IKBKE Recombinant Monoclonal Antibody

Code: CSB-RA966884A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, WB, IHC, IF, FC

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Target Background

Product Details

Uniprot NO.

Q14164

Target Names

IKBKE

Alternative Names

Inhibitor of nuclear factor kappa-B kinase subunit epsilon (I-kappa-B kinase epsilon) (IKK-E) (IKK-epsilon) (IkBKE) (EC 2.7.11.10) (Inducible I kappa-B kinase) (IKK-i), IKBKE, IKKE IKKI KIAA0151

Species Reactivity

Human

Immunogen

A synthesized peptide from human IKBKE protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

10C6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: HL-60 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), Raji whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), Hela whole cell lysate(30µg), THP-1 whole cell lysate(30µg)
All lanes: IKK epsilon antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 80 kDa
Observed band size: 95 kDa
Exposure time：1min
IHC image of CSB-RA966884A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA966884A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of A549 cell with CSB-RA966884A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing jurkat cells stained with CSB-RA966884A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

IKBKE, also known as IKK-epsilon, serves as a critical non-canonical IκB kinase that plays essential roles in innate immune signaling, interferon regulatory factor activation, and oncogenic transformation. This kinase has garnered significant research attention for its involvement in NF-κB pathway regulation, antiviral responses, and its emerging role as a breast cancer oncogene, making it a compelling target for investigators studying inflammation, immunity, and cancer biology.

This recombinant monoclonal antibody, clone 10C6, offers the reproducibility and consistency that demanding research applications require. Generated through recombinant technology with a defined sequence, this rabbit IgG eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The antibody was raised against a synthetic peptide derived from human IKBKE protein and purified by affinity chromatography to ensure specificity.

Validation across multiple experimental platforms demonstrates this antibody's versatility in your workflow. Western blot analysis successfully detects IKBKE in diverse human cell lines including HL-60, Jurkat, Raji, MCF-7, HeLa, and THP-1, with the observed band appearing at approximately 95 kDa rather than the predicted 80 kDa—a shift likely attributable to post-translational modifications such as phosphorylation or glycosylation common to this kinase. Immunohistochemistry performance has been confirmed in paraffin-embedded human breast cancer and small intestine tissues, while immunofluorescence staining in A549 cells and flow cytometry analysis in Jurkat cells further expand your application options.

Whether investigating innate immune signaling cascades, exploring IKBKE's oncogenic functions in breast cancer, or characterizing inflammatory pathway components, this antibody provides a reliable tool for advancing your research objectives.

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Target Background

Function(From Uniprot)

Serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. Also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling. Following activation of viral RNA sensors, such as RIG-I-like receptors, associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNB. In order to establish such an antiviral state, IKBKE forms several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including IPS1/MAVS, TANK, AZI2/NAP1 or TBKBP1/SINTBAD can be recruited to the IKBKE-containing-complexes. Activated by polyubiquitination in response to TNFA and interleukin-1, regulates the NF-kappa-B signaling pathway through, at least, the phosphorylation of CYLD. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. In addition, is also required for the induction of a subset of ISGs which displays antiviral activity, may be through the phosphorylation of STAT1 at 'Ser-708'. Phosphorylation of STAT1 at 'Ser-708' seems also to promote the assembly and DNA binding of ISGF3 (STAT1:STAT2:IRF9) complexes compared to GAF (STAT1:STAT1) complexes, in this way regulating the balance between type I and type II IFN responses. Protects cells against DNA damage-induced cell death. Also plays an important role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Phosphorylates AKT1.

Gene References into Functions

fascin1 constitutively interacts with IkappaB kinase (IKK) in the RIG-I signaling pathway. In summary, we have identified fascin1 as a suppressor of the RIG-I signaling pathway associating with IkappaB kinase in DLD-1 colon cancer cells to suppress immune responses to viral infection. PMID:29496994
Single-Nucleotide Polymorphism in IKBKE gene had a strong association with resistance or susceptibility to dengue disease. PMID:30332343
These findings indicated that microRNA-98 could promote apoptosis of glioma cells via inhibiting inhibitor of kappa B kinase epsilon/nuclear factor-kappa B signaling and presented a novel regulatory pathway of microRNA-98 by direct suppression of inhibitor of kappa B kinase epsilon/nuclear factor-kappa B expression in glioma cells. PMID:29333957
In conclusion, the spindle cell morphology should be induced by RelA activation (p-RelA S468) by IKKepsilon upregulation in human herpesvirus 8 vFLIP-expressing EA hy926 cells. PMID:30029010
Studied association between nuclear factor kappa beta1/IKK epsilon (NF-kappaB1/IKKepsilon) gene expression and clinical activity in rheumatoid arthritis (RA). Found there were significant differences in NF-kappaB1 and IKKepsilon gene expression (P </= .001 and P </= .029, respectively) between RA patients with clinical activity and clinical remission. PMID:29069487
IKBKE plays a pivotal role in regulating cell proliferation, invasion and epithelial-mesenchymal transition of malignant glioma cells in vitro and in vivo by impacting on the Hippo pathway. PMID:28548934
Inverse association between IKKepsilon and lymph node metastasis in breast cancer. PMID:28532474
YPEL5 silencing enhanced the induction of IFNB1 by pattern recognition receptors and phosphorylation of TBK1/IKBKE kinases, whereas co-immunoprecipitation experiments revealed that YPEL5 interacted physically with IKBKE. PMID:27705791
Virus-activated kinase IKKepsilon phosphorylated YAP at Ser403 and thereby triggered degradation of YAP in lysosomes and, consequently, relief of YAP-mediated inhibition of the cellular antiviral response. PMID:28481329
We then determined that YAP1 negatively regulates miR-let-7b/i by overexpressing and silencing YAP1 expression. In addition, miR-let-7b/i feedback decreases the expression of IKBKE and YAP1 and suppresses the transportation of YAP1 located in the nucleus. Therefore, the regulatory feedback circuit of IKBKE upward arrow-->YAP1 upward arrow-->miR-let-7b/i downward arrow-->IKBKE upward arrow dictates glioblastoma progress... PMID:28677425
our findings highlight the functional importance of IKBKE in pancreatic cancer PMID:28069799
we define a pathobiologic function for IKKepsilon in reprogramming glucose metabolism and driving progression in pancreatic ductal adenocarcinoma PMID:27923829
demonstrate a key role of TBK1/IKKepsilon in the survival and proliferation of HTLV-1-transformed T cells PMID:27123832
Data suggest that changes in inhibitor of nuclear factor kappa B kinase subunit epsilon (IKKepsilon) and TANK-binding kinase 1 (TBK1) expression may be involved in the development of intestinal-type gastric cancer. PMID:27145266
Live NiV infection, but not a recombinant NiV lacking the M protein, reduced the levels of endogenous TRIM6 protein expression. To our knowledge, matrix proteins of paramyxoviruses have never been reported to be involved in innate immune antagonism. We report here a novel mechanism of viral innate immune evasion by targeting TRIM6, IKKepsilon and unanchored polyubiquitin chains. PMID:27622505
These findings collectively support the conclusion that IKK modulates innate immune signaling cascades via phosphorylating the RIG-I cytosolic sensor, providing a feedback regulatory mechanism. PMID:26354181
Blunting of NOX2-dependent IKKepsilon oncogenic kinase expression reduces proliferation and viability of human breast cancer cells. PMID:26177467
The P proteins from the rabies virus street strains 1088 and HCM-9 significantly inhibited I-kappa B kinase (IKK)-inducible IRF-3-dependent IFN-beta promoter activity. PMID:26647356
IKBKE Upregulation is Positively Associated with Squamous Cell Carcinoma of the Lung In Vivo and Malignant Transformation of Human Bronchial Epithelial Cells In Vitro. PMID:26025939
DC-SIGN directs adaptive T helper cell type-2immunity to fucose-expressing pathogens via an IKKepsilon-CYLD-dependent signalling pathway leading to Bcl3 activation. PMID:24867235
these findings reveal that IKBKE-associated cytokine signaling promotes tumorigenicity of immune-driven TNBC and identify a potential therapeutic strategy using clinically available compounds. PMID:25365225
A role for APPL1 in TLR3/4-dependent TBK1 and IKKepsilon activation in macrophages. PMID:25780039
The results highlight the importance of the C-terminal region in the functional activity of IKKepsilon in innate immune response.Interestingly, corresponding region and residues are not required for activation of downstream signaling by TBK1. PMID:24722368
The above-mentioned data suggest that let-7b/i inhibit the invasive ability of glioma cells by directly downregulating IKBKE and indirectly upregulating E-cadherin. PMID:25656572
IKBKE is a novel regulator that limits chronic inflammation during metabolic disease and atherosclerosis. The pathogenic relevance of IKBKE was indicated by the colocalization with macrophages in tissues and in atherosclerotic plaques. PMID:25540417
HTLV-1 Tax is recruited into the TBK1/IKKvarepsilon complexes as a scaffolding-adaptor protein that enhances IFN-beta gene expression. PMID:25531185
Authors identified polymorphisms in the IkBepsilon gene associated with spontaneous hepatitis C resolution in two independent cohorts. PMID:24224717
in this work, we identified TPCA-1 as a direct dual inhibitor for both IKKs and STAT3, whereas treatment targeting EGFR only could not sufficiently repress NF-kappaB and STAT3 pathways for lung cancers harboring mutant EGFR. PMID:24401319
probe F2 facilitated the identification of the target spectrum of the two inhibitors confirming many of the previously identified (off-) targets such as AURKA, FLT4-VEGFR3, IKBKE and PDGFRbeta. PMID:24184958
Using interaction assays, authors found that dengue virus NS2B/3 interacts with the cellular IkappaB kinase epsilon (IKKepsilon). PMID:24173023
Through interaction with TBK1, phlebovirus nonstructural protein sequester the IKKepsilon and IRF3 into viral inclusion bodies. PMID:24335286
IKK-epsilon phosphorylated the transcription factor IFN regulatory factor 1 (IRF-1). PMID:24396068
study provides convinced morphologic evidence that VMAT2 is not present in beta cells PMID:24155403
we summarize the roles of IKKepsilon and TBK1 in different diseases and outline therapeutic options for modulation of these kinases PMID:23157677
IKKepsilon increases ERalpha-36 expression and is involved in ERalpha-36 mediated non-genomic estrogen signaling. PMID:23816933
Ten IKBKE SNVs were associated with systemic lupus erythematosus. PMID:23535865
IKBKE regulates FOXO3a primarily through phosphorylation of SerS644 PMID:23691078
Sp1 is required for IL-15 induction by both poly(I:C) and respiratory syncytial virus, a response that also requires NFkappaB2 and IKKepsilon. PMID:23873932
DDX3 is a scaffolding adaptor that directly facilitates phosphorylation of IRF3 by IKK-epsilon. PMID:23478265
IkappaBepsilon may be an effective target for HIV latency reactivation in T-cell and macrophage lineages. PMID:23365428
These experiments demonstrated direct physical interactions of the IKKepsilon and TBK1 kinases with hepatitis C virus NS2. PMID:23096996
IKBKE is a direct target of STAT3 and is induced by tobacco carcinogens through STAT3 pathway. PMID:22330135
This work defines TRAF2 phosphorylation to be one key effector of IKKE-induced mammary epithelial cell transformation. PMID:23007157
these data provide evidence that IKK-epsilon is a key coordinator of invasion and metastasis programs in ovarian cancer PMID:22942254
NP-IKKepsilon interaction likely plays a crucial role in arenavirus-host interaction. PMID:22532683
phosphorylation of IRF7 on Ser477 and Ser479 by IKKepsilon or TBK1 is inhibited by KSHV ORF45 PMID:22787218
deregulation of IKKepsilon plays a pivotal role in the uncontrolled proliferation and malignant invasion of glioma cells PMID:22552702
identify FOXO3 as a new IKK-epsilon-controlled check-point of IRF activation and regulation of IFN-beta expression, providing new insight into the role of FOXO3 in immune response control. PMID:22531926
IKBKE and gene expression patterns for other members of the NF-kappaB pathway were not associated with survival, suggesting that IKBKE gene expression may be an independent marker of variation in overall survival PMID:22266464
Inhibitor of kappaB kinase epsilon (IKK(epsilon)), STAT1, and IFIT2 proteins define novel innate immune effector pathway against West Nile virus infection. PMID:22065572

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Subcellular Location

Cytoplasm. Nucleus. Nucleus, PML body.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, I-kappa-B kinase subfamily

Tissue Specificity

Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.

Database Links

HGNC: 14552

UNIGENE: Hs.321045

KEGG: hsa:9641

STRING: 9606.ENSP00000356087

OMIM: 605048

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