NONO Recombinant Monoclonal Antibody

Code: CSB-RA273277A0HU

Rate （N/A）
Review （0）

Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, WB, IHC, IF

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

Q15233

Target Names

NONO

Alternative Names

Non-POU domain-containing octamer-binding protein (NonO protein) (54 kDa nuclear RNA- and DNA-binding protein) (55 kDa nuclear protein) (DNA-binding p52/p100 complex, 52 kDa subunit) (NMT55) (p54(nrb)) (p54nrb), NONO, NRB54

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human NONO / p54nrb

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8C11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, Ntera-2 whole cell lysate
All lanes: NONO Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55, 44 kDa
Observed band size: 55 kDa
IHC image of CSB-RA273277A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA273277A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of Hela Cells with CSB-RA273277A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Description

NONO, also known as p54nrb, is a multifunctional nuclear protein that plays essential roles in RNA processing, transcription regulation, and DNA repair. As a component of the paraspeckle nuclear bodies, NONO participates in the retention of hyperedited RNAs in the nucleus and contributes to circadian clock regulation. Its involvement in diverse nuclear processes makes it a valuable target for researchers investigating epigenetics, nuclear signaling, and gene expression mechanisms.

This recombinant monoclonal antibody, generated from clone 8C11, offers the consistency and reproducibility that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly in rabbit host cells, researchers benefit from lot-to-lot uniformity that traditional hybridoma-derived antibodies cannot guarantee. This reliability proves particularly valuable for longitudinal studies or when standardizing protocols across research groups.

Validation data demonstrates robust performance across multiple applications. In western blot experiments, the antibody detects NONO at the expected 55 kDa molecular weight across diverse human cell lines including 293, HeLa, HepG2, and Ntera-2, confirming consistent target recognition regardless of cellular context. Immunohistochemistry studies reveal clear nuclear staining in paraffin-embedded human breast cancer and brain tissue sections, supporting its utility in pathological investigations. For subcellular localization studies, immunofluorescence analysis in HeLa cells shows distinct nuclear signal that correlates well with DAPI counterstaining, enabling precise visualization of NONO distribution.

The unconjugated format and affinity-purified preparation provide flexibility for researchers to pair this antibody with their preferred detection systems. Whether investigating paraspeckle biology, exploring NONO's role in cancer progression, or studying nuclear RNA processing pathways, this antibody delivers the specificity and versatility needed for rigorous experimental investigation.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. Together with PSPC1, required for the formation of nuclear paraspeckles. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Important for the functional organization of GABAergic synapses. Plays a specific and important role in the regulation of synaptic RNAs and GPHN/gephyrin scaffold structure, through the regulation of GABRA2 transcript. Plays a key role during neuronal differentiation by recruiting TET1 to genomic loci and thereby regulating 5-hydroxymethylcytosine levels. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.

Gene References into Functions

Results show that NONO is upregulated in human esophageal squamous cell carcinoma (ESCC) tissue samples. Other findings suggest that NONO plays a potent role in multiple biological aspects of ESCC through activation of the Akt and Erk1/2 signaling pathways. PMID:29620226
we observed, for the first time, that the expression of p54 was markedly increased in the synovial tissues of rheumatoid arthritis patients. PMID:29441870
Findings reveal a new mechanism whereby HUR protects NONO from mir320-mediated degradation upon UVC exposure and identify a new component within the complex network of players underlying the DNA damage response adding mir320a to the list of p53-regulated targets upon genotoxic stress. PMID:27816966
GAPLINC has a role in promoting colorectal cancer invasion via binding to PSF/NONO and partly by stimulating the expression of SNAI2 PMID:27259250
Here, it is reported that l-proline stabilizes purified NONO homodimers, enabling good-quality solution small-angle X-ray structure determination and crystallization. NONO crystallized in the apparent space group P21 with a unique axis (b) of 408.9 A and with evidence of twinning, as indicated by the cumulative intensity distribution L statistic, suggesting the possibility of space group P1. PMID:27303796
Mechanistic dissection reveals that NEAT1 broadly interacts with the NONO-PSF heterodimer as well as many other RNA-binding proteins and that multiple RNA segments in NEAT1, including a 'pseudo pri-miRNA' near its 3' end, help attract the Drosha-DGCR8 Microprocessor. PMID:28846091
The SFPQ*NONO complex contains an RNA binding domain, and prior work has demonstrated diverse roles in RNA metabolism. It is thus plausible that the additional repair function of NONO, revealed in cell-based assays, could involve RNA interaction. PMID:27924002
Exome analysis identified a novel de novo splice-site variant c.1171+1G>T in exon 11 of NONO gene in a patient with intellectual disability and non-compaction cardiomyopathy. PMID:27329731
Case Report: melanotic PEComa of the sinonasal mucosa with NONO-TFE3 fusion. PMID:28009605
High expression of P54 is associated with breast cancer. PMID:27297086
The present study indicates that p54nrb is a powerful molecule involved in the regulation of cell motility and promotes the migration and invasion of THP1 cells, and it is more likely to be involved in the release of inflammatory mediators and the motility of inflammatory cells. PMID:27108701
p54(nrb) is a novel regulator of SREBP-1a in the nucleus, and the data suggest that p54(nrb) regulation of SREBP-1a supports the increased cellular demand of lipids for breast cancer growth. PMID:26148231
Data uncover a new role for NONO in mediating the cellular response to UV-induced DNA damage. PMID:25893301
Subnuclear re-localization of SOX10 and p54NRB correlates with a unique neurological phenotype associated with SOX10 missense mutations PMID:26060192
These data suggest that NonO negatively regulates HIV-1 infection in CD4(+) T cells, highlighting the importance of host proteins associated with HIV-1 preintegration complexes in regulating viral replication. PMID:25769457
Mutations in NONO led to defects at inhibitory synapses in intellectual disability syndrome. PMID:26571461
suggest that SFPQ.NONO promotes end joining by binding to internal DNA sequences and cooperating with other repair proteins to stabilize a synaptic pre-ligation complex PMID:25998385
Patients with aortic dissection (AD)exhibited significantly decreased expression of P54(nrb) /NonO. The significant correlation between P54(nrb) /NonO and collagen may point to novel thinking about collagen metabolism research in AD aorta. PMID:24720418
silencing p54(nrb)/NONO expression in H295R human adrenocortical cells decreases the ability of the cells to increase intracellular cAMP production and subsequent cortisol biosynthesis in response to adrenocorticotropin hormone (ACTH) stimulation. PMID:25605330
The ability of the SFPQ/NONO complex to form varying protein assemblies, in conjunction with the effect of post-translational modifications of SFPQ modulating mRNA binding, suggests key roles affecting mRNP dynamics within the cell. PMID:25605962
p54 is essential for GC-mediated expression of occludin, claudin-5, and barrier induction, and the p54/PSF heterodimer may contribute to normal blood-retinal barrier (BRB) induction in vivo. PMID:23640037
Localisation of TopBP1 at DNA damage sites was noticed as early as 5 s following damage induction, whereas p54(nrb) and PSF localised there after 20 s. PMID:22213094
The crystal structure of the heterodimer of the multidomain conserved region of the Drosophila behavior/human splicing proteins, PSPC1 and NONO, is described. PMID:22416126
Study showed that Rasd1 and NonO interact at the CRE-site of specific target genes. PMID:21915321
crystal of PSPC1-NONO contained one heterodimer in the asymmetric unit and diffracted to 1.9 A resolution using synchrotron radiation PMID:22102035
p54(nrb) interaction with RNA could be selectively modulated by phosphorylation during mitosis PMID:21819346
RVxF motifs play an important role in controlling the multifunctional properties of p54nrb and PSF in the regulation of gene transcription PMID:21566083
High p54nrb is associated with malignant melanoma. PMID:21642354
NONO/SFPQ heterodimer is involved in the early stage of the DSB response. PMID:20421735
The nmt55/p54nrb protein is post-transcriptionally regulated in human breast tumors leading to reduced expression in ER- tumors and the expression of an amino terminal altered isoform in a subset of ER+ tumors. PMID:11710964
PSF and p54nrb bind U5 snRNA with both the sequence and structure of stem 1b contributing to binding specificity.PSF interacts with p54nrb PMID:12403470
Review article of p54 as a multi-functional nuclear protein. PMID:12417296
nmt55 expression is related to hormone-dependency or -independence associated with the androgen receptor PMID:12672026
Results suggest that p54(nrb) functions as a coactivator of androgen receptors that potentiates transcription and possibly splicing. PMID:12810069
p54nrb and PSF have properties of key factors mediating INS function and likely define a novel mRNA regulatory pathway that is hijacked by HIV-1. PMID:12944487
p54(nrb), which binds the C-terminal domain of the largest subunit of RNAPII, can interact directly with the 5' SS indicates that these factors may mediate contacts between RNA polymerase II PMID:15057275
The PSF.p54(nrb) complex cooperates with Ku protein to form a functional preligation complex with substrate DNA PMID:15590677
Thus paraspeckles are the sites where a subset of the total cellular pool of p54nrb is targeted in a RNA Polymerase II-dependent manner. PMID:16148043
These data demonstrated that PSF and p54nrb complex with AR and play a key role in modulating AR-mediated gene transcription. PMID:17452459
Identification of PSF, p54(nrb), PTB, and U1A as proteins specifically bound to the COX-2 polyadenylation signal upstream sequence elements . PMID:17507659
p54 is present along the length of genes, and small interfering RNA (siRNA)-mediated knockdown leads to defects in XRN2 recruitment and termination. PMID:17639083
Findings suggest that the PSF.p54nrb complex is a novel MAP kinase signal-integrating kinases substrate that binds the mRNA for tumor necrosis factor alpha. PMID:17965020
at the TERT gene locus in human tumour cells containing a functional SWI/SNF complex, Brm, and possibly BRG1, in concert with p54(nrb), would initiate efficient transcription and could be involved in the subsequent splicing of TERT transcripts PMID:18042045
TORC2 and NONO complex on cAMP-responsive promoters, and NONO acts as a bridge between the CREB/TORC complex and RNA polymerase II. PMID:18077367
Co-expression with polypyrimidine tract-binding protein-associated splicing factor (PSF) relocates oncogenic RING finger protein 43 (RNF43) from the nuclear periphery to the nucleoplasm. PMID:18655028
p54nrb plays an important role in the regulation of Sox9 function and the formation of paraspeckle bodies during chondrogenesis PMID:18677406
SOCS3 regulates the action of NonO to suppress MUC8 transcriptional activation. PMID:18952062
p54nrb is a transcriptional corepressor of the progesterone receptor that modulates transcription of the labor-associated gene, connexin 43 (Gja1) PMID:19423654
confirm the interactions of eEF1A1, p54(nrb), hnRNP-L, GAPDH and ASF/SF2 with the right terminal stem-loop domain of HDV genomic RNA in vitro PMID:19464723
NONO is a TORC-interacting protein that is necessary for cAMP-dependent activation of CREB target genes in vivo. TORC2 and NONO complex on cAMP-responsive promoters, and NONO acts as a bridge between the CREB/TORC complex and RNA polymerase II. PMID:18077367

Hide All

Subcellular Location

Nucleus. Nucleus, nucleolus. Nucleus speckle. Chromosome. Note=Detected in punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles.

Tissue Specificity

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.

Database Links

HGNC: 7871

UNIGENE: Hs.533282

KEGG: hsa:4841

STRING: 9606.ENSP00000276079

OMIM: 300084

Promotions