HIF1A Recombinant Monoclonal Antibody

Code: CSB-RA624113MA1HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, IF, FC

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Target Background

Product Details

Uniprot NO.

Q16665

Target Names

HIF1A

Alternative Names

ARNT interacting protein antibody; ARNT-interacting protein antibody; Basic helix loop helix PAS protein MOP1 antibody; Basic-helix-loop-helix-PAS protein MOP1 antibody; bHLHe78 antibody; Class E basic helix-loop-helix protein 78 antibody; HIF 1A antibody; HIF 1alpha antibody; HIF-1-alpha antibody; HIF-1alpha antibody; HIF-alpha antibody; HIF1 A antibody; HIF1 Alpha antibody; HIF1 antibody; HIF1-alpha antibody; HIF1A antibody; HIF1A_HUMAN antibody; hifla antibody; Hypoxia inducible factor 1 alpha antibody; Hypoxia inducible factor 1 alpha isoform I.3 antibody; Hypoxia inducible factor 1 alpha subunit antibody; Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody; Hypoxia inducible factor 1; alpha subunit (basic helix loop helix transcription factor) antibody; Hypoxia inducible factor1alpha antibody; Hypoxia-inducible factor 1-alpha antibody; Member of PAS protein 1 antibody; Member of PAS superfamily 1 antibody; Member of the PAS Superfamily 1 antibody; MOP 1 antibody; MOP1 antibody; PAS domain-containing protein 8 antibody; PASD 8 antibody; PASD8 antibody

Species Reactivity

Human

Immunogen

Recombinant Human HIF1A protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8C8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
IF	1:20-1:200
FC	1:20-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Immunofluorescence staining of Hela cell with CSB-RA624113MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing Hela cells stained with CSB-RA624113MA1HU (red line) at 1:50. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was rabbit IgG1 (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,001 events was performed.

Description

Hypoxia-inducible factor 1-alpha (HIF1A) serves as a master regulator of cellular responses to low oxygen conditions, orchestrating transcriptional programs that influence angiogenesis, metabolic adaptation, and cell survival. As a critical component of the HIF-1 heterodimeric transcription factor, HIF1A drives the expression of genes essential for tumor progression, cardiovascular remodeling, and metabolic reprogramming, making it an indispensable target for researchers investigating oxygen-sensing pathways and disease mechanisms.

This recombinant monoclonal antibody, clone 8C8, offers the reproducibility and consistency that demanding experimental workflows require. Developed using recombinant technology with a defined sequence, this rabbit IgG antibody eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. Affinity-purified to ensure specificity, it provides reliable detection of human HIF1A across multiple experimental platforms.

Validation studies demonstrate robust performance in both immunofluorescence and flow cytometry applications. Immunofluorescence staining of HeLa cells at dilutions ranging from 1:20 to 1:200 reveals clear cellular localization patterns when counter-stained with DAPI, enabling visualization of HIF1A distribution within fixed samples. Flow cytometry analysis of permeabilized HeLa cells confirms specific detection with clear separation from isotype control, supporting quantitative assessment of HIF1A expression at the single-cell level.

Whether investigating hypoxic tumor microenvironments, exploring metabolic stress responses, or examining cardiovascular adaptation mechanisms, this antibody provides the flexibility to transition between imaging and cytometric analyses using a single, well-characterized reagent. Its validated performance in human cell models positions it as a dependable tool for epigenetics, cancer biology, cardiovascular research, and metabolic studies.

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Target Background

Function(From Uniprot)

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with NCOA1 and/or NCOA2. Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.; (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, is required for induction of glycolysis in monocytes and the consequent proinflammatory state. In monocytes, induces expression of ACE2 and cytokines such as IL1B, TNF, IL6, and interferons. Promotes human coronavirus SARS-CoV-2 replication and monocyte inflammatory response.

Gene References into Functions

These findings demonstrated a valuable antitumor synergism in combining CRISPR/Cas9mediated HIF1alpha knockout with Transarterial embolization (TAE)in mice and highlighted the possibility that HIF1a may be an effective therapeutic knockout target in combination with TAE for hepatocellular carcinoma treatment PMID:30226584
Since the FIH-1 dependent hydroxylation of NAA10 occurs oxygen-dependently, NAA10 acetylates HIF-1alpha under normoxia but does not under hypoxia. PMID:30237125
HIF1A is upregulated in breast and bladder tumors with high NRF2 activity. NRF2 targets a functional antioxidant response element at the HIF1A locus, which reveals a direct regulatory connection between two important oxygen responsive transcription factors. PMID:30241031
CPT1A is repressed by HIF1 and HIF2, reducing fatty acid transport into the mitochondria, and forcing fatty acids to lipid droplets for storage. PMID:29176561
MiR-497 approximately 195 cluster regulates angiogenesis during coupling with osteogenesis by maintaining endothelial Notch1 and HIF1A activity. PMID:28685750
Study revealed that HIF1alpha was significantly upregulated in gallbladder cancer (GBC) tissues. HIF1alpha overexpression was closely associated with lymph node metastasis and TNM stage. HIF1alpha was able to promote cell migration in a hypoxic microenvironment by overexpressing VEGF in GBC cell line. PMID:30272364
HIF-1alpha "positive" had decreased overall survival compared to HIF-1alpha "negative" patients and this was an independent adverse prognostic factor for HCC patients with cirrhosis, but not for cirrhosis-free patients. PMID:30274686
genetic polymorphisms in HIF1A were not associated with persistent apical periodontitis PMID:29898065
Hypoxia-induced angiogenesis is a complex process that involves distinct but also overlapping functions of HIF-1alpha and HIF- 2alpha in regard to angiogenesis, bioenergetic adaption and the redundant transcriptional induction of MIF. PMID:28993199
High HIF1A expression is associated with high Collagen I Fibers in Triple Negative Breast Cancer. PMID:29247885
Cathepsin B (CTSB) is a novel target gene of hypoxia-inducible factor-1-alpha (HIF-1alpha). CTSB mRNA and protein levels can be up-regulated in a HIF-1alpha-dependent manner. PMID:29935187
this is the first case-control study uncovering that the HIF1A gene rs10873142 polymorphism increases the risk of COPD in a Chinese Han population. PMID:29339421
ST6Gal-I activity augmented HIF-1alpha accumulation in cells grown in a hypoxic environment or treated with two chemical hypoxia mimetics, deferoxamine and dimethyloxalylglycine. PMID:29475939
mRNA expression levels of both HIF1A and LACC1 were upregulated in the skin lesions of individuals with leprosy. PMID:29706348
data suggest an important role of miR-210 in sustaining HIF-1alpha activity via the suppression of HIF-3alpha, regulating cell growth and chemotherapeutic drug resistance in cholangiocarcinoma. PMID:29953500
HOXA9 inhibits HIF1A-mediated glycolysis through interacting with CRIP2 to repress cutaneous squamous cell carcinoma development. PMID:29662084
HIF-1alpha inhibits NCR1/NKp46 pathway through up-regulating miR-224, which affects the killing capability of NK cells on prostate cancer, thus inducing immune escape of tumor cells. PMID:29885835
The findings demonstrate that AGPAT2, which is mutated in patients with congenital generalized lipodystrophy and over-expressed in different types of cancer, is a direct transcriptional target of HIF-1, suggesting that upregulation of lipid storage by HIF-1 plays an important role in adaptation and survival of cancer cells under low oxygen conditions. PMID:29908837
Overexpression of HIF1A leads to radioresistance of cervical cancer. PMID:30355300
Knockdown of DEC2 resulted in a significant (26.7%) reduction of VEGF expression in MIO-M1 cells under hypoxia-mimicking conditions induced by DFO (P < .05). Levels of HIF1alpha protein were also reduced significantly, by 60.2%, in MIO-M1 cells treated with siRNA against the DEC2 gene (P < .05). Moreover, HIF1alpha levels showed a significant (2.5-fold) increase in MIO-M1 cells overexpressing DEC2 (P < .05). PMID:30250985
High HIF1A expression is associated with salivary adenoid cystic carcinoma. PMID:30015895
we found that Hif-1alpha directly promoted H19 expression through binding to the H19 promoter and indirectly through SP1-mediated H19 transcriptional activation under hypoxia in glioblastoma cells. PMID:28327666
The IRIS-driven metastatic mechanism results from IRIS-dependent suppression of phosphatase and tensin homolog (PTEN) transcription, which in turn perturbs the PI3K/AKT/GSK-3beta pathway leading to prolyl hydroxylase-independent HIF-1alpha stabilization and activation in a normoxic environment. PMID:30254159
Data show that von Hippel-Lindau-binding protein 1 (VBP1) enhances the stability of von Hippel-Lindau tumor suppressor protein (pVHL) and facilitates pVHL-mediated ubiquitination of hypoxia-inducible factor 1, alpha subunit (HIF-1alpha). PMID:29121446
Multifunctional proteins epigenetically modulating HIF1A stability and activity have been described. (Review) PMID:29032501
Studies have shown that both HIF1alpha and HIF2alpha may contribute to the regulation of cellular adaptation to hypoxia and resistance to cancer therapies with their potential to exert significant effects on the maintenance and evolution of cancer stem cells. Also, HIF1alpha and HIF2alpha seemed to have significant prognostic and predictive value. [review] PMID:29845228
HIF-1 was overexpressed in osteosarcoma tissues and cell lines, which promoted cell proliferation, clone formation, migration, invasion and inhibited cell apoptosis. PMID:29807229
hypoxiainduced expression of CXCR4 promoted trophoblast cell migration and invasion via the activation of HIF1alpha, which is crucial during placentation. PMID:29786753
Data suggest that NRF2/NFE2L2 promotes breast cancer progression by enhancing glycolysis through co-activation of HIF1A; NRF2 and HIF1A mRNA and protein levels are significantly up-regulated in breast cancer cells as compared to benign breast epithelial cells. (NRF2/NFE2L2 = nuclear factor erythroid 2-related factor 2; HIF1A = hypoxia inducible factor 1 subunit alpha) PMID:29275212
the expression of MMIF, HIF-1alpha, and VEGF in the serum and endometrial tissues may be used to assess the stage of endometriosis and the severity of dysmenorrhea. PMID:30074218
Findings revealed an HIF-1alpha/IL-1beta signaling loop between cancer cells and tumor-associated macrophages in a hypoxic microenvironment, resulting in cancer cell epithelial-mesenchymal transition and metastasis; more importantly, our results suggest a potential role of an anti-inflammatory strategy in hepatocellular carcinoma treatment PMID:29171040
Overexpression of HIF-1alpha and P4HB is associated with poor prognosis in patients with gastric cancer. PMID:29904245
hypoxic stress in the hepatocellular carcinoma (HCC)cells promoted YAP binding to HIF-1a in the nucleus and sustained HIF-1a protein stability to bind to PKM2 gene and directly activates PKM2 transcription to accelerate glycolysis PMID:30180863
Overexpression of VHL was more successful at inhibiting fibrosis compared with silencing HIF-1a plus HIF-2a. Normoxia-active HIF-1a or HIF-2a prevented the inhibitory effect of VHL on liver fibrosis, indicating that attenuating fibrosis via VHL is HIF-1a- and HIF-2a-dependent to some extent. PMID:28112200
Results find that knockdown of HIF-1alpha reduced hypoxia-induced SENP1 expression, suggesting that induction of SENP1 expression is mediated by hypoxia-inducible factor HIF-1alpha. PMID:28796315
ADM was an upstream molecule of HIF-1alpha/VEGF and it promoted angiogenesis through upregulating HIF-1alpha/VEGF in epithelial ovarian cancer. PMID:28091613
HIF-1alpha expression correlates with the expression level of IL- 8, as evidenced by the down regulation of IL-8 in response to silencing of HIF-1alpha in HCC cell lines under hypoxic conditions. PMID:29881400
MOLP8/R cells display a very high overexpression of LCP1 gene (l-Plastin) controlled by HIF1&alpha. PMID:29882856
the role of FTH1 in the FIH control of HIF-1 activity, is reported. PMID:29580991
The findings of the current study demonstrate presence of the IDH1 R132H mutation in primary human glioblastoma cell lines with upregulated HIF-1alpha expression, downregulating c-MYC activity and resulting in a consequential decrease in miR-20a, which is responsible for cell proliferation and resistance to standard temozolomide treatment. PMID:29625108
The cross-talk between HIF1A, NRF2 and NF-kappaB is required to adapt to changes in oxygen availability. (Review) PMID:29485192
PKD1 not only regulates the hypoxic glycolytic metabolism of cancer cells via regulation of the expression of HIF-1alpha and glycolytic enzymes. PMID:29901206
Results provide evidence that HIF1alpha overexpression promotes the radioresistance of prostate cancer cells through enhanced betacatenin nuclear translocation. PMID:29658569
Collectively, the present study demonstrated mitochondrial fission as a tumor suppression process that is regulated by the HIF/miR125a/Mfn2 pathways, acting to restrict PANC1 cell survival, energy metabolism and migration, with potential implications for novel approaches for PC therapy. PMID:29749475
the results of the present study demonstrated that hypoxia-induced cytoprotective autophagy counteracted gemcitabine-induced apoptosis through increasing HIF1alpha expression PMID:29693166
CD40 is a key molecule for the upregulation of HIF-1alpha and PTEN underlying the severity of microangiopathy. PMID:29549140
Enhanced expression of HIF-1alpha may be related to autophagy activation in SH-SY5Y cells, thus contributing to ischemic/hypoxic brain damage. PMID:29724989
Data show that deletion of hypoxia inducible factor 1 subunit alpha (HIF-1alpha) in NK cells inhibited tumour growth despite impaired tumour cell killing. PMID:29150606
AEG-1 was found to be significantly associated with hypoxia in ovarian cancer by regulating the HIF-1alpha/NF-kappaB/VEGF pathway. PMID:29770329
no significant differences in the serum levels between early-onset pre-eclampsia, late-onset pre-eclampsia, and controls PMID:28574293

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Subcellular Location

Cytoplasm. Nucleus. Nucleus speckle.

Tissue Specificity

Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumo

Database Links

HGNC: 4910

UNIGENE: Hs.597216

KEGG: hsa:3091

STRING: 9606.ENSP00000338018

OMIM: 603348

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