MBD3 Recombinant Monoclonal Antibody

Code: CSB-RA072670A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human, Mouse, Rat

Application: ELISA, WB, IHC, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

O95983

Target Names

MBD3

Alternative Names

AI181826 antibody; AU019209 antibody; MBD 3 antibody; Mbd3 antibody; MBD3: methyl CpG binding domain protein 3 antibody; MBD3_HUMAN antibody; Methyl CpG binding domain protein 3 antibody; Methyl CpG binding protein MBD3 antibody; Methyl-CpG-binding domain protein 3 antibody; Methyl-CpG-binding protein MBD3 antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human MBD3 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

11A5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
, Positive WB detected in:COLO205 whole cell lysate(30µg)Mouse brainr tissue lysate(30µg), Rat brain lysate(30µg)
All lanes: MBD3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 33 kDa
Observed band size: 33, 35 kDa
Exposure time：60s
IHC image of CSB-RA072670A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing Hela cells stained with CSB-RA072670A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Description

MBD3 (Methyl-CpG-binding domain protein 3) serves as a critical component of the NuRD (nucleosome remodeling and deacetylase) complex, playing essential roles in transcriptional regulation and chromatin remodeling. As a methyl-CpG-binding protein, MBD3 participates in epigenetic mechanisms that control gene expression patterns, making it a valuable target for researchers investigating chromatin dynamics, developmental biology, and disease-associated epigenetic alterations.

This recombinant rabbit monoclonal antibody (clone 11A5) offers the reproducibility and consistency that demanding epigenetics research requires. Generated against a synthetic peptide derived from human MBD3, the recombinant format ensures sequence-defined specificity and eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations.

Validation across multiple platforms demonstrates this antibody's versatility in your experimental workflows. Western blot analysis confirms reliable detection in COLO205 human colorectal carcinoma cells as well as mouse and rat brain tissue lysates, with observed bands at 33 and 35 kDa. The appearance of a secondary band slightly above the predicted 33 kDa molecular weight likely reflects post-translational modifications such as glycosylation or phosphorylation, which are common for chromatin-associated proteins. Immunohistochemistry validation in human placenta tissue sections shows clear nuclear staining patterns consistent with MBD3's chromatin-associated function, while flow cytometry analysis in HeLa cells demonstrates suitability for single-cell quantification approaches.

With confirmed cross-species reactivity spanning human, mouse, and rat samples, this antibody supports comparative studies across model systems commonly used in epigenetic research. Whether investigating NuRD complex function, chromatin remodeling mechanisms, or epigenetic contributions to disease states, this MBD3 antibody provides a dependable tool for your nuclear signaling investigations.

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Target Background

Function(From Uniprot)

Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself. Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides. Recruits histone deacetylases and DNA methyltransferases.

Gene References into Functions

Study have demonstrated that MBD3/ NuRD inhibits formation of liver induced cancer stem cells (iCSCs). In addition, MBD3 suppression induces c-JUN, resulting in the induction of pluripotent genes in iCSCs. PMID:27894081
Suggest that MBD3 exerts influences on both active and silenced genes in glioma cells. PMID:27835581
MBD3 inhibits epithelial-mesenchymal transition in pancreatic cancer cells probably via TGF-beta/Smad signaling and may be a candidate for diagnostics and prognosis of pancreatic cancer. PMID:27898661
Overexpression of MBD3 is associated with neoplasms. PMID:27465550
Insufficient MBD3 induced by small interfering RNA (siRNA) was found to result in a global DNA hypermethylation as well as increased methylation in the promoter CpG islands (CGIs) of a number of cell cycle related genes. PMID:25753672
This study investigates the genetic association between methyl-CpG-binding domain (MBD) gene polymorphisms and schizophrenia. PMID:24849540
These data suggest that MBD3, and by extension the NuRD complex, may have multiple roles in fine tuning expression for both active and silent genes, representing an important step in defining regulatory mechanisms PMID:24385926
reduced mRNA expression of MBD2 and MBD3 is implicated in gastric carcinogenesis. PMID:24338710
MBD3 is enriched at active promoters, whereas MBD2 is bound at methylated promoters and enriched at exon sequences of active genes. PMID:23361464
These factors lead to a binding affinity hierarchy of p66alpha for the different MBD2 homologues (MBD2 approximately MBD3 > MBD3L1 approximately MBD3L2). PMID:23239876
MBD3 mutations are not responsible for ICR1 hypomethylation in Silver-Russell syndrome. PMID:20004753
MBD3 is highly expressed in glioblastome multiforme compared to astrocytoma and anaplastic astrocytoma PMID:11836615
interaction with two highly related p66 proteins PMID:12183469
localization in Aurora-A-positive centrosomes in M phase PMID:12354758
MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing PMID:15701600
MBD3 assembles into mutually exclusive distinct Mi-2/NuRD-like complex, called MBD3/NuRD. PMID:16428440
This is the first demonstration that MBD3 is involved in inducing and maintaining the demethylated state of a specific promoter. PMID:17452452
data demonstrate a causal relationship between MBD3 and DNA demethylation of genomic targets in cells. PMID:18602768
Study demonstrates a direct role of the MBD3, a subunit of nucleosome remodeling and deacetylase corepressor complex complex in aberrant gene repression and transmission of epigenetic repressive marks in acute promyelocytic leukemia. PMID:18644863
These findings collectively support a role for MBD3 in cell cycle progression and cell death as a modulator of HDAC-mediated transcription. PMID:19041848