FOSB Recombinant Monoclonal Antibody

Code: CSB-RA594151A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, WB

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Product Details
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Target Background

Product Details

Uniprot NO.

P53539

Target Names

FOSB

Alternative Names

Protein fosB (G0/G1 switch regulatory protein 3), FOSB, G0S3

Species Reactivity

Human

Immunogen

A synthesized peptide from human FOSB protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2D12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Hela whole cell lysate(30µg), MCF-7 whole cell lysate(30µg)
All lanes: FOSB antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 36 kDa
Observed band size: 48 kDa
Exposure time：2min

Description

FOSB is a member of the Fos family of transcription factors that dimerizes with Jun proteins to form the AP-1 transcription factor complex, playing a central role in regulating gene expression in response to diverse stimuli including growth factors, cytokines, and stress signals. As an immediate-early gene product, FOSB is rapidly induced following cellular stimulation and has been implicated in critical processes ranging from cell proliferation and differentiation to neuronal plasticity and addiction-related behaviors, making it a valuable target for researchers investigating signal transduction pathways and transcriptional regulation.

This recombinant monoclonal antibody, generated in rabbit using clone 2D12, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs in controlled recombinant systems, you can expect reliable performance across experiments and between lots, eliminating the variability that can complicate long-term studies or multi-site collaborations.

The antibody has been validated for Western blot and ELISA applications, with Western blot testing demonstrating clear detection in HeLa and MCF-7 whole cell lysates at dilutions ranging from 1:500 to 1:2000. In these experiments, the observed band appears at approximately 48 kDa rather than the predicted 36 kDa molecular weight, a shift commonly attributed to post-translational modifications such as phosphorylation or glycosylation that FOSB is known to undergo during cellular activation.

Whether your research focuses on AP-1-mediated transcriptional programs, cellular stress responses, or the molecular mechanisms underlying neuroplasticity, this antibody provides a dependable tool for investigating FOSB expression and regulation in human cell models.

Customer Reviews and Q&A

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Target Background

Function(From Uniprot)

FosB interacts with Jun proteins enhancing their DNA binding activity.

Gene References into Functions

miR-144-3p plays an important role in pancreatic cancer cell proliferation, migration, and invasion by targeting FOSB PMID:28653602
FOSB immunohistochemistry is sensitive in the diagnosis of angiolymphoid hyperplasia with eosinophilia, and allows differentiation from its histological mimics PMID:29527734
FOSB overexpression results in TNBC cell death, whereas inhibition of calcium signaling eliminates FOSB induction and blocks TP4-induced TNBC cell death PMID:27248170
results shows the involvement of Spry2 in regulation of FosB and Runx2 genes, MAPK signaling and proliferation of mesenchymal stem cells. PMID:28387432
the mechanism underlying redox-regulation of AP-1 Fos/Jun transcription factors and provide structural insight for therapeutic interventions targeting AP-1 proteins. PMID:28981703
The NFATc3 first induced the expression of its interaction partner FosB before forming the heterodimeric NFATc3-FosB transcription factor complex, which bound the proximal AP-1 site in the TF gene promoter and activated TF expression. PMID:28724635
FosB expression in the prefrontal cortex and hippocampus of the cocaine addicted and depression patients PMID:27494187
FOSB is a highly sensitive and diagnostically useful marker for pseudomyogenic hemangioendothelioma. PMID:28009608
our findings show that DeltaFosB increases the expression of MMP-9 and exhibits a significantly high survival and proliferation in MCF-7 cells. PMID:26608367
Diffuse and strong FOSB expression was specific for pseudomyogenic hemangioendothelioma in the current series and FOSB immunohistochemistry is an effective tool for differentiating between PHE and its histological mimics. PMID:27515856
Our results using a number of approaches, such as promoter reporter assay, FosB knock down and Chip assay, suggest that the expression of miR-22 is regulated transcriptionally by FosB. PMID:27889568
These results suggest that SETDB1- mediated FosB expression is a common molecular phenomenon, and might be a novel pathway responsible for the increase in cell proliferation that frequently occurs during anticancer drug therapy. PMID:26949019
studies of FosB are providing new insight into the molecular basis of depression and antidepressant action. PMID:25446562
The abnormalities were screened by FISH in 44 epithelioid hemangioma (EH) from different locations with seven additional EH revealing FOSB gene rearrangements, all except one being fused to ZFP36. PMID:25043949
This study describing the expres-sion pattern of FosB/ FosB immunoreactivity inpost mortem basal ganglia sections from Parkinson disease patients. PMID:23933656
Pseudomyogenic haemangioendothelioma consistently displays a SERPINE1-FOSB fusion gene, resulting from a translocation between chromosomes 7 and 19. PMID:24374978
Data indicate a significant correlation in miR-181b, FOS and miR-21 expression glioma tissues. PMID:23810250
Report Fos-B expression in skin keratinocytes/fibroblasts and keloid fibroblasts exposed to genistein. PMID:23614275
AP-1 protein induction during monopoiesis favors C/EBP: AP-1 heterodimers over C/EBP homodimerization and stimulates FosB transcription. PMID:21543584
Induction of a DeltaFOSB mediated transcriptional pattern in the prefrontal cortex is opposite to the down-regulation observed in the nucleus accumbens in patients with major depressive disorder PMID:21616539
Findings indicate that neurobehavioral stress leads to FosB-driven increases in IL8, which is associated with increased tumor growth and metastases. PMID:20826776
transcription factor FosB/activating protein-1 (AP-1) activation is a prominent downstream signal of the extracellular nucleotide receptor P2RX7 in monocytic and osteoblastic cells PMID:20813842
RGS16 and FosB are underexpressed in pancreatic cancer with lymph node metastasis and associated with reduced survival PMID:20571966
demonstrated that a functional AP-1 site mediates MMP-2 transcription in cardiac cells through the binding of distinctive Fra1-JunB and FosB-JunB heterodimers. The synthesis of MMP-2 is considered to be independent of the AP-1 transcriptional complex PMID:12371906
AP-1 (c-Jun & FosB) binds to a site in the 5' untranslated region of the CD95L gene. Transdominant negative Jun mutants reduce CD95L promoter activity. FosB dimerized with c-Jun has an important role in TCR/CD3-mediated activation-induced cell death. PMID:12618758
In hepatoma-associated anorexia-cachexia FpsB was induced in several brain areas of thes forebrain. PMID:15926923
activation of protein kinase A elicits an immediate response through induction of genes such as ID2 and FosB, followed by sustained secretion of bone-related cytokines such as BMP-2, IGF-1, and IL-11 PMID:18490653
DeltaFosB was able to trigger partial Pref-1-mediated de-differentiation of adipocytes, which also retained their adipocytic cell phenotype. PMID:18491952
An IFN-gamma-mediated homeostatic loop limits the potential for tissue damage associated with inflammation and identifies transcriptional factor AP-1 that regulates matrix metalloproteinase expression in myeloid cells in inflammatory settings. PMID:18802113
MicroRNA-101, which is aberrantly expressed in hepatocellular carcinoma, could repress the expression of the FOS oncogene. PMID:19133651
FosB is induced in PMA treated K562 cells in a sustained manner and forms an active AP-1 protein-DNA complex. Down-regulation of FosB with specific shRNAs inhibited the induction of CD41, a specific cell surface marker of megakaryocytes. PMID:19381435
Results suggest that in addition to clinically prognostic factors, FOS-B expression has a debatable impact on patient survival. PMID:19795327