FLOT1 Recombinant Monoclonal Antibody

Code: CSB-RA008727MA1HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human, Mouse, Rat

Application: ELISA, WB, IF, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

O75955

Target Names

FLOT1

Alternative Names

FLOT 1 antibody; FLOT1 antibody; FLOT1_HUMAN antibody; Flotillin-1 antibody; Flotillin1 antibody; Integral membrane component of caveolae antibody; Reggie 2 antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human FLOT1 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

4C8B6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: HEK293 whole cell lysate(20µg), U-251MG whole cell lysate(20µg), HeLa whole cell lysate(20µg), Raji whole cell lysate(20µg), A549 whole cell lysate(20µg), HepG2 whole cell lysate(20µg)
All lanes: FLOT1 antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time：1min
Western Blot
Positive WB detected in: Rat brain tissue lysate(20µg)
All lanes: FLOT1 antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 47.4 kDa
Observed band size: 48 kDa
Exposure time: 2min
Immunofluorescence staining of NIH/3T3 cell with CSB-RA008727MA1HU at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific.
Immunofluorescence staining of Hela cell with CSB-RA008727MA1HU at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific.
Overlay Peak curve showing A431 cells stained with CSB-RA008727MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

Flotillin-1 serves as a key scaffolding protein within lipid raft microdomains, where it orchestrates membrane organization, vesicular trafficking, and signal transduction pathways. As an integral component of caveolae, FLOT1 participates in clathrin-independent endocytosis and has emerged as a significant player in studies of cell adhesion, neuronal development, and membrane dynamics. Its involvement in various disease contexts, including cancer progression and neurodegenerative disorders, makes it a compelling target for researchers investigating membrane biology and cellular signaling mechanisms.

This recombinant monoclonal antibody against FLOT1 offers the reproducibility and consistency that demanding experimental workflows require. Produced using recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The human IgG1 isotype format, combined with affinity chromatography purification, ensures high specificity for your target across experiments.

Validation data demonstrates robust performance across multiple platforms. In western blotting applications, the antibody detects a band at the expected molecular weight of approximately 47-48 kDa across diverse human cell lines including HEK293, U-251MG, HeLa, Raji, A549, and HepG2, as well as rat brain tissue. This cross-species reactivity extending to human, mouse, and rat samples provides flexibility for comparative studies. Immunofluorescence staining has been validated in both NIH/3T3 and HeLa cells, revealing characteristic membrane-associated localization patterns. Flow cytometry applications have been confirmed using A431 cells with clear separation from isotype controls.

Whether investigating lipid raft biology, membrane trafficking mechanisms, or exploring FLOT1's role in disease pathology, this antibody delivers the reliability needed for publication-quality results.

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Target Background

Function(From Uniprot)

May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.

Gene References into Functions

flotillin-1 is a tumorigenic protein that plays an important role in promoting the proliferation and tumorigenicity of LUAD. PMID:28825855
flotillin has a role in desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes PMID:27346727
Autoantibodies against flotillin-1/2 heterocomplex are present in 1-2% of patients with bona fide multiple sclerosis. PMID:28645295
Furthermore, we identify flotillin-1 (FLOT1) and histone H1 as downstream factors for cytoplasmic and nuclear pathway of S100A11, which are required for LASP1-S100A11 axis-mediated epithelial-mesenchymal transition and colorectal cancer progression. PMID:27181092
FLOT1 is associated with aggressive characteristics of HCC PMID:23840303
This study presents the endocytic pathways of internalization for muscarinic type 3 receptor and flotillin-1/2 in salivary gland epithelial cells. knockdown of flot-1 or -2 by flotillin-specific siRNA prevented internalization and reduced the endocytic efficiency of muscarinic type 3 receptor. PMID:27221048
Data show that overexpression of 4.1N protein decreased expression of flotillin-1, decreased activation of beta-catenin/Wnt pathway in non-small-cell lung cancer (NSCLC) cells. PMID:27448302
Upregulation of flotillin-1 promotes invasion and metastasis by activating TGF-beta signaling in nasopharyngeal carcinoma. PMID:26646322
Flotillin-1 protein is upregulated in human endometrial cancer and localization shifts from epithelial to stromal with increasing tumor grade PMID:26682635
Expression of flotillin-1 was associated with H-Ras in breast cancer, especially in TNBC (p < 0.001). PMID:26413934
findings revealed the expression of flotillin 1 in gastric cancer samples is significantly higher than that of noncancer/normal tissues; findings suggest that flotillin 1 may be a novel biomarker for gastric cancer PMID:25948494
miR-506 expression was down-regulated in clear cell renal cell carcinoma (ccRCC) and correlated with advanced histologic grade, clinical stage, tumor stage, positive lymph node metastasis, and distant metastasis. FLOT1, a potential target gene of miR-506, was inversely correlated with miR-506 expression in ccRCC tissues. PMID:25793370
polycystins are necessary for assembly of a novel flotillin-containing ciliary signaling complex and provide a molecular rationale for the common renal pathologies caused by OFD1 and polycystin mutations. PMID:25180832
Findings suggest that FLOT1 plays an important role in the proliferation and recurrence of transitional cell carcinoma. PMID:24890092
Downregulation of microRNA-182-5p contributes to renal cell carcinoma proliferation via activating the AKT/FOXO3a signaling pathway with FLOT1 being a target. PMID:24886554
overexpression of FLOT1 can be found in tongue squamous cell cancer patients with higher pathological stage, T classification, N classification or recurrence PMID:24695539
Flotillin-1 mRNA expression is up-regulated in non-small cell lung cancer patients. PMID:24533441
a critical role of flotillin-1 in ROS production mediated by c-Src PMID:24983503
Flotillin-1 regulates oncogenic signaling in neuroblastoma cells by regulating ALK membrane association. PMID:24830726
The FLOT1 is abundantly expressed in term villous placental CTs and endothelial cells, and in comparison, expression of these proteins in the ST is reduced. PMID:23064789
Depletion of either flotillin-1 or flotillin-2 resulted in downregulation of ErbB3 and a selective reduction of ErbB2-ErbB3 receptor complexes. PMID:24747692
study demonstrated that miR-124 might be a tumor suppressor in breast cancer via the regulation of FLOT1 PMID:24330780
We have identified flotillin 1 and 2 as new partners of the cadherin complexes PMID:24046456
These data here provide direct evidences for the molecular interaction and endocytosis of PrP(C) with Flot-1 in the presence of copper ions. PMID:23625312
Patients with higher FLOT1 expression had shorter overall survival time, whereas those with lower FLOT1 expression had longer survival time PMID:24277378
Human group V secretory phospholipase A2 is associated with lipid rafts and internalized in a flotillindependent pathway. PMID:24042857
EGFR overexpression and increase in signaling correlates with flotillin-1 expresion. PMID:24304721
Report role for flotillin 1/2 in interactions of lung epithelial cells with silica nanoparticles. PMID:22669515
we describe how flotillin-1 and -2 contribute to the stabilization of ErbB2 at the cell surface in breast cancer tissue PMID:22869152
Flotillin-1 plays an important role in Oral squamous cell carcinoma (OSCC) development. PMID:23581411
Flotillin-1 interacted with PrPc and promote the endocytosis of PrPc. PMID:23627023
results support predominant formation of flotillin-1 and -2 hetero-oligomers in resting and chemokine-stimulated human T-cells which may importantly contribute to structuring of the uropod. PMID:23012365
a novel signaling network containing FRS2, CAP and flotillin-1 PMID:22235335
receptor-tyrosine kinases may also rely on flotillin-1 upon activation, thus suggesting a general role for flotillin-1 as a novel factor in receptor-tyrosine kinase/MAP kinase signaling. PMID:22232557
Chlamydia pneumoniae intracellular growth was attenuated in the flotillin-1-silenced cells. PMID:22215737
FLOT1 plays an important role in promoting proliferation and tumorigenesis of human breast cancer and may represent a novel prognostic biomarker and therapeutic target for the disease. PMID:21447726
Flot1-enriched membrane microdomains are required for protein kinase C-regulated internalization of the DA transporter (DAT) and the glial glutamate transporter EAAT2. PMID:21399631
flotillins have a role in NPC1L1-mediated cholesterol uptake and NPC1L1-flotillins-postive cholesterol-enriched membrane microdomains are involved in the mechanism for efficient cholesterol absorption PMID:21187433
the cellular pathways used by transportan and transportan 10 (TP10) for protein transduction PMID:19348413
Data show that the BCG phagosome is relatively depleted in LAMP-2, NPC1, flotillin-1, vATPase, and syntaxin 3. PMID:19815536
In hematopoietic cells, flotillins provide intrinsic cues that govern segregation of certain microdomain-associated molecules during immune cell polarization. PMID:20027317
Flotillin-1, a marker of rafts, accumulates in lysosomes of neurons in Alzheimer's disease PMID:14708344
ATP-binding cassette, sub-family A (ABC1), member 1 forms a complex with syntaxin 13 and flotillin-1, residing at the plasma membrane and in phagosomes PMID:15469992
It is significant that there is an increased gene expression of flotillin-1 in the Parkinson substantia nigra/ventral tegmental area compared to controls. PMID:15545008
Recombinant flotillin-1 was purified and characterized in in large quantity and high purity. PMID:15939299
Myocilins with mutations such as G364V, K423E, and Y437H on the domain failed to interact with flotillin-1. PMID:16198165
Flotillin-1 small interfering RNA (siRNA) inhibited both clathrin-independent uptake of cholera toxin and endocytosis of a GPI-linked protein. We propose that flotillin-1 is one determinant of a clathrin-independent endocytic pathway in mammalian cells. PMID:16341206
flotillin-1 may recruit amyloid beta (A4) precursor protein to lipid rafts and therefore participate in the localization and processing of APP PMID:16480949
The results showed that flotillin-1 can interact with arginase1, and hence arginase activity was up-regulated by 26.8%. PMID:17113085
These lines of evidence suggested that a Gq-coupled receptor activates specifically p38 MAPK through lipid rafts and Src kinase activation, in which flotillins positively modulate the Gq signaling. PMID:17307333