MAPK1/MAPK3 Recombinant Monoclonal Antibody

Code: CSB-RA984568A0HU

Rate （N/A）
Review （0）

Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human, Mouse

Application: ELISA, WB, IHC, FC

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

P27361 P28482

Target Names

MAPK1/MAPK3

Alternative Names

Mitogen-activated protein kinase 3 (MAP kinase 3) (MAPK 3) (EC 2.7.11.24) (ERT2) (Extracellular signal-regulated kinase 1) (ERK-1) (Insulin-stimulated MAP2 kinase) (MAP kinase isoform p44) (p44-MAPK) (Microtubule-associated protein 2 kinase) (p44-ERK1), MAPK3, ERK1 PRKM3; Mitogen-activated protein kinase 1 (MAP kinase 1) (MAPK 1) (EC 2.7.11.24) (ERT1) (Extracellular signal-regulated kinase 2) (ERK-2) (MAP kinase isoform p42) (p42-MAPK) (Mitogen-activated protein kinase 2) (MAP kinase 2) (MAPK 2), MAPK1, ERK2 PRKM1 PRKM2

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from Human MAPK1/MAPK3

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

29B10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: THP-1 whole cell lysate, HEPG2 whole cell lysate,MCF7 whole cell lysate,293 whole cell lysate,K562 whole cell lysate,PC-3 whole cell lysate, Mouse brain tissue lysate
All lanes: ERK1/2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42,44 kDa
Observed band size: 42,44 kDa
IHC image of CSB-RA984568A0HU diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.64% DAB.
Overlay Peak curve showing Hela cells surface stained with CSB-RA984568A0HU (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

ERK1 and ERK2, encoded by MAPK3 and MAPK1 respectively, represent the terminal kinases of the classical MAP kinase cascade, serving as critical integration points for mitogenic signals, stress responses, and differentiation cues. These serine/threonine kinases phosphorylate hundreds of cytoplasmic and nuclear substrates, making them central to understanding cell proliferation, survival, and oncogenic transformation. Given their near-ubiquitous involvement in signal transduction research, reliable detection of both isoforms is essential across diverse experimental contexts.

This recombinant rabbit monoclonal antibody, clone 29B10, offers the reproducibility that ERK1/2 studies demand. Developed against a synthetic peptide derived from human MAPK1/MAPK3, the antibody is produced through recombinant technology, ensuring sequence-defined consistency between lots. This eliminates the variability that can confound longitudinal studies or multi-site collaborations where identical reagent performance is critical.

Validation across multiple platforms demonstrates broad experimental utility. Western blot analysis at 1:1000 dilution reveals clean detection of both the 42 kDa ERK2 and 44 kDa ERK1 bands, matching predicted molecular weights, across an extensive panel including THP-1, HepG2, MCF7, HEK293, K562, and PC-3 cell lysates, as well as mouse brain tissue. This cross-species reactivity between human and mouse samples supports translational research workflows. Immunohistochemistry validation in paraffin-embedded human stomach tissue confirms suitability for tissue-based studies, while flow cytometry analysis of HeLa cells demonstrates clear signal separation from isotype controls.

Whether investigating MAPK pathway dysregulation in cancer models, characterizing upstream signaling events, or validating pathway inhibitor efficacy, this antibody provides the technical foundation for confident ERK1/2 detection across western blotting, immunohistochemistry, and flow cytometry applications.