H2AFY Recombinant Monoclonal Antibody

Code: CSB-RA135386A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human, Mouse, Rat

Application: ELISA, WB, IHC, IF, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

O75367

Target Names

H2AFY

Alternative Names

Core histone macro h2a.1 antibody; Core histone macro-H2A.1 antibody; H2A histone family member Y antibody; H2A.y antibody; H2A/y antibody; H2AF12M antibody; H2AFJ antibody; H2afy antibody; H2AY_HUMAN antibody; Histone H2A.Y antibody; Histone macroH2A1 antibody; Histone macroH2A1.1 antibody; Histone macroH2A1.2 antibody; Macroh2a1 antibody; MACROH2A1.1 antibody; MacroH2A1.2 antibody; Medulloblastoma antigen MU MB 50.205 antibody; Medulloblastoma antigen MU-MB-50.205 antibody; mH2a antibody; mH2A1 antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human H2AFY protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

17G2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Hela whole cell lysate(30µg), HepG2 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), COLO-205 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg)
All lanes: Histone mH2A1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time：10s
IHC image of CSB-RA135386A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA135386A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of A431 cell with CSB-RA135386A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing THP-1 cells stained with CSB-RA135386A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Description

MacroH2A1, encoded by the H2AFY gene, represents a distinctive histone H2A variant that plays a central role in epigenetic regulation and chromatin organization. This variant incorporates a unique macro domain that extends beyond the nucleosome core, enabling specialized functions in X chromosome inactivation, gene silencing, and cellular differentiation. Its involvement in these fundamental processes makes MacroH2A1 a compelling target for researchers investigating epigenetic mechanisms, developmental biology, and cancer progression.

This recombinant monoclonal antibody, clone 17G2, offers the reproducibility and consistency that demanding epigenetic studies require. Generated against a synthetic peptide derived from human H2AFY protein, the recombinant format ensures sequence-defined specificity and eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG isotype combined with affinity chromatography purification delivers reliable performance across experimental platforms.

Validation data demonstrates robust versatility across multiple applications. Western blot analysis confirms detection of the expected 40 kDa band across diverse human cell lines including HeLa, HepG2, THP-1, SH-SY5Y, MCF-7, COLO-205, and HEK293, with cross-species reactivity confirmed in mouse and rat brain tissue lysates. Immunohistochemistry staining has been validated in human testis and breast cancer tissue sections, while immunofluorescence studies in A431 cells reveal clear nuclear localization patterns consistent with MacroH2A1's chromatin-associated function. Flow cytometry analysis in THP-1 cells further expands the application range for single-cell studies.

Whether investigating chromatin dynamics, exploring epigenetic alterations in disease models, or characterizing histone variant distribution across tissues, this antibody provides a dependable tool for advancing your research in epigenetics and nuclear signaling.

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Target Background

Function(From Uniprot)

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin.; Binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation. Increases the expression of genes involved in redox metabolism, including SOD3.; Represses SOD3 gene expression.

Gene References into Functions

This article points to a splicing-regulated, proto-oncogenic role for the macroH2A1.2 variant and suggests manipulation of macroH2A1.2 expression as a potential therapeutic means to interfere with tumorigenesis. PMID:29249653
The Skp2-mH2A1-CDK8 axis has a critical role in breast cancer development via dysregulation of the G2/M transition, polyploidy, cell growth dysregulation, and loss of tumor suppression. PMID:25818643
We show that composite nucleosomes containing mH2A and NRF-1 are stably positioned on gene regulatory regions and can buffer transcriptional noise associated with antiviral responses PMID:25959814
High MacroH2A1 expression is associated with epigenetic markers for activation of lipogenic genes in fat-induced steatosis. PMID:25526730
macroH2A1.1 expression correlates with poor survival of triple-negative breast cancer patients PMID:24911873
MacroH2A1.1 and PARP-1 cooperate to regulate transcription by promoting CBP-mediated H2B acetylation. PMID:25306110
MacroH2A1 specifically recruits PELP1 to the promoters of macroH2A1 target genes, but macroH2A1 occupancy occurs independent of PELP1. This recruitment allows macroH2A1 and PELP1 to cooperatively regulate gene expression outcomes. PMID:24752897
The results demonstrate that macroH2A1 is a new factor involved in the regulation of rDNA transcription. PMID:24071584
macro histone variants (macroH2A) are expressed at low levels in stem cells and are up-regulated during differentiation PMID:23595991
Both macroH2A1 isoforms may play a role in hepatocellular carcinoma pathogenesis and may be considered as novel diagnostic markers for human hepatocellular carcinoma. PMID:23372727
MacroH2A1 splicing isoforms differentially regulate the transcription of a set of genes involved in redox metabolism. PMID:23022728
The histone variant macroH2A1.1 is recruited to DNA double-strand breaks through a mechanism involving PARP1. PMID:23031826
Within the macro domain of mH2A1.2, a trinucleotide insertion (-EIS-) sequence not found in mH2A1.1 was essential for the interaction between HER-2 and mH2A1.2 as well as mH2A1.2-induced HER-2 expression and cell proliferation. PMID:22589551
ATRX (alpha-thalassemia/MR, X-linked) is a novel macroH2A-interacting protein PMID:22391447
H2AFY is specifically overexpressed in the blood and frontal cortex of patients with Huntington disease compared with controls PMID:21969577
macroH2A can play either a positive or negative role in transcriptional regulation in a context-dependent manner. Additionally, macroH2A has been linked to the control of the cell cycle and cell proliferation. PMID:20543561
significant inverse correlation between mH2A and CDK8 expression levels exists in melanoma patient samples PMID:21179167
distributed during the maintainance phase of x inactivation throughout cell cycle PMID:12082075
MACROH2A1 deposition is regulated by the CULLIN3/SPOP ligase complex and is actively involved in stable X inactivation. PMID:15897469
A specific interaction between mH2A1.1 and PARP-1 was demonstrated and found to be associated with inactivation of PARP-1 enzymatic activity. PMID:17158748
multiple short sequences dispersed along the macroH2A1 histone domain individually supported enrichment on the inactive X chromosome when introduced into H2A PMID:17570398
A phosphorylation site, S137ph, was identified in mH2A1;S137ph is enriched in mitosis. PMID:18227505
expression of histone macroH2A1.1 and macroH2A2 predicts lung cancer recurrence. PMID:19648962
show that distinct macrodomains, including those of histone macroH2A1.1, are recruited to sites of PARP1 activation induced by laser-generated DNA damage. PMID:19680243
an unexpected role for macroH2A1 in the escape from heterochromatin-associated silencing and the enhancement of autosomal gene transcription PMID:20008927