SPN Recombinant Monoclonal Antibody

Code: CSB-RA022590MA1HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, IHC

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Target Background

Product Details

Uniprot NO.

P16150

Target Names

SPN

Alternative Names

CD 43 antibody; CD43 antibody; CD43 antigen antibody; Galactoglycoprotein antibody; GALGP antibody; GPL 115 antibody; GPL115 antibody; Human gene for sialophorin antibody; Leucocyte sialoglycoprotein antibody; LEUK_HUMAN antibody; Leukocyte large sialoglycoprotein antibody; Leukocyte sialoglycoprotein antibody; Leukosialin antibody; LSN antibody; Ly-48 antibody; sialophorin (gpL115, leukosialin, CD43) antibody; Sialophorin antibody; Spn antibody

Species Reactivity

Human

Immunogen

Recombinant Human SPN protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

8E3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA022590MA1HU diluted at 1:50 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA022590MA1HU diluted at 1:50 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.

Description

SPN, also known as CD43 or leukosialin, is a heavily glycosylated transmembrane sialoglycoprotein expressed predominantly on hematopoietic cells, where it plays essential roles in T cell activation, leukocyte adhesion, and immune cell signaling. As one of the major surface glycoproteins on leukocytes, SPN serves as a valuable marker for studying immune cell populations, lymphocyte biology, and various hematological malignancies, making it a frequent target in both basic immunology research and clinical pathology applications.

This recombinant monoclonal antibody, clone 8E3, offers the reproducibility and consistency that demanding research protocols require. Because recombinant antibodies are produced from defined genetic sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures experimental results remain comparable across extended projects. The human IgG1 isotype format and affinity-chromatography purification further contribute to reliable performance with minimal background interference.

Validation studies demonstrate robust performance in immunohistochemistry applications on human tissues. Testing in paraffin-embedded human lung and lymph node sections using citrate buffer antigen retrieval at pH 6.0 shows clear specific staining at dilutions ranging from 1:50 to 1:200, giving researchers flexibility to optimize signal intensity for their particular tissue types and detection systems. The antibody has also been validated for ELISA applications, expanding its utility across different experimental workflows.

For investigators studying leukocyte biology, immune cell trafficking, or characterizing lymphoid tissues in health and disease states, this SPN antibody provides a dependable tool backed by documented performance in human tissue applications. Its suitability for immunohistochemistry makes it particularly valuable for researchers examining immune cell infiltration patterns or conducting tissue-based biomarker studies.

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Target Background

Function(From Uniprot)

Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells. Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR. Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1.; Protects cells from apoptotic signals, promoting cell survival.

Gene References into Functions

CD43 expression was 95.7% in atypical chronic lymphocytic leukemia cases. PMID:28713070
most non-hematopoietic neoplasms are negative for CD43 expression PMID:28807337
this study identifies a new signaling pathway for CD43 through the regulation of alternative functions of pyruvate kinase isoform M2, favoring cell survival following activation PMID:27606486
Forty percent of adenoid cystic carcinomas showed staining for CD43, while no cases of basal cell carcinoma were positive PMID:25551301
CD43 expression is a novel adverse prognostic factor for patients with diffuse large B-cell lymphomas PMID:25682152
CD43 polymorphisms are associated with TB susceptibility. PMID:25078322
Host membrane protein PSGL-1, CD43, and CD44 association with assembling HIV-1 Gag is driven by polybasic sequences present in the cytoplasmic tails of the membrane proteins and in Gag. PMID:25320329
When used as vaccine in mice, the 2/165 phagotope raised antibodies against the UN1/CD43 antigen, indicating that the 2/165 phagotope mimicked the UN1 antigen structure, and could represent a novel immunogen for cancer immunotherapy PMID:24356816
CD43 promotes cells transformation by preventing merlin-mediated contact inhibition of growth. PMID:24260485
We conclude that CD43 is an adverse prognostic marker in DLBCL, and is preferentially expressed in the non-GCB subgroup. PMID:23617469
CD43 regulates the threshold for T cell activation by targeting Cbl functions. PMID:21905200
Taken together, these results show that elevated calcium levels induce CD43 capping, and macrophages remove the cells if their nucleolin receptors can bind to the poly-N-acetyllactosaminyl chains of capped CD43. PMID:23400223
Targeting CD43 in A549 lung cancer cells, increased homotypic adhesion, decreased heterotypic adhesion and transendothelial migration, increased susceptibility to apoptosis and increased vulnerability to lysis by NK cells PMID:23015282
results suggest that, despite the high CD43 expression in both tumorous and nontumorous Langerhans cells (LCs), the JL1 epitope of CD43 is exposed in immature and neoplastic LCs. PMID:22790855
Compared to healthy controls, both CD43 mRNA and protein expressions were reduced in T cells from patients with SLE, and were inversely correlated with IgG. PMID:22613599
CD43 localizes to the nucleus, where it binds chromatin, co-localizes and co-immunoprecipitates with beta-catenin, and enhances the reporter gene expression regulated by beta-catenin PMID:22576689
The capping of CD43 on the cell surface is a strong signal for phagocytosis that allows phagocytes to differentiate between healthy and apoptotic cells without any additional membrane changes PMID:22466560
The anti-adhesive function of CD43 in colon carcinoma cells plays a role in the tumorigenesis and metastasis of colorectal carcinoma cells. PMID:22075155
there exists a negative feedback loop between p53 and CD43: CD43-dependent signaling activates p53, which in turn downregulates the expression of CD43 PMID:21947346
O-glycosylated CD43 and CD45 molecules on T cells regulates cell adhesion and favors the transmission of HTLV-1 from cell to cell. PMID:22171268
Data show that Pic, a class 2 SPATE protein produced by Shigella flexneri 2a targets a broad range of human leukocyte glycoproteins including CD43, CD44, CD45, CD93, CD162 and the surface-attached chemokine fractalkine. PMID:21768350
identification of the tumor antigen UN1 as the transmembrane CD43 sialoglycoprotein PMID:21372249
Expression of CD43 induces cell rounding, inhibition of cell re-attachment, augmentation of microvilli, and phosphorylation of Ezrin/Radixin/Moesin (ERM)in HEK293T cells. PMID:21045567
engagement of CD43 may, presumably through the repressing transcription, initiate a Bad-dependent apoptotic pathway. PMID:11773067
CD43 can be seen as a co-stimulatory cell surface constituent that can modulate HIV-1 expression in T lymphocytes PMID:12045189
down-regulation of CD43 mRNA levels occurs during activation of the cellline K562. This repression coincides with repression of the transcriptional activity of the CD43 gene promoter. PMID:12411317
Examination of expression of CD43 in different human cell lines and tumor cell lines PMID:12499775
Although ezrin-associated CD43 protein is excluded from the inhibitory, i.e., noncytolytic, NK cell immune synapse (IS), it is homogeneously distributed across the IS of activating conjugates. PMID:12626536
It was also observed that the mucins from colon carcinoma patients had MUC1-type mucins that carried both sialyl-Lewis a and x epitopes and CD43-type sialyl-Lewis a mucins with only low levels of sialyl-Lewis x epitopes. PMID:12820726
CD43 engagement on normal human T lymphocytes as well as in Jurkat cells results in transient phosphorylation of the zeta-chain and enhanced association of ZAP-70 and Vav to the zeta-chain. PMID:12902492
Overexpression of SPN causes activation of the tumor suppressor proteins p53 and ARF 1. PMID:14676827
Four phage antibodies were isolated and used in a preliminary immunohistochemistry study of CD43 expression on frozen colorectal adenoma and carcinoma tissue. PMID:14719063
findings suggest that the CD43 molecules expressed on CD4+ memory T cells may be capable of enhancing the costimulatory signaling and hence providing accessory functions to TCR-mediated activation processes PMID:15187099
Microarray analysis of inflammatory genes shows 1 group of genes coregulated by both stimuli and 2 further groups of target genes affected solely by costimulation or primarily by CD43. PMID:15280197
CD43 is expressed by a variety of carcinoma cell lines, and plays a role in tumour cell-peritoneal adhesion probably via interactions with its putative ligand ICAM-1 PMID:15449712
Data suggest that PKCtheta plays a critical role in the co-stimulatory functions of CD43 in human T cells. PMID:15522211
CD43 is a T-cell E-selectin ligand distinct from PSGL-1 which expands the role of CD43 in the regulation of T-cell trafficking. PMID:16269612
CD43 induces a signaling cascade that prolongs the duration of T cell receptor signaling, thereby supporting the temporality with which certain molecules are engaged as a mechanism to fine tune T cell signal quality, and ultimately immune function. PMID:16751378
early progenitors committed to hematopoietic development could be identified by surface expression of leukosialin PMID:16757688
Promotes cell growth and is a potential contributor to tumor development. PMID:17891181
Lymphoepithelial lesions pattern, CD43 coexpression, and clonal plasma cell component in extranodal marginal zone B-cell lymphoma (EMZL) is site-dependent, and the differences may aid in the diagnosis of EMZLs at different anatomic sites. PMID:17979485
triggering CD43 and the underlying signaling pathways enhance LFA-1 adhesiveness while CD43 also negatively regulates LFA-1 induction via other receptors by dynamic interaction with either LFA-1 or CD147. PMID:17996943
CD43 seems to be selectively expressed in a subset of adenoid cystic carcinomas and its significance in salivary tumors is discussed. PMID:18227725
The cleavage of neutrophil leukosialin (CD43) by cathepsin G releases its extracellular domain and triggers its intramembrane proteolysis by presenilin/gamma-secretase PMID:18586676
Streptococcus gordonii DL1 surface protein Hsa binds to the host cell membrane glycoproteins CD11b, CD43, and CD50 PMID:18678668

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Subcellular Location

Membrane; Single-pass type I membrane protein. Cell projection, microvillus. Cell projection, uropodium.; [CD43 cytoplasmic tail]: Nucleus. Nucleus, PML body.

Tissue Specificity

Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

Database Links

HGNC: 11249

UNIGENE: Hs.632188

KEGG: hsa:6693

STRING: 9606.ENSP00000353238

OMIM: 182160

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