MUC5AC Recombinant Monoclonal Antibody

Code: CSB-RA152151A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, IHC

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Target Background

Product Details

Uniprot NO.

P98088

Target Names

MUC5AC

Alternative Names

MUC5AC antibody; MUC5 antibody; Mucin-5AC antibody; MUC-5AC antibody; Gastric mucin antibody; Major airway glycoprotein antibody; Mucin-5 subtype AC antibody; tracheobronchial antibody; Tracheobronchial mucin antibody; TBM antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human MUC5AC protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

18H7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA152151A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA152151A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Description

MUC5AC is a gel-forming mucin that plays a central role in maintaining mucosal barrier integrity throughout the respiratory and gastrointestinal tracts. As a major secretory component of airway and gastric epithelia, this glycoprotein is essential for protecting underlying tissues from pathogens, particulates, and chemical insults. Aberrant MUC5AC expression has been implicated in numerous pathological conditions, including chronic obstructive pulmonary disease, asthma, and various gastrointestinal malignancies, making it a valuable marker for researchers investigating mucosal biology, inflammatory responses, and cancer progression.

This recombinant monoclonal antibody, clone 18H7, offers the consistency and reliability that demanding experimental workflows require. Generated against a synthetic peptide derived from human MUC5AC, the antibody is produced using recombinant technology, ensuring sequence-defined specificity and eliminating the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG format provides excellent signal amplification when paired with standard secondary detection systems.

Validation studies demonstrate robust performance in immunohistochemistry applications, with successful staining confirmed in paraffin-embedded human stomach tissue and human pancreatic cancer specimens. Using a Leica Bond automated staining platform with citrate buffer antigen retrieval, clear and specific signal was achieved at 1:100 dilution, though working concentrations between 1:50 and 1:200 are recommended to accommodate tissue-specific optimization. The antibody is also validated for ELISA applications, providing flexibility across different experimental approaches.

For researchers studying mucin biology in signal transduction pathways, epithelial barrier function, or tumor microenvironment characterization, this affinity-purified antibody delivers the reproducible performance needed to generate publication-quality data.

Customer Reviews and Q&A

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Target Background

Function(From Uniprot)

Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system. Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD).

Gene References into Functions

S100A12 activates NLPR3 inflammasomes to induce MUC5AC production in airway epithelial cells. ATP induces MUC5AC production in a mechanistically similar mode to S100A12. PMID:29906464
The results of the present study indicate that Munc132 may be an essential regulator of basal MUC5AC exocytosis, while Munc134 appears to be a Munc13 protein subtype that may to be sensitive to hNE stimulation during airway MUC5AC hypersecretion. PMID:29767240
resistin induced MUC5AC and MUC5B expression via activation of different signaling pathways in human airway epithelial cells. PMID:29604272
the splice switch to DeltaC-ZIP2 as well as decreased expression of other ZIPs caused zinc deficiency, which is sufficient for induction of MUC5AC. PMID:29289532
this paper shows that IL-1beta upregulates Muc5ac expression via NF-kappaB-induced HIF-1alpha in asthma PMID:29031476
Data suggest that secreted IL-6 regulates CLB2.0-induced MUC5AC and MUC1 expression via ROS-mediated downregulation of claudin-1 expression to maintain mucus homeostasis in the airway. PMID:28946937
Helicobacter pylori (H. pylori)-CagA significantly upregulated MUC5AC, MUC2, and MUC5B expression in AGS cells, but did not affect E-cadherin and MUC6 expression. PMID:29869461
Oct-4 expression and the reduction of MUC5AC expression may be involved in the progression and an unfavorable prognosis of GC PMID:28762513
smoking might decrease tear secretion, goblet cell density and tear MUC5AC concentration. PMID:27297822
High-Mobility Group Box 1 Upregulates MUC5AC and MUC5B Expression in Primary Airway Epithelial Cells PMID:29286856
Cyclic pressure did not induce MUC5AC secretion in the airway mucus epithelium via Ca(2+)-dependent ATP release, and nearly all Ca(2+) was provided by stored intracellular Ca(2). PMID:27919041
IL-33 induced MUC5AC mRNA and MUC5AC protein, and also goblet cell hyperplasia at air liquid interface culture in human nasal epithelial cells. In addition to that, IL-33 induced MUC5B and FOXA3, and reduces FOXJmRNA. PMID:27776277
MUC5AC has pathologic roles in mechanisms of allergen-induced airway hyperresponsiveness, mucous metaplasia, and airway mucus plugging. PMID:27845589
Leptin knockdown suppressed MUC5AC production and secretion induced by IL-13 in human bronchial epithelial cells. PMID:28942146
The results suggest that the analysis of expression of MUC5AC and TFF1 may be useful for differentiating sessile serrated adenomas/polyps (SSA/Ps) from hyperplastic polyps (HPs). PMID:28430953
The Egr-1 is essential for CSE-induced MUC5AC production in HBE cells likely through interaction with and modulation of AP-1, and re-emphasize targeting Egr-1 as a novel therapeutic strategy for COPD. PMID:28602698
Mucus plugs from individuals with fatal asthma are heterogeneous gels with distinct MUC5AC- and MUC5B-containing domains. Tethering of MUC5AC-containing domains to the epithelium causes mucostasis and likely represents a major cause of mucus plugging in asthma. PMID:27183390
The only individual marker that was found to have direct and strong correlation with the clinical outcome of ampullary carcinoma was MUC5AC (not used in the Ang or Chang panels), with statistically significant survival differences found with various cutoffs tested (for 20% cutoff, 5-y survival, 68% vs. 31%; P=0.0002). In addition, MUC5AC significantly stratified the histologically pancreatobiliary and intestinal cases. PMID:28505002
MUC5AC interacts with integrin beta4 that mediates phosphorylation of FAK at Y397 leading to lung cancer cell migration. PMID:26751774
Results show that the glycoforms of MUC5AC in mucinous cysts of the pancreas display two unusual motifs, terminal alphaGlcNAc and terminal betaGlcNAc. PMID:27992432
MUC5AC expression was significantly higher in pancreatic adenocarcinoma than in healthy controls and chronic pancreatitis patients. PMID:27845339
Cigarette smoke exposure initially triggers the production of mitoROS and subsequently elicits autophagy in airway epithelial cells, which then regulate MUC5AC expression partially through modulation of the JNK signaling and the transcription factor AP-1. PMID:27084849
this study shows that S-allylmercapto-l-cysteine can suppress MUC5AC secretion using the cell model of chronic obstructive pulmonary disease PMID:27517516
This paper aims at developing a quantitative computer-aided methodology for automated identification of mucin areas and its percentage using tissue histological images PMID:26971129
MUC5B was significantly more often detected in middle ear effusion fluid relative to MUC5AC. MUC5AC presence was not significantly different in mucoid and serous fluid. PMID:27729120
outcome, while the gain of aberrant expression of MUC5AC and particularly of MUC6 was associated with favorable outcome in colorectal cancer, notably in intermediate stages II and III PMID:27298226
Low mucin1 expression is associated with peritoneal dissemination of gastrointestinal cancer. PMID:27193208
The HNE-TACE signalling pathway has an important role in the process of MUC5AC overexpression in chronic rhinosinusitis. PMID:26881964
Knockdown of MUC5AC in SW620 cells remarkably suppressed cell vitality and promoted apoptosis and G1 cell cycle arrest, resulting in the impaired ability of colony formation. The inhibition of MUC5AC in SW620 cells dramatically repressed the cell migration and invasion. PMID:27610469
These results suggest that IFN-gamma represses MUC5AC expression, disturbing binding of Sp1 to its target sequences. PMID:27324793
this study shows that SYK increased MUC5AC expression via ERK2 and p38 MAPK signaling pathways in airway epithelial cells PMID:26980390
The interference of p38 gene expression inhibited the release of IL-1beta and TNF-alpha in cultured cells. It also depressed both mRNA and protein levels of MUC5A. PMID:26722604
The expression of messenger RNAs is inhibited by EGFR tyrosine kinase inhibitor PMID:26867523
is a major component of the mucus produced by airway epithelial cells. PMID:26100173
Review-Meta-analysis: decreased Muc5AC expression might be a poor prognostic predictor for gastric cancer. PMID:26420972
Data show that verproside inhibits tumor necrosis factor alpha-induced mucin 5AC (MUC5AC) expression through NF-kappa B pathway. PMID:26318254
Overexpression of MUC5AC has been described in idiopathic pulmonary fibrosis lungs. Read More: http://www.atsjournals.org/doi/full/10.1164/rccm.201507-1322LE#.V2WAGNLrtNs PMID:26871672
The expression of ALPi and MUC5AC in cocultures of Caco-2 and HT29 cells developed for permeability studies is reported. PMID:26299896
MUC5AC hypomethylation is predictive of serrated lesions with malignant potential PMID:26476272
EGF removal or tyrphostin AG1478 treatment of differentiating air-liquid interface cultures from asthmatic children would result in a reduction of epithelial goblet cells and mucus secretion. PMID:26057128
These data lend support to the notion that beta2AR-agonists may contribute to asthma exacerbations by increasing mucin production via activation of beta2ARs on epithelial cells. PMID:26161982
The measurement of the CA 19-9 antigen on individual proteins could improve performance over the standard CA 19-9 assay. PMID:22220206
Studied the effects of 15-HETE on MUC5AC expression and related pathways. PMID:25649981
Increased expression of MUC5AC was associated with bacterial biofilm formation in chronic rhinosinusitis patients. PMID:25638393
Leukotriene E4 induces MUC5AC release from human airway epithelial NCI-H292 cells PMID:25838093
Results showed that MUC5AC expression is correlated to the tumor stage where advanced gastric cancers present reduced levels of MUC5AC and that Gli regulates its expression via interaction with a highly conserved sequence in its promoter region. PMID:25166306
MUC5AC expression in ovarian mucinous tumors did not change from benign to malignant tumors. PMID:25298197
Common genetic variations in the MUC5AC gene are not related to helicobacter pylori serologic status. PMID:25605164
The genetic length variants in the central repetitive region of MUC5AC were not associated with meconium ileus in cystic fibrosis. PMID:24920497
Data suggest that determination of mucin 5AC (MUC5AC) methylation status may be useful for understanding and predicting the natural history of colorectal cancers (CRC). PMID:25403854

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Subcellular Location

Secreted.

Tissue Specificity

Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum.

Database Links

HGNC: 7515

UNIGENE: Hs.534332

KEGG: hsa:4586

STRING: 9606.ENSP00000435591

OMIM: 158373

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