RUNX3/RUNX2/RUNX1 Recombinant Monoclonal Antibody

Code: CSB-RA616024A0HU

Rate （N/A）
Review （0）

Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, FC

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

Q01196 Q13761 Q13950

Target Names

RUNX3/RUNX2/RUNX1

Alternative Names

Acute myeloid leukemia 2 protein antibody; Acute myeloid leukemia gene 2 antibody; AML 2 antibody; AML2 antibody; CBF alpha 3 antibody; CBF-alpha-3 antibody; CBFA 3 antibody; CBFA3 antibody; Core binding factor alpha 3 subunit antibody; core binding factor antibody; Core binding factor runt domain alpha subunit 3 antibody; Core binding factor subunit alpha 3 antibody; core-binding factor antibody; Core-binding factor subunit alpha-3 antibody; Oncogene AML 2 antibody; Oncogene AML-2 antibody; PEA2 alpha C antibody; PEA2-alpha C antibody; PEBP2 alpha C antibody; PEBP2-alpha C antibody; Pebp2a3 antibody; PEBP2aC antibody; Polyomavirus enhancer binding protein 2 alpha C subunit antibody; Polyomavirus enhancer-binding protein 2 alpha C subunit antibody; runt domain alpha subunit 3 antibody; runt related transcription factor 3 antibody; Runt-related transcription factor 3 antibody; RUNX 3 antibody; Runx3 antibody; RUNX3_HUMAN antibody; SL3 3 enhancer factor 1 alpha C subunit antibody; SL3-3 enhancer factor 1 alpha C subunit antibody; SL3/AKV core binding factor alpha C subunit antibody; SL3/AKV core-binding factor alpha C subunit antibody; Transcription factor AML2 antibodyAcute myeloid leukemia 1 antibody; Acute myeloid leukemia 1 protein antibody; alpha subunit core binding factor antibody; AML 1 antibody; AML1 antibody; AML1 EVI 1 antibody; AML1 EVI 1 fusion protein antibody; Aml1 oncogene antibody; AMLCR 1 antibody; AMLCR1 antibody; CBF alpha 2 antibody; CBF-alpha-2 antibody; CBFA 2 antibody; CBFA2 antibody; Core binding factor alpha 2 subunit antibody; Core binding factor runt domain alpha subunit 2 antibody; Core-binding factor subunit alpha-2 antibody; EVI 1 antibody; EVI1 antibody; HGNC antibody; Oncogene AML 1 antibody; Oncogene AML-1 antibody; OTTHUMP00000108696 antibody; OTTHUMP00000108697 antibody; OTTHUMP00000108699 antibody; OTTHUMP00000108700 antibody; OTTHUMP00000108702 antibody; PEA2 alpha B antibody; PEA2-alpha B antibody; PEBP2 alpha B antibody; PEBP2-alpha B antibody; PEBP2A2 antibody; PEBP2aB antibody; Polyomavirus enhancer binding protein 2 alpha B subunit antibody; Polyomavirus enhancer-binding protein 2 alpha B subunit antibody; Run1 antibody; Runt related transcription factor 1 antibody; Runt-related transcription factor 1 antibody; RUNX 1 antibody; Runx1 antibody; RUNX1_HUMAN antibody; SL3 3 enhancer factor 1 alpha B subunit antibody; SL3-3 enhancer factor 1 alpha B subunit antibody; SL3/AKV core binding factor alpha B subunit antibody; SL3/AKV core-binding factor alpha B subunit antibodyAcute myeloid leukemia 3 protein antibody; Alpha subunit 1 antibody; AML3 antibody; CBF alpha 1 antibody; CBF-alpha-1 antibody; CBFA1 antibody; CCD antibody; CCD1 antibody; Cleidocranial dysplasia 1 antibody; Core binding factor antibody; Core binding factor runt domain alpha subunit 1 antibody; Core binding factor subunit alpha 1 antibody; Core-binding factor subunit alpha-1 antibody; MGC120022 antibody; MGC120023 antibody; Oncogene AML 3 antibody; Oncogene AML-3 antibody; OSF 2 antibody; OSF-2 antibody; OSF2 antibody; Osteoblast specific transcription factor 2 antibody; Osteoblast-specific transcription factor 2 antibody; OTTHUMP00000016533 antibody; PEA2 alpha A antibody; PEA2-alpha A antibody; PEA2aA antibody; PEBP2 alpha A antibody; PEBP2-alpha A antibody; PEBP2A1 antibody; PEBP2A2 antibody; PEBP2aA antibody; PEBP2aA1 antibody; Polyomavirus enhancer binding protein 2 alpha A subunit antibody; Polyomavirus enhancer-binding protein 2 alpha A subunit antibody; Runt domain antibody; Runt related transcription factor 2 antibody; Runt-related transcription factor 2 antibody; RUNX2 antibody; RUNX2_HUMAN antibody; SL3 3 enhancer factor 1 alpha A subunit antibody; SL3-3 enhancer factor 1 alpha A subunit antibody; SL3/AKV core binding factor alpha A subunit antibody; SL3/AKV core-binding factor alpha A subunit antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human RUNX3/RUNX2/RUNX1

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1C3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Overlay Peak curve showing jurkat cells stained with CSB-RA616024A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

The RUNX family of transcription factors—RUNX1, RUNX2, and RUNX3—serves as master regulators across diverse biological processes, from hematopoiesis and skeletal development to immune cell differentiation and tumor suppression. These proteins share a highly conserved Runt domain that mediates DNA binding and heterodimerization with core-binding factor beta, making them central players in developmental biology and disease pathogenesis. RUNX1 is particularly notable for its frequent involvement in chromosomal translocations associated with acute myeloid leukemia, while RUNX2 drives osteoblast differentiation and RUNX3 functions as a tumor suppressor in gastric and other epithelial cancers.

This recombinant monoclonal antibody, clone 1C3, offers researchers studying RUNX family members the advantages inherent to recombinant technology. Generated in rabbit and produced from a defined sequence, this antibody delivers the lot-to-lot consistency essential for longitudinal studies and reproducible results across experiments. The affinity-chromatography purification ensures high specificity, while the unconjugated format provides flexibility for downstream detection strategies.

Validation through flow cytometry demonstrates effective detection in Jurkat cells, a human T-cell leukemia line, using intracellular staining protocols with fixation and permeabilization. The clear separation between antibody-stained and isotype control populations at 1:100 dilution confirms reliable signal discrimination, with recommended working dilutions ranging from 1:50 to 1:200 for flow cytometry applications. The antibody is also validated for ELISA, expanding its utility across experimental platforms.

This antibody supports investigations into hematopoietic malignancies, bone development disorders including cleidocranial dysplasia, and cancer biology where RUNX family dysregulation contributes to disease progression.