LGR4 Recombinant Monoclonal Antibody

Code: CSB-RA883618MA1HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, IF

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Target Background

Product Details

Uniprot NO.

Q9BXB1

Target Names

LGR4

Alternative Names

BNMD17 antibody; G protein coupled receptor 48 antibody; G-protein coupled receptor 48 antibody; GPR48 antibody; Leucine-rich repeat-containing G protein-coupled receptor 4 antibody; Leucine-rich repeat-containing G-protein coupled receptor 4 antibody; LGR4 antibody; LGR4_HUMAN antibody

Species Reactivity

Human

Immunogen

Recombinant Human LGR4 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

17E4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunofluorescence (IF) Protocol

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Immunofluorescence staining of Hela cell with CSB-RA883618MA1HU at 1:30 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific.

Description

LGR4, also known as GPR48, is a leucine-rich repeat-containing G protein-coupled receptor that plays a pivotal role in Wnt/β-catenin signaling modulation. This receptor has emerged as a significant research target due to its involvement in diverse biological processes including embryonic development, tissue homeostasis, and stem cell maintenance. Understanding LGR4 function provides valuable insights into developmental biology and has implications for research into tissue regeneration and related pathological conditions.

This recombinant monoclonal antibody, clone 17E4, offers the reproducibility and consistency that demanding signal transduction research requires. Because the antibody sequence is defined and produced through recombinant technology, you can expect uniform performance across experiments and over time, eliminating the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations. The human IgG1 isotype format and affinity chromatography purification ensure high specificity for your human LGR4 detection needs.

Validation studies demonstrate effective performance in immunofluorescence applications, with testing in HeLa cells showing clear cellular localization when used at dilutions between 1:50 and 1:200. The immunofluorescence protocol employed standard fixation with 4% formaldehyde and 0.2% Triton X-100 permeabilization, making it straightforward to integrate into established laboratory workflows. The antibody is also validated for ELISA, providing flexibility for researchers who need both quantitative and imaging-based detection methods.

Supplied in a glycerol-based PBS buffer with Proclin 300 preservative, this antibody is ready for immediate use in your signal transduction studies. Whether investigating Wnt pathway dynamics, receptor trafficking, or LGR4's role in cellular signaling networks, this recombinant antibody delivers the reliability your research demands.

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Target Background

Function(From Uniprot)

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Its function as activator of the Wnt signaling pathway is required for the development of various organs, including liver, kidney, intestine, bone, reproductive tract and eye. May also act as a receptor for norrin (NDP), such results however require additional confirmation in vivo. Required during spermatogenesis to activate the Wnt signaling pathway in peritubular myoid cells. Required for the maintenance of intestinal stem cells and Paneth cell differentiation in postnatal intestinal crypts. Acts as a regulator of bone formation and remodeling. Involved in kidney development; required for maintaining the ureteric bud in an undifferentiated state. Involved in the development of the anterior segment of the eye. Required during erythropoiesis. Also acts as a negative regulator of innate immunity by inhibiting TLR2/TLR4 associated pattern-recognition and proinflammatory cytokine production. Plays an important role in regulating the circadian rhythms of plasma lipids, partially through regulating the rhythmic expression of MTTP.

Gene References into Functions

Loss of LGR4 abrogated RSPO3-regulated osteogenesis and RSPO3-induced ERK1/2 signalling inhibition. PMID:28220828
Luciferase reporter assay was performed to confirm that LGR4 is a direct target gene of miR-137 with a potential binding site in the 3'UTR of LGR4. PMID:29786747
the host protein leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4) is essential for VSV and VSV-G pseudotyped lentivirus (VSVG-LV) to infect susceptible cells. PMID:28842478
High LGR4 expression is associated with osteoarthritis. PMID:28777797
Data (including data from studies using transgenic/knockout mice) suggest that LGR4 is key protein necessary for prostate cancer epithelial-mesenchymal transition and metastasis; LGR4 expression is elevated in human prostate cancer cell lines with metastatic potential; LGR4 silencing in prostate cancer cell line impairs cell migration and invasion without affecting cell proliferation. PMID:28768769
LGR4 is another receptor for RANKL. LGR4 competes with RANK to bind RANKL and suppresses canonical RANK signaling during osteoclast differentiation. PMID:27064449
The LGR4 A750T variant is correlated with central obesity-related characteristics in young subjects. PMID:27925416
Lgr4 is a critical positive factor for skin tumorigenesis by mediating the activation of MEK1/ERK1/2 and Wnt/beta-catenin pathways. PMID:27693558
miR-34a gene expression upregulation inhibits ARPE-19 cell proliferation, migration and adhesion partly by suppressing LGR4 expression. PMID:27977785
Lgr4 activates Jmjd2a/AR signaling pathway to promote interaction AR with PSA promoter, causing reduction of prostate cancer apoptosis and cell cycle arrest. PMID:27743893
A mechanistic understanding of RANKL-LGR4 interaction has provided new insight into LGR4 mediated RANKL signaling in osteoporosis and other diseases PMID:27178473
miR-218 directly targets LGR4 and modulated the PI3K/Akt and Wnt/beta-catenin pathways in the LNCaP-IL-6+ cells. PMID:26986507
LGR4 promotes tumorigenesis of prostate cancer via PI3K/Akt signaling pathway. PMID:25636507
These findings suggest that aberrant RSPO3-LGR4 signaling potentially acts as a driving mechanism in the aggressiveness of Keap1-deficient lung ADs. PMID:25531322
the LGR4-Rspo1 complex crystal structure shows divergent mechanisms of ligand recognition by leucine-rich repeat G-protein-coupled receptors PMID:25480784
our results suggest a previously unknown Stat3-LGR4 molecular network, which may control osteosarcoma development and progression PMID:24455684
RSPO-LGR4 not only induces the clearance of RNF43/ZNRF3 to increase Wnt receptor levels but also recruits IQGAP1 into the Wnt signaling complex. PMID:24639526
Lgr4, which regulates eye, kidney, testis, ovary, and uterine organ development as well as mental development through genetic and epigenetic surveillance, is a novel candidate gene for the pathogenesis of AGR syndrome PMID:24519938
GPR48 overexpression promotes cancer cell proliferation via activation of Wnt signaling. PMID:24083742
Lgr4 overexpression promoted glioma cell proliferation through activation of Wnt signaling. PMID:24083766
Upregulation of GPR48 resulted in increased phosphorylation of glycogen synthase kinase 3beta. PMID:23803691
A functional low-frequency human LGR4 variant (A750T) has been associated with body mass index in a Chinese obese-versus-control study. PMID:24212090
Multi-functional norrin is a ligand for the LGR4 receptor. PMID:23444378
the c.376C>T mutation is associated with low BMD and osteoporotic fractures, electrolyte imbalance, late onset of menarche, reduced testosterone levels, and an increased risk of squamous cell carcinoma of the skin and biliary tract cancer PMID:23644456
Lgr4/Gpr48 plays a critical role in modulating the TLR2/4 signaling pathway and represents a useful therapeutic approach of targeting Lgr4/Gpr48 in TLR2/4-associated septic shock and autoimmune diseases. PMID:23589304
R-Spondin potentiates Wnt/beta-catenin signaling through orphan receptors LGR4 and LGR5 PMID:22815884
LGR4 and LGR5 bind the R-spondins with high affinity and mediate the potentiation of Wnt/beta-catenin signaling by enhancing Wnt-induced LRP6 phosphorylation. PMID:21693646
GPR48 may play an important role in invasiveness and metastasis of carcinoma and might represent a potential prognostic marker or therapeutic target. PMID:17178856
Testicular efferent ducts in the mutant mouse are dramatically shortened and less convoluted than in the wild-type animal, providing an explanation to the phenotype observed in LGR4KO. PMID:19301269
Data show that GPR48 shRNA can inhibit in vitro invasion and in vivo metastasis of HeLa cells efficiently. PMID:19550120

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 1 family

Tissue Specificity

Expressed in multiple steroidogenic tissues: placenta, ovary, testis and adrenal. Expressed also in spinal cord, thyroid, stomach, trachea, heart, pancreas, kidney, prostate and spleen.

Database Links

HGNC: 13299

UNIGENE: Hs.502176

KEGG: hsa:55366

STRING: 9606.ENSP00000368516

OMIM: 166710

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