KAT7 Recombinant Monoclonal Antibody

Code: CSB-RA179517A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human, Mouse

Application: ELISA, WB, IHC

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Target Background

Product Details

Uniprot NO.

O95251

Target Names

KAT7

Alternative Names

Histone acetyltransferase KAT7 (EC 2.3.1.48) (Histone acetyltransferase binding to ORC1) (Lysine acetyltransferase 7) (MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2) (MYST-2), KAT7, HBO1 HBOa MYST2

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human KAT7 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8C6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: K562 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg)
All lanes: KAT7 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 71 kDa
Observed band size: 75 kDa
Exposure time：2min
IHC image of CSB-RA179517A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Description

KAT7, also known as HBO1 or MYST2, is a histone acetyltransferase that plays a fundamental role in chromatin remodeling and transcriptional regulation. As a member of the MYST family of acetyltransferases, KAT7 is essential for DNA replication licensing, cell proliferation, and embryonic development. Its involvement in acetylating histones H3 and H4 makes it a compelling target for researchers investigating epigenetic mechanisms, cell cycle control, and developmental biology.

This recombinant monoclonal antibody, generated in rabbit against a synthetic peptide derived from human KAT7, offers the consistency and reproducibility that demanding epigenetic research requires. Because the antibody sequence is defined and production occurs through recombinant technology, researchers can expect reliable lot-to-lot performance across extended studies and collaborative projects. The monoclonal nature ensures recognition of a single epitope, providing specificity that supports confident data interpretation.

Validation studies demonstrate robust performance across multiple applications. In western blot analysis, the antibody detects KAT7 in both human K562 and mouse NIH/3T3 whole cell lysates at dilutions between 1:500 and 1:2000, confirming cross-species reactivity. The observed band at approximately 75 kDa, slightly higher than the predicted 71 kDa molecular weight, likely reflects post-translational modifications such as glycosylation or phosphorylation commonly associated with this protein. Immunohistochemistry validation in paraffin-embedded human testis tissue shows clear staining at 1:100 dilution, demonstrating suitability for tissue-based studies examining KAT7 expression patterns.

This antibody serves researchers exploring histone modification pathways, cancer biology where KAT7 dysregulation has been implicated, and stem cell differentiation studies where chromatin remodeling is central to cellular fate decisions.

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Target Background

Function(From Uniprot)

Catalytic subunit of histone acetyltransferase HBO1 complexes, which specifically mediate acetylation of histone H3 at 'Lys-14' (H3K14ac), thereby regulating various processes, such as gene transcription, protein ubiquitination, immune regulation, stem cell pluripotent and self-renewal maintenance and embryonic development. Some complexes also catalyze acetylation of histone H4 at 'Lys-5', 'Lys-8' and 'Lys-12' (H4K5ac, H4K8ac and H4K12ac, respectively), regulating DNA replication initiation, regulating DNA replication initiation. Specificity of the HBO1 complexes is determined by the scaffold subunit: complexes containing BRPF scaffold (BRPF1, BRD1/BRPF2 or BRPF3) direct KAT7/HBO1 specificity towards H3K14ac, while complexes containing JADE (JADE1, JADE2 and JADE3) scaffold direct KAT7/HBO1 specificity towards histone H4. H3K14ac promotes transcriptional elongation by facilitating the processivity of RNA polymerase II. Acts as a key regulator of hematopoiesis by forming a complex with BRD1/BRPF2, directing KAT7/HBO1 specificity towards H3K14ac and promoting erythroid differentiation. H3K14ac is also required for T-cell development. KAT7/HBO1-mediated acetylation facilitates two consecutive steps, licensing and activation, in DNA replication initiation: H3K14ac facilitates the activation of replication origins, and histone H4 acetylation (H4K5ac, H4K8ac and H4K12ac) facilitates chromatin loading of MCM complexes, promoting DNA replication licensing. Acts as a positive regulator of centromeric CENPA assembly: recruited to centromeres and mediates histone acetylation, thereby preventing centromere inactivation mediated by SUV39H1, possibly by increasing histone turnover/exchange. Involved in nucleotide excision repair: phosphorylation by ATR in response to ultraviolet irradiation promotes its localization to DNA damage sites, where it mediates histone acetylation to facilitate recruitment of XPC at the damaged DNA sites. Acts as an inhibitor of NF-kappa-B independently of its histone acetyltransferase activity.; Plays a central role in the maintenance of leukemia stem cells in acute myeloid leukemia (AML). Acts by mediating acetylation of histone H3 at 'Lys-14' (H3K14ac), thereby facilitating the processivity of RNA polymerase II to maintain the high expression of key genes, such as HOXA9 and HOXA10 that help to sustain the functional properties of leukemia stem cells.

Gene References into Functions

acts in concert with SNF2H-ACF1 to make the chromosome structure more accessible to canonical nucleotide excision repair factors PMID:28719581
Hbo1 is another ERalpha coactivator that ubiquitinates ERalpha PMID:28769019
Histone acetyltransferase binding to ORC1 (HBO1) is upregulated in bladder cancer. PMID:28796367
The results demonstrate some connections between KAT7 upregulated in RA SFs and RA progression and present the inhibition of KAT7 activity as a novel therapeutic target for interfering RA disease. PMID:28552525
Structural and mechanistic insights into regulation of HBO1 histone acetyltransferase activity by BRPF2 have been presented. PMID:28334966
KAT7-containing acetyltransferases associating with the Mis18 complex provides competence for histone turnover/exchange activity on alphoid DNA and prevents Suv39h1-mediated heterochromatin invasion into centromeres. PMID:27270040
the possible binding sites and biological functions of HBO1, were identified. PMID:27247147
Data show that HTLV-1 basic leucine zipper (bZIP) factor (HBZ) represses p53 activity by direct inhibition of the histone acetyltransferase (HAT) activity of p300/CBP and the HAT activity of HBO1: [HBZ] PMID:26625199
This study demonstrates that CRL4(DDB2) is a novel ubiquitin ligase of HBO1. PMID:26572825
Taken together, MYST2 is a repressed growth suppressor in AML mediating reduced acetylation of histone 4 at residue 5 and is associated with inferior AML patient survival. PMID:26072331
Myst2-mediated histone acetylation may be required for recruitment of Oct4 to the Nanog promoter PMID:25743411
Increase in the number of cells with cytoplasmic JADE1S correlated with activation of tubular cell proliferation and inversely correlated with the number of cells with nuclear JADE1S, supporting role of HBO1-JADE1 shuttling during organ regeneration. PMID:24739512
Hbo1 modulated transcription by estrogen receptor-alpha. Hbo1 contributes to degradation of ER-alpha by ubiquitination through lysine 48. PMID:24125069
Phosphorylated Hbo1 expression is associated with breast cancer. PMID:23955388
alternate choice of subunits associated with HBO1 can switch its specificity between H4 and H3 tails PMID:24065767
Gemcitabine resistance is dependent on Plk1-mediated phosphorylation of Orc2 and Hbo1. PMID:23188630
Modulation of Fbxw15 levels was able to differentially regulate histone H3K14 acetylation and cellular proliferation by altering HBO1 levels. PMID:23319590
The protein domains required for the assembly and function of HBO1/ING complexes were shown. Multiple PHD finger domains in different subunits bound the histone H3 N-terminal tail with a specificity towards lysine 4 methylation. PMID:22144582
JNK1 phosphorylation of Cdt1 inhibits recruitment of HBO1 histone acetylase and blocks replication licensing in response to stress PMID:21856198
HBO1 was an important downstream molecule of ERalpha. PMID:21040551
HBO1 promote MCM loading in vivo involves the stimulation of large-scale chromatin decondensation to allow access to the underlying DNA substrate. PMID:20980834
results show that ING4 is a bivalent reader of the chromatin H3K4me3 modification and suggest a mechanism for enhanced targeting of the HBO1 complex to specific chromatin sites PMID:20053357
H4 acetylation at origins by HBO1 is critical for DNA replication licensing. PMID:20129055
inhibition by Set/TAF-1beta inhibits active demethylation of DNA, integrating DNA methylation and transcriptional silencing PMID:11978794
ordered recruitmenet to thyroid hormone-responsive promoters in vivo PMID:12034878
Hbo1 is a positive regulatory factor for prereplicative complex assembly. PMID:16428461
histone acetyltransferase binding to ORC-1 might play an important physiological role in human progesterone receptor signaling PMID:16645042
reveal a direct regulatory connection between p53-responsive stress signaling and Hbo1-dependent chromatin pathways PMID:17954561
Hbo1 depletion results in decreased DNA replication and a failure of Mcm complex binding to chromatin, both of which can be partially rescued by the ectopic expression of WT Hbo1 but not Hbo1-S57A PMID:18250300
Jade-1/1L are crucial co-factors for HBO1-mediated histone H4 acetylation PMID:18684714
HBO1 associates with replication origins specifically during the G1 phase of the cell cycle in a manner that depends on the replication licensing factor Cdt1, but is independent of the Cdt1 repressor Geminin. PMID:18832067
Overexpression of MYST2 is associated with breast cancer. PMID:19372580
Immunohistochemistry for Hbo1 in 11 primary human tumor types revealed strong Hbo1 protein expression in carcinomas of the testis, ovary, breast, stomach/esophagus, and bladder. PMID:19393168