authors found that YY1 and STAT1 were upregulated in ox-LDL-stimulating macrophages followed by translocation in the nucleus and binding to the transcriptional promoter region of miR-29a, thus leading to the increase of miR-29a expression. PMID:28593745
Candidate biomarker genes such as CXCL10, IRF1, STAT1, IFIT2, and IFIT3 may be suitable therapeutic targets for ICM. PMID:28150292
Aberrant Th1 immune responses in biliary atresia promote the proliferation and secretion of hepatic stellate cells through the IFN-gamma/STAT1 pathway. PMID:28304404
Calcitriol treatment of the TL-1 cell line (model of T-LGLL) led to decreased phospho-Y701 STAT1 and phospho-Y705 STAT3 and increased vitamin D receptor (VDR) levels. PMID:27715403
These findings indicate that viral replication and inflammation are linked through a common IFNgamma-like, STAT-dependent pathway and that HIV-1-induced STAT1 and STAT3 signaling are involved in both inflammation and HIV-1 replication. PMID:28142265
Increased levels of STAT1 Protein in CD4 T-cells from systemic lupus erythematosus patients are associated with disease severity. PMID:28256939
miR203 acts as a tumor suppressor in glioblastoma by suppressing the pro-tumorigenic action of STAT1. PMID:27705947
Results show that high ph-STAT1 and ph-STAT3 tumor cell expression were associated with increased ER and PR, reduced tumor grade and necrosis. STAT1 and STAT3 expression appeared to be an important determinant of favorable outcome in patients with invasive ductal breast cancer suggesting that both act as tumor suppressor proteins in patients with ductal breast cancer. PMID:27769057
Results identified STAT1 as a central node of tumor-stimulated stromal signature and demonstrate that stromal STAT1 expression promotes tumor progression. PMID:28108623
The results of the present study indicate that ISTP may inhibit TARC/CCL17 production in human epidermal keratinocytes via the STAT1 signaling pathway and may be associated with the inhibition of IL33 production. PMID:28447741
In human gastrointestinal stromal tumors (GIST) cell lines, treatment with imatinib abrogated the IFNgamma-induced upregulation of PD-L1 via STAT1 inhibition. PD-1/PD-L1 blockade is a promising strategy to improve the effects of targeted therapy in GISTs PMID:27470968
STAT3/STAT1 ratios are better clinical predictors in colorectal carcinoma as compared to STAT3 or STAT1 levels alone. PMID:27191495
In a subgroup of Schizophrenic patients, blood levels of STAT1 were significantly higher compared to the control group. PMID:27820940
Suggest a positive feedback mechanism via the STAT1/3 pathway sustains cytokine production and reveal a reciprocal regulatory role of JAK/STAT in TNFalpha-mediated senescence. PMID:29176033
our findings suggested that OSM suppresses SLUG expression and tumor metastasis of lung adenocarcinoma cells through inducing the inhibitory effect of the STAT1-dependent pathway and suppressing the activating effect of STAT3-dependent signaling PMID:27486982
miR-2909 could play a vital role in prostate carcinogenesis through modulation of ISGylation system and TGFbeta signalling via STAT1/SOCS3. PMID:28622443
The phosphorylation of STAT1 promotes its binding to TRADD, and thus recruits Fas-associated protein with DD (FADD) and caspase 8 to form DISC complexes. PMID:28186502
The results demonstrate that cystatin B interferes with the STAT-1 signaling and IFN-beta-antiviral responses perpetuating HIV in macrophage reservoirs. PMID:27137788
propose that one molecule of C protein associates with the STAT1:STAT2 heterodimer, inducing a conformational change to an antiparallel form, which is easily dephosphorylated PMID:28978648
Transfections of undifferentiated shed cells with miR-450a-5p or miR-28-5p mimics or with miR-450a-5p or miR-28-5p antagonists demonstrated that these miRNAs might play a role as posttranscriptional controllers of STAT1 mRNA during osteoblastic differentiation. PMID:28407302
Data indicate that dysregulated IFN-gamma secretion by NK cells contributed to a significant defect in STAT1 in patients with advanced melanoma in response to IL-2 stimulation. PMID:27153543
our results first identified that the proper increase of PD-1/STAT1 may contribute to hematopoietic improvement and prolonged survival in lower risk MDS. Our study proposed that the PD-1-related strategy to treat MDS should be different for lower risk patients than it is for those with highly progressive characteristics. PMID:27686004
findings shed new light on the STAT1/miR-181a/PTEN pathway in colorectal cancer and add new insight regarding the carcinogenesis of colorectal cancer. PMID:28322462
In lipotoxic hepatocytes, MLK3 activates a MAPK signaling cascade, resulting in the activating phosphorylation of STAT1, and CXCL10 transcriptional upregulation. PMID:28262979
Decreased phosphorylated STAT1 expression was accompanied by increased replication of hepatitis C virus and hepatitis E virus. PMID:28442624