NRAS/HRAS/KRAS Recombinant Monoclonal Antibody

Code: CSB-RA048081A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, IHC, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

P01111 P01112 P01116

Target Names

NRAS/HRAS/KRAS

Alternative Names

NRAS; HRAS1; GTPase NRas; Transforming protein N-Ras; HRAS; HRAS1; GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; KRAS; KRAS2; RASK2; GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras

Species Reactivity

Human

Immunogen

A synthesized peptide from human NRAS/HRAS/KRAS protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2D11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA048081A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA048081A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing 786-O cells stained with CSB-RA048081A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Description

The RAS family of small GTPases—comprising NRAS, HRAS, and KRAS—represents one of the most intensively studied signaling nodes in cancer biology. These molecular switches cycle between active GTP-bound and inactive GDP-bound states to regulate cell proliferation, differentiation, and survival through the MAPK and PI3K pathways. Mutations in RAS genes occur in approximately 30% of all human cancers, with KRAS alterations particularly prevalent in pancreatic, colorectal, and lung adenocarcinomas. Understanding RAS expression patterns and localization remains essential for researchers investigating oncogenic signaling mechanisms and therapeutic resistance.

This recombinant monoclonal antibody (clone 2D11) targets a conserved epitope shared across NRAS, HRAS, and KRAS, enabling simultaneous detection of all three family members in a single experiment. The recombinant production method ensures that every lot delivers identical binding characteristics, eliminating the variability that can compromise longitudinal studies or multi-site collaborations. As a sequence-defined reagent, this antibody provides the reproducibility that rigorous experimental workflows demand.

Validation studies demonstrate reliable performance across multiple platforms. Immunohistochemistry staining at 1:100 dilution reveals clear signal in paraffin-embedded human small intestine and liver cancer tissues using citrate buffer antigen retrieval, offering researchers a validated approach for examining RAS expression in both normal and malignant contexts. Flow cytometry analysis of 786-O renal carcinoma cells confirms intracellular detection capability, with distinct positive shifts compared to isotype controls at 1:100 dilution following formaldehyde fixation and Triton X-100 permeabilization.

Whether you are mapping RAS expression across tumor microenvironments or characterizing signaling pathway activation in cell-based models, this antibody provides the consistency and flexibility needed for signal transduction research.