SERPINA1 Recombinant Monoclonal Antibody

Code: CSB-RA977406A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human, Mouse

Application: ELISA, WB, IF, FC

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Target Background

Product Details

Uniprot NO.

P01009

Target Names

SERPINA1

Alternative Names

Alpha-1-antitrypsin (Alpha-1 protease inhibitor) (Alpha-1-antiproteinase) (Serpin A1) [Cleaved into: Short peptide from AAT (SPAAT)], SERPINA1, AAT PI

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human SERPINA1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

11G10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in:HEK293 whole cell lysate(30µg), Hela whole cell lysate(30µg), Jurkat whole cell lysate(30µg), Mouse liver tissue lysate(30µg)
All lanes:Antithrombin 3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Observed band size: 47 kDa
Exposure time：1min
Immunofluorescence staining of HepG2 cell with CSB-RA977406A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing HepG2 cells stained with CSB-RA977406A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

SERPINA1, commonly known as alpha-1-antitrypsin, serves as one of the most abundant serine protease inhibitors in human plasma, where it plays a critical protective role against neutrophil elastase-mediated tissue damage. Deficiency or dysfunction of this protein underlies alpha-1-antitrypsin deficiency, a hereditary condition associated with early-onset emphysema and liver disease, making SERPINA1 a significant target in pulmonary research, hepatology, and studies of protein misfolding disorders.

This recombinant rabbit monoclonal antibody, clone 11G10, offers the reproducibility and sequence-defined consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human SERPINA1, the recombinant format ensures that researchers can expect uniform performance across experiments and over time, eliminating the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations.

Validation across multiple applications demonstrates genuine experimental flexibility. Western blot analysis confirms robust detection in HEK293, HeLa, and Jurkat whole cell lysates, as well as mouse liver tissue, supporting cross-species reactivity between human and mouse samples. Immunofluorescence staining in HepG2 cells reveals clear cytoplasmic localization patterns when counterstained with DAPI, while flow cytometry analysis of permeabilized HepG2 cells shows distinct positive population shifts compared to isotype controls, confirming reliable intracellular detection.

The antibody arrives in a stabilized liquid format with glycerol for convenient long-term storage at ultra-low temperatures. Whether investigating inflammatory lung conditions, exploring hepatocyte biology, or characterizing SERPINA1 expression changes in disease models, this antibody provides a dependable tool for researchers working across proteostasis, inflammation, and metabolic liver disease research programs.

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Target Background

Function(From Uniprot)

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.; reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

Gene References into Functions

lower levels of serum alpha-1 antitrypsin were more frequent in nonidiopathic interstitial pneumonia patients than idiopathic interstitial pneumonia without an increase in genetic polymorphism; the difference was not statistically significant PMID:28425234
High A1AT expression is associated with HIV infection. PMID:29351445
Mutation GLU342LYS (rs28929474) and GLU264VAL (rsl7S80) in gene SERPINAl in patients with various stages of Pneumoconiosis correlates with early development and unfavorable course of Pneumoconiosis in post-contact period. PMID:30351692
Mutations in the alpha-1-antitrypsin and Wilson's genes may act as cofactors in the pathogenesis of fatty liver diseases. PMID:29324588
Data suggest alpha-1-antitrypsin (A1AT) and APOH (beta-2-glycoprotein-1) could represent promising markers for renal carcinogenesis in Von Hippel-Lindau disease (VHLD) patients. PMID:28004973
The two genetic associations with severe liver disease that had been suspected previously (one SNP for SERPINA1 and another for MAN1B1) were not confirmed in our cohort. Moreover, the haplotype analysis identified only one major genetic background for the SERPINA1 Z-allele, allowing us to exclude the presence of a frequent modifier SNP within. PMID:28887821
The reduction in circulating AAT levels associated to the PI*Zla palma allele was similar to that of PI*Z allele, representing a risk of impairment in lung function. PMID:28947017
Undifferentiated ASC may require priming to secrete functional A1AT, a potent antiprotease that may be relevant to stem cell therapeutic effects. PMID:28825379
The high expression of SERPINA1 indicates a poor prognosis of high-grade gliomas PMID:29309973
associations of PiMZ genotype with chronic obstructive pulmonary disease, lung function, and emphysema in whites and African Americans PMID:28380308
A1AT polymorphisms may be associated with human retrovirus infections in an ethnically mixed population from the Amazon region of Brazil. PMID:29030134
Results provide evidence that a non-synonymous single-nucleotide variant in SERPINA1 gene encoding alpha-1 antitrypsin as a major risk gene for large artery stroke. PMID:28265093
Compound heterozygous Alpha-1 antitrypsin Pi*SZ genotype is relatively frequent in Caucasian subjects resident in Europe, and in their descendants residing in the American continent, Australia, and New Zealand. PMID:28652721
The mean cerebrospinal fluid signals of the most acidic serpinA1 isoform differed significantly between Parkinson patients with dementia, without dementia and healthy controls. Patients above the cut-off of 6.4 have a more than six times higher risk for an association with dementia compared to patients below the cut off. PMID:27184740
This study suggested that high a1-antitrypsin (AAT)expression might be a negative prognostic marker for lung adenocarcinoma. AAT promoted lung adenocarcinoma metastasis, whose functional target may be fibronectin . Our findings provide new insight into the mechanisms of lung adenocarcinoma metastasis PMID:28440399
this study identified a carbamylated peptide of A1AT as an antigenic target of autoantibodies in patients with rheumatoid arthritis PMID:28291659
Novel RNA-binding activity of NQO1 promotes SERPINA1 mRNA translation. PMID:27515817
ZAAT ER clearance resulted from enhancing ERdj3-mediated ZAAT degradation by silencing ERdj3 while simultaneously enhancing autophagy. In this context, ERdj3 suppression may eliminate the toxic gain of function associated with polymerization of ZAAT PMID:28419579
In this study, the contribution of rare SERPINA1 alleles into AATD should not be neglected in the diagnosis practice given there is a wide spectrum of variants originated by mutation and sometimes shuffled between chromosomes by recombination. PMID:27296815
evaluation of serum proteins reveals that SERPINA1, SERPINA3 and SERPINC1 could be useful to discriminate healthy from colorectal carcinoma patients with a high sensitivity and specificity PMID:27492143
Alpha-1-antitrypsin for the treatment of steroid-refractory acute gastrointestinal graft-versus-host disease. PMID:28699171
this study proved that SYVN1 enhances SERPINA1(E342K)/ATZ degradation through SQSTM1-dependent autophagy and attenuates SERPINA1(E342K)/ATZ cytotoxicity. PMID:28121484
Recombinant human alpha-1 antitrypsin-Fc fusion protein reduces acute myocardial inflammatory injury after ischemia-reperfusion in the mouse leading to preservation of viable myocardium and systolic function. PMID:26945157
report association of APOE and TOMM40 with behavioural variant frontotemporal dementia, and ARHGAP35 and SERPINA1 with progressive non-fluent aphasia. PMID:28387812
Our results indicated a high prevalence of SERPINA1 alleles in the normal Saudi population and an association between alpha-1 antitrypsin deficiency and COPD development. PMID:29049242
findings highlight A1AT as a potential serum scavenger of hemin and suggest that the commercial preparations of human plasma A1AT might prove to be useful therapeutics in conditions associated with hemolysis. PMID:28716864
Reduced alpha-1-antitrypsin and elevated IL-23 csf levels were seen in amyotrophic lateral sclerosis patients. PMID:27245439
the results of this study demonstrate a previously unknown role for P2 residue in determining inhibitory specificity of A1AT. PMID:28922398
high A1AT expression correlated with increased tumor stage, elevated p53 staining and shorter overall survival in lung adenocarcinoma patients. PMID:28368395
These results clearly demonstrate that the association of malectin with ribophorin I is required to capture misfolded alpha1-antitrypsin and direct them to the degradation pathway. PMID:25451265
nNIF peptide is an inhibitor of NET formation in umbilical cord blood. PMID:27599294
AAT heterozygous status in rheumatoid arthritis is strongly associated with positive anti-citrullinated peptide autoantibodies and may define a distinct subset of patients with increased disease severity. PMID:28409899
Report alpha-1 antitrypsin Pi*Z gene frequency and Pi*ZZ genotype numbers worldwide. PMID:28243076
SNP rs8004738 of SERPINA1 gene significantly interacted with smoking status and was associated with a higher risk for COPD in the Chinese population. PMID:28138235
tissue specific expression and regulation of short transcripts of SERPINA1 gene and the presence of C-terminal peptides in supernatants from activated neutrophils PMID:28107454
SERPINA1 variants are associated with ANCA-associated vasculitis risk. PMID:28029757
Although the observed effect size of hAAT administration was limited and therefore the clinical relevance of our findings should be evaluated carefully, these data support the potential of this natural protein to ameliorate ischemic and inflammatory conditions. PMID:28235038
Report incorporation of the Mmalton allele-specific genotyping assay in the diagnostic algorithm of alpha-1-antitrypsin deficiency. PMID:27877030
The expression and intracellular accumulation of mutated Z-alpha-1 antitrypsin (the most common mutation) compromises macrophage function and contributes to emphysema development. Macrophages from individuals with Z-AAT have more proteolytic activity on ECM than normal. This abnormal activity is not abrogated by exogenous AAT and may be the result of cellular dysfunction induced by intracellular accumulation of Z-AAT. PMID:28362108
hAAT inhibits DC activation and function, and it also attenuates autoimmunity and renal damage in the MRL/lpr lupus model PMID:27232337
The deficiency PI*Z A1AT allele is considerably more common in patients with chronic respiratory disorders than in the general Polish population. PMID:26987331
Norursodeoxycholic acid administration is associated with increased autophagy, reduced A1AT protein accumulation, and reduced liver injury in a model of A1AT deficiency. PMID:27102560
Fecal enzyme-linked immunosorbent assay showed that alpha-1-antitrypsin level was able to distinguish cholangiocarcinoma patients from normal individuals. In conclusion, alpha-1-antitrypsin is a potential marker for early diagnosis of cholangiocarcinoma PMID:28618946
Reactive center loop insertion in alpha 1-Antitrypsin captured by accelerated molecular dynamics simulation has been presented. PMID:27995800
This aberrant conformation, induced by the loss of interactions from the Glu-342 side chain, explains why Z alpha1-AT is prone to polymerization and readily binds to a 6-mer peptide, and it supports that annealing of s5A into the central beta-sheet is a crucial step in the serpins' metastable conformational formation. The demonstration that the aberrant conformation can be rectified through stabilization of the labile ... PMID:27246852
The Alpha-1 antitrypsin (A1AT) deficiency (A1ATD) is a genetic autosomal recessive disease, characterized by low serum levels of A1AT. PMID:27855621
This work demonstrates a simple and efficient one-step purification of ralpha1-PI from transgenic plants, which is an essential prerequisite for further therapeutic development. PMID:26852026
Altered glycosylation, expression of serum haptoglobin and alpha-1-antitrypsin in chronic hepatitis C, hepatitis C induced liver cirrhosis and hepatocellular carcinoma patients. PMID:27034286
The PI-MZ rare (2%) SNP effect is nearly four times greater than the 'top' common height SNP in HMGA2. However, height only partially attenuates the SERPINA1-FEV1 or FVC association (around 50%) and vice versa PMID:26831755
We aimed to explore the features of Chronic Obstructive Pulmonary Disease in subjects carrying the PIMMmalton mutation, which is the most prevalent alpha-1 antitrypsin variant in Tunisia PMID:26071129

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Subcellular Location

Secreted. Endoplasmic reticulum. Note=The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum.; [Short peptide from AAT]: Secreted, extracellular space, extracellular matrix.

Protein Families

Serpin family

Tissue Specificity

Ubiquitous. Expressed in leukocytes and plasma.

Database Links

HGNC: 8941

UNIGENE: Hs.525557

KEGG: hsa:5265

STRING: 9606.ENSP00000348068

OMIM: 107400

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