DLAT Recombinant Monoclonal Antibody

Code: CSB-RA283972A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human, Mouse

Application: ELISA, WB, IHC, FC

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Target Background

Product Details

Uniprot NO.

P10515

Target Names

DLAT

Alternative Names

70 kDa mitochondrial autoantigen of primary biliary cirrhosis antibody; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex antibody; Dihydrolipoamide S Acetyltransferase antibody; Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex antibody; dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial antibody; DLAT antibody; DLTA antibody; E2 antibody; E2 component of pyruvate dehydrogenase complex antibody; M2 antigen complex 70 kDa subunit antibody; mitochondrial antibody; ODP2_HUMAN antibody; PBC antibody; PDC E2 antibody; PDC-E2 antibody; PDCE2 antibody; Pyruvate dehydrogenase complex component E2 antibody; Pyruvate dehydrogenase complex E2 subunit antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human DLAT protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5C6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: HepG2 whole cell lysate(30µg), K562 whole cell lysate(30µg), COLO205 whole cell lysate(30µg), MCF7 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), A549 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), U251 whole cell lysate(30µg), Mouse brain tissue lysate(30µg)
All lanes:DLAT antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time：2min
IHC image of CSB-RA283972A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA283972A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing MCF-7 cells stained with CSB-RA283972A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Description

Dihydrolipoamide acetyltransferase, encoded by the DLAT gene, serves as the E2 component of the pyruvate dehydrogenase complex, a critical mitochondrial enzyme system that links glycolysis to the citric acid cycle. This protein has garnered significant research attention due to its role as the 70 kDa mitochondrial autoantigen in primary biliary cirrhosis, making it a valuable target for both metabolic studies and autoimmune disease research.

This recombinant rabbit monoclonal antibody, clone 5C6, offers researchers the reproducibility and consistency that comes with sequence-defined production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures that every lot performs identically, eliminating the variability that can complicate long-term studies or multi-site collaborations.

Extensive validation demonstrates this antibody's versatility across multiple experimental platforms. Western blot analysis confirms specific detection at the expected 69 kDa molecular weight across a diverse panel of human cell lines including HepG2, K562, COLO205, MCF7, Jurkat, A549, PC-3, and U251, as well as mouse brain tissue, supporting the stated cross-species reactivity with human and mouse samples. Immunohistochemistry validation in paraffin-embedded human kidney and liver cancer tissues shows reliable staining with citrate buffer antigen retrieval, while flow cytometry analysis in MCF-7 cells demonstrates clear positive signal separation from isotype controls.

The antibody arrives in a stabilized liquid format containing glycerol for convenient aliquoting and long-term storage. Whether investigating mitochondrial metabolism, pyruvate dehydrogenase complex function, or autoimmune mechanisms in primary biliary cirrhosis, this antibody provides a dependable tool for signal transduction research across multiple validated applications.

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Target Background

Function(From Uniprot)

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

Gene References into Functions

DLAT interacts with C1QBP in mitochondria. PMID:26753982
ongoing activation of PDCE2-specific B-cells in primary biliary cirrhosis PMID:25043065
Results show that DLAT and ACAT2 as upstream acetyltransferases of K76 and K294 in 6PGD protein. PMID:25042803
These findings were used to identify potentially antigenic sequences within PDC-E2 (an important hepatic autoantigen) that contain a DR0801 motif. PMID:23543758
Solution structure and characterisation of the human pyruvate dehydrogenase complex core assembly PMID:20361979
Data suggest that the catalytic site of pyruvate dehydrogenase complex E2 rather than the previously reported lipoyl binding peptide may contain immunodominant epitopes recognized by antimitochondrial antibodies in primary biliary cirrhosis. PMID:20180236
a novel HLA-A*0201-restricted epitope PDC-E2 165 to 174 was defined in patients with primary biliary cirrhosis. PMID:12395322
study of facilitated interaction between the pyruvate dehydrogenase kinase isoform 2 and the dihydrolipoyl acetyltransferase PMID:12816949
model of the pyruvate dehydrogenase complex formed by E2 and E2 plus the E3-binding protein and binding of the E1 and E3 components PMID:14638692
Having found that there is an excellent and almost unique match between the PDC-E2 autoepitope and a sequence in mycobacterial hsp65s, we tested the corresponding peptides for cross-reactivity using sera from 90 Spanish and British PBC patients. PMID:15120760
This study report two unrelated patients with pyruvate dehydrogenase deficiency caused by defects in the E2 subunit. PMID:16049940
epitope specificity of these PDC-E2 autoantibodies was distinctive suggesting that the mechanisms leading to loss of tolerance in the transplantation patients are distinct from primary biliary cirrhosis PMID:17068145
Species specificity in the interaction between hE1beta and hE2 in pyruvate dehydrogenase complex. PMID:18206651
tissue specificity of the autoimmune injury in primary biliary cirrhosis is a consequence of the unique characteristics of HIBECs during apoptosis and can be explained by exposure to immune system of intact immunoreactive PDC-E2 within apoptotic blebs. PMID:19185000