PTK2B Recombinant Monoclonal Antibody

Code: CSB-RA585042A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human, Mouse, Rat

Application: ELISA, WB, IF, FC

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Target Background

Product Details

Uniprot NO.

Q14289

Target Names

PTK2B

Alternative Names

CADTK antibody; CAK-beta antibody; CAKB antibody; CAKbeta antibody; Calcium regulated non receptor proline rich tyrosine kinase antibody; Calcium-dependent tyrosine kinase antibody; Cell adhesion kinase beta antibody; E430023O05Rik antibody; EC 2.7.10.2 antibody; FADK 2 antibody; FADK2 antibody; FAK2 antibody; FAK2_HUMAN antibody; Focal adhesion kinase 2 antibody; MGC124628 antibody; PKB antibody; Proline-rich tyrosine kinase 2 antibody; Protein kinase B antibody; Protein Tyrosine Kinase 2 Beta antibody; Protein-tyrosine kinase 2-beta antibody; PTK antibody; PTK2B antibody; PTK2B protein tyrosine kinase 2 beta antibody; PYK2 antibody; RAFTK antibody; RAFTK2 antibody; Related adhesion focal tyrosine kinase antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human PTK2B protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

16C8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Jurkat whole cell lysate(30µg), A431 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), COLO205 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg)
All lanes:PTK2B antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 116 kDa
Observed band size: 116 kDa
Exposure time：1min
Immunofluorescence staining of A549 cell with CSB-RA585042A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing jurkat cells stained with CSB-RA585042A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Description

PTK2B, also known as PYK2 or FAK2, is a calcium-dependent non-receptor tyrosine kinase that plays a central role in signal transduction pathways governing cell adhesion, migration, proliferation, and survival. As a key mediator linking integrin and growth factor receptor signaling to downstream effectors, PTK2B is particularly important in immune cell function, neuronal signaling, and bone remodeling. Its dysregulation has been implicated in various pathological conditions, making it a valuable target for researchers investigating cellular signaling mechanisms.

This recombinant monoclonal antibody, generated from clone 16C8, offers the consistency and reproducibility that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity, ensuring that optimization efforts translate reliably across experiments and over time.

Validation studies demonstrate robust performance across multiple applications. In Western blot analysis, the antibody detects PTK2B at the expected 116 kDa molecular weight in human cell lines including Jurkat, A431, THP-1, and COLO205, as well as in mouse and rat brain tissue lysates, confirming reliable cross-species reactivity for comparative studies. Immunofluorescence staining in A549 cells reveals clear cytoplasmic localization, while flow cytometry analysis in Jurkat cells shows distinct positive signal separation from isotype controls, providing flexibility for both imaging and quantitative single-cell approaches.

The unconjugated format allows researchers to pair this antibody with their preferred detection systems. For investigators studying signal transduction, immune cell biology, or neurological processes, this antibody provides a dependable tool for interrogating PTK2B expression and localization across diverse experimental contexts.

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Target Background

Function(From Uniprot)

Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca(2+) levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.

Gene References into Functions

LFA-1 cross-linking recruits and activates FAK1 and PYK2 to phosphorylate LAT selectively on a single Y-171 site that binds to the GRB2-SKAP1 complex and limits dwell times of T-cells with dendritic cells PMID:28699640
Pyk2 has a role in spine structure and synaptic function; its deficit contributes to Huntington's disease cognitive impairments PMID:28555636
Results provide evidence that Pyk2 phosphorylates STIM1 at its Y361 residue, activating thereby store-operated Ca(2+) entry. PMID:28218251
The authors' findings identify Pyk2 as a unique mediator of invadopodium formation and function and also provide a novel insight into the mechanisms by which Pyk2 mediates tumor cell invasion. PMID:29133485
Results show that VEGFA induces Pyk2 activation in mediating human retinal microvascular endothelial cell migration, sprouting and tube formation, and that Pyk2-mediated STAT3 activation is required for hypoxia-induced retinal neovascularization. PMID:27210483
Interestingly, rs2279590 locus has a widespread enhancer effect on two nearby genes, protein tyrosine kinase 2 beta (PTK2B) and epoxide hydrolase-2 (EPHX2); both of which have been previously associated with AD as risk factors. PMID:28973302
Multiple myeloma that is driven by deregulated iron homeostasis and/or Pyk2/beta-cateninn signaling is susceptible to deferasirox-induced apoptosis. PMID:27602957
In summary, our data suggested that PYK2 via S6K1 activation modulated AR function and growth properties in prostate cancer cells. Thus, PYK2 and S6K1 may potentially serve as therapeutic targets for PCa treatment. PMID:27492635
Our findings suggest that Pyk2 plays an important role in the coordination of stabilization of beta-catenin in the crosstalk between Wnt/beta-catenin and Wnt/Ca(2+) signaling pathways upon Wnt3a stimulation in differentiating hNPCs. PMID:28694190
STIM1-induced Ca(2+) signaling activates Pyk2 to inhibit the interaction of VE-PTP and VE-cadherin and hence increase endothelial permeability. PMID:28385807
Ascites and CCL18 stimulate the phosphorylation and expression of Pyk2, which positively regulates ascites-induced ovarian cancer cell migration. PMID:27613122
We demonstrated trophoblast cytoprotection by intervention with supraphysiological concentrations of relaxin, a process in part mediated through the PI3-kinase-Akt/PKB cell survival pathway. These results provide further rationale for clinical investigation of relaxin as a potential therapeutic in preeclampsia. PMID:28122716
PTK2B polymorphism (rs28834970) could modify the risk of late-onset Alzheimer's disease (LOAD), and PTK2B polymorphism (rs28834970) and APOE may interact to increase LOAD risk in a Han Chinese population. PMID:27080426
Studies suggest that PYK2 is a common downstream effector of ErbB and IL8 receptors, and that PYK2 integrates their signaling pathways through a positive feedback loop to potentiate breast cancer invasion. PMID:26084289
Pyk2 is a key downstream signaling molecules of CCR7 in SCCHN, which promotes SCCHN tumorigenesis and progression. PMID:26352169
Phosphoproteomic analysis identifies FAK2 as a potential therapeutic target for tamoxifen resistance in breast cancer. PMID:26330541
Pyk2-focal adhesion targeting domain interacts with and binds to leupaxin. PMID:26866573
Src has a role in priming Pyk2 (but not FAK) phosphorylation and subsequent activation downstream of integrins PMID:26866924
Data strongly suggest that chemokine-stimulated associations between Vav1, SLP-76, and ADAP facilitate Rac1 activation and alpha4beta1-mediated adhesion, whereas Pyk2 opposes this adhesion by limiting Rac1 activation. PMID:26202465
FAK and PYK2 functioned redundantly to promote the Wnt/beta-catenin pathway by phosphorylating GSK3beta(Y216) to reinforce pathway output-beta-catenin accumulation and intestinal tumorigenesis. PMID:26274564
This study identified Pyk2 as a cellular component required for the intracellular trafficking of HPV16 during infection. PMID:26109718
These results suggest that melatonin exerts anti-migratory and anti-invasive effects on glioma cells in response to hypoxia via reactive oxygen species-alphavbeta3 integrin-FAK/Pyk2 signaling pathways. PMID:25889845
data suggest a novel role for FAK in GPVI-dependent ROS formation and platelet activation and elucidate a proximal signaling role for FAK within the GPVI pathway. PMID:25415317
Orai1/Pyk2 pathway is essential for glioma migration and invasion. PMID:25433371
Pyk2-NDRG1 axis is possibly involved in conveying the anti-proliferative effect of beta-ionone in prostate cancer cells. PMID:25219547
Pyk2 has a role in integrin regulation of size and dynamics of signaling microclusters PMID:25778396
PYK2 is an intermediary component of Ca(2+) signaling between PKA-mediated and Tyr phosphorylations that is required for achieving functional human sperm capacitation. PMID:25180269
Data indicate that chicken paxillin leucine-aspartate LD2 and LD4 motifs preferentially dock at the helix 2/3 binding site of human Proline-rich tyrosine kinase 2 (Pyk2)-focal adhesion kinase (FAK). PMID:25174335
these data demonstrate that Pyk2 is a critical regulator of PI3K function downstream of the TCR. PMID:25387834
Data indicate the tumor-promoting role of proline-rich tyrosine kinase 2 (Pyk2) in multiple myeloma (MM), suggesting tyrosine kinase inhibitor as a therapeutic option in MM. PMID:25217697
inhibition of FAK, PYK2 and BCL-XL synergistically enhances apoptosis in ovarian clear cell carcinoma cell lines PMID:24523919
Pyk2 and Src are important in CCL18-induced breast cancer metastasis. PMID:24142406
High expression of proline-rich tyrosine kinase 2 is associated with hepatocellular carcinoma via regulating phosphatidylinositol 3-kinase/AKT pathway. PMID:22618716
Development of a coordinated allo T cell and auto B cell response against autosomal PTK2B after allogeneic hematopoietic stem cell transplantation. PMID:24097630
Pyk2 is essential for skin wound reepithelialization in vivo and in vitro and that it regulates epidermal keratinocyte migration via a pathway that requires PKCdelta and MMP functions. PMID:24598361
Pyk2 is a shared key mediator of targeted-therapy induced adhesion and migration; Targeting Pyk2 may serve as an effective therapeutic strategy to reduce extramedullar relpase in acute promyelocytic leukemia and chronic myeloid leukemia. PMID:24176282
We hypothesize a potential direct or indirect role for SRC, RAF1, PTK2B genes in neurotransmission and in central nervous system signaling processes. PMID:24108181
Determine a crucial role of LRP1-mediated Pyk2 phosphorylation on hypoxia-induced MMP-9 activation and hVSMC migration and therefore in hypoxia-induced vascular remodeling. PMID:24072693
Both Pyk2 and phosphorylated Pyk2[pY881] are potential prognostic factors and therapeutic targets for non-small-cell lung cancer. PMID:23922106
Our results confirm that RTKs are frequently altered in chordomas. PMID:23618355
Results indicate that in vitro Pyk2 might function to regulate cell adhesion and motility following all-trans-retinoic acid (ATRA). PMID:23587524
We conclude that the effects of selenoprotein H on mitochondrial biogenesis and mitochondrial function are probably mediated through protein kinase A-CREB-PGC-1alpha and Akt/protein kinase B-CREB-PGC-1alpha pathways. PMID:23220172
Data indicate protein secretion pathways activated by monosodium urate (MSU) in macrophages, and reveal a novel role for cathepsin B and Src, Pyk2, PI3 kinases in the activation of unconventional protein secretion. PMID:23292187
These results identify a novel pathway of integrin alphaIIbbeta3 outside-in signaling and recognize the tyrosine kinase Pyk2 as a major regulator of platelet adhesion and spreading on fibrinogen. PMID:23216754
the non-catalytic functions of the kinases Fyn and Pyk2 were required for late stage human T cell adhesion. PMID:23300847
SOCS3 definitely plays roles in regulating Pyk2 signaling and cell motility in A549 cells. PMID:23302305
PAC1 regulates PYK-2 tyrosine phosphorylation in a calcium-dependent manner in lung cancer cell lines. PMID:22581436
findings showed that Pyk2 is overexpressed in squamous cell carcinoma of the head and neck; data suggest that CCR7 via Pyk2 and cofilin regulates the chemotaxis and migration ability of metastatic squamous cell carcinoma of the head and neck cells PMID:22923218
Down-regulation of both miR-517a and miR-517c contribute to hepatocellular carcinoma cells development through regulating Pyk2. PMID:23142219
Pyk2, which was strongly activated by IGF-I, was critical for IGF-IR-dependent motility. PMID:22859931

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Subcellular Location

Cytoplasm. Cytoplasm, perinuclear region. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, focal adhesion. Cell projection, lamellipodium. Cytoplasm, cell cortex. Nucleus. Note=Interaction with NPHP1 induces the membrane-association of the kinase. Colocalizes with integrins at the cell periphery.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, FAK subfamily

Tissue Specificity

Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney (at protein level). Also expressed in spleen and lymphocytes.

Database Links

HGNC: 9612

UNIGENE: Hs.491322

KEGG: hsa:2185

STRING: 9606.ENSP00000332816

OMIM: 601212

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