PDHB Recombinant Monoclonal Antibody

Code: CSB-RA616398A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human, Mouse, Rat

Application: ELISA, WB

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Target Background

Product Details

Uniprot NO.

P11177

Target Names

PDHB

Alternative Names

DKFZp564K0164 antibody; mitochondrial antibody; ODPB_HUMAN antibody; pdhB antibody; PDHBD antibody; PDHE1 B antibody; PDHE1-B antibody; PHE1B antibody; Pyruvate dehydrogenase (lipoamide) beta antibody; Pyruvate dehydrogenase E1 beta polypeptide antibody; Pyruvate dehydrogenase E1 component subunit beta antibody; Pyruvate dehydrogenase E1 component subunit beta mitochondrial antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human PDHB

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

19E3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: U-251MG whole cell lysate(20µg),Hela whole cell lysate(20µg),HepG2 whole cell lysate(20µg),Jurkat whole cell lysate(20µg),HL-60 whole cell lysate(20µg),K562 whole cell lysate(20µg),U-937 whole cell lysate(20µg),Mouse brain tissue lysate(20µg),Rat brain tissue lysate(20µg)
All lanes: PDHB antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 39 kDa
Observed band size: 37 kDa
Exposure time：2min

Description

Pyruvate dehydrogenase E1 component subunit beta (PDHB) serves as an essential component of the pyruvate dehydrogenase complex, the critical metabolic gateway linking glycolysis to the citric acid cycle. This mitochondrial enzyme catalyzes the irreversible conversion of pyruvate to acetyl-CoA, positioning it at the center of cellular energy metabolism research. PDHB has gained particular attention in studies of metabolic reprogramming in cancer, mitochondrial disorders, and metabolic diseases where altered PDH complex activity plays a significant role.

This recombinant rabbit monoclonal antibody (clone 19E3) offers the reproducibility and consistency that demanding metabolic research requires. Generated against a synthetic peptide derived from human PDHB and produced through recombinant technology, this antibody delivers sequence-defined specificity with minimal lot-to-lot variation, ensuring your experimental results remain comparable across extended studies.

Validation testing demonstrates reliable performance across diverse sample types. Western blot analysis confirms detection in multiple human cell lines including U-251MG, HeLa, HepG2, Jurkat, HL-60, K562, and U-937, providing flexibility across different experimental models. The antibody also exhibits cross-species reactivity, with confirmed detection in mouse and rat brain tissue lysates, supporting translational research workflows. The observed band at 37 kDa runs slightly below the predicted 39 kDa molecular weight, a common occurrence attributable to post-translational processing or mitochondrial signal peptide cleavage during protein maturation.

Supplied in a stabilized liquid format with affinity-purified rabbit IgG, this antibody is ready for immediate use in western blotting at recommended dilutions of 1:500–1:2000 and ELISA applications. It represents a dependable tool for researchers investigating mitochondrial function, cellular metabolism, and energy homeostasis.

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Target Background

Function(From Uniprot)

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

Gene References into Functions

PDHB was downregulated in nasopharyngeal carcinoma cells. Forced expression of PDHB in NPC cells inhibited cell growth and migration, while knocking down the expression of PDHB promoted the growth, migration, and tumorigenesis of NPC cells. PDHB inhibited ERK signaling and cell growth driven by RasV12. PMID:26857147
Data indicate that (pro)renin receptor ATP6PA2 interacts with the E1 beta subunit of pyruvate dehydrogenase and controlling its protein stability PMID:25720494
PHD3 depletion did not affect the expression of the PDH-E1alpha, E1beta, and E2 subunits, or the phosphorylation status of E1alpha, but destabilized the PDH complex (PDC), resulting in less functional PDC. PMID:25088999
Sequencing of PDHB reveals a homozygous point mutation (c.302T>C), causing a predicted amino acid change in patient 1. Patient 2 is compound heterozygote for mutations c.301A>G (p.M101V) and c.313G>A (p.R105Q). PMID:19924563
Data indicate that PDH deficiency in our patient involves a post-translational modification in which EGFR-PTK-mediated tyrosine phosphorylation of the E1beta protein leads to enhanced ubiquitination followed by proteasome-mediated degradation. PMID:17923481
clinical findings are similar to those of PDHA1 deficiency, except that ataxia was more frequent in PDHA1 cases and consanguinity was found only in PDHB families. PMID:18164639
Species specificity in the interaction between hE1beta and hE2 in pyruvate dehydrogenase complex. PMID:18206651