LRP2 Recombinant Monoclonal Antibody

Code: CSB-RA288700A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

P98164

Target Names

LRP2

Alternative Names

Low-density lipoprotein receptor-related protein 2 (LRP-2) (Glycoprotein 330) (gp330) (Megalin), LRP2

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human LRP2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

10B5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol Flow Cytometry (FC) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Overlay Peak curve showing MCF7 cells stained with CSB-RA288700A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

The production of the LRP2 recombinant monoclonal antibody involves a well-defined and rigorous process to ensure its quality and specificity. Initially, B cells are isolated from an immunized animal, with a synthesized peptide derived from human LRP2 used as the immunogen. Total RNA is extracted from the B cells, and cDNA is synthesized through reverse transcription. The LRP2 antibody genes are amplified using PCR with primers specific to the antibody constant regions and inserted into an expression vector. This vector is then introduced into host cells, enabling the production of the LRP2 recombinant monoclonal antibody. The antibody is harvested from the cell culture supernatant and subjected to affinity chromatography for purification, resulting in a highly purified preparation. Extensive characterization, including ELISA and FC analysis, is performed to validate the antibody's specificity and functionality, ensuring its ability to specifically recognize human LRP2 protein.

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Target Background

Function(From Uniprot)

Multiligand endocytic receptor. Acts together with CUBN to mediate endocytosis of high-density lipoproteins. Mediates receptor-mediated uptake of polybasic drugs such as aprotinin, aminoglycosides and polymyxin B. In the kidney, mediates the tubular uptake and clearance of leptin. Also mediates transport of leptin across the blood-brain barrier through endocytosis at the choroid plexus epithelium. Endocytosis of leptin in neuronal cells is required for hypothalamic leptin signaling and leptin-mediated regulation of feeding and body weight. Mediates endocytosis and subsequent lysosomal degradation of CST3 in kidney proximal tubule cells. Mediates renal uptake of 25-hydroxyvitamin D3 in complex with the vitamin D3 transporter GC/DBP. Mediates renal uptake of metallothionein-bound heavy metals. Together with CUBN, mediates renal reabsorption of myoglobin. Mediates renal uptake and subsequent lysosomal degradation of APOM. Plays a role in kidney selenium homeostasis by mediating renal endocytosis of selenoprotein SEPP1. Mediates renal uptake of the antiapoptotic protein BIRC5/survivin which may be important for functional integrity of the kidney. Mediates renal uptake of matrix metalloproteinase MMP2 in complex with metalloproteinase inhibitor TIMP1. Mediates endocytosis of Sonic hedgehog protein N-product (ShhN), the active product of SHH. Also mediates ShhN transcytosis. In the embryonic neuroepithelium, mediates endocytic uptake and degradation of BMP4, is required for correct SHH localization in the ventral neural tube and plays a role in patterning of the ventral telencephalon. Required at the onset of neurulation to sequester SHH on the apical surface of neuroepithelial cells of the rostral diencephalon ventral midline and to control PTCH1-dependent uptake and intracellular trafficking of SHH. During neurulation, required in neuroepithelial cells for uptake of folate bound to the folate receptor FOLR1 which is necessary for neural tube closure. In the adult brain, negatively regulates BMP signaling in the subependymal zone which enables neurogenesis to proceed. In astrocytes, mediates endocytosis of ALB which is required for the synthesis of the neurotrophic factor oleic acid. Involved in neurite branching. During optic nerve development, required for SHH-mediated migration and proliferation of oligodendrocyte precursor cells. Mediates endocytic uptake and clearance of SHH in the retinal margin which protects retinal progenitor cells from mitogenic stimuli and keeps them quiescent. Plays a role in reproductive organ development by mediating uptake in reproductive tissues of androgen and estrogen bound to the sex hormone binding protein SHBG. Mediates endocytosis of angiotensin-2. Also mediates endocytosis of angiotensis 1-7. Binds to the complex composed of beta-amyloid protein 40 and CLU/APOJ and mediates its endocytosis and lysosomal degradation. Required for embryonic heart development. Required for normal hearing, possibly through interaction with estrogen in the inner ear.

Gene References into Functions

megalin is critical for mitochondrial biology; mitochondrial intracrine signaling is a continuum of the retrograde early endosome-to-Golgi-Rab32 pathway and defects in this pathway may underlie disease processes in many systems. PMID:29916093
patients with OCRL-1 mutations or type 1 Dent disease showed abnormally low levels of urinary A-megalin PMID:27766457
An Emerging Role for Megalin as a Regulator of Protein Leak in Acute Lung Injury. PMID:29090957
Review of LRP2 function. LRP2 functions are crucial for developmental processes in a number of tissues, including the brain, the eye, and the heart, and defects in this receptor pathway are the cause of devastating congenital diseases in humans. PMID:26872844
A novel LRP2 missense variant rs17848169 (N2632D) was found to be associated with lower risk for T2D-ESRD in this population. PMID:27197912
we discovered one novel locus (LRP2; most significant single nucleotide polymorphism rs12988804) that reached genome-wide significance in predicting relapse risk (HR=2.18, p=3.30x10(-8)). PMID:28739605
miR-148b directly down-regulates renal megalin expression. PMID:28331063
Exocytosis-mediated urinary C-megalin excretion is associated with the development and progression of diabetic nephropathy in T2DM, particularly due to megalin-mediated lysosomal dysfunction in proximal tubules. PMID:28289043
The studies suggest that impaired endocytosis of megalin/cubilin ligands, hemoglobin and albumin, rather than heme toxicity, may be the cause of tubular proteinuria in sickle cell disease patients. PMID:28356267
VDR and MEGALIN gene variations can alter age-related cognitive trajectories differentially between men and women among African American urban adults, specifically in global mental status and domains of verbal fluency, visual/working memory, and executive function. PMID:28446629
the main role for placental megalin is not to mediate uptake of nutrients from the maternal bloodstream; results point toward novel and complex functions for megalin in the cytotrophoblasts. PMID:27798286
specific miRNA-146a regulation may contribute to Alzheimer's disease by downregulating the Lrp2/Akt pathway PMID:27241555
abundances of megalin and Dab2 (p = 0.046) were reduced in infected placentas from women with LBW deliveries PMID:27072056
homozygous Asp3779Asn and a hemizygous Ile262Met mutations in the LRP2 and TSPYL2 genes, respectively, in a Pakistani family with two boys affected with mild nonsyndromic intellectual disability PMID:26529358
The megalin expression appears to vary inversely with gestational age with the greatest expression noted in the most premature samples. Age-dependent differences in placental megalin may therefore influence fetal exposure. PMID:25304941
we are the first to identify the association between LRP2 and gout in a Chinese population and to confirm this association in Asians. PMID:26147675
Two novel LRP2 mutations, a homozygous nonsense mutation and a missense mutation in two unrealted families with Donnai-Barrow syndrome. PMID:25682901
Loss of LRP2 is associated with buphthalmos PMID:26439398
melanoma cell expression of LRP2/megalin significantly decreases melanoma cell proliferation and survival rates. PMID:25585665
levels of urinary C-megalin are associated with histological abnormalities in adult IgAN patients PMID:25502002
A new mutation in LRP2 causes a predominantly ocular phenotype suggestive of Stickler syndrome. PMID:23992033
LRP2 sequencing reveals multiple rare variants associated with urinary trefoil factor-3 PMID:24876117
Serum uric acid-related gene LRP2 is not involved in gout susceptibility. PMID:24366390
Transgenic/knock-out megalin-deficient mice develop anxiety behavior and impaired learning, as described in Alzheimer's disease. PMID:24254699
Association of the T-allele of a single nucleotide polymorphism in LRP2 with gout risk in the Maori and Pacific subjects was consistent with this allele increasing serum urate in Japanese individuals PMID:24286387
LOS treatment decreased microalbuminuria induced by Cd apparently through a cubilin receptor-dependent mechanism but independent of megalin. PMID:24093454
Data suggest that endodermal layer of yolk sac and syncytiotrophoblast/cytotrophoblast cells of placental villi express megalin mRNA/protein; expression of megalin protein (but not mRNA) is up-regulated as gestation/placentation progresses. PMID:23978537
This review explores current evidence linking megalin expression and function to the development, diagnosis, and progression of acute kidney injury --{REVIEW} PMID:24197071
Megalin and Dab2 were expressed in prostate and colon epithelial cells, which was markedly enhanced following treatment with retinoic acid PMID:23909735
The hypothalamic clusterin-low-density lipoprotein receptor-related protein-2 axis is a novel anorexigenic signalling pathway. PMID:23673647
results suggested that GSTT1 wild genotype and C-allele of megalin gene rs2228171 SNPs might be risk factors for cisplatin-induced ototoxicity PMID:23274376
Using NMR titration data in HADDOCK, we have generated a three-dimensional model describing the complex between megalin and gentamicin. PMID:23275343
Expression of megalin and cubilin is decreased during experimental endotoxemia, which may contribute to an increase in urine levels of albumin during acute renal failure. PMID:22437417
analysis of rare disease variants in LRP2, a gene linked and associated with autism spectrum disorders PMID:22578327
This study confirmed that LRP2 rs2544390 C/T at intron 1 was associated with serum uric acid levels among Japanese males with SLC22A12 258WW, SLC2A9 rs11722228C allele, ABCG2 126QQ and 141Q allele. PMID:22565184
A total of 330 Chinese female-offspring nuclear families with 1088 individuals and 400 Chinese male-offspring nuclear families with 1215 individuals were genotyped at six tag single nucleotide polymorphisms of the LRP2 gene. PMID:22174918
Sex-specific VDR and Megalin gene variations can modify age-related cognitive decline among US adults. PMID:22170372
megalin and cubilin are involved in the metabolism of vitamin D by reabsorbing vitamin D binding protein; dysfunction of these receptors is likely to be associated with the development of vitamin D deficiency in patients with chronic kidney disease PMID:21595846
allele (A) of the rs3755166 polymorphism within LRP2 gene may contribute to Alzheimer's disease risk in the Chinese Han Population PMID:20971101
in anagen VI hair follicles megalin was found in all keratinocytes of the distal region PMID:21104416
Data show that MT-I + II and megalin are significantly altered in CNS lymphoma relative to controls. PMID:20038220
No defect in the trafficking or function of megalin upon OCRL1 knockdown. PMID:19940034
Megalin and cubilin: multifunctional endocytic receptors. A review. PMID:11994745
This study reveals that LRP2 is a major autoantigen in rheumatoid arthritis and probably drives the production of anti-LRP2 autoantibodies, which may play pathological roles by inhibiting the reabsorbing function of LRP2. PMID:12723989
megalin has a role in thyroid homeostasis with possible implications in thyroid diseases PMID:14657389
a binding affinity of disabled homolog 2 mitogen-responsive phosphoprotein interaction domain for megalin CT of K(D) = 2.6 x 10(-7) +/- 5.3 x 10(-8) PMID:15134832
megalin endocytosed NGAL by a mechanism completely blocked by an antibody against megalin PMID:15670845
Further studies on the intracellular molecular signalling associated with megalin-mediated metabolic pathways may lead to the development of novel strategies for the treatment of nephropathies related to diabetes and metabolic syndrome. (Review) PMID:16174284
This review focuses on the involvement of megalin during embryonic development and its interactions with the developmental morphogen sonic hedgehog. PMID:16828734
Results show that the PPPSP motif and GSK3 activity are critical to allow megalin phosphorylation and also negatively regulate the receptor's recycling. PMID:17555532