GPRC5D Recombinant Monoclonal Antibody

Code: CSB-RA882153MA01HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA

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Target Background

Product Details

Uniprot NO.

Q9NZD1

Target Names

GPRC5D

Alternative Names

GPRC5D; G-protein coupled receptor family C group 5 member D

Species Reactivity

Human

Immunogen

Recombinant Human GPRC5D protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG4(S228P)

Clone No.

3D4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

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The Binding Activity of Human GPRC5D with Anti-GPRC5D recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human GPRC5D(CSB-MP882153HU) at 5 μg/mL can bind Anti-GPRC5D recombinant antibody, the EC₅₀ is 8.847-10.38 ng/mL.

Description

The creation of the GPRC5D recombinant monoclonal antibody follows a meticulous and precise process to ensure its exceptional quality and specificity. It begins with the isolation of B cells from an immunized animal, where the recombinant human GPRC5D protein is used as the immunogen. Total RNA is extracted from these B cells and converted into cDNA through reverse transcription. The GPRC5D antibody genes are then amplified using specific primers designed for the antibody constant regions and inserted into an expression vector. Through transfection, the vector is introduced into host cells, enabling the production of the GPRC5D recombinant monoclonal antibody. Following a period of cell culture, the antibody is harvested from the supernatant and subjected to purification using affinity chromatography, resulting in a highly purified form suitable for diverse applications. CUSABIO performs ELISA to validate the antibody's specificity and functionality in detecting human GPRC5D protein.

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Target Background

Gene References into Functions

GPRC5D in MM cells makes this gene and its encoded surface protein as promising markers for monitoring the tumor load and hopefully also as targets for antimyeloma antibodies. PMID:23510526
Overexpression of G protein-coupled receptor 5D in the bone marrow is associated with poor prognosis in patients with multiple myeloma. PMID:22591013

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 3 family

Tissue Specificity

Widely expressed in the peripheral system. Expression pattern is high in pancreas, medium in kidney, small intestine, spleen and testis, low in lung, colon, leukocyte, prostate and thymus and not detectable in brain, heart, liver, placenta, skeletal muscl

Database Links

HGNC: 13310

UNIGENE: Hs.644599

KEGG: hsa:55507

STRING: 9606.ENSP00000228887

OMIM: 607437

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