NEUROD1 Recombinant Monoclonal Antibody

Code: CSB-RA982003A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, IF, FC

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Target Background

Product Details

Uniprot NO.

Q13562

Target Names

NEUROD1

Alternative Names

Neurogenic differentiation factor 1 (NeuroD) (NeuroD1) (Class A basic helix-loop-helix protein 3) (bHLHa3), NEUROD1, BHLHA3 NEUROD

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human NEUROD1

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5D2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Immunofluorescence staining of A549 with CSB-RA982003A0HU at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 522-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA982003A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

NEUROD1 is a basic helix-loop-helix transcription factor that plays a central role in neuronal differentiation and the development of pancreatic endocrine cells. Its involvement in neurogenesis, glucose homeostasis, and cell fate determination makes it a compelling target across neuroscience, metabolism, and cancer research, particularly in studies of neuroendocrine tumors and beta cell biology.

This recombinant monoclonal antibody, clone 5D2, offers the reproducibility and sequence-defined consistency that demanding experimental workflows require. Because the antibody sequence is fixed and produced recombinantly, researchers can expect uniform performance across experiments and over time, eliminating the lot-to-lot variability that can complicate long-term studies or multi-site collaborations.

Validation data demonstrate reliable performance in immunofluorescence and flow cytometry applications. Immunofluorescence staining of A549 cells at 1:60 dilution reveals clear nuclear localization consistent with NEUROD1's function as a transcription factor. Flow cytometry analysis using SH-SY5Y neuroblastoma cells at 1:50 dilution shows a distinct positive population shift compared to isotype control, confirming specific detection in this neuronal cell model. These validated cell lines provide relevant starting points for researchers working in cancer biology and neuroscience contexts.

The unconjugated format allows flexibility in secondary antibody selection, enabling researchers to optimize detection for their specific imaging or cytometry platforms. Recommended working dilutions of 1:50–1:200 for both immunofluorescence and flow cytometry provide a practical range for protocol optimization.

This antibody supports investigations spanning epigenetics, nuclear signaling, cardiovascular research, and metabolic studies where NEUROD1's transcriptional activity influences cellular differentiation and function.

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Target Background

Function(From Uniprot)

Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis.

Gene References into Functions

Data suggest that the first cases of MODY6 identified in Japan are result of missense (p.L157R, c.470T>G) or frameshift mutations (p.H206PfsTer38, c.616_617insC; p.P245RfsTer17, c.734delC; p.H206TfsTer56, c.616delC) in NEUROD1. The probands and affected family members exhibit intrinsically lower capacity of insulin secretion and neurological disorders. [CASE REPORT] PMID:28664602
Data suggest the EGR1-miR-30a-5p-NEUROD1 axis might serve as a promising biomarker for diagnosis and treatment monitoring for schizophrenic patients in acute psychotic state. EGR1 and miR-30a-5p were remarkably downregulated, whereas NEUROD1 was significantly upregulated in PBMNCs from patients in acute psychotic state. PMID:28072411
Mutation in NEUROD1 gene is associated with Maturity Onset Diabetes of the Young. PMID:28095440
the importance of the oligomeric state of CtBP for coactivation of NeuroD1-dependent transcription, was investigated. PMID:27880001
NeuroD1 seemed not sufficient to induce and maintain neuronal differentiation. Induction of neuronal differentiation by overexpression of Neurog1 initiated important steps for the development of glutamatergic neurons such as the spiral ganglion neurons PMID:27423984
detected c.133A > G (p. Ala45Thr) in children with sensorineural hearing loss PMID:26634621
Study reports a family with autosomal dominant diabetes related to a new NEUROD1 mutation, one of very few meeting Maturity Onset Diabetes of the Young criteria. PMID:26773576
RNAi of lentiviral vector target NeuroD can reduce the migration and invasion abilities of PANC-1 cells PMID:24464628
this study concludes that the novel mechanism would regulate the expression of ALK in neuroblastoma and that NeuroD1 should be significantly involved in neuroblastoma tumorigenesis. PMID:25652313
NEUROD1 is important for maintenance of the retina function and partial loss-of-function mutation in NEUROD1 is likely a rare cause of nonsyndromic ARRP. PMID:25477324
Increased expression of NeuroD1 subsequently leads to regulation of expression and function of the nicotinic acetylcholine receptor subunit cluster of alpha3, alpha5, and beta4. PMID:24719457
Transactivation of Ctbp was dependent on the histone H3 lysine 9 (H3K9) demethylase activity of LSD1 facilitates subsequent H3K9 acetylation by the NeuroD1-associated histone acetyltransferase, P300/CBP-associated factor. PMID:24732800
The variant A45T does not play a major role in the development of T2 Diabetes mellitus in East Asian descent. PMID:23203005
Gene expression profiling revealed that permissive lines are typified by lower expression of the early neurogenic transcription factor ASCL1 and, conversely, by higher expression of the late neurogenic transcription factor NEUROD1. PMID:23739064
NeuroD1 regulates survival and migration of neuroendocrine lung carcinomas via signaling molecules TrkB and NCAM. PMID:23553831
The overexpression of NeuroD may contribute to the tumorogenesis and development of pancreatic carcinoma, and is closely correlated to the cancer cell proliferation, p53 signal pathway and neural invasion. PMID:22455846
Combined transfection of the three transcriptional factors, PDX-1, NeuroD1, and MafA, causes differentiation of bone marrow mesenchymal stem cells into insulin-producing cells PMID:22761608
Photosensitive photoreceptor cells can be generated by combinations of transcription factors. The combination of CRX and RX generate immature photoreceptors: and additional NEUROD promotes maturation. PMID:22558175
Most, if not all, nasal chemosensory neurons derive from NeuroD1-expressing globose basal cells of the immediate neuronal precursor variety. PMID:21800309
Findings establish the critical role of the neuronal differentiation factor NeuroD1 in neuroblastoma as well as its functional relationship with the neuronal repellent factor Slit2. PMID:21349947
ATF2 interacts with beta-cell-enriched transcription factors, MafA, Pdx1, and beta2, and activates insulin gene transcription. PMID:21278380
NeuroD alone may not be sufficient to induce regulated insulin release in insulin-producing liver cells. PMID:21084850
Human NeuroD1 under control of the cytokeratin 19 promoter can induce differerentiation of pancreatic epithelial cells into insulin producing cells. PMID:20692411
syndrome resulting from homozygous loss of function mutations in NEUROD1 which is characterized by permanent neonatal diabetes. PMID:20573748
There was no association between methylation and expression in breast tumour specimens, with only 14% exhibiting NEUROD1 expression PMID:19353266
No significant association of NEUROD1 with retinopathy or nephropathy in Croatian patients with type I diabetes PMID:20120526
regulator of insulin transcription PMID:11755474
expression during trophoblast invasion PMID:11900979
Beta-cell dysfunction in late-onset diabetic subjects carrying homozygous mutation in transcription factor NeuroD1. PMID:12200761
The genetic polymorphism in NeuroD is associated with the development of early-onset type 2 diabetes. The presence of Thr45 allele may represent a risk factor for early-onset type 2 diabetes among Chinese. PMID:12476420
Polymorphism Ala45Thr is associated with Type 1 diabetes mellitus in Czech children PMID:12639765
Ala5Thr polymorphism of NeuroD1 plays a role in the risk of NIDDM in the examined Polish population PMID:12861411
NeuroD1/E47 transcription factors up-regulate IA-1 gene expression through the proximal E-box element of the IA-1 promoter PMID:12890672
Ala45 variant of BETA2/NeuroD1 may be associated with IDDM in Caucasians. PMID:12951629
NeuroD1 is differentially expressed in pituitary adenomas and its possible ontogenetic and/or pathogenetic implications in non-corticotroph tumors are discussed. PMID:14759067
No evidence of Ala(45)Thr polymorphism of the NeueroD gene and type 1 diabetes. PMID:15047635
Focus of this review is on recent progress in understanding the important role of BETA2/NeuroD1 in initiating neuronal differentiation and maintaining the nervous system. PMID:15247487
Polymorphism contributes to glucose intolerance in a South Indian population. PMID:15277395
NeuroD controls both common and distinct sets of molecules involved in cell survival and differentiation in different tissue types [review] PMID:15650322
co-expression and functional synergism of these beta-cell enriched transactivators, MafA, Pdx1, and Beta2, are critical for establishing the beta-cell-specific and efficient expression of the insulin gene. PMID:15993959
the SREBP-1c.BETA2.E47 complex is in a DNA looping structure which is required for efficient recruitment of CREB-binding protein/p300 PMID:16055439
we demonstrated that ISL1 and BETA2 could activate insulin gene transcription synergistically. PMID:16321656
Gender-specific association of the Ala45Thr variant of NEUROD1 with Type 1 diabetes in Brazilian women. PMID:16357810
results presented in this study define INSM1 as a transcriptional repressor of the neuroD/b2 gene. The molecular mechanism of INSM1 transcriptional repression is attributed to the recruitment of cyclin D1 and HDAC-1 and -3 PMID:16569215
The NeuroD1-Ala45Thr variation may itself have an important role in susceptibility to or be in disequilibrium with early-onset T2DM in Chinese. The Ala45Thr may affect the onset pattern of T2DM, i.e., early-onset but not late-onset T2DM in Chinese. PMID:16773428
helix-loop-helix (HLH) domain of basic helix-loop-helix (bHLH) family proteins such as NeuroD facilitate protein transduction into various cell lines. PMID:16870135
Expression of NeuroD1 versus chromogranin-A is more frequent in pCA, and correlates to increased indicators of malignancy in moderately to poorly differentiated pCA. PMID:17126478
These results suggest that NeuroD plays an important role in regulated exocytosis by inducing expressions of various components required in the process. PMID:17217914
A study evaluating the extent to which common variation in the six known maturity-onset diabetes of the young (MODY) genes, which cause a monogenic form of type 2 diabetes, is associated with type 2 diabetes is presented. PMID:17327436
Mutation in the NeuroD1/BETA2 gene contributes to the development of diabetes PMID:17440689