PIM1 Recombinant Monoclonal Antibody

Code: CSB-RA121260A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, WB, IHC

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Target Background

Product Details

Uniprot NO.

P11309

Target Names

PIM1

Alternative Names

Oncogene PIM 1 antibody; Oncogene PIM1 antibody; PIM 1 antibody; pim 1 kinase 44 kDa isoform antibody; Pim 1 kinase antibody; pim 1 oncogene (proviral integration site 1) antibody; Pim 1 oncogene antibody; PIM antibody; PIM1 antibody; pim1 kinase 44 kDa isoform antibody; PIM1_HUMAN antibody; Pim2 antibody; PIM3 antibody; Proto oncogene serine/threonine protein kinase Pim 1 antibody; Proto-oncogene serine/threonine-protein kinase Pim-1 antibody; Proviral integration site 1 antibody; Proviral integration site 2 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human PIM1 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

21C6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
, Positive WB detected in:COLO205 whole cell lysate(30µg), A549 whole cell lysate(30µg), THP-1 whole cell lysate(30µg)
All lanes: PIM1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 36 kDa
Observed band size: 32 kDa
Exposure time：300s
IHC image of CSB-RA121260A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA121260A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Description

PIM1 is a serine/threonine kinase that plays a central role in cell survival, proliferation, and oncogenic signaling pathways. As a proto-oncogene frequently overexpressed in hematological malignancies and solid tumors, PIM1 has emerged as an important therapeutic target and biomarker in cancer research. Its involvement in regulating apoptosis, cell cycle progression, and drug resistance makes it a compelling focus for investigators studying signal transduction mechanisms and tumor biology.

This recombinant monoclonal antibody, clone 21C6, offers the consistency and reliability that demanding experimental workflows require. Developed using recombinant technology with a defined sequence, this rabbit IgG provides lot-to-lot reproducibility that traditional hybridoma-derived antibodies cannot match. Researchers can confidently integrate this antibody into long-term studies knowing that performance characteristics will remain stable across different production batches.

Validation studies demonstrate robust performance across multiple applications. In western blot analysis, the antibody successfully detects PIM1 in human cell lines including COLO205, A549, and THP-1 whole cell lysates at dilutions ranging from 1:500 to 1:5000. The observed band at approximately 32 kDa appears slightly smaller than the predicted 36 kDa molecular weight, which likely reflects post-translational processing or alternative isoform expression characteristic of PIM1 biology. For immunohistochemistry applications, the antibody has been validated in paraffin-embedded human kidney tissue and liver cancer specimens at 1:50 to 1:200 dilutions using citrate buffer antigen retrieval, providing clear visualization of PIM1 expression patterns in both normal and malignant contexts.

This antibody serves researchers investigating oncogenic signaling, cell survival mechanisms, and cancer biomarker expression, supporting studies from basic mechanistic research through translational applications.

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Target Background

Function(From Uniprot)

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Phosphorylation of MAP3K5, another proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promotes cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels. Phosphorylation of CDKN1B, induces 14-3-3 proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis. Also phosphorylates and activates the ATP-binding cassette transporter ABCG2, allowing resistance to drugs through their excretion from cells. Promotes brown adipocyte differentiation.

Gene References into Functions

CD95-mediated apoptosis induces Pim-1 down-regulation in Burkitt's lymphoma (BL) B-cells, but Pim-1 down-regulation cannot fully eradicate BL and leukaemia. PMID:27641442
Data show that pim-1 oncogene protein (PIM1) expression was elevated in T-cell lymphomas (TCLs) cells. PMID:30020405
Results provide evidence that glucose deprivation is one of the mechanisms that leads to elevated Pim1 expression in colorectal cancer (CRC), and Pim1 upregulation ensures CRC growth in response to glucose deprivation by facilitating the Warburg effect in a compensatory way. PMID:29516572
data suggest that PIM1/2 kinase overexpression is a common feature of male reproductive organs tumors, which provoke tissue alterations and a large inflammatory response that may act synergistically during the process of tumorigenesis. PMID:27901106
PIM-1 mRNA levels may be an independent prognostic factor in acute myeloid leukemia. PMID:28851457
PIM1 role in cell proliferation, migration and apoptosis in triple-negative breast cancer [review] PMID:28721678
may contribute to placental inflammation in pregnancies complicated by maternal obesity PMID:28487013
Inhibition of PIM1 kinase attenuates inflammation-induced pro-labor mediators in human fetal membranes in vitro. PMID:28333279
PIM1 destabilization is associated with cancer. PMID:26993775
The Ser/Thr-protein kinase-1 (PIM-1) was identified as a direct target of miR-328. PMID:27448984
Data show that PIM1 contributes to melanoma cell proliferation and tumor growth in vivo; however, the presence of PIM2 and PIM3 could also influence the outcome. PMID:27448973
High expression level of PIM is associated with neoplasms. PMID:26956053
This review summarizes effects of PIM kinases and their substrates especially on cancer cell migration, invasion and metastatic growth, based on data from cell-based assays, animal experiments and patients. PMID:29108877
Results show that PIM-1 is upregulated in pancreatic cancer tissues and plasma. Its knockdown in pancreatic cancer cells suppressed proliferation, induced cell cycle arrest, enhanced apoptosis, resensitized cells to gemcitabine and erlotinib treatment, and inhibited ABCG2 and EZH2 mRNA expression. PMID:27596051
Results show that PIM1 is overexpressed in breast cancer tumors and provide evidence for its role in tumor resistance to PI3K inhibitors. PMID:27604488
These results demonstrate the involvement of PIM kinases in LIF-induced regulation in different trophoblastic cell lines which may indicate similar functions in primary cells. PMID:28729093
Down-regulation of UHRF1 is an important mechanism of PIM1-mediated cellular senescence. PMID:28394343
PIM kinases in classical Hodgkin lymphoma exhibit pleiotropic effects, orchestrating tumor immune escape and supporting Reed-Sternberg cell survival. PMID:28698206
critical for the growth and metastasis of osteosarcoma cells PMID:26687194
Triple negative breast cancer cells, but not nonmalignant mammary epithelial cells, were dependent on PIM1 for proliferation and protection from apoptosis. PMID:27775704
PIM1 expression was higher in triple negative breast tumors than in estrogen and progesterone receptor positive tumors. PMID:27775705
High PIM1 expression is associated with osteosarcoma. PMID:27826617
downregulation of PIM1 led to suppression of cell proliferation by cell cycle arrest at G1 phase and suppression of cell invasion and migration. PMID:28197633
Pim-1L protects hepatic ABCA1 from lysosomal degradation by facilitating the physical interaction between ABCA1 and liver X receptor beta and subsequent stabilization of the ABCA1-Pim-1L complex and thereby regulates the circulating level of high-density lipoprotein. PMID:27765770
Furthermore, the Pim-1-HBP1 positive feedback loop exerts its effect by regulating the senescence markers DNMT1 and p16 and the apoptosis marker Bax. The Pim-1-HBP1 axis thus constitutes a novel checkpoint pathway critical for the inhibition of tumorigenesis. PMID:28348080
Overexpression of PIM1 partially rescued miR-542-3p-mediated suppression of cell migration, invasion and EMT. Our results collectively indicate that miR-542-3p serves as a metastasis suppressor in melanoma, supporting its utility as a promising therapeutic candidate. PMID:27107696
Data show that cytoplasmic irradiation mediate expression level of Pim-1, which lead to glycolytic shift in SAE cells. PMID:28170315
Pim1 role in the apoptosis and cell proliferation of human esophageal cancer cells PMID:27983525
findings aid in understanding the tumor-suppressive role of miR-124-3p in astrocytoma pathogenesis through the inhibition of PIM1 translation PMID:27088547
hypoxia induced miR-124 and miR-144 downregulation may contribute to a prosurvival mechanism of prostate cancer cells to hypoxia and irradiation at least through attenuated suppressing of PIM1. PMID:26990493
Results show loss of miR-1 and miR-214 expression and high expression of their target gene, PIM1, in malignant mesothelioma suggesting a role in carcinogenesis of mesothelioma. PMID:26820394
we examine the therapeutic implications of Pim1 to encourage the personalization of cardiac regenerative therapy PMID:26563999
Data suggest that combining PIM and JAK2 kinase inhibitors may offer a more efficacious therapeutic approach for myeloproliferative neoplasms (MPNs) over JAK2 inhibitor mono-therapy. PMID:26472029
A high percentage of urothelial carcinomas express Pim kinases. Pim expression differs in NILG, NIHG, and IHG lesions. PMID:26551340
Downregulation of microRNA33a promotes the expression CDK6, CCND1, and PIM1, and gastric cancer cell proliferation. PMID:26352175
glycogen synthase kinase 3 beta (GSK3B) and the forkhead box P3 (FOXP3) transcription factors are direct PIM1 targets. PMID:26934497
By associating with PIM-1L, CD180 can thus obtain autonomous signaling capabilities, and this complex is then channeling inflammatory signals into B cell survival programs PMID:26555723
Pim1 kinase activity maintains airway epithelial integrity and protects against house dust mite-induced proinflammatory activation of the airway epithelium. PMID:26453516
Pim-1 and Pim-3 enhance phosphorylation and cell surface expression of CXCR4 in prostate cancer cells. PC-3 prostate cancer cells overexpressing either Pim-1 or Pim-3 kinases form larger xenograft tumors than the parental PC-3 cells. PMID:26075720
PIM1 is up-regulated by hypoxia in hepatocellular carcinoma and promotes tumor growth and metastasis by facilitating cancer cell glycolysis. PMID:25834102
This study demonstrates the oncogenic role of Pim-1 in ACC. The findings also suggest that Pim-1 may serve as a neoteric therapeutic target and potential prognostic marker for ACC cancer PMID:25551195
We now demonstrate a molecular mechanism which reveals a direct role for EBNA3C in enhancing Pim-1 expression in EBV-infected primary B-cells. PMID:25121590
Pim kinase may represent a new host factor for HCV entry. Pim1 is an oncogenic serine/threonine kinase. HCV NS5A protein physically interacts with Pim1 and contributes to Pim1 protein stability. PMID:26202252
PIM1 overexpression is associated with prostate cancer. PMID:24771642
Molecular dynamics studies showed that only GTP decreases the formation of the GBP1:PIM1 complex through an allosteric mechanism, outlining the rational for the identification of new compounds potentially able to revert resistance to paclitaxel. PMID:25081641
important role in progression of pre-malignant high grade prostatic intra-epithelial neoplasia to malignant prostatic carcinomas [review] PMID:25553374
In comparison with normal brain, a strong upregulation of Pim1 was demonstrated in human GBM samples. Notably, patients with short overall survival showed a significantly higher Pim1 expression compared with GBM patients who lived longer than the median. PMID:25155357
Pim1 function depends upon intracellular localization in human cardiac progenitor cells PMID:25882843
These results point on PIM1 as a novel factor in regulation of the phenotype and differentiation of fibroblasts in prostate cancer. PMID:25451079
Data indicate that serine/threonine-protein kinase PIM1 expression was noted in each case of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL). PMID:24547709