KRT6A Recombinant Monoclonal Antibody

Code: CSB-RA788545A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, IF, FC

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Target Background

Product Details

Uniprot NO.

P02538

Target Names

KRT6A

Alternative Names

Keratin, type II cytoskeletal 6A (Cytokeratin-6A) (CK-6A) (Cytokeratin-6D) (CK-6D) (Keratin-6A) (K6A) (Type-II keratin Kb6) (allergen Hom s 5), KRT6A, K6A KRT6D

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human KRT6A

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

21E2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Immunofluorescence staining of A549 with CSB-RA788545A0HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 519-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing Hela cells stained with CSB-RA788545A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

Keratin 6A is a type II intermediate filament protein that plays essential roles in epithelial cell structure and wound healing responses. Expressed prominently in stratified epithelia and rapidly induced during tissue repair, KRT6A serves as a valuable marker for studying epithelial differentiation, barrier function, and cellular responses to injury. Its involvement in cytoskeletal organization makes it particularly relevant for researchers investigating epithelial biology and signal transduction pathways.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human KRT6A, offers the reproducibility and consistency that demanding research applications require. As a sequence-defined recombinant clone, it eliminates the lot-to-lot variability that can complicate longitudinal studies, ensuring your experimental conditions remain stable across projects. The rabbit IgG format provides excellent signal amplification while maintaining specificity for your target.

Validation studies demonstrate reliable performance in both immunofluorescence and flow cytometry applications. Immunofluorescence staining of A549 human lung carcinoma cells reveals clear cytoplasmic localization patterns at dilutions ranging from 1:50 to 1:200, with optimal signal achieved using standard fixation and blocking protocols. Flow cytometric analysis using HeLa cells confirms specific detection with distinct peak separation from isotype controls, supporting quantitative analysis of KRT6A expression at the single-cell level.

The unconjugated format provides flexibility for pairing with your preferred secondary detection systems, while the glycerol-stabilized formulation supports long-term storage stability. Whether you are characterizing epithelial cell populations, investigating wound healing mechanisms, or exploring signal transduction pathways, this antibody delivers the specificity and consistency needed for meaningful experimental outcomes.

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Target Background

Function(From Uniprot)

Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair.

Gene References into Functions

Manipulating K6a phosphorylation or ubiquitin-proteasome system (UPS) activity may provide opportunities to harness the innate immunity of epithelia against infection. PMID:29191848
Several missense polymorphisms in KRT6A, KRT6B and KRT6C that lead to a higher risk for dental caries. PMID:29357356
KRT6A genetic mutation is associate with the development of Pachyonychia Congenita in patients in Australia. PMID:27041546
we here describe a family with pachyonychia congenita K6a, manifesting atypical symptoms of impaired wound healing and cheilitis. PMID:24708461
Keratin-derived antimicrobial peptides (KDAMPs) and their synthetic analogs exhibit antimicrobial activity against bacterial pathogens. PMID:23006328
Genotype-phenotype correlations among PC patients with codon-125 mutation in KRT16 were established, while the phenotypes caused by the IVS8-2A>C mutation in KRT6A need further studies to confirm the rare feature of fissured tongue PMID:22668561
We observed a higher likelihood of oral leukokeratosis in individuals harboring KRT6A mutations. PMID:22264670
Phenotypic differences exist between KRT6A and KRT16 mutations support adoption of a new classification system. PMID:22098151
This report is the first case of pachyonychia congenita with laryngeal obstruction in which the gene mutation has been established (a deletional mutation in keratin 6a). PMID:21554383
Focal palmoplantar keratoderma is associated with mutations in keratin K6c in 3 families. 2 unrelated families have Asn172 del and the other has a deletion of AA 462-470. Review. PMID:20470930
these data highlight the possibility of a physiological role for K6/K16 heterodimers in keratinocyte cell migration, in addition to the heterodimer's known functions in cell differentiation and mechanical resilience. PMID:20403371
The mutation of 521T--> C in the K6A gene is the causing mutation in pachyonychia congenita type I. PMID:20140871
Mutations Y465H and N171D of the KRT16A gene were detected in the sporadic pachyonychia congenita cases. PMID:19806570
Four new missense and five known mutations in K6a, one new deletion and three previously identified missense mutations in K16, plus one known mutation in K17 are reported in pachyonychia congenita. PMID:17719747
PC-1 is due to mutations of the KRT16 gene or its expression partner KRT6A, wheres PC-2 is caused by mutations in the KRT17 or KRT6B genes. PMID:18489596
Three novel and four recurrent keratin 6A (KRT6A) mutations were found in Chinese patients with pachyonychia congenita type 1 PMID:19416275
Rapamycin selectively inhibits expression of an inducible keratin (K6a) in human keratinocytes and improves symptoms in pachyonychia congenita patients. PMID:19699613