KRT16 Recombinant Monoclonal Antibody

Code: CSB-RA250248A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, WB, IF, FC

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Target Background

Product Details

Uniprot NO.

P08779

Target Names

KRT16

Alternative Names

Keratin, type I cytoskeletal 16 (Cytokeratin-16) (CK-16) (Keratin-16) (K16), KRT16, KRT16A

Species Reactivity

Human

Immunogen

A synthesized peptide from human KRT16 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

15D3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: MCF-7 whole cell lysate(30µg)
All lanes:Cytokeratin 16 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time：1min40s
Immunofluorescence staining of HepG2 cell with CSB-RA250248A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing Hela cells stained with CSB-RA250248A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

Keratin 16 is a type I intermediate filament protein that plays a critical role in epithelial cell structure and stress response. Expressed primarily in stratified epithelia, KRT16 serves as a valuable marker for studying keratinocyte differentiation, wound healing, and inflammatory skin conditions. Its upregulation in hyperproliferative states makes it particularly relevant for researchers investigating psoriasis, wound repair mechanisms, and epithelial cancers.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human KRT16, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly, you can expect reliable performance across experiments and over time, eliminating the lot-to-lot variability that can complicate long-term studies or multi-site collaborations.

Validation testing demonstrates robust performance across multiple applications, giving you flexibility in your experimental design. Western blot analysis of MCF-7 whole cell lysate reveals a clean band at the predicted molecular weight of 51 kDa, confirming specific target recognition. For cellular localization studies, immunofluorescence staining has been validated in HepG2 cells, while flow cytometry analysis in HeLa cells shows clear positive signal separation from isotype controls, enabling quantitative assessment of KRT16 expression at the single-cell level.

Whether you are characterizing epithelial differentiation programs, investigating keratin expression patterns in cancer cell lines, or exploring the molecular basis of skin disorders, this antibody provides a dependable tool for your research. The validated performance across Western blot, immunofluorescence, and flow cytometry applications supports diverse experimental approaches within a single, well-characterized reagent.

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Target Background

Function(From Uniprot)

Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.

Gene References into Functions

Study identified iRHOM2 as a novel regulator of K16 in humans and mice, with important implications for palmoplantar keratodermas, wound healing, inflammatory skin disease and cancers. PMID:28128203
results suggest a conditional regulation of KRT16 gene by ATF4 that may be inhibited in normal cells, but engaged during cancer progression. Potential roles of KRT16, FAM129A and HKDC1 genes upregulation in adaptive stress responses and pathologies are discussed PMID:29420561
inflammatory cytokines promoted Nrf2 nuclear translocation in psoriatic epidermis, which led to elevated expression of K6, K16, and K17, thus promoting keratinocyte proliferation and contributing to the pathogenesis of psoriasis. PMID:28576737
The wider spectrum of KRT16 mutations suggests that changes in codons 125, 127, and 132 are most commonly responsible for PC-1 and that proline substitution mutations at codons 127 or 128 may produce more severe disease PMID:24357266
analysis of a new p.Leu421Pro (c.1262T>C) mutation in the highly conserved helix termination motif of K16 in a large Spanish family that may have a role in pachyonychia congenita PMID:24118415
Keratin 16 regulates innate immunity in response to epidermal barrier breach. PMID:24218583
Data established a seven-gene (AR, ESR2, GATA3, GBX2, KRT16, MMP28 and WNT11) prognostic signature to define a subset of triple-negative breast cancer (TNBC). PMID:23549873
Patients with p.Asn125Asp and p.Arg127Pro mutations in KRT16 exhibited more severe disease than patients carrying p.Asn125Ser and p.Arg127Cys mutations in terms of age of onset of symptoms, extent of nail involvement, and impact on daily quality of life. PMID:21160496
Data suggest that an immune response to trichohyalin and K16 may have a role in the pathogenesis of the enigmatic disorder. PMID:20722389
coinheritance of mutations in KRT16 and filaggrin may aggravate the pachyonychia congenita phenotype PMID:19785597
disease-associated keratin 16 expression is induced by epidermal growth factor is regulated through cooperation of transcription factors Sp1 and c-Jun PMID:12954631
K16 expression is observed in non-lesional psoriatic skin and may serve as a marker of preclinical psoriasis. PMID:15239676
ERK2-mediated C-terminal serine phosphorylation of p300 was a key event in the regulation of EGF-induced keratin 16 expression. PMID:17623675
DPPIV-expression and enzyme activity, Ki-67 antigen and K16 are significantly upregulated in the centre and inner margin of the psoriatic lesion compared to clinically uninvolved skin and the healthy volunteers skin. PMID:18496701
Antikeratin 16 autoAbs are involved in psoriasis, by exaggerating the innate immune response of keratinocytes. PMID:18557933
Infection by HPV stimulates the expression of K16 PMID:19515043