PLCG2 Recombinant Monoclonal Antibody

Code: CSB-RA028490A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, IHC, IF

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Product Details
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Target Background

Product Details

Uniprot NO.

P16885

Target Names

PLCG2

Alternative Names

1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody; 1-phosphatidylinositol-4 antibody; 5-bisphosphate phosphodiesterase gamma-2 antibody; EC 3.1.4.11 antibody; Phosphoinositide phospholipase C antibody; Phosphoinositide phospholipase C-gamma-2 antibody; Phospholipase C gamma 2 antibody; Phospholipase C, gamma 2 (phosphatidylinositol specific) antibody; Phospholipase C-gamma-2 antibody; Phospholipase C-IV antibody; PLC 2 antibody; PLC gamma 2 antibody; PLC IV antibody; PLC-gamma-2 antibody; PLC-IV antibody; Plcg2 antibody; PLCG2_HUMAN antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human PLCG2 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

11D5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA028490A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA028490A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of Hela cell with CSB-RA028490A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Description

Phospholipase C gamma 2 (PLCG2) serves as a critical signaling enzyme that catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate, generating second messengers essential for calcium mobilization and protein kinase C activation. This enzyme plays particularly important roles in immune cell signaling and has emerged as a significant focus in cardiovascular research, where its involvement in platelet activation and vascular function continues to be explored.

This recombinant monoclonal antibody against human PLCG2 offers the consistency and reliability that demanding experimental workflows require. Developed using recombinant technology with clone 11D5, this antibody provides sequence-defined specificity that ensures reproducible results across experiments and eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies. The rabbit IgG format, raised against a synthesized peptide from human PLCG2, delivers high-affinity binding suitable for multiple detection platforms.

Validation studies demonstrate robust performance across immunohistochemistry and immunofluorescence applications. In IHC, the antibody successfully detects PLCG2 in paraffin-embedded human tonsil tissue and human liver cancer samples at 1:100 dilution using citrate buffer antigen retrieval, providing researchers flexibility when examining both normal and pathological specimens. For cellular localization studies, immunofluorescence staining in HeLa cells at 1:50 dilution reveals clear signal when paired with Alexa Fluor 488-conjugated secondary antibodies, enabling detailed subcellular distribution analysis.

The unconjugated format allows researchers to select detection systems best suited to their experimental design, whether for chromogenic IHC visualization or fluorescent microscopy applications. For investigators studying PLCG2-mediated signaling in cardiovascular biology, immune cell function, or cancer research, this antibody provides a dependable tool for advancing understanding of this essential phospholipase.

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Target Background

Function(From Uniprot)

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.

Gene References into Functions

finding that mutations or polymorphisms in two putative calcium-regulated domains of PLCG2 are associated with ibrutinib-resistant CLL adds to the evidence supporting complex regulatory shifts in the PLCG2 protein likely occurring during the development of resistance PMID:28366935
The results suggest a new mechanism of PLCgamma activation with unique thermodynamic features and assign a novel regulatory role to its spPH domain. PMID:27196803
Data show that protein-altering changes are in PLCG2, ABI3, and TREM2 genes highly expressed in microglia and highlight an immune-related protein-protein interaction network in Alzheimer's disease. PMID:28714976
Ocular manifestations of phospholipase-Cgamma2-associated antibody deficiency and immune dysregulation show mutations in the PLC[gamma]2 gene leading to aberrant function of immune cells and overproduction of interleukin-1 [beta] (IL-1[beta]). PMID:27442322
R665W and L845F be referred to as allomorphic rather than hypermorphic mutations of PLCG2 Rerouting of the transmembrane signals emanating from BCR and converging on PLCgamma2 through Rac in ibrutinib-resistant CLL cells may provide novel drug treatment strategies to overcome ibrutinib resistance mediated by PLCG2 mutations or to prevent its development in ibrutinib-treated CLL patients. PMID:27542411
Data show that phospholipase Cgamma2 (PLCgamma2) is strongly expressed in B cell non-Hodgkin lymphoma and especially in a large subset of Diffuse large B-cell lymphoma (DLBCL). PMID:25012946
Characterization of the effect of missense point-mutation at R665W in PLCG2 on signaling mechanisms of ibrutinib resistance in chronic lymphocytic leukemia cells. PMID:25972157
amarogentin prevents platelet activation through the inhibition of PLC gamma2-PKC cascade and MAPK pathway PMID:24868545
PLCG2 missense mutation is a risk factor in the development of steroid sensitive nephrotic syndrome in childhood. PMID:25349203
The autoinhibitory C-terminal SH2 domain of phospholipase C-gamma2 stabilizes B cell receptor signalosome assembly. PMID:25227611
The relationship between upstream tyrosine kinase SYK and its target, PLCgamma2, is maximally predictive and sufficient to distinguish chronic lymphocytic leukemia from healthy controls. PMID:24489640
Single-nucleotide polymorphisms in PLCG2 gene is associated with breast cancer risk after menopausal hormone replacement therapy. PMID:24080446
identified three distinct mutations in PLCgamma2 in two patients resistant to ibrutinib PMID:24869598
early Ca(2+) fluxing provides feed-forward signal amplification by promoting anchoring of the PLCgamma2 C2 domain to phospho-SLP65. PMID:24166973
BANK1 and BLK have roles in B-cell signaling through phospholipase C gamma 2 PMID:23555801
down-regulation of PLCgamma2-beta-catenin pathway occurs in mice and humans and leads to myeloid-derived suppressor cells-mediated tumor expansion. PMID:24127488
Associations between treatment response and Lyn, Syk, PLCgamma2 and ERK were not found. PMID:23039362
Overexpression of the altered p.Ser707Tyr protein and ex vivo experiments using affected individuals' leukocytes showed clearly enhanced PLCgamma2 activity. PMID:23000145
PLCgamma2 participates in T cell receptor (TCR) signal transduction and plays a role in T cell selection in a transgenic mouse model. PMID:22837484
Genomic deletions in PLCG2 cause gain of PLCgamma(2) function, leading to signaling abnormalities in multiple leukocyte subsets and a phenotype encompassing both excessive and deficient immune function. PMID:22236196
Data indicate a role for PLCgamma2 and Ca(2+) signaling through the modulation of MEK/ERK in IL3/GM-csf stimulated human hematopoietic stem/progenitor cells. PMID:21506110
Rac2 binding in the absence of lipid surfaces was not able to activate phospholipase C gamma 2. PMID:21245382
SYK, together with phospholipase Cgamma2, may serve as potential biomarkers to predict dasatinib therapeutic response in patients. PMID:20068106
Data show that bile acid reflux present in patients with BE may increase reactive oxygen species production and cell proliferation via activation of PI-PLCgamma2, ERK2 MAP kinase, and NADPH oxidase NOX5-S, thereby contributing to the development of EA. PMID:20086178
Data show that RTX treatment results in a time-dependent inhibition of the BCR-signaling cascade involving Lyn, Syk, PLC gamma 2, Akt, and ERK, and calcium mobilization. PMID:19965664
collagen receptor glycoprotein VI and alphaIIbbeta3 trigger distinct patterns of receptor signalling in platelets, leading to tyrosine phosphorylation of PLCgamma2 (integrin alphaiibbeta3) PMID:12049640
Two tyrosine residues in regulating the activity of PLCgamma2 PMID:12181444
full-length cDNA for human PLCgamma2 and expressed it in E. coli using the expression vector pT5T PMID:12359094
PLCG2 has a signaling role in platelet glycoprotein Ib alpha calcium flux and cytoskeletal reorganization PMID:12813055
in gastric cancer, protein translocation of PLCgamma2 and PKCalpha is critical event in the process of apoptosis induction. PMID:14606067
PLC-gamma2 is phosphorylated on Y753, Y759, and Y1217 in response to engagement of the B-cell receptor PMID:15509800
The PLCgamma2 is present in the majority of mediastinal B cell lymphomas. PMID:15744341
novel mechanism of PLCgamma(2) activation by Rac GTPases involving neither protein tyrosine phosphorylation nor PI3K-mediated generation of PtdInsP(3) PMID:16172125
intracellular mediators and pathways activated by leptin downstream of JAK2 were found to include phosphatidylinositol-3 kinase, phospholipase Cgamma2 and protein kinase C, as well as the p38 MAP kinase-phospholipase A(2) axis. PMID:18000612
Plasmacytoid dendritic cells express a signalosome consisting of Lyn, Syk, Btk, Slp65 (Blnk) and PLCgamma2. Triggering CD303 leads to tyrosine phosphorylation of Syk, Slp65, PLCgamma2 & cytoskeletal proteins. PMID:18022864
rac regulates its effector phospholipase Cgamma2 through interaction with a split pleckstrin homology domain PMID:18728011