IKBKG Recombinant Monoclonal Antibody

Code: CSB-RA931025A0HU

Rate （N/A）
Review （0）

Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, IF, FC

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

Q9Y6K9

Target Names

IKBKG

Alternative Names

IkB kinase associated protein 1 antibody; IkB kinase subunit gamma antibody; Inhibitor of nuclear factor kappa B kinase subunit gamma antibody; AMCBX1 antibody; FIP 3 antibody; FIP-3 antibody; FIP3 antibody; Fip3p antibody; I kappa B kinase gamma antibody; I-kappa-B kinase subunit gamma antibody; IkB kinase gamma subunit antibody; IkB kinase subunit gamma antibody; IkB kinase-associated protein 1 antibody; Ikbkg antibody; IKK gamma antibody; IKK-gamma antibody; IKKAP1 antibody; IKKG antibody; IMD33 antibody; Incontinentia pigmenti antibody; Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase gamma antibody; Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, gamma antibody; Inhibitor of nuclear factor kappa-B kinase subunit gamma antibody; IP antibody; IP1 antibody; IP2 antibody; IPD2 antibody; NEMO antibody; NEMO_HUMAN antibody; NF kappa B essential modifier antibody; NF kappa B essential modulator antibody; NF-kappa-B essential modifier antibody; NF-kappa-B essential modulator antibody; ZC2HC9 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human IKBKG

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4D11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Immunofluorescence staining of Hela cell with CSB-RA931025A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing HepG2 cells stained with CSB-RA931025A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

IKBKG, also known as NEMO (NF-kappa-B essential modulator), serves as the regulatory subunit of the IKK complex, playing an indispensable role in NF-κB signaling pathway activation. This scaffolding protein coordinates the assembly and activation of the IKK complex, which phosphorylates IκB proteins to trigger their degradation and subsequent NF-κB nuclear translocation. Mutations in IKBKG are associated with incontinentia pigmenti and various immunodeficiency syndromes, making it a critical target for researchers investigating inflammatory responses, immune regulation, and related genetic disorders.

This recombinant monoclonal antibody, clone 4D11, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human IKBKG and produced using recombinant technology in rabbit host, this antibody provides sequence-defined specificity with minimal lot-to-lot variation. The affinity-chromatography purification ensures high purity, supporting reliable results across your experimental timeline.

Validation studies demonstrate robust performance in immunofluorescence and flow cytometry applications. Immunofluorescence staining of HeLa cells reveals clear cytoplasmic localization patterns at dilutions ranging from 1:50 to 1:200, while flow cytometric analysis of HepG2 cells shows distinct positive population shifts compared to isotype controls, confirming specific detection of endogenous IKBKG. These human cell line validations support the antibody's utility for studying NF-κB pathway dynamics in cellular models.

Whether investigating inflammatory signaling mechanisms, characterizing immune cell activation states, or exploring the molecular basis of IKBKG-associated pathologies, this antibody provides a dependable tool for advancing your research into this essential signaling hub.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin plays a key role in IKK activation by multiple signaling receptor pathways. Can recognize and bind both 'Lys-63'-linked and linear polyubiquitin upon cell stimulation, with a much higher affinity for linear polyubiquitin. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination.; (Microbial infection) Also considered to be a mediator for HTLV-1 Tax oncoprotein activation of NF-kappa-B.

Gene References into Functions

Computational analysis identified two miR-107 binding sites in the 3'UTR of IKBKG suggesting that IKBKG expression is regulated by miR-107. PMID:30396951
Human IKKgamma does not interact with mammalian Atg8-family proteins. PMID:29097655
Data suggest the angiopoietin-like 8 (ANGPTL8)/p62-IKKgamma axis as a negative feedback loop that regulates NF-kappaB activation, and extends the role of selective autophagy in fine-tuned inflammatory responses. PMID:29255244
this study demonstrates immunodeficiency in two female patients with Incontinentia Pigmenti with heterozygous NEMO mutation diagnosed by lipopolysaccharide unresponsiveness PMID:28702714
GSK-3beta is critically important for ordered NF-kappaB signalling through modulation of NEMO phosphorylation. PMID:27929056
HOTAIR operates the action of IKKalpha, IKKbeta, IKKgamma in liver cancer stem cells PMID:27367027
the present study found that loss of the NEMO-SHARPIN interaction impaired recruitment of truncated NEMO forms into punctuate structures that are transiently formed on cell stimulation and thus led to a defect in linear ubiquitination PMID:28249776
NEMO was critically involved in the cGAS-STING pathway. PMID:28939760
Results show that NEMO's expression is regulated by ASAP3 which it interacts directly with it reducing its poly-ubiquitinylation. PMID:28502111
E+P treatment of breast cancer cells increased ER binding to the NEMO promoter, thereby increasing NEMO expression. PMID:28515148
Hematopoietic stem cell transplantation can cure most clinical features of patients with a variety of IKBKG mutations. PMID:28679735
Authors show that NEMO stabilizes HIFalpha via direct interaction and independently of NF-kappaB signaling in vitro. NEMO prolongs tumor cell survival via regulation of apoptosis and activation of epithelial-to-mesenchymal transition, facilitating tumor metastasis. PMID:26500060
The results demonstrate the the first example of father-to-daughter transmission of IP in which a pathogenic mutation in IKBKG has been demonstrated PMID:27037530
Molluscum contagiosum virus MC005 inhibited NF-kappaB proximal to the IkappaB kinase (IKK) complex, and unbiased affinity purification revealed that MC005 interacts with the IKK subunit NEMO (NF-kappaB essential modulator). PMID:28490597
These data suggest that molluscum contagiosum virus MC159 competitively binds to NEMO to prevent cIAP1-induced NEMO polyubiquitination. PMID:28515292
High IKBKG expression is associated with multiple myeloma. PMID:27454822
Our findings shed light on the nature of the interaction between NEMO and poly-ubiquitin, suggesting that NEMO is differentially regulated by poly-ubiquitin chain length and that this regulation occurs via a modulation of the available equilibrium of conformational states, rather than gross structural change PMID:27028374
FADD, as well as NEMO, is a substrate for LUBAC ubiquitin ligase (E3) complex, composed of the HOIP, HOIL-1L, and SHARPIN subunits. PMID:28189684
Consistent with experimental evidence, the zinc ion is essential for mechanical stabilization of the functional, folded conformation of NEMO. PMID:28035815
Herein, our simulations of the zinc finger NEMO (2JVX) using multiple simulations of length 15, 30, 1000, and 3000 ns are analyzed to provide clarity on this point. PMID:25734227
Deletion of exons 4 to 10 (NEMODelta4-10) accounts for about 80% of cases (familial and sporadic) of Incontinentia pigmenti. PMID:26564087
results further reveal that cFLIPLrequires the linear ubiquitin chain assembly complex and the kinase TAK1 for activation of the IKK kinase PMID:26865630
USP18 negatively regulates NF-kappaB signaling by targeting TAK1 and NEMO for deubiquitination through distinct mechanisms. PMID:26240016
A missense mutation in IKBKG causes a Nager syndrome or an atypical incontinentia pigmenti phenotype. IKBKG mutations are typically associated with preterm male death, but this variant is associated with survival for 8-15 days. PMID:25441681
Recruitment of A20 to the C-terminal domain of NEMO represents a novel mechanism limiting NF-kappaB activation by NEMO, and its absence results in autoinflammatory disease. PMID:26802121
Authors show that Rab11-GMPPNP-FIP3-Rabin8 is more stable than Rab11-GMPPNP-Rabin8, owing to direct interaction between Rabin8 and FIP3 within the dual effector-bound complex. PMID:26258637
Somatic mosaicism of a novel IKBKG nonsense mutation in a male patient with incontinentia pigmenti. PMID:25944529
COMMD7's binding to NEMO does not interfere with the binding to the IKKs, and that the disruption of the IKK complex through the use of the NBP competitor impairs the termination of NF-kappaB activity PMID:26060140
findings suggest that rare, functional variants in MYD88, IRAK4 or IKBKG do not significantly contribute to IPD susceptibility in adults at the population level PMID:25886387
the incontinentia pigmenti patients presented a common IKBKG exon 4-10 deletion PMID:24073555
A novel mutation, designated c.916G>A (p.D306N) is described. NEMO expression was unaffected, but ubiquitylation was decreased causing ectodermal dysplasia, immunodeficiency, incontinentia pigmenti, and immune thrombocytopenic purpura. PMID:26117626
IKKgamma is a parallel coiled-coil whose response to binding of vFLIP or IKKbeta is localized twisting. PMID:25979343
IPO3 binds NEMO, promotes its nuclear import, and is critical for DNA damage-dependent NF-kappaB activation. PMID:26060253
unanchored polyubiquitin has a role in regulation by inducing NEMO conformational change by an allosteric mechanism PMID:25866210
The stability of the NEMO coiled coil is maintained by strong interhelix interactions in the region centered on residue 54. PMID:25400026
Mass spectrometric analysis demonstrated that WA covalently modifies NEMO on a cysteine residue within the C-terminal zinc finger (ZF) domain. Point mutations to the ZF can reverse the WA-induced Lys-48-polyubiquitin binding phenotype PMID:25296760
NEMO patients without ectodermal dysplasia and anhidrosis have more robust immunologic responses. PMID:24682681
the rescuing of the binding affinity implies that a preordered IKK-binding region of NEMO is compatible with IKK binding, and the conformational heterogeneity observed in NEMO(44-111) may be an artifact of the truncation. PMID:25286246
IKBKG gene mutation was discovered as a cause for incontinentia pigmenti. (Meta-analysis) PMID:23802866
We report the results of genomic analysis for a girl with incontinentia pigmenti, but without NEMO mutation PMID:24487970
Data suggest the potential of targeting of Nemo-Like Kinase (NLK) to treat a range of tumourigenic conditions characterised by PTEN deficiency. PMID:23144700
21 new point mutations have been reported, which further extend the spectrum of pathologic variants in Incontinentia pigmenti patients: premature stop codon, frameshift mutation, or a partial loss of NEMO/IKKgamma activity (splicing and missense). PMID:24339369
p62 interacts with NEMO, the regulatory subunit of the complex responsible for activation of NF-kappaB transcription factor. PMID:24270048
NEMO is essential for Kaposi's sarcoma-associated herpesvirus-encoded vFLIP K13-induced gene expression and protection against death receptor-induced cell death. PMID:24672029
Identified is a post-translational modification of NEMO - phosphorylation of residue 387. Phosphorylation of serine 387 is not an absolute requirement for NF-kappaB signalling. PMID:24012789
IKKgamma facilitates RhoA activation via a guanine nucletotide exchange factor, which in turn activates ROCK to phosphorylate IKKbeta, leading to NF-kappaB activation that induced the chemokine expression and cell migration upon TGF-beta1. PMID:24240172
Data suggest that all seven cysteines (4 in zinc finger domain) of NEMO (NF-kappaB essential modulator protein) can be simultaneously mutated to alanine without affecting binding affinity of NEMO for I-kappa B kinase beta catalytic subunit. PMID:24266532
USP10 inhibits genotoxic NF-kappaB activation by MCPIP1-facilitated deubiquitination of NEMO. PMID:24270572
Merkel cell polyomavirus small T antigen targets the NEMO adaptor protein to disrupt inflammatory signaling. PMID:24109239
NEMO ZF, like other NEMO related-ZFs, binds mono-Ub and di-Ub with distinct stoichiometries, indicating the presence of a new Ub site within the NEMO ZF. PMID:24100029