Phospho-Histone H1.4 (T17) Recombinant Monoclonal Antibody

Code: CSB-RA010380A17phHU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, IHC, IF

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Product Details
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Target Background

Product Details

Uniprot NO.

P10412

Target Names

HIST1H1E

Alternative Names

H1 histone family member 4 antibody; H1.4 antibody; H14_HUMAN antibody; H1E antibody; H1F4 antibody; Hist1h1e antibody; Histone 1 H1e antibody; Histone cluster 1 H1e antibody; Histone H1 antibody; Histone H1.4 antibody; Histone H1B antibody; MGC116819 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3E1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:500
IF	1:30-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA010380A17phHU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA010380A17phHU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of MCF-7 cells with CSB-RA010380A17phHU at 1:56,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Description

Histone H1.4, encoded by the HIST1H1E gene, serves as a linker histone that plays a fundamental role in chromatin compaction and gene regulation. Phosphorylation at threonine 17 represents a dynamic post-translational modification that influences chromatin structure during cell cycle progression and transcriptional regulation, making it a valuable marker for researchers investigating epigenetic mechanisms and nuclear signaling pathways.

This recombinant monoclonal antibody, clone 3E1, offers the reproducibility and consistency that demanding epigenetic studies require. Produced using recombinant technology with a sequence-defined approach, it eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG format, purified by affinity chromatography, ensures high specificity for the phosphorylated threonine 17 epitope on human Histone H1.4.

Validation across multiple experimental platforms demonstrates this antibody's versatility in your workflow. Immunohistochemistry studies have confirmed reliable nuclear staining in paraffin-embedded human lymph node and pancreatic tissues using citrate buffer antigen retrieval at dilutions of 1:100, with a recommended working range of 1:50 to 1:500. For immunofluorescence applications, testing in MCF-7 human breast cancer cells at 1:56 dilution revealed clear nuclear localization patterns when counter-stained with DAPI, supporting its utility in cell-based epigenetic studies with recommended dilutions between 1:30 and 1:200.

Whether you are mapping phospho-histone dynamics during cell division, investigating chromatin remodeling in cancer models, or exploring transcriptional regulation mechanisms, this antibody provides a dependable tool for detecting this specific phosphorylation event in human samples across tissue-based and cellular immunostaining applications.

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Target Background

Function(From Uniprot)

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.

Gene References into Functions

Results show that histones H1.2 and H1.4 were observed in MDA-MB-231 metastatic breast cancer cells. The phosphorylation at S173 of histone H1.2 and S172, S187, T18, T146, and T154 of H1.4 significantly increases during M phase suggesting that these events are cell cycle-dependent. PMID:26209608
This study identified and confirmed HIST1H1E protein changes within the postsynaptic density in schizophrenia. PMID:25048004
the N-terminal domain of H1 is an important determinant of affinity and specificity of H1-chromatin interactions. PMID:22425985
PKA-mediated H1.4S35 phosphorylation dissociates H1.4 from mitotic chromatin but also suggest that this phosphorylation is necessary for specific mitotic functions. PMID:21852232
Allele-specific underacetylation of histone H4 downstream from promoter is associated with X-inactivation in human cells. PMID:12498347
the lysine residue adjacent to the phosphorylation site found on the serine residue on the H1.4 peptide KARKSAGAAKR was also shown to be methylated, raising the question of whether the hypothesized "methyl/phos" switch could be extended to linker histones PMID:15595731
Dynamic Histone H1 Isotype 4 Methylation and Demethylation by Histone Lysine Methyltransferase G9a/KMT1C and the Jumonji Domain-containing JMJD2/KDM4 Proteins PMID:19144645