MBD2 Recombinant Monoclonal Antibody

Code: CSB-RA981891A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, WB, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

Q9UBB5

Target Names

MBD2

Alternative Names

Demethylase antibody; DMTase antibody; MBD 2 antibody; Mbd2 antibody; MBD2_HUMAN antibody; MBD2a antibody; Methyl CpG binding domain protein 2 antibody; Methyl CpG binding protein MBD2 antibody; Methyl-CpG-binding domain protein 2 antibody; Methyl-CpG-binding protein MBD2 antibody; NY CO 41 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human MBD2

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3E5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

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Western Blot
Positive WB detected in: HEK293 whole cell lysate(30µg)
All lanes: MBD2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 29 kDa
Exposure time: 3min
Overlay Peak curve showing Raji cells stained with CSB-RA981891A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

MBD2 (Methyl-CpG-binding domain protein 2) plays a central role in epigenetic gene regulation by recognizing and binding methylated CpG dinucleotides in genomic DNA. As a core component of the NuRD chromatin remodeling complex, MBD2 functions as a transcriptional repressor that interprets DNA methylation patterns to silence gene expression. This positions MBD2 at the intersection of epigenetic research, cancer biology, and developmental studies, where aberrant DNA methylation patterns frequently contribute to disease pathogenesis.

This recombinant monoclonal antibody (clone 3E5) offers the reproducibility essential for epigenetic research, where subtle changes in protein expression can carry significant biological meaning. Generated in rabbit against a synthetic peptide derived from human MBD2, the recombinant format ensures sequence-defined consistency across experiments and eliminates the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations.

Validation studies confirm reliable performance across multiple platforms. In western blot applications, the antibody detects MBD2 in HEK293 whole cell lysates at dilutions of 1:500–1:2000, with an observed band at 29 kDa. The 29 kDa band detected by this recombinant anti-MBD2 antibody in HEK293 lysates corresponds to the endogenously processed, mature form of MBD2 resulting from N-terminal cleavage (or alternative splicing), which migrates faster than the predicted 43 kDa full-length precursor against which the immunizing synthetic peptide was designed. Flow cytometry validation in Raji cells demonstrates clear separation from isotype controls at 1:50–1:200 dilutions, enabling intracellular detection of MBD2 in lymphoid cell populations.

This antibody supports investigations into DNA methylation-mediated gene silencing, chromatin remodeling mechanisms, and the epigenetic alterations underlying cancer progression and cellular differentiation.

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Target Background

Function(From Uniprot)

Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.

Gene References into Functions

Suggest that reduced MBD2 expression could contribute to chronic airway inflammation in COPD. PMID:29535511
knockdown of MBD2 by siRNA attenuated vancomycin-induced apoptosis, caspase activity, and the expression of BAX and cleaved caspase 3. PMID:29022913
CpG and methylation-dependent DNA binding and dynamics of the MBD2 protein at the single-molecule level have been reported. PMID:28637186
Results show that MBD2 mediates epigenetic silencing of Htra1. PMID:27388476
MBD2 has a critical role in 'rewriting' the cancer methylome at specific regulatory regions. PMID:27593931
MBD2 expression was elevated in HCC tissue, which suggesting MBD2 as a candidate prognostic marker of HCC. PMID:27315121
downregulation of miR-221 inhibits cell migration and invasion at least partially through targeting MBD2 in the human OSCC cell line UM1. PMID:26788506
Specifically, methyl-CpG-binding domain protein 2 (MBD2) is revealed to be recruited to DNA damage sites after laser microirradiation, which was mediated through MBD domain and MBD2 C-terminus. PMID:26827827
MBD2 targets short interspersed nuclear elements, but does not exclude RNA Polymerase III. PMID:25798578
The dynamics of MBD2 deposition across methylated DNA regions was associated with the oncogenic transformation of human mammary cells. PMID:26007656
Data suggest that MBD2 binds primarily at highly methylated regions, with a strong preference for CpG islands and highlights that MBD2 binding sites display increased methylation in primary breast cancer tissues as compared to normal mammary cells. PMID:24927503
Biophysical analyses show that the MBD2IDR is an intrinsically disordered region (IDR). However, despite this inherent disorder, MBD2IDR increases the overall binding affinity of MBD2 for methylated DNA. PMID:25753662
This study investigates the genetic association between methyl-CpG-binding domain (MBD) gene polymorphisms and schizophrenia. PMID:24849540
alternatively spliced isoforms support pluripotency of stem cells PMID:24813856
The methylated-DNA binding protein MBD2 enhances NGFI-A (egr-1)-mediated transcriptional activation of the glucocorticoid receptor. PMID:25135974
These data point to a potential new approach in targeting the DNA methylation machinery by combination of MBD2 and DNMT inhibitors. PMID:25178277
MBD2 seems to play a selective role in gene repression depending on the CpG content of the promoter region PMID:23888954
Methylated DNA binding domain protein 2 (MBD2) coordinately silences gene expression through activation of the microRNA hsa-mir-496 promoter in breast cancer cell line. PMID:24204564
reduced mRNA expression of MBD2 and MBD3 is implicated in gastric carcinogenesis. PMID:24338710
global DNA hypomethylation is one of the possible epigenetic variations associated with the complex etiology of TOF and significantly correlates with the aberrant expression of MBD2 mRNA in patients with TOF. PMID:23820632
MBD2 binds a significant fraction of the hypomethylated genes. PMID:23955541
Abnormal expression levels of DNA (cytosine-5-)-methyltransferase 1 and MBD2 mRNA may be important causes of the global hypomethylation observed in CD4+T cells in systemic lupus erythematosus. PMID:23127209
In metastatic colorectal cancer cells, reduced levels of miR-221* and miR-224 increase levels of MBD2, thereby decreasing expression of the metastasis suppressor maspin. PMID:23770133
MBD3 is enriched at active promoters, whereas MBD2 is bound at methylated promoters and enriched at exon sequences of active genes. PMID:23361464
These factors lead to a binding affinity hierarchy of p66alpha for the different MBD2 homologues (MBD2 approximately MBD3 > MBD3L1 approximately MBD3L2). PMID:23239876
repressive functions of MBD2-containing NuRD complexes are dependent on cooperative interactions between the major domains of CHD4 with histones and DNA and on binding of methylated DNA by MBD2 PMID:23071088
MiR-373 behaves as a direct transcriptional target and negative regulator of MBD2 activity through a feedback loop of CpG island methylation in hilar cholangiocarcinoma PMID:22876037
The association between MBD2 binding and transcriptional repression weakened as the distance between binding site and TSS increased, suggesting that MBD2 represses transcriptional initiation PMID:22048253
A two-stage association study identifies methyl-CpG-binding domain protein 2 gene polymorphisms as candidates for breast cancer susceptibility. PMID:22258532
These results show a role for MBD2 in cancer progression and provide support for the prospect of targeting MBD2 therapeutically in aggressive breast cancers. PMID:21693597
MBD2 overexpression during gliomagenesis may drive tumor growth by suppressing the antiangiogenic activity of a key tumor BAI1. PMID:21724586
human epsilon-globin gene is subject to multilayered silencing mediated in part by MBD2 PMID:21296012
In hilar cholangiocarcinoma, down-expression of miR-373 leads to increase of MBD2, which in turn suppresses the methylation-mediated gene such as RASSF1A. PMID:21086164
This study demonstrated that patients with SLE had a significantly lower level of DNA methylation than the controls, and that expression of both DNMT1 and MBD2 mRNA was significantly increased in the SLE patients compared with controls. PMID:21078759
Expression level of MBD2 is significantly lower in endometriotic lesions compared with disease-free controls. PMID:21316665
miR-373 is a methylation-mediated gene and the implication of MBD2 in methylation-mediated suppression of miR-373 plays an important role in tumourigenesis and development in hilar cholangiocarcinoma. PMID:21165562
The anti-TNFalpha biological agents do not seem to affect DNA methylation and mRNA expressions of DNMT1 and MBD2 in RA PMID:20937307
MBD2 and ERalpha drive opposite effects on pS2 expression, which are associated with specific steady state levels of histone H3 acetylation and methylation marks PMID:20300195
Methyl-CpG binding domain protein 2 represses transcription from hypermethylated pi-class glutathione S-transferase gene promoters in hepatocellular carcinoma cells PMID:11960994
MBD2 protein activates CpG sites within the promoter region of reporter genes PMID:12177048
interaction with two highly related p66 proteins PMID:12183469
interacts with latency-associated nuclear antigen of Kaposi's Sarcoma-associated herpesvirus (KSHV)to tether KSHV to cell chromosomes PMID:12388720
MBDin relieves MBD2 repression potential and reactivates transcription from methylated promoters PMID:12588985
Results show that methyl-CpG binding domain protein 1 (MBD1) is expressed in tumor cells, but methyl-CpG binding domain protein 2 (MBD2) and methyl CpG binding protein 2 (MeCP2) are not. PMID:12646234
MBD2a and RNA helicase A cooperatively enhanced CREB-dependent gene expression. PMID:12665568
MBD2 has a role in the methylation-mediated inhibition of ribosomal RNA gene expression PMID:14610093
Antisenses oligoDNA suppresses tumor growth in nude mice, a potential anticaner therapy approach. PMID:14688029
MBD2 gene expression may be significant factor in tumorigenesis. PMID:15112265
role of MBD3L1 as a methylation-dependent transcriptional repressor that may interchange with MBD3 as an MBD2-interacting component of the NuRD complex PMID:15456747
MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing PMID:15701600