KDM1B Recombinant Monoclonal Antibody

Code: CSB-RA958367A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, WB

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Target Background

Product Details

Uniprot NO.

Q8NB78

Target Names

KDM1B

Alternative Names

Lysine-specific histone demethylase 1B (EC 1.-.-.-) (Flavin-containing amine oxidase domain-containing protein 1) (Lysine-specific histone demethylase 2), KDM1B, AOF1 C6orf193 LSD2

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human KDM1B

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

15F3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate
All lanes: KDM1B antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 93, 66, 20 kDa
Observed band size: 90-95, 17-28 kDa

Description

The creation of the KDM1B recombinant monoclonal antibody involves a complex process with several steps. To begin with, the KDM1B monoclonal antibody is harvested, and its gene sequence is determined. Following that, a vector containing the KDM1B monoclonal antibody gene is constructed and transfected into a host cell line for culture. A synthesized peptide, which is derived from human KDM1B, is utilized as an immunogen during KDM1B monoclonal antibody synthesis. The KDM1B recombinant monoclonal antibody is purified by affinity chromatography and subjected to analysis for specificity using ELISA and WB applications. It only detects human KDM1B protein.

KDM1B, also known as LSD1, is a lysine-specific demethylase that catalyzes the removal of methyl groups from histone H3 lysine 4 (H3K4) and lysine 9 (H3K9), leading to transcriptional repression. KDM1B plays a role in the regulation of gene expression, embryonic development, and differentiation. It has been implicated in the progression of various diseases, including cancer and neurodegenerative disorders.

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Target Background

Function(From Uniprot)

Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4.

Gene References into Functions

These results suggest that LSD2 achieves a promoting effect on small cell lung cancer by indirectly regulating TFPI2 expression through the mediation of DNMT3B expression or through the regulation of the demethylation of H3K4me1 in the promoter region of the TFPI2 gene. PMID:29845195
This review focuses on published small-molecule inhibitors targeted at the two flavin adenine dinucleotide-dependent lysine demethylases, lysine-specific demethylases 1 and 2, and how the inhibitors interact with the tertiary structures of the enzymes. PMID:28277979
These results demonstrate an important role for LSD2 in regulation of DNA methylation and gene silencing in breast cancer. PMID:24924415
Histone demethylase LSD2 acts as an E3 ubiquitin ligase and inhibits cancer cell growth through promoting proteasomal degradation of OGT. PMID:25773598
Using transcriptome and chromatin immunoprecipitation-sequencing analyses, the study revealed that LSD2 represses the genes involved in lipid influx and metabolism through demethylation of histone H3K4. PMID:25624347
regulation of tissue factor pathway inhibitor-2 (TFPI-2) expression by lysine-specific demethylase 1 and 2 PMID:25036127
The zinc finger-SWIRM-oxidase domains is required for KDM1B demethylase activity and the binding of FAD. PMID:23266887
Human LSD2/KDM1b/AOF1 regulates gene transcription by modulating intragenic H3K4me2 methylation. PMID:20670891