GABARAP Recombinant Monoclonal Antibody

Code: CSB-RA546399A0HU

Rate （N/A）
Review （0）

Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human, Mouse, Rat

Application: ELISA, WB, IHC, FC

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

O95166

Target Names

GABARAP

Alternative Names

Gamma-aminobutyric acid receptor-associated protein (GABA(A) receptor-associated protein) (MM46), GABARAP, FLC3B

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human GABARAP protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3C1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: U-251MG whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), THP-1 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), A375 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg)
All lanes: GABARAP antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa
Exposure time：2min
IHC image of CSB-RA546399A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing PC-3 cells stained with CSB-RA546399A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

GABARAP (gamma-aminobutyric acid receptor-associated protein) serves as a critical mediator in autophagy and intracellular trafficking, functioning as an ATG8 family member that facilitates autophagosome-lysosome fusion and GABA(A) receptor clustering at synaptic membranes. Its dual roles in neuronal signaling and cellular quality control mechanisms make GABARAP a valuable target for researchers investigating neurodegenerative diseases, cancer biology, and autophagy-related pathways.

This recombinant monoclonal antibody, generated from clone 3C1 in rabbit host, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures comparable results across experiments.

Validation across multiple platforms demonstrates this antibody's versatility in diverse experimental contexts. Western blot analysis confirms reliable detection in human cell lines including U-251MG, SH-SY5Y, THP-1, HEK293, MCF-7, PC-3, and A375, with cross-species reactivity validated in mouse and rat brain tissue lysates. The observed band at approximately 17 kDa, slightly higher than the predicted 14 kDa molecular weight, likely reflects post-translational modifications such as lipidation that occur during GABARAP's conjugation to autophagosomal membranes. Immunohistochemistry validation in human ovarian cancer tissue and flow cytometry analysis in PC-3 cells further expand the application range, providing researchers flexibility to examine GABARAP expression from tissue architecture to single-cell resolution.

Whether investigating autophagy flux, neurotransmitter receptor trafficking, or tumor biology, this antibody supports research spanning neuroscience, oncology, and cell biology applications where precise GABARAP detection is essential.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in autophagy: while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover. Also required for the local activition of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction. Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation. Involved in apoptosis.

Gene References into Functions

The centrosome and centriolar satellites regulate autophagosome formation by delivery of an ATG8-family member, GABARAP, to the forming autophagosome membrane, the phagophore. Its proposed that GABARAP regulates phagophore expansion by activating the ULK complex. [review] PMID:28990689
GABAergic synapse is proposed as a focus of aging. (Review) PMID:28497576
Cardiolipin interaction with various Atg8 human orthologs, namely LC3B, GABARAPL2 and GABARAP, was investigated. PMID:27764541
Although neither GABARAPs nor LC3s are required for autophagosome biogenesis, loss of all Atg8s yields smaller autophagosomes and a slowed initial rate of autophagosome formation. PMID:27864321
Development of LC3/GABARAP sensors containing a LIR and a hydrophobic domain to monitor autophagy. PMID:28320742
GABRP plays an important role in placentation and this pathway may be a promising molecular target for the development of novel therapeutic strategies for preeclampsia. PMID:27221053
KBTBD6 and KBTBD7 specifically bind to GABARAP proteins.GABARAP proteins mediate localized ubiquitylation of TIAM1 by CUL3. PMID:25684205
Data show that WAC directly binds to GM130 and that this binding is required for autophagosome formation through interacting with GABARAP regulating its subcellular localization. PMID:26687599
The interaction of GABARAP with Mulan-Ube2E3 supports the role of Mulan as an important regulator of mitophagy. PMID:25224329
The FLCN-GABARAP association is modulated by the presence of either folliculin-interacting protein (FNIP)-1 or FNIP2 and further regulated by ULK1. PMID:25126726
A functional complementation of an lgg-1 null mutant with human GABARAP, its closer homolog showed that it localizes to autophagosomes and can rescue LGG-1 functions in the early embryo. PMID:25126728
PLEKHM1 regulates autophagosome-lysosome fusion through homotypic fusion and protein sorting complex and LC3/GABARAP proteins. PMID:25498145
GABARBP dramatically inhibited VEGF-induced endothelial cell proliferation, migration, and tube formation, as well as VEGFR-2 phosphorylation in vitro. PMID:24686084
knockdown of LC3B but not GABARAPs resulted in significant accumulation of p62/Sqstm1, one of the selective substrates for autophagy PMID:24582747
These results support the regulatory role of Bcl-2 in autophagy and define GABARAP as a novel interaction partner involved in this intricate connection. PMID:24240096
Taken together, our results indicate that GABARBP can regulate the pro-apoptotic activity of cisplatin via the upregulation of p53 expression. PMID:23545901
The level of GABARAPs is decreased in Lewy body disease. PMID:21684337
Evidence suggests that changes in the expression and function of GABA(A) receptors are involved in the pathogenesis of epilepsy (review) PMID:21376781
Used diffusion-ordered nuclear magnetic resonance spectroscopy to study the temperature and concentration dependence of the diffusion properties of GABARAP. PMID:20665069
DLG4/PSD95 and GABARAP were analyzed using zebrafish embryos with morpholino knockdown system as a model organism. PMID:20111057
analysis of human NSF possible binding mode to GABARAP and GATE-16 PMID:19533740
Sequence-specific 1H, 13C and 15N resonance assignments of human GABA receptor associated protein. PMID:11727985
Backbone 1H, 13C, and 15N resonance assignments for a 14 kD protein, GABA(A) receptor associated protein (GABARAP). PMID:11727986
We present the three-dimensional structure of GABARAP obtained by x-ray diffraction at 1.75 A resolution PMID:11729197
Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p PMID:11825910
Solution structure of human GABA(A) receptor-associated protein GABARAP: implications for biolgoical funcrion and its regulation PMID:11875056
Gec1/GABARAPL1 was more expressed than GABARAP in the central nervous system (CNS), while GABARAP was more expressed in endocrine glands. PMID:14625090
GABARAP mRNA expression and protein expression were significantly down-regulated in invasive ductal and invasive lobular carcinomas; GABARAP acts via a vesicle transport mechanism as a tumor suppressor in breast cancer. PMID:15695379
GABARAP and DDX 47 are involved in the apoptotic process PMID:15977068
Phosphatidylserine and phosphatidylethanolamine are targets of the mammalian Atg8 modifiers, LC3, GABARAP, and GATE-16 PMID:16303767
GABARAP does not alter receptor kinetics directly, but by facilitating surface expression of alphabetagamma receptors. PMID:16339017
Results suggest that lysosomal turnover of GABARAP-PL is activated during the differentiation of C2C12 cells to myotubes without inactivation of the mTor kinase-signaling pathway. PMID:16874098
GABARAP and DLG4 genes are involved in the etiology of nicotine dependence in European-American smokers. PMID:17164261
The association of structurally altered GABARAP protein together with GABA(A) receptor downstream of Ca2+/calmodulin-dependent protein kinase II (CAMKII) activation is essential for long-term potentiation. PMID:17581966
The X-ray structure of the soluble form of human GABA(A) receptor-associated protein complexed with a high-affinity synthetic peptide at 1.3 A resolution, is presented. PMID:18638487
Molecular modeling of a complex containing full-length calreticulin suggests a novel mode of substrate interaction, which may have functional implications for the calreticulin/calnexin family in general. PMID:19154346
The proapoptotic protein Nix/Bnip3L was found to be a potential GABARAP ligand. PMID:19363302