NFKBIA Recombinant Monoclonal Antibody

Code: CSB-RA015761A0HU

Rate （5.0）
Review （1）
Citation （1）

Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, WB, IHC, IP

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Product Details
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Target Background

Product Details

Uniprot NO.

P25963

Target Names

NFKBIA

Alternative Names

I kappa B alpha antibody; I-kappa-B-alpha antibody; IkappaBalpha antibody; IkB-alpha antibody; IKBA antibody; IKBA_HUMAN antibody; IKBalpha antibody; MAD 3 antibody; MAD3 antibody; Major histocompatibility complex enhancer-binding protein MAD3 antibody; NF kappa B inhibitor alpha antibody; NF-kappa-B inhibitor alpha antibody; NFKBI antibody; NFKBIA antibody; Nuclear factor of kappa light chain gene enhancer in B cells antibody; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human NFKBIA

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1E9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunoprecipitation (IP) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IP	1:200-1:1000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, PC3 whole cell lysate, THP-1 whole cell lysate
All lanes: NFKBIA antibody at 0.79μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
IHC image of CSB-RA015761A0HU diluted at 1:79.75 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating NFKBIA in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA015761A0HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA015761A0HU (3μg) + HepG2 whole cell lysate (500μg)
Lane 3: HepG2 whole cell lysate (20μg)

Description

NFKBIA, also known as IκB-alpha, serves as a critical regulator of the NF-κB signaling pathway by sequestering NF-κB transcription factors in the cytoplasm and preventing their nuclear translocation. This inhibitory function makes NFKBIA a central focus in research exploring inflammatory responses, immune regulation, and cancer biology, where dysregulated NF-κB signaling frequently contributes to disease progression.

This recombinant monoclonal antibody, developed using rabbit host cells and clone 1E9, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs through recombinant expression, researchers benefit from lot-to-lot uniformity that traditional hybridoma-derived antibodies cannot guarantee. This reliability proves especially valuable for longitudinal studies or when establishing standardized protocols across research teams.

Validation testing demonstrates robust performance across multiple applications. Western blot analysis confirms specific detection of NFKBIA at the expected 36 kDa molecular weight across a diverse panel of human cell lines, including HeLa, HepG2, Jurkat, Raji, PC3, and THP-1 lysates, providing confidence when working with various cellular models. The antibody has also been validated for immunohistochemistry in paraffin-embedded human endometrial cancer tissue, enabling researchers to examine NFKBIA expression patterns within intact tissue architecture. Additionally, immunoprecipitation studies in HepG2 lysates confirm the antibody's utility for protein interaction studies and enrichment applications.

The affinity-purified formulation arrives ready for use across ELISA, western blotting, immunohistochemistry, and immunoprecipitation workflows. For investigators studying signal transduction mechanisms, inflammatory pathways, or NF-κB-dependent processes in cancer and immunity, this antibody provides a dependable tool for characterizing NFKBIA expression and regulation.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

Submit a Review here

Applications : Western blotting

Sample type: cells

Review: The protein (total p65/IκB-α and phosphorylated p65/IκB-α) levels were measured via western blot assay after myrislignan treatment in U87 and U251 cells.

By Anonymous

Citations

NF-κB Inhibitor Myrislignan Induces Ferroptosis of Glioblastoma Cells via Regulating Epithelial-Mesenchymal Transformation in a Slug-Dependent Manner Y Zhou,/,2023

Target Background

Function(From Uniprot)

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Gene References into Functions

this study shows changes of NF-kB expression levels induced by cell-free DNA in different cell types PMID:29743966
the results of real-time PCR and western blotting revealed that Huaier extract decreased p65 and c-Met expression and increased IkappaBalpha expression, while paclitaxel increased p65 expression and reduced IkappaBalpha and c-Met expression.The molecular mechanisms may be involved in the inhibition of the NF-kappaB pathway and c-Met expression PMID:29039556
Colorectal cancer cases showed an increase in the frequency of NFkappaBIA-881G allele in Egyptian subjects. PMID:28389768
association between polymorphisms and progression of chronic hepatitis B Virus infection among Chinese Han Population PMID:29093318
miR-668 was upregulated in radioresistant human breast cancer cell lines MCF-7R and T-47DR, which targeted IkappaBalpha, activated the NF-kappaB pathway and thus increased the radioresistance of breast cancer cells. PMID:28138801
We showed that pristimerin suppressed tumor necrosis factor a (TNFalpha)-induced IkappaBa phosphorylation, translocation of p65, and expression of NFkappaB-dependent genes. Moreover, pristimerin decreased cell viability and clonogenic ability of Uveal melanoma (UM)cells. A synergistic effect was observed in the treatment of pristimerin combined with vinblastine, a frontline therapeutic agent, in UM. PMID:28766683
Data collectively demonstrate the functional importance of IkappaBalpha-mediated stripping of NFkappaB from DNA in the kinetic control of NFkappaB signaling. PMID:28167786
These findings indicate that genetic polymorphisms of NFKB1A rs696, pre-miR-146a rs2910164 and pre-miR-499 rs3746444 may represent novel markers of AT susceptibility. PMID:28674224
XPO1 inhibitor combination therapy with bortezomib or carfilzomib induces nuclear localization of IkappaBalpha and overcomes acquired proteasome inhibitor resistance in human multiple myeloma. PMID:27806331
Molecular docking analysis indicated that transcription factor NF-kappaB was one of the potential molecular targets modulated by DTTF. Specifically, the drug blocked the TNFalpha-induced phosphorylation of upstream IkappaBalpha kinase in a time-dependent manner leading to the suppression of NF-kappaB activation and nuclear translocation PMID:27882436
We show that HOTAIR regulates activation of NF-kB by decreasing Ik-Ba (NF-kB inhibitor) and establish that by inducing prolonged NF-kB activation and expression of NF-kB target genes during DNA damage, HOTAIR has a critical role in cellular senescence and platinum sensitivity PMID:27041570
Here, the authors report amide hydrogen/deuterium exchange data that reveal long-range allosteric changes in the NFkappaB (RelA-p50) heterodimer induced by DNA or IkappaBalpha binding. PMID:28249778
Sam68 is essential for DNA damage-induced NF-kappaB activation and colon tumorigenesis. PMID:27458801
Specifically, BCA2 serves as an E3 SUMO ligase in the SUMOylation of IkappaBalpha, which in turn enhances the sequestration of NF-kappaB components in the cytoplasm. Since HIV-1 utilizes NF-kappaB to promote proviral transcription, the BCA2-mediated inhibition of NF-kappaB significantly decreases the transcriptional activity of HIV-1. PMID:28122985
This study found that NFKBIA mRNAs was significantly expressed in normal tissues compared with glioma specimens. PMID:27538656
Findings indicate the prognostic value of NFKB inhibitor alpha (NFKBIA) in lower-grade gliomas (LGGs). PMID:27052952
W346 effectively inhibited tumor necrosis factor (TNF-a)-induced NF-kappaB activation by suppressing IKK phosphorylation, inhibiting IkB-a degradation, and restraining the accumulation of NF-kappaB subunit p65 nuclear translocation. W346 also affected NF-kappaB-regulated downstream products involved in cycle arrest and apoptosis. PMID:26520440
treating cells with SZC014 resulted in a decrease in phosphorylation of IkBa and NF-kappaB/p65 and NF-kappaB/p65 nuclear translocation. The cytotoxic activities of seven OA derivatives were generally stronger than that of OA, among which, SZC014 possessed the most potent anticancer activity in SGC7901 cells and would be a promising chemotherapic agent for the treatment of gastric cancer PMID:26547583
Network analysis identified NFKBIA as pathogenic gene in childhood asthma. PMID:27420950
HMBA was able to increase prostratin-induced phosphorylation and degradation of NF-kappaB inhibitor IkappaBalpha, thereby enhancing and prolonging prostratin-induced nuclear translocation of NF-kappaB, a prerequisite for stimulation of transcription initiation PMID:27529070
enhanced miR-381a-3p expression contributed to the injury of osteoarthritis mainly by inhibiting the expression of IkappaBalpha. PMID:27312547
Timely and efficient degradation of ubiquitinated IkappaB[alpha], concomitant with timely and efficient liberation of RelA from ubiquitinated IkappaB[alpha] and RelA nuclear translocation, essentially depends on the presence of functional p97/VCP. PMID:26463447
Activated Rac1 regulates the degradation of IkappaBalpha and the nuclear translocation of STAT3-NFkappaB complexes in starved cancer cells PMID:27151455
mutation in Chinese patient results in mycobacterial infections without ectodermal dysplasia PMID:26691317
DAT stabilized IkBa by inhibiting the phosphorylation of Ika by the IkB kinase (IKK) complex. DAT induced proteasomal degradation of TRAF6, and DAT suppressed IKKb-phosphorylation through downregulation of TRAF6 PMID:26647777
the rs3138053 polymorphism of NFKBIA gene is a candidate for susceptibility to overall cancers, while rs696 plays a protective role [meta-analysis] PMID:26488500
Identify a novel BCR-ABL/IkappaBalpha/p53 network, whereby BCR-ABL functionally inactivates a key tumor suppressor in chronic myeloid leukemia. PMID:26295305
Study demonstrates an association between functional polymorphisms of IkappaBalpha rs696 and smoking with the risk of defective spermatogenesis, suggesting some interaction between the NF-kappaB signalling pathway and smoking-related ROS in human spermatogenesis. PMID:25352423
genetic variation associated with susceptibility to acute kidney injury PMID:26477820
MicroRNA-19a mediates gastric carcinoma cell proliferation through the activation of IkappaBalpha. PMID:26239140
No association was observed between NFKBIA variants and risk of liver cancer. PMID:24578542
SM22alpha is a phosphorylation-regulated suppressor of IKK-IkappaBalpha-NF-kappaB signaling cascades. PMID:25937534
Data suggest that activity of IKBalpha can be regulated by dietary factors; dietary supplementation with luteolin inhibits vascular endothelial inflammation by suppressing IKBalpha/NFkappaB signaling. PMID:25577468
This review and meta-analysis of the association of NFKBIA -881 A>G polymorphism with cancer susceptibility reveals that -881 A>G polymorphism may increase risk of cancer development in Asian populations. PMID:26252270
miR-126 may play an important role in hepatic fibrosis by downregulating the expression of IkappaBalpha partly through the NF-kappaB signaling pathway. PMID:25974152
IkappaBetaalpha inhibits apoptosis at the outer mitochondrial membrane independently of NF-kappaB retention. PMID:25361605
The single nucleotide polymorphism rs1957106 CT and TT genotypes were found to be associated with lower NFKBIA protein levels and a poor prognosis of pateints with glioblastoma. PMID:25215581
data suggest that the NFKBIA 126 G/A polymorphism might be potentially helpful to identify liver transplant recipients with an increased susceptibility to develop recurrent acute rejections PMID:25112903
Expression of IkappaBalpha in human bladder cancer cells is negatively correlated with epithelial-mesenchymal transition and tumor invasion in vitro. PMID:25374080
NFKBIA-rs2233419AG was associated with a significantly increased risk of developing recurrent wheezing. PMID:25326706
miR-196a can directly interact with IkappaBalpha 3'-UTR to suppress IkappaBalpha expression and subsequently promote activation of NF-kappaB PMID:24463357
MiR-196a promotes pancreatic cancer progression by targeting nuclear factor kappa-B-inhibitor alpha. PMID:24504166
Data indicate that following bortezomib treatment there was accumulation of IkappaB-alpha (IkappaBalpha) without affecting its phosphorylation status at an early time point. PMID:23697845
This study reveals that polymorphisms in the IkB-alpha promoter (-881 A/G, -826 C/T) are strongly associated with the susceptibility of Iranian Multiple Sclerosis patients PMID:24368589
The results of this study suggested that oligodendroglial IkappaBalpha expression and NF-kappaB are activated early in the course of MSA and their balance contributes to the decision of cellular demise. PMID:24361600
No statistically significant CRC risk association was found for the NFKBIA polymorphisms. PMID:23996241
data indicate that NFKBIA deletions are present but not frequent in Glioblastoma multiforme (GBM); the deletions become frequent in GBM neurospheres (NS), an event that seems to be affected by the presence of EGF in the culture medium PMID:24330732
the analysis of IkappaBalpha expression at salivary gland epithelial cell level could be a potential new hallmark of Sjogren's syndrome progression. PMID:23377923
IkappaBalpha sequesters not only p65 but also RPS3 in the cytoplasm. PMID:24457201
NF-kB expression was downregulated and its cytoplasmatic inhibitor IKBalpha was increased in CTLA4-Ig treated macrophages. PMID:24295474