MAP2K6/MAP2K3 Recombinant Monoclonal Antibody

Code: CSB-RA590869A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, WB, IHC, FC

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Target Background

Product Details

Uniprot NO.

P46734 P52564

Target Names

MAP2K6/MAP2K3

Alternative Names

AW212142 antibody; dual specificity mitogen activated protein kinase kinase 3 antibody; Dual specificity mitogen-activated protein kinase kinase 3 antibody; MAP kinase kinase 3 antibody; map2k3 antibody; MAPK ERK kinase 3 antibody; MAPK/ERK kinase 3 antibody; MAPKK 3 antibody; MAPKK3 antibody; MEK 3 antibody; MEK3 antibody; Mitogen activated protein kinase kinase 3 antibody; MKK 3 antibody; MKK3 antibody; mMKK3b antibody; MP2K3_HUMAN antibody; PRKMK 3 antibody; PRKMK3 antibody; protein kinase, mitogen-activated, kinase 3 antibody; SAPK kinase 2 antibody; SAPKK 2 antibody; SAPKK2 antibody; Stress activated protein kinase kinase 2 antibodyDual specificity mitogen activated protein kinase kinase 6 antibody; Dual specificity mitogen-activated protein kinase kinase 6 antibody; MAP kinase kinase 6 antibody; MAP2K6 antibody; MAPK/ERK kinase 6 antibody; MAPKK 6 antibody; MAPKK6 antibody; MEK 6 antibody; MEK6 antibody; Mitogen Activated Protein Kinase Kinase 6 antibody; MKK 6 antibody; MKK6 antibody; MP2K6_HUMAN antibody; PRKMK6 antibody; protein kinase antibody; Protein kinase mitogen activated kinase 6 antibody; protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6) antibody; SAPK kinase 3 antibody; SAPKK-3 antibody; SAPKK3 antibody; SKK3 antibody; Stress-activated protein kinase kinase 3 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human MAP2K6/MAP2K3

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2B4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: COLO-205 whole cell lysate(30µg), Hela whole cell lysate(30µg),HEK293 whole cell lysate(30µg),HT-29 whole cell lysate(30µg),Raji whole cell lysate(30µg)
All lanes: MEK3/MEK6 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 37，39 kDa
Observed band size: 37，39 kDa
Exposure time：2min
IHC image of CSB-RA590869A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA590869A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing PC-3 cells stained with CSB-RA590869A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

MAP2K6 and MAP2K3, also known as MEK6 and MEK3 respectively, are dual-specificity kinases that serve as critical upstream activators of the p38 MAPK signaling pathway. These kinases play essential roles in cellular stress responses, inflammatory signaling, and apoptotic regulation, making them important targets for researchers investigating signal transduction mechanisms in cancer biology, immunology, and stress-related cellular processes.

This recombinant monoclonal antibody, developed using rabbit host immunization and clone 2B4, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined genetic sequences, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures comparable results across experiments. The antibody has been affinity-purified to maximize specificity while minimizing background interference.

Validation studies demonstrate robust performance across multiple applications in human samples. Western blot analysis confirms detection of both MEK3 and MEK6 at their predicted molecular weights of 37 and 39 kDa across diverse cell lines including COLO-205, HeLa, HEK293, HT-29, and Raji, indicating reliable performance in colorectal cancer, cervical carcinoma, embryonic kidney, and B-cell lymphoma research models. Immunohistochemistry validation in paraffin-embedded human glioma cancer and small intestine tissue sections demonstrates suitability for tissue-based studies examining MEK3/MEK6 expression patterns. Flow cytometry analysis using PC-3 prostate cancer cells further extends the antibody's utility for single-cell quantification of intracellular kinase levels.

This versatile reagent supports investigations into p38 MAPK pathway dysregulation, stress-activated signaling cascades, and therapeutic target validation across oncology and inflammatory disease research programs.