NPPB Recombinant Monoclonal Antibody

Code: CSB-RA016021MA1HU

Rate （N/A）
Review （0）

Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, FC

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

P16860

Target Names

NPPB

Alternative Names

ANFB_HUMAN antibody; BNP antibody; BNP(1-32) antibody; BNP(5-29) antibody; BNP-32 antibody; Brain type natriuretic peptide antibody; Gamma brain natriuretic peptide antibody; Gamma-brain natriuretic peptide antibody; Natriuretic peptide precursor B antibody; nppb antibody; NT proBNP antibody

Species Reactivity

Human

Immunogen

Recombinant Human NPPB protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

5D7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Overlay Peak curve showing 786-O cells stained with CSB-RA016021MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

Natriuretic peptide precursor B, commonly known as BNP or brain natriuretic peptide, serves as a critical biomarker in cardiovascular research and clinical diagnostics. Produced primarily by ventricular cardiomyocytes in response to mechanical stretch and neurohormonal activation, NPPB and its cleavage products—including the widely measured NT-proBNP fragment—provide valuable insights into cardiac function, heart failure progression, and hemodynamic stress. Understanding NPPB expression patterns and regulation remains essential for researchers investigating cardiac pathophysiology, biomarker development, and therapeutic interventions targeting the natriuretic peptide system.

This recombinant monoclonal antibody, clone 5D7, offers the reproducibility and consistency that demanding cardiovascular research requires. Generated against recombinant human NPPB protein and expressed with a human IgG1 framework, this antibody provides sequence-defined specificity that eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived reagents. Affinity chromatography purification ensures high purity suitable for sensitive detection methods.

Validation studies demonstrate reliable performance in flow cytometry applications, with testing conducted on 786-O cells using intracellular staining protocols. The recommended dilution range of 1:50 to 1:200 for flow cytometry provides flexibility for optimization across different experimental conditions. The antibody has also been validated for ELISA applications, supporting quantitative detection workflows.

Supplied in a glycerol-based PBS buffer at pH 7.4 with Proclin 300 preservative, this unconjugated format allows researchers to select secondary detection systems best suited to their specific instrumentation and experimental design. This antibody supports investigations into cardiac biomarker biology, heart failure mechanisms, and natriuretic peptide signaling pathways where consistent, reliable detection of human NPPB is essential.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Binds the clearance receptor NPR3.; May affect cardio-renal homeostasis. Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32.; May have a role in cardio-renal homeostasis. Able to promote the production of cGMP.

Gene References into Functions

NT-BNP is elevated in fetal aortic stenosis. PMID:29327361
Combinative index (arterial lactate, NT-proBNP and CRP) could serve as a valuable predictor for the 28-day mortality rate in patients with sepsis. PMID:30158031
Patients diagnosed with portopulmonary hypertension (POPH) had significantly higher BNP levels in comparison with those patients without. In contrast, PELD/MELD scores showed an almost negligible correlation with the BNP level. Liver transplantation was successful in 3 asymptomatic biliary atresia patients with POPH who had high BNP levels despite the low PELD/MELD scores. PMID:29733490
NT-proBNP was associated with poor outcomes for patients with acute respiratory distress syndrome, and its level predicted mortality. PMID:28322314
Levels of N-terminal pro-brain-type natriuretic peptide increases in response to exercise in patients with pulmonary arterial hypertension. PMID:29334941
Admission NT-proBNP is an independent predictor for short- and long-term adverse events in Takotsubo syndrome patients. PMID:29675571
Report a weak correlation between B-type natriuretic peptide and within-visit blood pressure variability. PMID:29604077
Lung ultrasound, serum B-type natriuretic peptide levels, and echocardiography can be used for outcome prediction in patients with acute heart failure. PMID:29532155
Data suggest that intact FGF23 level in plasma is independent predictor of cardiovascular death in patients with heart failure and provides added value to standard of care, natriuretic peptide (NT-proBNP) plasma level, for risk estimation. This study was conducted in Belgium. (FGF23 = fibroblast growth factor 23; NT-proBNP = aminoterminal pro-B-type natriuretic peptide) PMID:30205090
Report decreased NT-proBNP levels in heart failure patients in contemporary heart failure management programs. PMID:29656687
NT-proBNP may have a role in myocardial conduction abnormalities in patients with diabetes mellitus PMID:29121498
recombinant human brain natriuretic peptide can regulate expression of IL-6, TNF-alpha, and IL-10 in lipopolysaccharide-activated RAW 264.7 cells. PMID:29754152
n elderly patients with symptoms of heart failure ST2 was not superior to NTproBNP to predict all cause or cardiovascular mortality. Furthermore, it is unclear if the combination of ST2 and NTproBNP will improve long-term prognostication beyond what is achieved by NTproBNP alone. PMID:29355441
current cutoffs for diagnosing HF for NT-proBNP and BNP have relatively low diagnostic concordance and correlation, particularly among patients with chronic kidney disease PMID:30111510
Elevated plasma NT-proBNP and KIM-1 in patients with stage 5 chronic kidney disease (CKD5) undergoing maintenance hemodialysis (HD) and HF (+) (heart failure)CKD5-HD patients suggest that natriuretic peptides and KIM-1 may contribute to the pathogenesis of HF in CKD5-HD patients. PMID:28990414
Increased levels of NT-proBNP seems to be a better biomarker of multidrug antihypertensive therapy requirement than CT-proAVP. PMID:29190613
Baseline NT-proBNP concentration was associated with short-term stroke among patients hospitalized with acute heart filure. Stroke risk assessment models should consider incorporation of NT-proBNP measurement. PMID:28905172
Suggest potential role for BNP as a protective endogenous factor against cerebral ischemia. PMID:29203579
In children with chronic kidney disease, BNP is an indicator of heart failure correlating with renal function parameters and left ventricular mass index. PMID:29775443
Data indicate that B-type natriuretic peptide (BNP) was outcome associated in acute heart failure patients. PMID:28132515
Combination of coronary artery calcium score and NT-proBNP could be used to identify a group of coronary artery disease patients where MPI could be averted with a high degree of diagnostic safety. PMID:27005324
NT-pro-BNP was not significantly different between subjects with 1-2 stenotic coronary arteries compared to those with 3 stenotic coronary arteries. PMID:28845719
Serum levels of ST2, IL-33 and BNP were independent risk factors for major adverse cardiovascular events in acute myocardial infarction after percutaneous coronary intervention. PMID:28623858
Circulating NT-proBNP levels are lower in overweight patients, even in those with diabetes, compared to controls. PMID:28946871
Report usefulness of point-of-care test for NT-proBNP in primary care for the diagnosis of heart failure. PMID:29263170
In-hospital mortality in patients with sepsis and low risk of sepsis-related organ failure was associated with NT-proBNP > 1,330 pg/ml. PMID:28473224
The results of the present study suggest the possible use of the molecular pathology of BNP for the determination of the terminal cardiac function in SCD and analysis of its death mechanism in forensic practice. PMID:28765973
In patients with a recent acute coronary syndrome and type 2 diabetes mellitus, BNP and NT-proBNP were powerful predictors of cardiovascular outcomes beyond heart failure and death, ie, were also predictive of MI and stroke. PMID:28554908
Data suggest that subjects with morbid obesity exhibit down-regulation of serum levels of BNP compared to control subjects. This study was conducted in Italy. PMID:27426417
Increased baseline values of BNP (and BigET-1) after acute myocardial infarction, were independently predictive of future cardiovascular events. PMID:28284500
Patients with acute exacerbation of COPD and elevated NT-pro-BNP levels had increased hospital length of stay and need for intensive care. PMID:28458528
BNP blood level is a biomarker for the the severity of hand-foot-mouth disease PMID:28962547
Plasma levels of NT-pro-BNP were significantly higher in hypertrophic cardiomyopathy (HCM) patients with more advanced disease. Elevated NT-pro-BNP not only reflects the diastolic impairment of the LV, but it might also be the result of cardiac ischemia in patients with HCM. PMID:28336204
Increased preoperative brain natriuretic peptide concentration was associated with risk of serious adverse events in pediatric cardiac surgery patients. PMID:27919715
NPPB SNPs were not associated with insulin resistance in older adults. PMID:27101535
Higher blood levels of NT-proBNP was associated with incident cognitive impairments. PMID:27567834
In people with Type 2 diabetes, high baseline B-type natriuretic peptide levels were significantly associated with future atrial fibrillation development. PMID:26173591
Plasma BNP levels were independently associated with functional outcomes 3 months after stroke onset in atrial fibrillation patients. PMID:28253498
Short-term follow-up BNP and percent change in BNP level are significant prognostic factors of all-cause mortality in hospitalized heart failure patients. PMID:28774268
NT-proBNP showed an incremental prognostic value for prediction of adverse outcomes when added to the clinical and echocardiographic parameters in patients with left ventricular dysfunction. PMID:28349264
BNP gene body hypermethylation is associated with the risk of rheumatic heart disease (RHD), and also influenced by the warfarin anticoagulant therapy of RHD patients after surgery, which could represent novel and promising targets for therapeutic development. PMID:27920275
IL-6 receptor blockade has an effect on high-sensitivity troponin T and NT-proBNP in rheumatoid arthritis PMID:27744141
An exercise-associated increase in hs-cTnT and NT-proBNP occurred in response to a 60-minute maximal swimming test that was independent of pubertal status/adolescent vs adults. The present data also suggests that baseline and postexercise hs-cTnT values are higher in male compared with female, with no sex differences in NT-proBNP values. PMID:29173327
An increase in NT-proBNP is a strong predictor of outcome in patients with left ventricular non-compaction cardiomyopathy. PMID:28268080
Brain Natriuretic Peptide levels increased as heart failure duration increased. PMID:28268084
In this hospitalized heart failure cohort, atrial fibrillation/flutter status did not influence post-discharge NT-proBNP trajectory. PMID:27748006
NT-proBNP and BNP levels were statistically higher in patients with heart failure and anemia compared to patients with heart failure and without anemia. PMID:28587950
The rs198389 G allele in the NPPB promoter is associated with elevated levels of NT-proBNP throughout adult life, reduced blood pressure, hypertension and cardiovascular mortality, and increased lifespan. PMID:28341776
Atrial fibrillation risk discrimination was not significantly improved by the addition of NT-proBNP levels to the Framingham AF risk model. PMID:27194537
Combination of high serum BNP levels, along with high soluble AXL had greater predictive value for heart failure than BNP alone. PMID:27718443