PIK3R3 Recombinant Monoclonal Antibody

Code: CSB-RA791880A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human, Mouse, Rat

Application: ELISA, WB, IHC, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

Q92569

Target Names

PIK3R3

Alternative Names

DKFZp686P05226 antibody; FLJ41892 antibody; OTTHUMP00000009783 antibody; OTTHUMP00000009786 antibody; p55 antibody; p55 gamma antibody; P55G_HUMAN antibody; p55PIK antibody; Phosphatidylinositol 3 kinase regulatory subunit gamma antibody; Phosphatidylinositol 3 kinase regulatory subunit polypeptide 3 antibody; Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55, gamma) antibody; Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma antibody; Phosphatidylinositol 3-kinase regulatory subunit gamma antibody; Phosphoinositide 3 kinase regulatory subunit 3 (gamma) antibody; Phosphoinositide 3 kinase regulatory subunit 3 antibody; Phosphoinositide 3 kinase regulatory subunit polypeptide 3 antibody; Phosphoinositide 3 kinase, regulatory subunit 3 (p55, gamma) antibody; Phosphoinositide 3 kinase, regulatory subunit, polypeptide 3 (p55, gamma) antibody; PI3 kinase p85 subunit gamma antibody; PI3-kinase regulatory subunit gamma antibody; PI3-kinase subunit p55-gamma antibody; PI3K regulatory subunit gamma antibody; Pik3r3 antibody; PtdIns 3 kinase p85 gamma antibody; PtdIns-3-kinase regulatory subunit gamma antibody; PtdIns-3-kinase regulatory subunit p55-gamma antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human PIK3R3 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2E2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
, Positive WB detected in:Mouse brain tissue lysate(30µg), Rat brain lysate(30µg)
All lanes: PI3 kinase p55 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time：120s
IHC image of CSB-RA791880A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing MCF-7 cells stained with CSB-RA791880A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Description

PIK3R3, also known as the p55 gamma regulatory subunit of phosphatidylinositol 3-kinase, plays a critical role in the PI3K signaling pathway that governs cell growth, proliferation, and survival. As a regulatory subunit, PIK3R3 modulates the activity of the catalytic subunit, making it an important target for researchers investigating signal transduction mechanisms, metabolic regulation, and oncogenic pathways where PI3K signaling is frequently dysregulated.

This recombinant monoclonal antibody, clone 2E2, offers the consistency and reproducibility that demanding experimental workflows require. Generated against a synthetic peptide derived from human PIK3R3, the antibody is produced using recombinant technology, ensuring sequence-defined specificity and eliminating the lot-to-lot variability that can compromise longitudinal studies. The rabbit IgG format provides excellent signal-to-noise characteristics across multiple detection platforms.

Validation studies demonstrate reliable performance across diverse applications. Western blot analysis of mouse and rat brain tissue lysates reveals clean detection at the expected 54 kDa molecular weight, confirming accurate target recognition. The antibody also performs well in immunohistochemistry, as shown in paraffin-embedded human lung cancer tissue sections, and in flow cytometry applications using MCF-7 cells, a breast cancer cell line commonly employed in PI3K pathway research. This cross-species reactivity across human, mouse, and rat samples provides flexibility for researchers working with different model systems.

Whether you are characterizing PI3K complex assembly, investigating pathway activation in cancer models, or examining tissue-specific expression patterns, this antibody delivers the specificity and versatility needed for rigorous signal transduction research.

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Target Background

Function(From Uniprot)

Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.

Gene References into Functions

Results showed that PIK3R3 was upregulated in colorectal cancer (CRC) tissues and inversely correlated with miR-411 expression. Its expression is regulated by miR-411 which targets its 3'UTR. PMID:29207180
PIK3R3 could promote the metastasis of Pancreatic Cancer by facilitating ZEB1 induced Epithelial-Mesenchymal Transition. PMID:29719293
Using case-control study and functional analyses, study have identified SNP rs7536272 A > G, located in the promoter region of PIK3R3, acts as a risk factor of GC through enhancing the transcriptional activity of PIK3R3, contributing to increased expression of PIK3R3. PMID:29802999
The findings suggest that miR-212 acts as a tumor suppressor in colorectal cancer by directly targeting PIK3R3. PMID:28944887
miR-181a2/181b2 prominently dampened cell-cycle progression, suppressed cell growth, and promoted apoptosis of tumor cells in vitro They also effectively impeded tumor formation and growth in vivo miR-181a2/181b2 exert the tumor suppressor ability by depressing the direct target PIK3R3 (p55gamma) and consequently modulating the PIK3R3/Akt/FoxO signaling pathway PMID:27503199
p55PIK significantly stimulated the expression of AFP by activating NF-kappaB signaling pathway in hepatocellular carcinoma cells. PMID:28970114
Study showed that PIK3R3 was a target of miR-365 and its expression was upregulated and inversely correlated with miR-365 expression in glioma tissues. PMID:28260020
PIK3R3 is a direct and functional downstream target of miR-152. PIK3R3 expression was upregulated and was inversely correlated with miR-152 expression in clinical CRC tissues. Downregulation of PIK3R3 mimicked the tumor-suppressive effects of miR-152 overexpression in CRC cells. PMID:26820128
p55PIK-PI3K signaling can contribute to imatinib resistance in gastrointestinal stromal tumors by increasing KIT expression. PMID:26587973
The proliferation, migration and invasion was decreased in ovarian SKOV3 when HOTAIR or PIK3R3 was silenced. PMID:26826873
PIK3R3 was identified as a crucial target gene of miR-132. PMID:26252738
our findings provide new insights into tumor suppression by miR-511 by negatively regulating the PIK3R3/AKT/mTOR signaling pathway PMID:25608840
p55PIK could be a substrate of activated caspase 6 during paraquat-induced apoptosis, leading to loss of original biological functions and redistribution to disturb cell cycle progression. PMID:24130211
TGF-beta/NKX2.1/PIK3R3 axis is crucial in the TGF-beta-induced inhibition of cell proliferation, and the NKX2.1/PIK3R3 axis might become a target in TGF-beta receptor-repressed lung adenocarcinoma PMID:25371235
p55gamma sequentially up-regulated p53 and p21, resulting in cell-cycle arrest in S phase; small-interfering RNA knockdown of either p53 or p21 blocked p55gamma-induced vascular smooth muscle cell growth arrest. PMID:25388664
Overexpression of PIK3R3 depends on SNAI2, inducing significant epithelial-to-mesenchymal transition (EMT). PMID:24837077
discovered that ERBB4 and S6K2 were the direct targets of miR-193a-3p and that PIK3R3 and mTOR were the direct targets of miR-193a-5p in non-small-cell lung cancer PMID:24469061
study shows that the p53/miR-148b/p55PIK axis has an important role in cell proliferation and tumor growth, and may represent a novel therapeutic target for treating cancers containing p53 mutations or losses. PMID:24632606
PIK3R3, ITGB1, ITGAL, and ITGA6, were involved in the regulation of actin cytoskeleton that link to triple-negative breast cancer migration. PMID:24982892
Containing the N24 of PI3K-p55PIK. PMID:23904382
our results show that the-PCNA interaction is important in regulating DNA synthesis and contributes to tumorigenesis. PMID:23939377
Data show that miR-7 inhibits the effects of TLR9 signaling on lung cancer cells through regulation of the PIK3R3/Akt pathway. PMID:23135998
PIK3R3 was up-regulated in gastric cancer and promoted cell cycle progression and proliferation. PMID:22876838
A fusion peptide containing the p55PIK amino terminus, TAT-N24, which not only inhibited cell proliferation, but also stimulated differentiation. PMID:22722333
IGF2-PIK3R3 signaling axis is involved in promoting the growth of a subclass of highly aggressive human glioblastomas that lack EGF receptor amplification. PMID:17360667
We have identified novel nonclassical mediators of the SREBP-1 response, including p55gamma, supporting the hypothesis that SREBP-1 regulates stress response and signaling genes. PMID:17452746