EHMT2 Recombinant Monoclonal Antibody

Code: CSB-RA056037A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, WB, IHC, FC

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Target Background

Product Details

Uniprot NO.

Q96KQ7

Target Names

EHMT2

Alternative Names

Ankyrin repeat containing protein antibody; Bat 8 antibody; Bat8 antibody; C6orf30 antibody; DKFZp686H08213 antibody; EHMT 2 antibody; Ehmt2 antibody; EHMT2_HUMAN antibody; Euchromatic histone lysine methyltransferase 2 antibody; Euchromatic histone lysine N methyltransferase 2 antibody; Euchromatic histone-lysine N-methyltransferase 2 antibody; FLJ35547 antibody; G 9a antibody; G9 a antibody; G9a protein antibody; G9A antibody; G9A histone methyltransferase antibody; GAT8 antibody; H3 K9 HMTase 3 antibody; H3-K9-HMTase 3 antibody; Histone H3 K9 methyltransferase 3 antibody; Histone H3 K9 methyltransferase3 antibody; Histone H3-K9 methyltransferase 3 antibody; Histone lysine N methyltransferase antibody; Histone lysine N methyltransferase EHMT2 antibody; Histone lysine N methyltransferase; H3 lysine 9 specific 3 antibody; Histone lysine N methyltransferase; H3 lysine 9 specific3 antibody; Histone-lysine N-methyltransferase EHMT2 antibody; HLA B associated transcript 8 antibody; HLA-B-associated transcript 8 antibody; KMT 1C antibody; KMT1 C antibody; Lysine N methyltransferase 1C antibody; Lysine N-methyltransferase 1C antibody; NG 36 antibody; NG36 antibody; Protein G9a antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human EHMT2

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4A2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: HEK293 whole cell lysate(20µg),Hela whole cell lysate(20µg)
All lanes: EHMT2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 132 kDa
Observed band size: 160/170 kDa
Exposure time：1min20s
IHC image of CSB-RA056037A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing MCF-7 cells stained with CSB-RA056037A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

EHMT2, also known as G9a, is a critical histone methyltransferase responsible for mono- and dimethylation of histone H3 at lysine 9, a modification central to transcriptional silencing and heterochromatin formation. This enzyme plays essential roles in embryonic development, cell differentiation, and the maintenance of cellular identity. Dysregulation of EHMT2 has been implicated in various cancers and developmental disorders, making it a compelling target for epigenetic research and therapeutic development.

This recombinant monoclonal antibody, clone 4A2, offers the reliability that demanding epigenetic studies require. Produced using recombinant technology in rabbit host, it provides sequence-defined consistency between lots, ensuring your experimental results remain comparable across extended research timelines. The antibody was raised against a synthetic peptide derived from human EHMT2 and purified by affinity chromatography for optimal specificity.

Validation across multiple platforms demonstrates this antibody's experimental versatility. Western blot analysis in HEK293 and HeLa whole cell lysates reveals bands at 160-170 kDa, larger than the predicted 132 kDa molecular weight. This size difference likely reflects post-translational modifications, including glycosylation and automethylation events characteristic of active EHMT2. Immunohistochemistry performed on paraffin-embedded human colorectal cancer tissue shows clear nuclear staining patterns consistent with EHMT2's chromatin-associated function. Flow cytometry analysis using MCF-7 cells further confirms the antibody's utility for quantitative single-cell studies of this methyltransferase.

Whether investigating epigenetic mechanisms in cancer biology, studying chromatin dynamics during differentiation, or exploring EHMT2 as a therapeutic target, this antibody provides the validated performance needed for meaningful discoveries in histone methylation research.

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Target Background

Function(From Uniprot)

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.

Gene References into Functions

Our results demonstrate of the central role of G9a HMT in the promotion of endothelial cells proliferation, and suggest that endothelial G9a HMT may be a target in the treatment of vascular proliferative disorders and tumor neovascularization. PMID:29113759
unveil E4BP4 as a critical transcriptional modulator repressing RASSF8 expression through histone methyltransferases, G9a and SUV39H1. PMID:29467226
Results establish a direct functional link between G9a and MMP-9 in the context of pro-osteoclastogenic transcriptional programs. The model suggests that G9a mediated H3K27me1 serves as an essential mark for MMP-9 recruitment and proteolytic activity at genes encoding positive regulators of osteoclast differentiation, thus keeping them active. PMID:29807539
The study identified that decreased expression of SATB2 was correlated with tumor progression and poor prognosis in non-small cell lung cancer (NSCLC) patients. Furthermore, SATB2 suppressed lung cancer cell invasion and metastasis and regulated the expression of EMT-related proteins and histone methylation by G9a. PMID:28667416
G9a plays a role as an epigenetic mediator of hypoxic response in breast cancer PMID:28630300
Identified the tumor suppressor RARRES3 as a critical target of G9a. Epigenetic silencing of RARRES3 contributed to the tumor-promoting function of G9a PMID:28532996
regulatory pathway based on casein kinase 2-G9a-RPA permits homologous recombination in cancer cells PMID:28698370
these findings reveal a role for REST-associated G9a and histone H3K9 methylation in the repression of USP37 expression in medulloblastoma. Reactivation of USP37 by G9a inhibition has the potential for therapeutic applications in REST-expressing medulloblastomas PMID:28483947
Regulated methylation and phosphorylation serve as a switch controlling G9a and GLP coactivator function, suggesting that this mechanism may be a general paradigm for directing specific transcription factor and coregulator actions on different genes. PMID:28615290
interleukin-8 is a downstream effector of G9a to increase gemcitabine resistance in pancreatic cancer. Overexpression of G9a correlated with poor survival and early recurrence in pancreatic cancer patients. PMID:27531902
Taken together, our findings provide evidence that G9a protects head and neck squamous cell carcinomas (HNSCC)cells against chemotherapy by increasing the synthesis of GSH, and imply G9a as a promising target for overcoming cisplatin resistance in HNSCC PMID:28265008
In this study, EHMT2 (a histone methyltransferase) was determined to be significantly overexpressed in breast cancer tissues and in Oncomine data. In addition, knockdown of EHMT2 reduced cell migration and invasion. PMID:29337058
Knockdown of G9a increased the sensitivity of cells to radiation treatment and sensitized cells to DNA damage agents through PP2A-RPA axis. PMID:28351524
EHMT2 can directly repress Beclin-1 and the inhibition of EHMT2 may be a useful therapeutic approach for cancer prevention by activating autophagy PMID:27174920
Loss of miR-1 and gene copy number gain of G9a contributed to its frequent upregulation in liver cancer, which epigenetically silenced the expression of tumor suppressor RARRES3, and ultimately contributed to hepatocellular carcinoma (HCC) cell proliferation and migration. PMID:28532996
G9a promotes breast cancer by regulating iron metabolism through the repression of ferroxidase hephaestin. PMID:28819251
study uncovers a novel mechanism of G9A promoting tumor cell growth and invasion by silencing CASP1, and implies that G9A may serve as a therapeutic target in treating Non-small-cell lung cancer. PMID:28383547
G9a inhibition impairs anchorage-dependent and -independent cell growth in hepatocellular carcinoma cells PMID:28423509
A histone H3K9-like mimic within LIG1 is methylated by G9a and GLP and avidly binds UHRF1. Interaction with methylated LIG1 promotes the recruitment of UHRF1 to DNA replication sites and is required for DNA methylation maintenance. PMID:28803780
G9a is an important regulator in placental diseases caused by defective vascular maturation. PMID:28455378
Low G9a expression is associated with lung and colonic cancer. PMID:27452519
G9A overexpression is associated with peritoneal fibrosis. PMID:28278257
G9a inhibition potentiates the anti-tumour activity of DNA double-strand break inducing agents by impairing DNA repair independent of p53 status. PMID:27431310
Inhibition of EZH2 (by GSK-343 or EPZ-6438) or inhibition of EHMT2 (by UNC-0638) in the Th17 primary cell model of HIV latency or resting memory T cells isolated from HIV-1-infected patients receiving highly active antiretroviral therapy, was sufficient to induce the reactivation of latent proviruses. PMID:28246360
we find an estrogen receptor-independent synthetic lethal interaction between a GATA3 frameshift mutant with an extended C-terminus and the histone methyltransferases G9A and GLP, indicating perturbed epigenetic regulation PMID:27588951
High expression of G9a and H3K9me2 in glioma tissue samples was associated with the WHO grade, which indicated that G9a and H3K9me2 may promote generation and development of glioma. BIX01294 may be a potential novel therapeutic agent in the treatment of glioma. PMID:27748874
Depletion of G9a inhibits cell growth and induces cells apoptosis in gastric cancer. PMID:27081761
G9a and GLP are required for stable maintenance of imprinted DNA methylation in embryonic stem cells. PMID:27052169
The G9a exon 10(+) isoform is necessary for neuron differentiation and regulates the alternative splicing pattern of its own pre-mRNA, enhancing exon 10 inclusion. PMID:26997278
G9a promotes H3K27 methylation of the E-cadherin promoter by upregulating PCL3 to increase PRC2 promoter recruitment and by downregulating the H3K27 demethylase KDM7A to silence E-cadherin gene PMID:26688070
High G9a contributes to endometrial cancer progression. PMID:25613390
LASP-1 associated with UHRF1, G9a, Snail1 and di- and tri-methylated histoneH3 in a CXCL12-dependent manner based on immunoprecipitation and proximity ligation assays PMID:25982273
Along with the PHF20/MOF complex, G9a links the crosstalk between ERalpha methylation and histone acetylation that governs the epigenetic regulation of hormonal gene expression. PMID:26960573
high expression in hepatocellular carcinoma associated with worse outcome PMID:26765460
The present study aimed at examining the potential role of autophagy in the anti-proliferation effect of G9a inhibition on transitional cell carcinoma T24 and UMUC-3 cell lines in vitro. PMID:26397365
Homocysteine Induces Collagen I Expression by Downregulating Histone Methyltransferase G9a PMID:26192994
G9a has a dominant function in transcriptional repression of potassium channels and in acute-to-chronic pain transition. PMID:26551542
KMT1c (G9a) is markedly up regulated after differentiation of monocytes into immature dendritic cells. PMID:25843229
This study demonstrate that the increases in a restrictive epigenome seen in schizophrenia are sex dependent. Specifically,G9A were significantly increased in lymphocytes from men with schizophrenia. PMID:25935252
Data indicate zinc finger proteins ZNF644 and WIZ as two core subunits in the histone-lysine N-methyltransferase G9a/GLP complex, and interact with the transcription activation domain of G9a and GLP. PMID:25789554
data provide genetic and pharmacologic evidence that EHMT1 and EHMT2 are epigenetic regulators involved in gamma-globin repression and represent a novel therapeutic target for SCD. PMID:26320100
G9a protein is essential for the induction of epithelial-to-mesenchymal transition and cancer stem cell-like properties in HNSCC PMID:25749385
Down-regulation of G9a triggers DNA damage response and inhibits colorectal cancer cells proliferation. PMID:25595900
The expression level of EHMT1 and EHMT2 inversely correlates with the type I interferon responsiveness in chronic myeloid leukemia cell lines. PMID:25079219
G9a-dependent H3K9me2 repression on CD133 and Sox2 was one of the main switches of the self-renewal in glioma cancer stem cells. PMID:24833465
The current knowledge on the mechanisms of action and function of EHMT2, with particular emphasis on their interplay in the regulation of chromatin states and biological processes. PMID:25365549
MCM7 and G9a may serve as effective prognostic factors and could also be used as biomarkers for predicting various clinical outcomes of oesophageal squamous cell carcinoma PMID:24805087
we defined a role for activated STAT3 and G9a histone methyltransferase in epigenetic silencing of miR-200c, which promotes the formation of breast CSCs defined by elevated cell surface levels of the leptin receptor PMID:25840984
G9a contributes to multiple steps of ovarian cancer metastasis. PMID:25115793
H3K9 methyltransferase G9a plays role in the apoptosis and autophagy in oral squamous cell carcinoma. PMID:25634693

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Subcellular Location

Nucleus. Chromosome. Note=Associates with euchromatic regions. Does not associate with heterochromatin.

Protein Families

Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, Suvar3-9 subfamily

Tissue Specificity

Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.

Database Links

HGNC: 14129

UNIGENE: Hs.709218

KEGG: hsa:10919

STRING: 9606.ENSP00000364687

OMIM: 604599

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