MFN1 Recombinant Monoclonal Antibody

Code: CSB-RA574476A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

Q8IWA4

Target Names

MFN1

Alternative Names

Mitofusin-1 (EC 3.6.5.-) (Fzo homolog) (Transmembrane GTPase MFN1), MFN1

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human MFN1

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

11G8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Overlay Peak curve showing HepG2 cells stained with CSB-RA574476A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

Mitofusin-1 (MFN1) is a transmembrane GTPase essential for mitochondrial outer membrane fusion, a process critical for maintaining mitochondrial network integrity, cellular metabolism, and energy homeostasis. Dysfunction in MFN1-mediated fusion has been implicated in neurodegenerative conditions, metabolic disorders, and cellular stress responses, making it a compelling target for researchers investigating mitochondrial dynamics and their downstream consequences in disease pathology.

This recombinant monoclonal antibody, clone 11G8, offers the reproducibility and consistency that demanding experimental workflows require. Because it is derived from a defined sequence and produced recombinantly, researchers can expect uniform performance across lots, eliminating the variability that often complicates long-term studies or multi-site collaborations. The rabbit IgG format, raised against a synthetic peptide from human MFN1, provides reliable specificity for human samples.

Validation by flow cytometry demonstrates clear detection of MFN1 in HepG2 cells, a human hepatocellular carcinoma line frequently used in metabolic and mitochondrial research. The flow cytometry protocol, performed at a 1:50 dilution with fixed and permeabilized cells, showed distinct positive signal separation from isotype control, confirming specific intracellular detection. This makes the antibody well-suited for quantitative single-cell analysis of MFN1 expression levels across experimental conditions.

The antibody is supplied in a glycerol-containing buffer optimized for long-term stability at freezer temperatures, supporting extended project timelines without compromising reagent integrity. For researchers exploring mitochondrial biology, neurodegeneration, metabolic regulation, or signal transduction pathways involving organelle dynamics, this antibody provides a dependable tool for interrogating MFN1 expression and function in human cellular systems.

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Target Background

Function(From Uniprot)

Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Membrane clustering requires GTPase activity. It may involve a major rearrangement of the coiled coil domains. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks (in vitro). Has low GTPase activity.

Gene References into Functions

The results lead to a revised understanding of Mfn 1 as single-spanning outer membrane proteins with an Nout-Cin orientation, providing functional insight into the IMS contribution to redox-regulated fusion events. PMID:29212658
The results may suggest that TP53BP1 and MFN1 frameshift mutations and their intratumoral heterogeneity (ITH) could contribute to cancer development by inhibiting the TSG activities. PMID:30082159
The results show that a metabolic shift from glycolysis in young to mitochondrial respiration in old normal human fibroblasts occurs during chronological lifespan, and MFN1 and OPA1 regulate this process. PMID:28758339
mitochondria elongation under hypoxic condition is regulated through SIRT1-mediated MFN1 deacetylation and accumulation. PMID:28669827
Regulation of Mfn1 by MGRN1 and the proteasome modulates mitochondrial fusion. PMID:27713096
SLC25A46 is a new component in mitochondrial dynamics that serves as a regulator for MFN1/2 oligomerization. PMID:28057766
MFN1-positive expression could be seen mainly in ganglion cells after 1 week of minus lens intervention, and with time extension, more and more positive cells appeared in the rod-cone cell and bipolar cell layer, and this phenomenon could not be found in the normal control eyes. PMID:27609161
crystal structures of engineered human MFN1 containing the GTPase domain and a helical domain during different stages of GTP hydrolysis; mechanistic model for MFN1-mediated mitochondrial tethering is proposed; results shed light on the molecular basis of mitochondrial fusion and mitofusin-related human neuromuscular disorders PMID:28114303
These results suggest that MFN tethers apposing membranes, likely through nucleotide-dependent dimerization. PMID:27920125
Improper transcriptional (in)activation of mitofusin-1 and dynamin-related protein 1 during early in vitro embryo development is associated with a decrease in mitochondrial membrane potential and with embryo fragmentation. PMID:25033890
miR-19b targets 3'UTR sequences of Mfn1 genes inhibit the expression of Mfn1 PMID:24824927
In a amyotrophic lateral sclerosis transgenic mouse model, Mfn1 is significantly increased in spinal cord. PMID:23713734
A novel role for the endoplasmic reticulum-associated Gp78 ubiquitin ligase and the Mfn1 mitochondrial fusion factor in mitophagy. PMID:23427266
Knock-out of mitofusin protein Mfn1 increased the frequency of mitochondrial fission with increased lifetime of unpaired events whereas deletion of both Mfn1 and Mfn2 resulted in an instable dynamics. PMID:22649485
These results collectively suggest a role for Mfn1 in regulating the activation of Bax on the outer mitochondrial membrane in a GTPase-dependent manner. PMID:22484496
mitochondrial dynamics, particularly those mediated by the mitofusins, play a role in endothelial cell function and viability. PMID:21839087
Patterned Purkinje cell degeneration is dependent on caspase activation, leading to the marked decrease of mitofusion 1 in the transgenic Harlequin cerebellum. PMID:20974255
Our data supports a model whereby the translocation of parkin to damaged mitochondria induces the degradation of mitofusin 1 leading to impaired mitochondrial fusion PMID:21615408
The impact of mutations in endogenous PINK1 and Parkin on the ubiquitination of mitochondrial fusion and fission factors and the mitochondrial network structure, was investigated. PMID:21408142
Gbeta2 also regulated the mobility of Mfn1 on the surface of the mitochondrial membrane and affected the mitochondrial fusion. PMID:20981029
Ubiquitination of several mitochondrial proteins, including mitofusin 1 and mitofusin 2 were reduced following the silencing of parkin or PINK1. PMID:20871098
Mitofusin degradation by mitochondria-associated Parkin inhibits the fusion of damaged mitochondria with healthy mitochondria to facilitate the selective elimination of the former by autophagy. PMID:21173115
MFN1 is required for both the virus-induced redistribution of IPS-1 and IFN production. PMID:20661427
the NIC-Akt-Mfn signaling cascade identifies a pathway regulating cell-survival, independent of canonical functions associated with NIC activity PMID:20339081
Results show that Fzo homologs mitofusin 1 and 2 are ubiquitous mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. PMID:11950885
Mfn1 mediates mitochondrial fusion in human cells PMID:12475957
OPA1 functionally requires mitofusin 1 to regulate mitochondrial fusion PMID:15509649
Mfn1, Mfn2, NRF-2 and COX IV mRNA were increased 24 h post-exercise in skeletal muscle PMID:15961417
Precise interactions between a few proteins are required for mitochondrial fusion and division. Among them Drp1, Mfn1, Mfn2 and Opal are considered the most important. PMID:17718388
Fis1 and Mfn1 activities influence mitochondrial signal generation thereby insulin exocytosis. PMID:18832378
IL-6 induces Bcl-2 expression to perform cytoprotective functions in response to oxygen toxicity, and that this effect is mediated by alterations in the interactions between Bak and Mfn1/Mfn2. Bcl-2 inhibited the interaction between Bak and Mfn1. PMID:19168699

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Subcellular Location

Mitochondrion outer membrane; Multi-pass membrane protein.; [Isoform 2]: Cytoplasm.

Protein Families

TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family, Mitofusin subfamily

Tissue Specificity

Detected in kidney and heart (at protein level). Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.

Database Links

HGNC: 18262

UNIGENE: Hs.478383

KEGG: hsa:55669

STRING: 9606.ENSP00000263969

OMIM: 608506

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