NCK2 Recombinant Monoclonal Antibody

Code: CSB-RA957044A0HU

Rate （N/A）
Review （0）

Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, IHC, FC

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

O43639

Target Names

NCK2

Alternative Names

Cytoplasmic protein NCK2 antibody; GRB4 antibody; Growth factor receptor bound protein 4 antibody; NCK adaptor protein 2 antibody; Nck-2 antibody; NCK2 antibody; NCK2_HUMAN antibody; NCKbeta antibody; Non-catalytic region of tyrosine kinase adaptor protein 2 antibody; Noncatalytic region of tyrosine kinase beta antibody; SH2/SH3 adaptor protein NCK-beta antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human NCK2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1F5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA957044A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA957044A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing A431 cells stained with CSB-RA957044A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Description

NCK2, also known as NCK adaptor protein 2 or GRB4, serves as a critical signaling hub that links receptor tyrosine kinases to downstream effector pathways. This SH2/SH3 domain-containing adaptor protein plays essential roles in cytoskeletal reorganization, cell migration, and growth factor signaling cascades, making it a valuable target for researchers investigating signal transduction mechanisms, cancer biology, and cellular motility.

This recombinant monoclonal antibody, clone 1F5, offers the experimental consistency that demanding research protocols require. Generated through recombinant technology with a defined sequence, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies. Researchers can confidently design longitudinal studies or multi-site collaborations knowing that antibody performance will remain stable across experiments and reorders.

Validation studies demonstrate reliable performance across multiple detection platforms. Immunohistochemistry testing in paraffin-embedded human tissues, including tonsil and cervical cancer specimens, shows specific staining at 1:100 dilution using citrate buffer antigen retrieval. Flow cytometry analysis using A431 cells confirms intracellular detection capability, with clear separation between specific signal and isotype control when cells are fixed and permeabilized. These validated applications provide flexibility for researchers working with both archival tissue samples and cultured cell populations.

The antibody is supplied in a stabilized liquid format containing glycerol for convenient long-term storage at -20°C or -80°C, ready for immediate use without reconstitution. Affinity purification ensures high purity and minimal background in sensitive applications.

For investigators exploring adaptor protein biology, receptor tyrosine kinase signaling networks, or the molecular mechanisms underlying cell migration and cancer progression, this antibody provides a dependable tool for characterizing NCK2 expression and localization in human samples.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.

Gene References into Functions

This study provides evidence of a possible role of NCK2 as biomarker of ovarian cancer progression. PMID:29218693
There was no difference in the Nck2 mRNA expression between the SLE patients and the healthy subjects. PMID:29911835
Nck1 and Nck2 Interact with WTIP. Nck1/2 integrates nephrin with the Hippo kinase cascade through association with the adaptor protein WTIP. PMID:27033705
Data suggest that PINCH1 and Nck2 critically participate in the regulation of cellular radiosensitivity and EGFR function and downstream signaling in a cellular model of human squamous cell carcinoma. PMID:26004008
Tir-Intimin interaction recruits the Nck adaptor to a Tir tyrosine phosphorylated residue where it activates neural Wiskott-Aldrich syndrome protein (N-WASP). PMID:25482634
Proteasomal degradation of Nck1 but not Nck2 regulates RhoA activation and actin dynamics. PMID:24287595
NCK2 is involved in the susceptibility to opiates addiction. PMID:23533358
The hNck2 SH3 domain also exhibited pH dependent monomer-dimer transition. PMID:23524290
Data show that both HK2 and NCK2 are expressed in the retinal ganglion cell layer. PMID:23349798
Nck2 effectively influences human melanoma phenotype progression. PMID:21992144
p21-Activated kinase 3 (PAK3) protein regulates synaptic transmission through its interaction with the Nck2/Grb4 protein adaptor. PMID:21949127
Nck2 is an adaptor protein composed of 3N-SH3 domains followed by a unique Cterminal SH2 domain. It interacts with PINCH in integrin signal transduction, cell migratio and survival. Review. PMID:21880263
biophysical analysis of Nck2 SH3 domain PMID:19956763
Nck-2 interacts with focal adhesion kinase and modulates cell motility. Using a mutational strategy, the formation of the Nck-2-FAK complex is mediated by interactions involving multiple SH2 and SH3 domains of Nck-2. PMID:11950595
Recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. PMID:12110186
analysis of binding between the Tyr-phosphorylated human ephrinB2 and Nck2 SH2 domain PMID:15764601
Molecular model of the first SRC homology 3 domain of Nck2. PMID:16604428
Nck1 (Nckalpha) and Nck2 (Nckbeta and Grb4): binding specificities of both SH2 domains are essentially indistinguishable PMID:16636066
determined the NMR structures and dynamic properties of the hNck2 SH3 domains and to define their ligand binding preferences with nine proline-rich peptides derived from Wire, CAP-1, CAP-2, Prk, Wrch1, Wrch2, and Nogo PMID:16752908
Data show that Nck (isoforms 1 and 2) as a component of the CReP/PP1c holophosphatase complex contributes to maintain eIF2alpha in a hypophosphorylated state, and modulates translation and eIF2alpha signaling in response to ER stress. PMID:16835242
Demonstrate connection between septins/SOCS7/NCK signaling and the DNA damage response. PMID:17803907
the adaptors Nck and ShcA influenced adherence of S. Typhimurium to non-phagocytic cells. PMID:17906149
Data show that Nck forms a complex with an atypical PxxDY motif of the CD3epsilon tail, which encompasses Tyr166 within the activation motif and a T-cell receptor endocytosis signal. PMID:18555270
Results point to NCK2 as a disease candidate gene and further supports the GLC1B locus as an important genomic region that is associated with the genetic predisposition to glaucoma. PMID:18723748
independent roles and mechanisms of action of Nckalpha and Nckbeta in dermal fibroblast migration, which is critical for wound healing. PMID:19242519