SRSF1 Recombinant Monoclonal Antibody

Code: CSB-RA909596A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human, Rat

Application: ELISA, WB, IHC, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

Q07955

Target Names

SRSF1

Alternative Names

Serine/arginine-rich splicing factor 1 (Alternative-splicing factor 1) (ASF-1) (Splicing factor, arginine/serine-rich 1) (pre-mRNA-splicing factor SF2, P33 subunit), SRSF1, ASF SF2 SF2P33 SFRS1

Species Reactivity

Human, Rat

Immunogen

A synthesized peptide from human SRSF1 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

12H9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate(30µg), Rat brain tissue lysate(30µg)
All lanes: SRSF1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 28 kDa
Observed band size: 33 kDa
Exposure time：20s
IHC image of CSB-RA909596A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA909596A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA909596A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

SRSF1, also known as ASF/SF2, is a pivotal serine/arginine-rich splicing factor that orchestrates constitutive and alternative pre-mRNA splicing events throughout the cell. Beyond its canonical role in splice site selection, SRSF1 influences mRNA export, stability, and translation, positioning it as a central regulator of gene expression. Dysregulation of SRSF1 has been implicated in oncogenic transformation and tumor progression, making it a compelling target for cancer biology and RNA processing research.

This recombinant monoclonal antibody, generated in rabbit against a synthetic peptide derived from human SRSF1, offers the reproducibility and sequence-defined consistency that demanding experimental workflows require. As a recombinant clone, it eliminates the lot-to-lot variability inherent to traditional hybridoma-derived antibodies, ensuring your results remain comparable across extended studies and collaborative projects.

Validation across multiple platforms demonstrates this antibody's experimental versatility. Western blot analysis detects SRSF1 in human SH-SY5Y neuroblastoma cells and rat brain tissue lysates, confirming cross-species reactivity between human and rat samples. The observed band at approximately 33 kDa runs slightly higher than the predicted 28 kDa molecular weight, a shift commonly attributed to post-translational modifications such as phosphorylation of the serine/arginine-rich domain. Immunohistochemistry staining has been validated in paraffin-embedded human colorectal cancer and small intestine tissues using citrate buffer antigen retrieval, while flow cytometry analysis confirms intracellular detection in permeabilized SH-SY5Y cells with clear signal separation from isotype controls.

Whether investigating splicing regulation in neurological disease models, characterizing SRSF1 expression patterns in tumor specimens, or examining RNA processing dynamics at the single-cell level, this antibody provides a reliable tool for advancing your research.

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Target Background

Function(From Uniprot)

Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.

Gene References into Functions

SRSF1 promotes vascular smooth muscle cell (VSMC) proliferation and injury-induced neointima formation. SRSF1 favors the induction of a truncated p53 isoform, Delta133p53, which has an equal proliferative effect and in turn transcriptionally activates Kruppel-like factor 5 (KLF5) via the Delta133p53-EGR1 complex, resulting in an accelerated cell-cycle progression and increased VSMC proliferation. PMID:28799539
repeat RNA-sequestration of SRSF1 triggers the NXF1-dependent nuclear export of C9ORF72 transcripts retaining expanded hexanucleotide repeats PMID:28677678
A -44 G to A "hot zone" putative functional noncoding variant of SRSF1 was found in an AML patient. It alters the binding activities of E2F6, ELF1, and ELK4, ELK4. PMID:29764005
We now show that the ability of SRPK1 to mobilize SRSF1 from speckles to the nucleoplasm is dependent on active CLK1. Diffusion from speckles is promoted by the formation of an SRPK1-CLK1 complex that facilitates dissociation of SRSF1 from CLK1 and enhances the phosphorylation of several serine-proline dipeptides in this SR protein PMID:29335301
Authors showed that Mir505-3p was capable of inhibiting tumor proliferation driven by SRSF1 in two neural tumor cell lines, Neuro-2a (N2a) and U251, exclusively in serum-reduced condition. Authors observed that the protein level of SRSF1 was gradually promoted by increasing concentration of serum. PMID:29120871
The present study suggested that the tumor suppressor miR30c may be involved in prostate cancer tumorigenesis, possibly via targeting ASF/SF2. PMID:28677791
It has been proposed that SF2/ASF has a protective role against JC virus reactivation in multiple sclerosis patients. PMID:27812788
Immune suppression of JC virus gene expression is mediated by SRSF1. PMID:26951564
ASF/SF2 is identified as a splicing regulator of cyclin T1, which contributes to the control of the subsequent transcription events. PMID:28422315
Findings suggest MALAT1 increases AKAP-9 expression by promoting SRPK1-catalyzed SRSF1 phosphorylation in CRC cells. These results reveal a novel molecular mechanism by which MALAT1 regulates AKAP-9 expression in CRC cells. PMID:26887056
high level of SF2, as a novel oncoprotein in RCC, was significantly associated with poor survival in a large cohort of RCC specimens. Taken together, our study presents a road map for the prediction and validation of miR-766-3p/SF2 axis and thus imparts a therapeutic way for further RCC progression. PMID:28657135
We present a joint atomistic molecular dynamics (MD) and experimental study of two RRM-containing proteins bound with their single-stranded target RNAs, namely the Fox-1 and SRSF1 complexes.The simulations predict unanticipated specific participation of Arg142 at the protein-RNA interface of the SRFS1 complex, which is subsequently confirmed by NMR and ITC measurements PMID:27193998
Using NMR spectroscopy with two separately expressed domains of SRSF1, we showed that several residues in the RNA-binding motif 2 interact with the N-terminal region of the RS domain (RS1). PMID:27091468
Especially, in SRSF1 morphants, bone cartilage formation was reduced in the brain and Nkx-2.5 expression was dramatically reduced in the heart of SRSF1 morphants. In addition, a dramatic reduction in functional chordin RNA in SRSF1 morphants was observed suggesting that chordin is one of the targets of SRSF1. Thus, we concluded that SRSF1 is an essential factor for pattern formation including heart, cartilage and germ lay PMID:27396620
results strongly support SRSF1 as a prognostic biomarker in SCLC and provide a rationale for personalized therapy in SCLC PMID:27093186
NSrp70 acts as a new molecular counterpart for alternative splicing of target RNA, counteracting SRSF1 and SRSF2 splicing activity. PMID:26797131
we have identified for the first time a potential mechanism that involves the essential splicing factor ASF/SF2 through which morphine regulates splicing specificity of the MOR encoding gene, OPRM1. PMID:26553431
Global splicing analysis with RNA-seq revealed that exons carrying the hnRNP H-binding GGGGG motif are predisposed to be skipped compared to those carrying the SRSF1-binding GGAGG motif in both human and mouse brains. PMID:26282582
We present experimental evidence that splicing factor SRSF1, SRSF2, U2AF35, U2AF65 and KHSRP expression levels in gastrointestinal tract (colon, gastric and pancreatic) tumors differ compare to healthy tissues and in cell lines PMID:26406946
These findings have revealed a molecular interplay between cellular SRSF1 and viral T-antigen in controlling JC virus gene expression. PMID:26596376
These include alternative splicing of SRSF1 itself. PMID:26273603
SF2 contributes to the elevated levels of ERK activation in hepatocellular carcinoma cells through modulating key component(s) downstream of growth factor receptors and upstream of ERK. PMID:26018840
These results suggest that MALAT1 may function as a promoter of gastric cancer cell proliferation partly by regulating SF2/ASF. PMID:24857172
SRSF1 regulates CD3zeta expression in human T cells and may contribute to the T cell defect in systemic lupus erythematosus. PMID:26134847
serine/arginine-rich splicing factor 1 (SRSF1) could specifically interact with RIG-I to facilitate RIG-I mediated production of type-I IFN that is triggered by cytosolic DNA. PMID:25658361
Using RNA-sequencing and 3-D cell cultures that mimic breast cancer, the authors identified SRSF1-regulated alternative splicing targets that are candidates for mediating SRSF1's oncogenic functions in mammary cells. PMID:26431027
The accumulated knowledge about SRSF1 provides critical insight into the integral role it plays in maintaining cellular homeostasis and suggests new targets for anticancer therapy. PMID:24807918
Data suggest that proline phosphorylation by CLK1/CDC-like kinase 1 (but not by SRPK1/serine/arginine-rich splicing factor kinase 1) regulates conformation and alternative splicing function of SFRS1 (serine/arginine-rich splicing factor 1). PMID:25529026
SRSF1 is a critical regulator that controls both RNA splicing and stability in the nucleus and functions in a Malat1-dependent manner. PMID:24468535
upon T cell activation, SRSF1 becomes limiting, and its function in CD6 exon 5 splicing is countered by an increase in CD6 transcription, dependent on chromatin acetylation. PMID:24890719
Data indicate that armadillo repeat protein ARVCF interacts with the splicing factors the splicing factor SRSF1 (SF2/ASF), the RNA helicase p68 (DDX5), and the heterogeneous nuclear ribonucleoprotein hnRNP H2. PMID:24644279
In the broader context of cancer pathology, the results suggest that SRSF1 might play a central role not only in the tumor cells, but also in the surrounding stroma. PMID:23966470
results demonstrate a novel mechanism of regulation of the splicing factor SRSF1 in human T cells and a potential molecular mechanism that controls its expression in SLE. PMID:24368769
Identification of new splicing events regulated by the oncogenic splicing factor SRSF1 in lung cancer using novel analytical tool. PMID:24371231
Specific induction of AS2 due to Alternative splicing is associated with epithelial ovarian cancer. PMID:23748175
Overexpression of SRSF1 and SRSF9 promote beta-catenin accumulation via the recruitment of beta-catenin mRNA and by enhancing its translation in an mTOR-dependent manner. PMID:23592547
The data establish a new view of SRSF1 protein regulation in which SRPK1 and CLK1 partition activities based on Ser-Pro versus Arg-Ser placement rather than on N- and C-terminal preferences along the RS domain. PMID:23707382
our results indicate that ASF/SF2 binds to a purine-rich region distant from both the previously published initiation site of HDV mRNA transcription and binding site of RNAP II, and suggest that this protein is not involved in HDV replication PMID:23349975
a new mechanism of posttranscriptional regulation of DIO1 and show deregulation of DIO1 expression in pituitary adenoma, possibly resulting from disturbed expression of SF2/ASF. PMID:23462647
High expression of ASF is associated with chronic myeloid leukemia. PMID:23228155
Results indicate that SF2/ASF regulates IL-2 production and that decreased SF2/ASF expression in systemic lupus erythematosus (SLE) T cells contributes to deficient IL-2 production. PMID:23319613
Results indicate that SRSF1 serves as an anti-apoptotic factor and potentially contributes to leukemogenesis in pediatric ALL patients by cooperating with PRMT1. PMID:22839530
Data indicate that depletion of SRSF1 in human cells compromises the association of splicing factors to nuclear speckles and influences the levels and activity of other SR proteins. PMID:22855529
SRSF1 is a direct target of the transcription factor oncoprotein MYC. PMID:22545246
Overexpression of SRSF1 rescues apoptosis of MCF-7 cells induced by Pnn depletion. PMID:22454513
In three-dimensional (3D) culture, SRSF1-overexpressing MCF-10A cells formed larger acini than control cells, reflecting increased proliferation and delayed apoptosis during acinar morphogenesis. PMID:22245967
ASF/SF2 translocates to the cytosol and regulates the alternative splicing of endoglin during senescence of endothelial cells. PMID:21668763
a novel intronic splicing enhancer that regulates caspase 9 RNA splicing and specifically interacts with SRSF1 was identified. PMID:21622622
negative regulation of the JC virus promoter by SF2/ASF may control reactivation of JCV replication in brain PMID:21297941
phosphorylation of the RS domain in SRSF1 induces a key molecular switch from intra- to intermolecular interactions, suggesting a plausible mechanism for the documented requirement for the phosphorylation/dephosphorylation cycle during pre-mRNA splicing. PMID:21536904