PRKD2 Recombinant Monoclonal Antibody

Code: CSB-RA789546A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, WB, IF, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

Q9BZL6

Target Names

PRKD2

Alternative Names

Serine/threonine-protein kinase D2 (EC 2.7.11.13) (nPKC-D2), PRKD2, PKD2

Species Reactivity

Human

Immunogen

A synthesized peptide from human PRKD2 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

23H11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: A-431 whole cell lysate(30µg), Jurkat whole cell lysate(30µg)
All lanes: PKD2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 97 kDa kDa
Observed band size: 105 kDa kDa
Exposure time：2min
Immunofluorescence staining of A431 cell with CSB-RA789546A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing K562 cells stained with CSB-RA789546A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

PRKD2, also known as protein kinase D2, is a serine/threonine kinase that plays essential roles in signal transduction pathways regulating cell proliferation, differentiation, and survival. As a member of the protein kinase D family, PRKD2 is activated by diacylglycerol and protein kinase C, positioning it as a critical node in lipid-mediated signaling cascades. Its involvement in processes ranging from Golgi membrane trafficking to oxidative stress responses makes it a compelling target for researchers investigating cancer biology, metabolic regulation, and cellular stress pathways.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human PRKD2, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences, you can expect uniform performance across lots, eliminating the variability that can complicate longitudinal studies or multi-site collaborations.

Validation across multiple platforms demonstrates this antibody's versatility in your research toolkit. Western blot analysis in A-431 and Jurkat whole cell lysates reveals a band at approximately 105 kDa, slightly higher than the predicted 97 kDa molecular weight. This size difference likely reflects post-translational modifications such as phosphorylation or glycosylation, which are characteristic of active kinases in cellular contexts. Immunofluorescence staining in A-431 cells and flow cytometry analysis in K562 cells further confirm reliable detection in both fixed and permeabilized sample preparations.

Whether you are mapping kinase signaling networks, investigating tumor cell biology, or characterizing stress response mechanisms, this antibody provides a dependable tool for detecting PRKD2 in human samples across western blotting, immunofluorescence, and flow cytometry applications.

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Target Background

Function(From Uniprot)

Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of cell proliferation via MAPK1/3 (ERK1/2) signaling, oxidative stress-induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, signaling downstream of T-cell antigen receptor (TCR) and cytokine production, and plays a role in Golgi membrane trafficking, angiogenesis, secretory granule release and cell adhesion. May potentiate mitogenesis induced by the neuropeptide bombesin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression. In response to oxidative stress, is phosphorylated at Tyr-438 and Tyr-717 by ABL1, which leads to the activation of PRKD2 without increasing its catalytic activity, and mediates activation of NF-kappa-B. In response to the activation of the gastrin receptor CCKBR, is phosphorylated at Ser-244 by CSNK1D and CSNK1E, translocates to the nucleus, phosphorylates HDAC7, leading to nuclear export of HDAC7 and inhibition of HDAC7 transcriptional repression of NR4A1/NUR77. Upon TCR stimulation, is activated independently of ZAP70, translocates from the cytoplasm to the nucleus and is required for interleukin-2 (IL2) promoter up-regulation. During adaptive immune responses, is required in peripheral T-lymphocytes for the production of the effector cytokines IL2 and IFNG after TCR engagement and for optimal induction of antibody responses to antigens. In epithelial cells stimulated with lysophosphatidic acid (LPA), is activated through a PKC-dependent pathway and mediates LPA-stimulated interleukin-8 (IL8) secretion via a NF-kappa-B-dependent pathway. During TCR-induced T-cell activation, interacts with and is activated by the tyrosine kinase LCK, which results in the activation of the NFAT transcription factors. In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane and in polarized cells is involved in the transport of proteins from the TGN to the basolateral membrane. Plays an important role in endothelial cell proliferation and migration prior to angiogenesis, partly through modulation of the expression of KDR/VEGFR2 and FGFR1, two key growth factor receptors involved in angiogenesis. In secretory pathway, is required for the release of chromogranin-A (CHGA)-containing secretory granules from the TGN. Downstream of PRKCA, plays important roles in angiotensin-2-induced monocyte adhesion to endothelial cells. Plays a regulatory role in angiogenesis and tumor growth by phosphorylating a downstream mediator CIB1 isoform 2, resulting in vascular endothelial growth factor A (VEGFA) secretion.

Gene References into Functions

the knockdown of PKD2 reduces cell death and promotes polyploidization induced by PMA. PMA/PKD2-mediated necrosis via PARP cleavage involves both SOD1-dependent and -independent pathways. PMID:26241492
The results implied that PKD2 silencing might inhibit migration of MCF7/DOX cells without affecting chemoresistance significantly PMID:25874490
PRKD2 silencing induces glioma cell senescence via p53-dependent and -independent pathways. PMID:24463355
Both PKD2 and GOLPH3 play important roles in the progression of human gliomas by promoting cell proliferation. PMID:25218347
PKD2 controls secretion of MMP7 and 9 in an isoform-specific manner PMID:24336522
PRKD2 mediates BAZF gene expression by VEGF-A stimulation. PMID:23515950
PRKD2 is a potential target to interfere with glioblastoma cell migration and invasion, two major determinants contributing to recurrence of glioblastoma after multimodality treatment. PMID:23562655
results suggest that PKD2 is involved in the regulation of PP2A activity in activated T cells through phosphorylation of Ser171 of SET PMID:23251465
found that AMAP1 had the ability to bind directly to PRKD2 and hence to make a complex with the cytoplasmic tail of the beta1 subunit PMID:22734003
upregulation of PKD2 expression may determine the behavior of gastric tumor cells, which promotes invasive phenotype and could result in general poor prognosis. PMID:22217708
Tyrosine phosphorylation of PKD2 is required for IFNalpha-stimulated activation of this kinase as well as for efficient serine phosphorylation and degradation of IFNAR1 and ensuing restriction of the extent of cellular responses to IFNalpha. PMID:21865166
PKD2 is a common signaling target downstream of various agonist receptors in platelets and G(q)-mediated signals along with calcium and novel PKC isoforms, in particular, PKCdelta activate PKD2 in platelets. PMID:21736870
Expression and kinase activity of PKD2 are required for the ligand-inducible stimulation of IFNAR1 ubiquitination and endocytosis and for accelerated proteolytic turnover of IFNAR1. PMID:21173164
PKD2 regulates hypoxia-induced VEGF-A expression/secretion by tumour cells and VEGF-A stimulated blood vessel formation and a essential mediator of tumour cell-endothelial cell communication and a target to inhibit angiogenesis in gastrointestinal cancers PMID:20732914
mechanism of activation by CCK(B)/gastrin receptor PMID:12058027
Data show that Bcr-Abl-induced activation of the nuclear factor kappaB cascade in myeloid leukemia cells is largely mediated by tyrosine-phosphorylated PKD2. PMID:15604256
Critical components of the PKD2 regulatory domain control phorbol ester binding, catalytic activity, and nucleocytoplasmic shuttling and reveal similarities but also striking differences compared to the published data for this domain in PKD1. PMID:15975900
PKD2 plays an important role in carcinoid tumor progression. PMID:16899224
PKD2 is involved in the production of IL-8, a potent proinflammatory chemokine, by epithelial cells. PMID:16928771
The data suggest that PKD2 is involved in IL-2 promoter regulation and cell death depending on its activity upon TCR stimulation in Jurkat cells. PMID:17077180
Neurotensin induces a striking increase in Hsp27 phosphorylation on Ser-82 in PANC-1 cells through convergent p38 MAPK, PKD, and PKD2 signaling. PMID:17570131
stimulation of P-selectin surface expression via PKCalpha-dependent PKD2 activation could be an important mechanism in the early onset of AII-initiated endothelial adhesiveness PMID:17951978
Signal transduction pathway is defined downstream of CCK2 receptor showing that CK1 delta and epsilon phosphorylate PKD2 at 3 sites, resulting in nuclear accumulation of PKD2 and phosphorylation of nuclear PKD2 substrates in human gastric cancer cells. PMID:17962809
expression and catalytic activity of PKD2 are required for the release of chromogranin A containing secretory vesicles PMID:18262756
PKD2 plays a pivotal role in endothelial cell proliferation and migration necessary for angiogenesis at least in part through modulation of the expression of vascular endothelial growth factor receptor-2 and fibroblast growth factor receptor-1 PMID:19001381
Lck regulated the activity of PKD2 by tyrosine phosphorylation, which in turn may have modulated the physiological functions of PKD2 during T cell receptor-induced T cell activation. PMID:19192391
Lysophosphatidylcholine activates a novel PKD2-mediated signaling pathway that controls monocyte migration. PMID:19520973
a transient association of PKD1 and PKD2 with NHERF-1 in live cells that is triggered by phorbol ester stimulation and, importantly, differs strikingly from the sustained translocation to plasma membrane. PMID:19581308