PTK7 Recombinant Monoclonal Antibody

Code: CSB-RA622651MA1HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human, Macaca fascicularis

Application: ELISA

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Target Background

Product Details

Uniprot NO.

Q13308

Target Names

PTK7

Alternative Names

8430404F20Rik antibody; CCK 4 antibody; CCK-4 antibody; Colon carcinoma kinase 4 antibody; Human colon carcinoma kinase 4 (CCK4) mRNA complete cds antibody; Inactive tyrosine-protein kinase 7 antibody; mPTK7/CCK4 antibody; OTTHUMP00000217016 antibody; Protein tyrosine kinase PTK 7 antibody; Protein-tyrosine kinase 7 antibody; Pseudo tyrosine kinase receptor 7 antibody; PTK 7 antibody; PTK7 antibody; PTK7 protein tyrosine kinase 7 antibody; PTK7 protein tyrosine kinase antibody; PTK7_HUMAN antibody; Serum response factor antibody; Srf antibody; Tyrosine-protein kinase-like 7 antibody

Species Reactivity

Human, Macaca fascicularis

Immunogen

Recombinant Human PTK7 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

8H7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol

Recommended Dilution

Application	Recommended Dilution
ELISA	1:5000-1:50000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

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The Binding Activity of Monkey PTK7 with Anti-PTK7 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Monkey PTK7(CSB-MP5478MOV) at 2 μg/mL can bind Anti-PTK7 recombinant antibody. The EC₅₀ is 1.399 - 1.666 ng/mL.
The Binding Activity of Human PTK7 with Anti-PTK7 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human PTK7（CSB-MP622651HU2） at 2 μg/mL can bind Anti-PTK7 recombinant antibody. The EC₅₀ is 0.3675 - 0.6584 ng/mL.
The purity of PTK7 was greater than 95% as determined by SEC-HPLC

Description

PTK7, also known as protein tyrosine kinase 7 or colon carcinoma kinase 4 (CCK-4), represents a distinctive member of the receptor tyrosine kinase family characterized by its catalytically inactive kinase domain. Despite lacking enzymatic activity, PTK7 plays essential roles in planar cell polarity, Wnt signaling modulation, and cell migration processes. Its involvement in developmental pathways and aberrant expression patterns in various malignancies, including colorectal and lung cancers, has established PTK7 as a compelling target for both basic research and therapeutic development.

This recombinant monoclonal antibody, clone 8H7, offers researchers the reproducibility advantages inherent to sequence-defined antibody production. Unlike traditional hybridoma-derived antibodies that can drift over time, recombinant technology ensures that every lot delivers identical binding characteristics, providing confidence when comparing results across extended studies or between laboratories.

Functional ELISA validation demonstrates robust binding performance with cross-species reactivity spanning human and cynomolgus macaque PTK7. The antibody exhibits particularly strong affinity for human PTK7, with EC50 values in the sub-nanogram range (0.37–0.66 ng/mL), while maintaining reliable detection of monkey PTK7 at slightly higher concentrations (EC50: 1.40–1.67 ng/mL). SEC-HPLC analysis confirms greater than 95% purity, reflecting the quality achievable through affinity chromatography purification.

The broad recommended dilution range for ELISA applications (1:5,000 to 1:50,000) provides flexibility for optimization across different experimental conditions. For researchers investigating Wnt pathway regulation, cell polarity mechanisms, or exploring PTK7 as a biomarker in oncology studies, this antibody delivers the consistency and sensitivity needed for meaningful, reproducible results.

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Target Background

Function(From Uniprot)

Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.

Gene References into Functions

miR2055p is involved in the proliferation, migration and invasion of colorectal cancer cells through inhibiting PTK7. PMID:29488611
Higher expression of PTK7 significantly indicates worse prognosis in human malignancies PMID:28924970
Inhibition of PTK7 by siRNA treatment significantly decreases the viability of atypical teratoid rhabdoid tumors (ATRT)patient-derived tumor cell lines.These studies provide the groundwork for future preclinical in vivo studies aiming to investigate the efficacy of PTK7 inhibition on ATRT tumor growth PMID:28442586
PTK7 localization and protein stability is affected by canonical Wnt ligands. PMID:28420671
These findings demonstrate that PTK7 upregulates MMP9 through activation of AP-1 and NF-kappaB and, thus increases invasive properties of ESCC cells. PMID:27689325
PTK7 overexpression is associated with esophageal squamous cell carcinoma. PMID:28545451
Our data indicate that PTK7 protein expression is associated with the prognosis of oral tongue squamous cell carcinoma PMID:28547980
PTK7 expression was correlated with tumor differentiation (P=0.027), lymph node metastasis (P=0.005), distant metastasis (P=0.001) and TNM stage (P=0.028) of colorectal cancer patients PMID:27499181
High PTK7 expression is associated with radioresistance in esophageal squamous cell carcinoma. PMID:27557627
PTK7 enriches in self-renewing, multipotent stem cells of the human colon. PMID:26549850
findings implicate PTK7 as a risk factor for NTDs and provide additional evidence for a pathogenic role of PCP signaling in these malformations PMID:26368655
overexpression in PTK7-negative cancer cells led to increased metastatic events. PTK7 expression thus represents a potential prognostic biomarker and a novel therapeutic target in CRC. PMID:25962058
data demonstrates that PTK7 regulates the activity of KDR biphasically by inducing oligomerization of KDR molecules at lower concentrations and by surrounding KDR molecules at higher concentrations. PMID:25986862
PTK7 regulates Id1 expression in CD44-high glioma cell lines. Targeting PTK7 could be an effective strategy for treating glioma with high CD44 expression. PMID:25204555
The results suggest that N-acylethanolamine acid amidase and protein tyrosine kinase 7 may be used as potential tissue biomarkers to avoid overtreatment of non-aggressive prostate cancer PMID:24741114
Expression level of PTK7 was significantly decreased from benign to malignant ovarian epithelial tumors. survival analysis showed that patients with negative expression of PTK7 protein had poorer outcome than those with positive expression. PMID:25550828
PTK7 acts as a cancer-related gene and may be a potent prognostic marker for Hepatocellular carcinoma(HCC). PMID:25796105
results provide convincing evidence that both PTK7 expression and proteolysis, rather than the level of the cellular full-length PTK7 alone, contribute to efficient directional cell motility and metastasis in cancer PMID:25006253
PTK7 expression plays an important role in the invasiveness of intrahepatic cholangiocarcinoma (ICC) cells and leads to a poor prognosis in ICC patients. PMID:24587299
PTK 7 is a transforming gene and prognostic marker for breast cancer and nodal metastasis involvement. PMID:24409301
This work defines PTK7 as a highly and specifically expressed gene in adenocarcinoma and a potential therapeutic target in this subset of NSCLC. PMID:24654231
Increased expression of PTK7 was inversely correlated with overall survival. PMID:23663482
in patients treated by chemotherapy, patients with PTK7-negative tumors seemed to have better DFS than those with PTK7-positive tumors, particularly for patients treated with only anthracycline-therapy drugs PMID:24023307
PTK7 is a novel marker of epithelial to mesenchymal transitions PMID:23209741
cleavage of the PTK7 ectodomain by an ADAM proteinase was coupled with the membrane type-1 matrix metalloproteinase (MT1-MMP) cleavage of the PKP(621) downward arrowLI site in the seventh Ig-like domain of PTK7. PMID:23095747
PTK7 has utility as a biomarker for detecting minimal residual disease of T cell acute lymphoblastic leukemia in the bone marrow PMID:22898210
a novel role for PTK7 in the tumorigenesis via generation of PTK7-CTF2 by sequential cleavage of ADAM17 and gamma-secretase. PMID:22665490
aberrations in the membrane type 1 matrix metalloproteinase/PTK7 axis are detrimental to cell movements that shape the body plan PMID:21518755
Observations may indicate a role for PTK7 in cell proliferation and cell apoptosis and may provide a potential therapeutic pathway for the treatment of a variety of cancers. PMID:21103379
Protein Tyrosine Kinase 7 is required for beta-catenin-dependent transcriptional events induced by canonical Wnt ligands PMID:21132015
Results indicate that PTK7 is a major target of MT1-MMP. PMID:20837484
PTK7 is a planar cell polarity component expressed in the myeloid progenitor compartment that conveys promigratory and antiapoptotic signals into the cell and represents an independent prognosis factor in patients treated with induction chemotherapy. PMID:20558616
cloning, sequencing and expression of gene and its splice variants PMID:12427550
Lack of a thermodynamically meaningful self-association propensity for the CCK4 transmembrane domains demonstrates that the conserved GxxxG motif is not sufficient to drive CCK4 transmembrane helix-helix interactions. PMID:15683231
mechanism of PTK7 upregulation in colorectal cancer PMID:17671748
PTK7 plays an important role not only in tube formation, migration, and invasion of endothelial cells but also in angiogenesis. PMID:18471990

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Subcellular Location

Membrane; Single-pass type I membrane protein. Cell junction. Note=Colocalizes with MMP14 at cell junctions. Also localizes at the leading edge of migrating cells.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily

Tissue Specificity

Highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. But not expressed in colon.

Database Links

HGNC: 9618

UNIGENE: Hs.90572

KEGG: hsa:5754

STRING: 9606.ENSP00000230419

OMIM: 601890

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