PRKAR2B Recombinant Monoclonal Antibody

Code: CSB-RA573401A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, FC

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Target Background

Product Details

Uniprot NO.

P31323

Target Names

PRKAR2B

Alternative Names

AI451071 antibody; AW061005 antibody; cAMP dependent protein kinase type II beta regulatory chain antibody; cAMP dependent protein kinase type II beta regulatory subunit antibody; cAMP-dependent protein kinase type II-beta regulatory subunit antibody; H RG363E19.2 antibody; KAP3_HUMAN antibody; MGC116401 antibody; Pkarb2 antibody; PRKAR 2 antibody; PRKAR2 antibody; PRKAR2B antibody; Protein kinase cAMP dependent regulatory type II beta antibody; RATDNA antibody; RII beta antibody; RII(beta) antibody; RIIbeta antibody; WUGSC:H RG363E19.2 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human PRKAR2B

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6D12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Overlay Peak curve showing jurkat cells stained with CSB-RA573401A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

PRKAR2B encodes the type II-beta regulatory subunit of cAMP-dependent protein kinase (PKA), a central mediator of cellular responses to hormonal and neurotransmitter signals. As a key component of the PKA holoenzyme, PRKAR2B governs the spatial and temporal regulation of kinase activity by sequestering catalytic subunits until cAMP binding triggers their release. This regulatory mechanism places PRKAR2B at the intersection of metabolic control, synaptic plasticity, and endocrine signaling pathways, making it a compelling target for researchers investigating signal transduction networks and their dysregulation in disease states.

This recombinant monoclonal antibody, clone 6D12, offers the reproducibility and molecular definition that demanding experimental workflows require. Developed in rabbit against a synthetic peptide derived from human PRKAR2B, the antibody undergoes affinity chromatography purification to ensure consistent performance across experiments. The recombinant production platform eliminates the lot-to-lot variability inherent to traditional hybridoma-derived antibodies, providing researchers with a sequence-defined reagent they can rely on for longitudinal studies and cross-laboratory comparisons.

Validation for flow cytometry demonstrates clear detection of PRKAR2B in Jurkat cells, a human T lymphocyte line, with recommended working dilutions between 1:50 and 1:200. The intracellular staining protocol employed formaldehyde fixation and Triton X-100 permeabilization, confirming the antibody's suitability for detecting this cytoplasmic target in fixed cell preparations. The antibody is also validated for ELISA applications, offering flexibility for researchers requiring both single-cell and population-level analyses.

This antibody serves investigators exploring PKA signaling dynamics, cAMP-mediated pathways, and the regulatory mechanisms underlying cellular responses to extracellular stimuli.

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Target Background

Function(From Uniprot)

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

Gene References into Functions

Study identified the overexpression of PRKAR2B in castration-resistant prostate cancer (CRPC) mouse models and patients, and showed it promoted CRPC cell proliferation, invasion and survival by mainly modulates cell cycle gene expression. These results provide evidence that PRKAR2B is a novel oncogenic gene in CRPC. PMID:28008150
Release of pRIIbeta in the presence of cAMP is reduced by calcium, whereas autophosphorylation at the phosphorylation site inhibits holoenzyme reassociation with the catalytic subunit. PMID:26158466
Because of limited power, PRKAR2B's role in antipsychotic-induced weight gain is unclear, but biological evidence suggests that PRKAR2B may be involved PMID:24737441
Meta-analysis and genome-wide association study of gene-disease association. (HuGE Navigator) PMID:21068099
Clinical trial and genome-wide association study of gene-disease association. (HuGE Navigator) PMID:20195266
lipolytic catecholamine resistance of sc adipocytes in polycystic ovary syndrome is probably due to a combination of decreased amounts of beta(2)-adrenergic receptors, the regulatory II beta-component of protein kinase A, and hormone-sensitive lipase PMID:12727985
Nuclear RII beta can act as a repressor of CREB transcriptional activity in T cells, providing a potential functional significance for aberrant levels of nuclear RII beta in systemic lupus erythematosus T cells. PMID:14500661
there are abnormalities in [3H]cAMP binding and catalytic activity kinase A in brain of depressed suicide victims, which could be due to reduced expression of RIIbeta and Cbeta PMID:14744463
serine 114 phosphorylation and nuclear localization of RIIbeta controls the regulation of IL-2 gene expression in T cells. PMID:15187164
Loss of PRKAR2B protein due to a post-transcriptional mechanism in ACA-S is a new mechanism of cAMP pathway dysregulation in adrenocortical tumorigenesis. PMID:18505904
PKA RII(beta) is responsible for increased glucocorticoid sensitivity, critical for cAMP-mediated synergistic cell killing in CEM cells PMID:18544528
both the constitutive and cAMP-induced release of TNFR1 exosome-like vesicles occur via PKA-dependent pathways that are regulated by the anchoring of RIIbeta to BIG2 via AKAP domains B and C PMID:18625701

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Subcellular Location

Cytoplasm. Cell membrane. Note=Colocalizes with PJA2 in the cytoplasm and at the cell membrane.

Protein Families

CAMP-dependent kinase regulatory chain family

Tissue Specificity

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Database Links

HGNC: 9392

UNIGENE: Hs.433068

KEGG: hsa:5577

STRING: 9606.ENSP00000265717

OMIM: 176912

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