Phospho-SMAD5 (S463+S465) Recombinant Monoclonal Antibody

Code: CSB-RA859108A463phHU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, IHC

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Target Background

Product Details

Uniprot NO.

Q99717

Target Names

SMAD5

Alternative Names

DKFZp781C1895 antibody; DKFZp781O1323 antibody; Dwfc antibody; hSmad5 antibody; JV5 1 antibody; JV5-1 antibody; MAD homolog 5 antibody; MAD, mothers against decapentaplegic homolog 5 antibody; MADH 5 antibody; MADH5 antibody; Mothers against decapentaplegic homolog 5 antibody; mothers against decapentaplegic, drosophila, homolog of, 5 antibody; Mothers against DPP homolog 5 antibody; MusMLP antibody; SMA and MAD related protein 5 antibody; SMAD 5 antibody; SMAD family member 5 antibody; SMAD, mothers against DPP homolog 5 antibody; Smad5 antibody; SMAD5_HUMAN antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human Phospho-SMAD5 (S463+S465)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2G11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA859108A463phHU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Description

SMAD5 serves as a critical intracellular mediator of bone morphogenetic protein signaling, translating extracellular BMP cues into transcriptional responses that govern cell fate decisions, differentiation, and tissue homeostasis. Phosphorylation at the C-terminal serine residues S463 and S465 represents the activating modification that enables SMAD5 to translocate to the nucleus and regulate target gene expression. Detecting this specific phosphorylation event allows researchers to monitor active BMP signaling in real time, making it essential for studies in developmental biology, stem cell research, and disease contexts where BMP pathway dysregulation plays a role.

This recombinant monoclonal antibody, generated in rabbit and designated clone 2G11, offers the reproducibility and consistency that phospho-specific detection demands. Because recombinant antibodies are produced from defined sequences rather than hybridoma-derived sources, researchers can expect uniform performance across experiments and over time, eliminating the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The antibody was raised against a synthetic phosphopeptide corresponding to the human Phospho-SMAD5 sequence surrounding S463 and S465, ensuring precise recognition of the activated form.

Validation in immunohistochemistry demonstrates reliable performance in paraffin-embedded human pancreatic tissue at dilutions ranging from 1:50 to 1:200, with optimized staining achieved using citrate buffer antigen retrieval at pH 6.0. This makes the antibody well-suited for researchers investigating BMP pathway activation in archival tissue samples or clinical specimens. Additional compatibility with ELISA extends its utility for quantitative applications.

For investigators exploring signal transduction mechanisms, particularly those examining how BMP signaling influences cellular responses in normal physiology and disease states, this antibody provides a dependable tool for tracking pathway activation at the level of SMAD5 phosphorylation.

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Target Background

Function(From Uniprot)

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

Gene References into Functions

We demonstrated high expression of miR-145 associated with late stage and unfavorable prognosis of esophageal cancer. We identified SMAD5 as direct target of miR-145, the suppressed expression of which consequently led to increased cell proliferation and migration/invasion. PMID:29852786
Smad5 acts as a intracellular pH messenger and maintains the bioenergetic homeostasis of cells by regulating cytoplasmic metabolic machinery. PMID:28675158
Data suggest that differences in expression levels in granulosa-like tumor cells and granulosa cells (GCs) from patients with polycystic ovary syndrome (PCOS) are due to increased expression of microRNA-27a-3p in GCs caused by insulin resistance in PCOS; microRNA-27a-3 expression is up-regulated in GCs in PCOS; overexpression of miR-27a-3p inhibits SMAD5 expression and promotes apoptosis. (SMAD5 = SMAD family member 5) PMID:29029022
Here the involvement of the pathway in adult brain function is suggested. This exploratory study establishes a strategy to better identify neuronal molecular signatures that are potentially associated with mental illness and cognitive deficits. We propose that the SMAD pathway may be a novel target in addressing cognitive deficit of SZ in future studies. PMID:27727244
miR-23a and miR-27a target SMAD5 and regulate apoptosis in human granulosa cells via the FasL-Fas pathway PMID:26400397
Our findings suggest that suppression of miR-222-3p activity promoted osteogenic differentiation hBMSCs through regulating Smad5-RUNX2 signaling axis. PMID:26809090
The polycomb group protein L3MBTL1 represses a SMAD5-mediated hematopoietic transcriptional program in human pluripotent stem cells. PMID:25754204
among the 15 SNPs, rs3206634 was significantly associated with KD in a recessive model (odds ratio = 2.31, p = 0.019), whereas there was no association between any of the 15 SNPs and CALs. PMID:24163009
Specific gene siRNAs knock-down further confirmed the osteogenic effects of Genistein on BMP2, SMAD5 and RUNX2 protein expression PMID:24339730
ATP production by NaF promotes hypertrophy-like changes via activation of phospho-Smad5 PMID:23384547
found that restoration of SMAD5, in addition to the TGF-beta type II receptor, which was epigenetically silenced by the latent viral protein latency-associated nuclear antigen, sensitized BC3 cells to the cytostatic effect of TGF-beta signaling PMID:22013049
Studies indicate SMAD5beta isoform plays role in hematopoietic stem cell homeostasis. PMID:20148926
regulatory signals are active at transcriptionally subnuclear sites PMID:12060751
Smad5 activation by BMP4 in human hematopoietic cells results in significantly increased proliferation of erythroid progenitors and formation of glycophorin-A+ cells. PMID:12064918
up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection PMID:12473652
Results demonstrate a mitochondrial distribution of Smad5 in non-stimulated chondroprogenitor cells. PMID:12849988
SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma PMID:14670176
In mature human B cells, BMP-6 inhibited cell growth, and rapidly induced phosphorylation of Smad5. PMID:15877825
We used homology-modeling techniques to generate a reliable molecular model of the Smad5 MH1 domain based on the crystal structure of Smad3 MH1 domain PMID:16243555
SMAD5 gene was found to be associated with schizophrenia. PMID:18298822