GPNMB Recombinant Monoclonal Antibody

Code: CSB-RA622928MA1HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, FC

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Target Background

Product Details

Uniprot NO.

Q14956

Target Names

GPNMB

Alternative Names

Glycoprotein (transmembrane) nmb antibody; Glycoprotein nmb antibody; Glycoprotein nmb like protein antibody; Gpnmb antibody; GPNMB_HUMAN antibody; HGFIN antibody; NMB antibody; Osteoactivin antibody; Transmembrane glycoprotein antibody; Transmembrane glycoprotein HGFIN antibody; Transmembrane glycoprotein NMB antibody

Species Reactivity

Human

Immunogen

Recombinant Human GPNMB protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Mouse IgG

Clone No.

3G2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
FC	1:20-1:500

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Overlay Peak curve showing Hela cells stained with CSB-RA622928MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10, 003 events was performed.

Description

GPNMB, also known as osteoactivin or glycoprotein NMB, is a type I transmembrane glycoprotein that has emerged as a significant target in both cancer biology and immunology research. This multifunctional protein plays diverse roles in cellular processes including melanocyte differentiation, osteoblast function, and tissue repair. In the tumor microenvironment, GPNMB expression has been associated with disease progression across multiple cancer types, making it an attractive target for both diagnostic investigation and therapeutic development, including antibody-drug conjugate strategies.

This recombinant monoclonal antibody, clone 3G2, offers researchers the reproducibility essential for longitudinal studies and multi-site collaborations. Because the antibody sequence is defined and production occurs through recombinant expression rather than traditional hybridoma methods, you can expect consistent performance across lots, eliminating a common source of experimental variability that can complicate data interpretation.

The antibody has been validated for flow cytometry applications, with testing demonstrated in HeLa cells. In these experiments using fixed and permeabilized cells, the antibody produced clear signal separation from isotype control at a 1:100 dilution, confirming specific detection of intracellular GPNMB. The recommended working range of 1:20 to 1:500 for flow cytometry provides flexibility to optimize signal intensity based on your specific experimental conditions and cell types. ELISA compatibility further extends the utility of this reagent for quantitative protein detection workflows.

For researchers investigating GPNMB biology in cancer progression, immune cell function, or as a potential therapeutic target, this antibody provides a reliable tool for characterizing human GPNMB expression at the single-cell level.

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Target Background

Function(From Uniprot)

Could be a melanogenic enzyme.

Gene References into Functions

loss of GPNMB, which has been implicated in melanosome formation, autophagy, phagocytosis, tissue repair, and negative regulation of inflammation, underlies autosomal-recessive Amyloidosis cutis dyschromica. PMID:29336782
Study shows high GPNMB mRNA level in osteosarcoma tissues and cell lines. Its silencing regulates the proliferation and metastasis of osteosarcoma cells by suppressing the PI3K/Akt/mTOR signaling pathway. PMID:29620278
Serum levels of GPNMB were highly correlated with accumulation of bioactive lipid substrate of Gaucher disease, glucosylsphingosine as well as established biomarkers, chitotriosidase and chemokine, CCL18. PMID:28003098
Increased brain expression of GPNMB is associated with genome wide significant risk for Parkinson's disease PMID:28391543
This study revealed that GPNMB aggregates are localized to the neuron, and not localized to the astrocyte and microglia and in the spinal cord of ALS patients. GPNMB has protective effects against mutant TDP-43-induced motor neuron cell death, via ERK1/2 and Akt pathway.. PMID:27935101
This review will summarize the latest understanding of Gpnmb in the aspects of diagnosis, progression and prognosis of pathological disorders and neoplasms, emphasizing the clinical advances in targeting Gpnmb-expressing malignancies--{REVIEW} PMID:29441921
The combination of MAPK pathway inhibitors with an antibody-drug conjugate targeting GPNMB is an effective therapeutic option. PMID:27515299
High GPNMB expression is associated with enhancement of invasive behavior in lung cancer. PMID:26883195
Findings suggest that MAFK and its target gene GPNMB play important roles in the malignant progression of triple-negative breast cancer (TNBC) cells, offering potentially new therapeutic targets for TNBC patients. PMID:28400538
glycoprotein non-metastatic B presented increased expression in breast cancer in vivo compared to normal breast tissue PMID:28689015
Osteoactivin differential regulates the expression of matrix metalloproteases in head and neck squamous cell carcinomas to promote cancer cell invasion. PMID:28295306
The GPNMB is expressed in a temporal manner during eosinophil development and delivers a proliferative signal upon activation. PMID:28104809
it may be premature to conclude associations between GPNMB rs156429 and Parkinson's disease, amyotrophic lateral sclerosis and multiple system atrophy. PMID:27132081
Glycoprotein nonmetastatic melanoma protein B protein was highly expressed in the bladder cancer, which was related to the poor prognosis in bladder cancer patients. Glycoprotein nonmetastatic melanoma protein B promoted the proliferation, migration, and invasion in bladder cancer cells. PMID:28443476
an inducer for glioma and could enhance matrix metalloproteinase activity through Wnt/beta-catenin pathway to contribute to glioma tumorigenesis PMID:27334625
findings indicate that GPNMB promotes glioma growth via Na(+)/K(+)-ATPase alpha subunits. Thus, the interaction between GPNMB and Na(+), K(+)-ATPase alpha subunits represents a novel therapeutic target for the treatment of brain glioblastomas. PMID:27836549
Finally, we identify glycoprotein NMB as a melanocytic marker up-regulated in Tsc2-null mouse uteri and human lymphangioleiomyomatosis samples PMID:26880751
The GPNMB is a promising biomarker and therapeutic target for the development and progression of Nonalcoholic fatty liver disease in obesity. PMID:26581806
Osteoactivin in the extracellular matrix promotes oral squamous carcinoma cell adhesion and migration. PMID:26636434
Gpnmb Is a Potential Marker for the Visceral Pathology in Niemann-Pick Type C Disease PMID:26771826
GPNMB might be a surrogate marker for BC and may cross talk with the HER2 signal pathway. PMID:26077887
GPNMB plays an important roles in regulating the expression of key pluripotency genes in dental pulp cells and modifying odontogenic differentiation. PMID:26261527
A positive correlation between GPNMB and NRP-1 levels in human breast tumors. PMID:25772243
Data shows that the expression of Gpnmb and Spp1 is highly upregulated in glioma-associated microglia/macrophages highlighting the importance of macrophages and microglia as therapeutic targets in anti-tumor treatment regimens. PMID:25658639
GPNMB/OA protein expression prevents cells from apoptosis-enhancing proliferation and represents a novel modulator of the invasion and metastasis in pancreatic cancer cells. PMID:25426614
The transcription factor MITF is a critical regulator of GPNMB expression in dendritic cells. PMID:25889792
GPNMB mRNA in FLCN-related renal cell carcinomas was 23-fold more abundant than in sporadic tumors. PMID:25594584
GPNMB has protective effect against ischemia-reperfusion injury via phosphorylation of ERK1/2 and Akt PMID:25010402
Glycoprotein nonmetastatic melanoma protein B plays an important role in angiogenesis during hyperoxia injury PMID:25054912
DC-HIL+ CD14+ HLA-DR no/low cells are a potential blood marker and therapeutic target for melanoma PMID:24933321
Our data identify Gpnmb as a novel marker for obesity-induced adipose tissue macrophage infiltration and potentiator of interleukin-4 responses and point toward a crucial role for MITF in driving part of the adipose tissue macrophage phenotype. PMID:24789918
GPNMB/OA acts as a critical molecular mediator promoting the acquisition of the more aggressive, pro-metastatic phenotype distinctive of human DU145 and PC3 cell lines. PMID:24589892
our findings will clarify a new explanation about how GPNMB induces bone repair, and provide a potential target for bone regeneration therapeutics and bone engineering PMID:23794283
the molecular basis for the distinct trafficking and morphogenetic properties of PMEL and GPNMB is the PKD domain PMID:23452376
GPNMB expression was regulated by EpCAM and CSF-1 partly through their common downstream product c-myc PMID:23924854
Silencing of GPNMB by siRNA inhibits the formation of melanosomes in melanocytes in a MITF-independent fashion. PMID:22912767
GPNMB inhibits motor neuron death and plays a critical role in motor neuron survival. PMID:22891158
Together these results suggest that GPNMB gene is a p53- and androgen-dysregulated gene and should be regarded as an anti-tumor gene for prostate cancer. PMID:22290289
The abnormal expression of GPNMB may play an important role in the development of prostate cancer. PMID:21844952
Gastrointestinal stromal tumours do not show immunopositivity for ERa or HMB45. PMID:22014058
upregulated in monocyte-derived dendritic cells by BCR-ABL tyrosine kinase inhibitors PMID:21874302
GPNMB increase in kidney disease was confirmed by real-time PCR after 5/6 nephrectomy, in streptozotocin-induced diabetes, and in patients with chronic kidney disease PMID:21389974
Toxin-conjugated DC-HIL to abrogate the ability of Sezary syndrome cells to proliferate in vitro. PMID:21252093
ADAM10 as a sheddase capable of releasing the GPNMB/OA ectodomain from the surface of breast cancer cells PMID:20711474
GPNMB expression has a role in uveal melanoma PMID:20375921
GPNMB expression is associated with the basal/triple-negative subtype and is a prognostic marker of poor outcome in patients with breast cancer. PMID:20215530
GPNMB is a melanosomal protein that is released by proteolytic ectodomain shedding and might be a useful and specific histological marker of melanocytic cells. PMID:20056711
Human OA uses the same transcriptional initiation site in both bone and kidney as was reported for melanoma cells and is expressed in osteoblast cultures at all stages of differentiation PMID:14696968
cloning and analysis of two fragments in the 5' flanking region of HGFIN; studies indicate p53 cooperates with cytokine-mediated transcription factors to regulate the expression of HGFIN PMID:15684612
Osteoactivin is expressed at high levels in normal and inflammatory liver macrophages suggesting a significant role in acute liver injury. PMID:15763343

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Melanosome membrane; Single-pass type I membrane protein. Early endosome membrane; Single-pass type I membrane protein.

Protein Families

PMEL/NMB family

Tissue Specificity

Widely expressed, but very low expression, if any, in the brain. Expressed in the epidermis with higher levels in melanocytes compared with keratinocytes and Langerhans cells (at protein level). Expressed in peripheral blood, but not bone marrow mononucle

Database Links

HGNC: 4462

UNIGENE: Hs.190495

KEGG: hsa:10457

STRING: 9606.ENSP00000371420

OMIM: 604368

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