Phospho-Histone H1.4 (T17) Recombinant Monoclonal Antibody

CSB-RA010380A17phHU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, IHC, IF

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Target Background

Product Details

Uniprot NO.

P10412

Alternative Names

H1 histone family member 4 antibody; H1.4 antibody; H14_HUMAN antibody; H1E antibody; H1F4 antibody; Hist1h1e antibody; Histone 1 H1e antibody; Histone cluster 1 H1e antibody; Histone H1 antibody; Histone H1.4 antibody; Histone H1B antibody; MGC116819 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3E1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Scope Application

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:500
IF	1:30-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Protocols

ELISA Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol

Datasheet & COA

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Phospho-Histone H1.4 (T17) Recombinant Monoclonal Antibody

Description

Anti-phospho-Histone H1.4 (T17) antibody is a recombinant monoclonal antibody that recognizes the human Histone H1.4 phosphorylated at Thr17 residue. This phospho-Histone H1.4 (T17) antibody was drawn and isolated from the tissue culture supernatant (TCS) that cultivates the cell lines containing vectors of the human phospho-Histone H1.4 (T17) monoclonal antibody gene. It underwent affinity-chromatography purification. It is a rabbit IgG. And it can be used for ELISA, IHC, and IF testing with human samples.

The higher-order chromatin structure is maintained and established partially by the linker histone H1. H1.4, one of the 11 human H1 isoforms, is one of the most widely expressed somatic H1 variations, with significant levels of expression in many cells. Phosphorylation of H1 has been recognized as its most prominent modification. The antibodies against phospho-Histone H1.4 (T17) are helpful in the detection and localization of histone H1.4 phosphorylated at Thr17.

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Target Background

Function

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.

Gene References into Functions

Results show that histones H1.2 and H1.4 were observed in MDA-MB-231 metastatic breast cancer cells. The phosphorylation at S173 of histone H1.2 and S172, S187, T18, T146, and T154 of H1.4 significantly increases during M phase suggesting that these events are cell cycle-dependent. PMID:26209608
This study identified and confirmed HIST1H1E protein changes within the postsynaptic density in schizophrenia. PMID:25048004
the N-terminal domain of H1 is an important determinant of affinity and specificity of H1-chromatin interactions. PMID:22425985
PKA-mediated H1.4S35 phosphorylation dissociates H1.4 from mitotic chromatin but also suggest that this phosphorylation is necessary for specific mitotic functions. PMID:21852232
Allele-specific underacetylation of histone H4 downstream from promoter is associated with X-inactivation in human cells. PMID:12498347
the lysine residue adjacent to the phosphorylation site found on the serine residue on the H1.4 peptide KARKSAGAAKR was also shown to be methylated, raising the question of whether the hypothesized "methyl/phos" switch could be extended to linker histones PMID:15595731
Dynamic Histone H1 Isotype 4 Methylation and Demethylation by Histone Lysine Methyltransferase G9a/KMT1C and the Jumonji Domain-containing JMJD2/KDM4 Proteins PMID:19144645