PDHA1 Recombinant Monoclonal Antibody

Code: CSB-RA189996A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human, Rat

Application: ELISA, WB, IHC, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

P08559

Target Names

PDHA1

Alternative Names

ODPA_HUMAN antibody; PDH antibody; PDHA antibody; PDHA1 antibody; PDHCE1A antibody; PDHE1 A type I antibody; PDHE1-A type I antibody; PHE1A antibody; Pyruvate Dehydrogenase (lipoamide) alpha 1 antibody; Pyruvate dehydrogenase complex, E1 alpha polypeptide 1 antibody; Pyruvate Dehydrogenase E1 alpha antibody; Pyruvate dehydrogenase E1 component subunit alpha antibody; Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial antibody

Species Reactivity

Human, Rat

Immunogen

A synthesized peptide derived from human PDHA1

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5D9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: HepG2 whole cell lysate(20µg),COLO-205 whole cell lysate(20µg), A549 whole cell lysate(20µg), Hela whole cell lysate(20µg),HEK293T whole cell lysate(20µg),PC-3 whole cell lysate(20µg),Rat brain tissue lysate(20µg)
All lanes: PDHA1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 43 kDa
Observed band size: 45 kDa
Exposure time：1min
IHC image of CSB-RA189996A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing Hela cells stained with CSB-RA189996A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

Pyruvate dehydrogenase E1 component subunit alpha (PDHA1) serves as a critical gatekeeper in cellular energy metabolism, catalyzing the irreversible conversion of pyruvate to acetyl-CoA and linking glycolysis to the tricarboxylic acid cycle. This mitochondrial enzyme has emerged as a focal point in cancer metabolism research, where its regulation influences the Warburg effect, and in studies of metabolic disorders including pyruvate dehydrogenase deficiency. Understanding PDHA1 expression and localization provides valuable insights into cellular metabolic reprogramming across diverse physiological and pathological contexts.

This recombinant rabbit monoclonal antibody, clone 5D9, offers the reproducibility and consistency that demanding metabolic studies require. Generated against a synthetic peptide derived from human PDHA1 and produced through recombinant technology, this antibody delivers sequence-defined specificity with minimal lot-to-lot variation, ensuring your longitudinal studies maintain comparable results over time.

Validation across multiple platforms demonstrates robust performance for diverse experimental workflows. Western blot analysis detects PDHA1 at approximately 45 kDa across an extensive panel of human cell lines including HepG2, COLO-205, A549, HeLa, HEK293T, and PC-3, as well as rat brain tissue, confirming cross-species reactivity. The slightly higher observed molecular weight compared to the 43 kDa predicted size likely reflects post-translational modifications characteristic of this mitochondrial protein. Immunohistochemistry validation in human stomach tissue demonstrates clear staining suitable for tissue-based expression studies, while flow cytometry analysis in HeLa cells confirms utility for single-cell metabolic profiling applications.

Whether investigating metabolic reprogramming in cancer, mitochondrial dysfunction, or cellular bioenergetics, this antibody provides a reliable tool for characterizing PDHA1 across Western blot, immunohistochemistry, and flow cytometry platforms.

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Target Background

Function(From Uniprot)

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

Gene References into Functions

Case demonstrates fetal akinesia deformation sequence due to a de novo hemizygous c.498C > T splice-site mutation in the pyruvate dehydrogenase-alpha 1 (PDHA1) gene. PMID:28495245
Data show that metformin reduces hypoxia-inducible factor 1alpha (HIF-1alpha) gene expression and increases pyruvate dehydrogenase (PDH) expression. PMID:27474170
The report PDHA1 as a new component of brainstem-type Lewy bodies in idiopathic PD, DLB and PARK14, the level of PDHA1 protein being significantly decreased in the putamen and substantia nigra of patients with idiopathic Parkinson's disease . PMID:28564592
Findings show that serine-threonine-tyrosine kinase 1 (NOK) mediates glycolysis and nuclear pyruvate dehydrogenase complex (PDC) associated histone acetylation. PMID:28410146
The association of mitochondrial microphthalmia-associated transcription factor (MITF) with pyruvate dehydrogenase (PDH) emerges as an important regulator of mast cell function. Our findings indicate that PDH could arise as a new target for the manipulation of allergic diseases. PMID:27871875
our results show that negative PDHA1 gene expressionis associated with significantly higher cell stemness in prostate cancer cells and reduced protein expression of this gene is associated with shorter clinical outcome in prostate cancers. PMID:28076853
oncoprotein HBXIP enhances glucose metabolism reprogramming through suppressing SCO2 and PDHA1 in breast cancer PMID:26309161
Lack of PDHE1alpha protein expression is associated with Esophageal Squamous Cell Carcinoma. PMID:26408721
MPO and BPI in CD4(+)T-lymphocytes, and PDHA1 and MRPL42 in CD8(+) T-lymphocytes might be used as specific biomarkers of severe asthma progression. PMID:26107902
Phosphorylation at distinct serine and tyrosine residues inhibits PDHA1 through distinct mechanisms to impact active site accessibility. PMID:25104357
High HK2 expression combined with low phosphorylated PDHA1 expression in the invasive front lesions of colorectal tumors is predictive of tumor aggressiveness and survival. PMID:25060325
In the presence of PPARbeta/delta, Vpr induced a 3.3-fold increase in PPAR response element-driven transcriptional activity, a 1.9-fold increase in PDK4 protein expression, and a 1.6-fold increase in the phosphorylated pyruvate dehydrogenase subunit E1alpha. PMID:23842279
In this study the phenotypes of patients with PDH deficiency have been divided into three groups of mutations. PMID:19517265
Expression of the PDHA1 gene was found in all somatic cells, whereas expression of PDHA2 gene was restricted to germ cells. The switch from X-linked to autosomic gene expression occurred in spermatocytes. PMID:22750801
Molecular analysis of PDH1A revealed a novel hemizygous c.1045G>A mutation, predicting a p.A349T missense mutation. PMID:22142326
Skin fibroblast culture assay revealed PDH deficiency, confirmed by mutation analysis of the E1 alpha subunit. PMID:21895644
TNFalpha can inhibit pulmonary artery smooth muscle cells pyruvate dehydrogenase activity and induce a pulmonary arterial hypertension phenotype. PMID:21809123
We provide an efficient stepwise strategy for mutation screening in pyruvate dehydrogenase complex genes and expand the growing list of PDHA1 mutations analyzed at the structural level PMID:21914562
Data show that overexpression of ErbB2 maintains PDH flux by suppressing PDK4 expression in an Erk-dependent manner. PMID:21852536
4 affected female patients with PDHA1 mutations who had with severe cortical atrophy, dilated ventricles, and an incomplete corpus callosum. PMID:21723463
We document the broad variability of clinical symptoms of pdha1 deficiency disease. We proved that normal PDHc activity may not exclude the disease. PMID:21470495
Novel nonsense mutation (R263X) of the E1alpha subunit in pyruvate dehydrogenase complex deficiency PMID:20958858
PDHA1 mutations were screened in 40 patients with biochemically demonstrated pyruvate dehydrogenase complex deficiency deficiency or strong clinical suspicion and changes with probable pathological significance were found in 20. PMID:20002461
AAV3-mediated transfer and expression of the pyruvate dehydrogenase E1 alpha subunit gene causes metabolic remodeling and apoptosis of human liver cancer cells. PMID:19586787
model of the pyruvate dehydrogenase complex formed by E2 and E2 plus the E3-binding protein and binding of the E1 and E3 components PMID:14638692
determinant spreading might underlie the autoimmunity against Elalpha. PMID:14708897
PDHA1 gene mutations may have a role in Pyruvate dehydrogenase E1 subunit deficiency PMID:16713755
Molecular genetic analysis of the X-chromosomal E1alpha subunit of PDH showed three new mutations in phylogenetically conserved areas of the protein: Glu358Lys in patient 1; Arg88Lys in patient 2 and 3 (brothers); and Leu216Ser in patient 4. PMID:16967364
Short-term elevation in plasma non-esterified fatty acids at rest increases PDH-E1alpha phosphorylation, but exercise overrules this effect and phosphorylation leads to even dephosphorylation during exercise with intralipid infusion. PMID:17065338
Attenuated PDHa activity contributes to the preferential oxidation of n-6 PUFA during moderate-intensity exercise. PMID:17947500
Resting PDH protein content and phosphorylation on PDH-E1 alpha sites 1 and 2 were higher in vastus lateralis than in triceps and deltoid as was the activity of oxidative enzymes PMID:17957032
two synonymous mutations expand the spectrum of rare PDHA1 splicing mutations, all of which are located in non canonical splice sites. PMID:18023225
Our results encourage the use of amino acid supplementation to overcome the metabolic/biochemical changes induced by PDHA1 gene specific mutations associated with mild pyruvate dehydrogenase complex phenotypes. PMID:18398624