RPS6KA6 Recombinant Monoclonal Antibody

Code: CSB-RA264975A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, IHC, FC

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Target Background

Product Details

Uniprot NO.

Q9UK32

Target Names

RPS6KA6

Alternative Names

Ribosomal protein S6 kinase alpha-6 (S6K-alpha-6) (EC 2.7.11.1) (90 kDa ribosomal protein S6 kinase 6) (p90-RSK 6) (p90RSK6) (Ribosomal S6 kinase 4) (RSK-4) (pp90RSK4), RPS6KA6, RSK4

Species Reactivity

Human

Immunogen

A synthesized peptide from human RPS6KA6 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

17F3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA264975A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA264975A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

RPS6KA6, also known as RSK4, is a member of the p90 ribosomal S6 kinase family that plays important roles in cellular signaling pathways downstream of MAPK activation. This kinase participates in the regulation of cell growth, proliferation, and survival, making it a significant target for researchers investigating signal transduction mechanisms, developmental biology, and disease processes where RSK family members contribute to aberrant cellular behavior.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human RPS6KA6, offers the reproducibility and consistency that demanding experimental workflows require. As a sequence-defined recombinant clone, it eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit monoclonal format combines high specificity with robust signal generation, providing reliable detection of your target across experiments.

Validation studies demonstrate this antibody's utility across multiple applications. Immunohistochemistry performed on paraffin-embedded human testis tissue using a Leica Bond system with citrate buffer antigen retrieval shows specific staining at dilutions between 1:50 and 1:200. Flow cytometry analysis using SH-SY5Y neuroblastoma cells confirms intracellular detection capability, with clear separation between specific signal and isotype control populations when cells are fixed and permeabilized prior to staining.

The unconjugated format provides flexibility for researchers to pair this antibody with their preferred detection systems, whether using HRP-polymer systems for chromogenic IHC or fluorophore-conjugated secondary antibodies for flow cytometry applications. This versatility makes the antibody well-suited for studies exploring RSK4 expression patterns in human tissues and cell models relevant to cancer biology, neurological research, and developmental signaling investigations.

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Target Background

Function(From Uniprot)

Constitutively active serine/threonine-protein kinase that exhibits growth-factor-independent kinase activity and that may participate in p53/TP53-dependent cell growth arrest signaling and play an inhibitory role during embryogenesis.

Gene References into Functions

RSK4 constitutes a putative tumor suppressor gene for non-small cell lung carcinoma. PMID:30143490
The regulatory role of RSK4 in breast cancer development was mediated by AKT and extracellular signalregulated kinase (ERK) signaling pathways and the expression of RSK4 was altered by DNA methylation in promoter regions. PMID:28731146
RSK4 is expressed at low levels in malignant ovarian tumors, which correlates with advanced stages of the disease. PMID:26732474
downregulation of RSK4 expression is indicated to be associated with tumor cell proliferation and invasion, and silencing of the RSK4 may be involved in the development and progression of breast cancer PMID:26397146
Low RSK4 expression is correlated with advanced clinical pathologic classifications and is a poor overall survival in colorectal cancer patients PMID:25197367
RSK4 mRNA is significantly decreased in most of breast cancer tissues compared with paired non-cancerous tissues, due to promoter hypermethylation; frequent epigenetic inactivation might have a role in precancerous lesions or early cancer PMID:24338215
evaluated expression of RSK4 in renal cell carcinoma(RCC)tissues, analysed relationship between RSK4 expression and clinicopathological features of RCC patients and explored potential molecular mechanisms of RSK4 in RCC cell lines; expression pattern and molecular mechanisms of RSK4 in RCCs indicate it could be a potential independent prognostic factor PMID:23942078
Overexpression of RSK3 or RSK4 supports tumor cell proliferation upon PI3K inhibition both in vitro and in vivo therby contributing to drug resistance. PMID:23635776
findings reveals a major role of PRKX, TTBK2 and RSK4 in triggering Sunitinib resistance formation; data suggest transcriptional regulation of these kinases together with other proteins might play an important role in formation of Sunitinib resistance by affecting transcription factors PMID:22020623
Suggest that RSK4 may serve as a mediator of endothelial progenitor cell senescence in diabetes mellitus. PMID:22297070
The expression level of RSK-4 mRNA in breast cancer was significantly lower than those in normal breast tissues and breast benign lesions tissues. The down-regulation of RSK-4 expression in breast caner suggests that it is a breast cancer suppressor gene. PMID:21875487
Data indicate that RSK4 appears to be epigenetically silenced in endometrial cancer as evidenced by hypermethylation. PMID:21372219
importance of RSK4 for regulating senescence and indicate that downregulation of this kinase could be an important element in facilitating cell transformation. PMID:21239520
The unusual regulation and growth factor-independent activity of ribosomal S6 kinase 4 (RSK4) indicate that it is functionally distinct from other RSKs and may explain why RSK4 can participate in non-growth factor signaling. PMID:15632195
Downregulation of RPS6KA6 is associated with colon carcinoma. PMID:16865262
RT-PCR data show high expression of putative tumor suppressor genes Rsk4 and RbAp46 in 47% and 79% of breast carcinoma cases, respectively, whereas Cldn2 was down-regulated in 52% of breast cancer cases compared with normal adjacent tissues. PMID:17314274
RSK4 expression may limit the oncogenic, invasive, and metastatic potential of breast cancer cells. PMID:18628456
Results support the concept that RSK4 may be an important tumor suppressor gene by modulating senescence induction and contributing to cell proliferation control in colon carcinogenesis and renal cell carcinomas. PMID:19584160