DCLK1 Recombinant Monoclonal Antibody

Code: CSB-RA033218A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, IHC, IF, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

O15075

Target Names

DCLK1

Alternative Names

Serine/threonine-protein kinase DCLK1 (EC 2.7.11.1) (Doublecortin domain-containing protein 3A) (Doublecortin-like and CAM kinase-like 1) (Doublecortin-like kinase 1), DCLK1, DCAMKL1 DCDC3A KIAA0369

Species Reactivity

Human

Immunogen

A synthesized peptide from human DCLK1 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8B3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA033218A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of MCF-7 cell with CSB-RA033218A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA033218A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

Doublecortin-like kinase 1 (DCLK1) is a serine/threonine kinase that has emerged as a critical marker in stem cell biology and cancer research. Originally characterized for its role in neuronal migration and brain development, DCLK1 has gained significant attention as a marker of tumor-initiating cells in gastrointestinal cancers, making it a compelling target for researchers investigating cancer stem cell populations, epithelial homeostasis, and therapeutic resistance mechanisms.

This recombinant rabbit monoclonal antibody, clone 8B3, offers the consistency and reproducibility that demanding research applications require. Generated against a synthetic peptide derived from human DCLK1, the antibody benefits from recombinant production technology, ensuring sequence-defined specificity and eliminating the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. Affinity chromatography purification further enhances signal clarity by removing non-specific immunoglobulins.

Validation across multiple detection platforms demonstrates this antibody's experimental versatility. Immunohistochemistry studies in paraffin-embedded human small intestine tissue confirm reliable detection in formalin-fixed samples, which is particularly relevant given DCLK1's established role in intestinal stem cell biology. Immunofluorescence analysis in MCF-7 breast cancer cells reveals clear cytoplasmic localization patterns, while flow cytometry validation using SH-SY5Y neuroblastoma cells demonstrates robust signal separation from isotype controls, enabling quantitative assessment of DCLK1-positive populations.

The unconjugated format provides flexibility for researchers to pair this antibody with their preferred detection systems across immunohistochemistry, immunofluorescence, flow cytometry, and ELISA workflows. Whether investigating DCLK1's function in neural development, characterizing tumor stem cell populations, or exploring its kinase activity in cellular signaling, this antibody delivers the performance consistency essential for meaningful experimental outcomes.

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Target Background

Function(From Uniprot)

Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.

Gene References into Functions

DCLK1 plays an important role in cancer progression and is involved in the regulation of miR-200c expression. PMID:29656186
The expression of DCLK1 was found in low-grade adenomas and increased with worsening severity of dysplasia. DCLK1 expression was highly observed in advanced adenomas, which had a clinically higher malignant potential. PMID:29475922
High DCLK1 expression is associated with malignant pleural mesothelioma. PMID:28560410
On the basis of these data, FOXD3 is a potent repressor of DCLK1-S expression in normal cells; loss of FOXD3 in hCCCs/hCRCs allows upregulation of DCLK1-S, imparting a potent invasive potential to the cells PMID:28851816
Expression levels of phosphorylated cdc25A (p-cdc25A) and phosphorylated Chk1 (p-Chk1), belonging to the ATR pathway, were decreased by treatment with Dclk1 inhibitor LRRK2-IN-1 (LRRK), indicating Dclk1 involvement in the ATR pathway PMID:29048622
In rectal cancer patients treated with preoperative chemoradiotherapy (CRT), DCLK1 expression was significantly correlated with lymph node metastasis and poor cancer-specific survival. However, in patients treated without preoperative therapy, no such correlation was found. DCLK1 expression can be associated with lymph node metastasis and poor cancer-specific survival in rectal cancer patients who receive CRT. PMID:29924454
Pancreatic ductal adenocarcinoma patients with DCLK1-positive tumors had significantly shorter survival times than those with DCLK1-negative tumors. PMID:28883702
DCLK1 plays crucial role in the regulation of epithelial-mesenchymal transition.DCLK1 is highly expressed in pancreatic neuroendocrine tumor. PMID:28179411
DCLK1 is a prognostic biomarker for shortened survival. Secondly, through inhibition of DCLK1, it may serve as a therapeutic target as well PMID:28351564
DCLK1 has been identified as a potential target for miR-448 to regulate lung squamous cell carcinoma cells growth. PMID:28320089
Generated a (doublecortin like kinase 1) DCLK1-S-specific polyclonal antibody (S-isoform specific), and studied its use in screening of colon cancer after colonoscopy. PMID:28414327
DCLK1 expression was observed in tumor cells in patients with pathological stage I non-small cell lung cancer (NSCLC)and was correlated with adverse prognosis, especially in patients with adenocarcinoma. DCLK1 may be a potential new therapeutic target PMID:28382517
Biochemical and structural characterization of DCLK1 allowed for the mapping of cancer-causing mutations within the kinase domain. PMID:27545623
Hence, we outline and discuss emerging evidence that strongly supports tuft cells as key regulatory cells in the complex network of the intestinal microenvironment. [review] PMID:28684459
Our results indicate that Dclk1 is essential in advancing intestinal tumorigenesis. Knocking down Dclk1 decreases tumor stemness and progression and is thus predicted to regulate pro-survival signaling and tumor cell pluripotency. PMID:28148261
The univariate and multivariate analyses suggested DCAMKL1 protein overexpression was an unfavorable prognostic factor in bladder cancer patients. In conclusion, DCAMKL1 is an independent poor prognostic factor for bladder cancer patients. PMID:28621231
DCLK1 induction and its overexpression following hepatic injury are likely to contribute to tumorigenesis including maintenance and dissemination of tumor cells in circulation. PMID:27694285
first evidence for the suppressive activity of miR-613 in hepatocellular carcinoma, which is causally linked to targeting of DCLK1 PMID:27049311
MSX1 and DCLK1 might be used in colorectal cancer detection or as target of cancer therapies. PMID:27966796
Increased expression of stromal DCLK1 was detected in endometriotic tissue compared to endometriosis patient endometrium. Stromal expression of DCLK1 was increased in endometrium of endometriosis patients compared to controls. PMID:27881125
DCLK1 up-regulation may play a contributory role in colorectal cancer metastasis and poor prognosis via activation of EMT. DCLK1 may serve as an independent predictor for CRC prognosis. PMID:27520310
Results showed the mechanism in which miR-137 regulates the expression of DCLK1, and demonstrated the opposite expression patterns of miR-137/DCLK1 in human non-small cell lung carcinoma and colon cancer stem cells. PMID:26747706
DCLK1 promoter methylationis associated with lung cancer. PMID:26311076
DCLK1 expression could aid in the prognostication and management of breast cancer with neuroendocrine differentiation. PMID:26621833
DCLK1 controls these complex cellular signaling pathways to regulate hepatocellular carcinoma growth, and may be used as a prognostic biomarker. PMID:26468984
results provide new insights into the molecular mechanism of the hepatitis B/C-virus induced liver inflammation and tumorigenesis via DCLK1-controlled networks PMID:25948779
Data suggest that doublecortin and CaM kinase-like 1 protein short-transcripts (DCLK1-S) may represent an important target for preventing/inhibiting colon-cancers, and for eliminating colon-cancer-stem-cells (CSCs). PMID:26447334
DCLK1 is essential for the invasive and metastatic properties of pancreatic cancer stem cells. PMID:26764906
Data indicate that doublecortin-like kinase 1 (DCLK1) is overexpressed and has a unique methylation signature in renal clear cell carcinoma (RCC). PMID:25605241
Doublecortin-like kinase 1 is elevated serologically in pancreatic ductal adenocarcinoma and widely expressed on circulating tumor cells. PMID:25723399
Increased expression of DCLK1 was observed in the epithelium, stroma and plasma of patients with Barrett's esophagus and esophageal adenocarcinoma. PMID:25283374
Potential exist for DCLK1 as a colorectal cancer biomarker for early detection, but may also have clinical implications regarding the previously proposed therapy toward DCLK1-positive cancer cells. PMID:24384857
XMD8-92 treatment results in inhibition of DCLK1 and downstream oncogenic pathways. PMID:24880079
RNA interference-mediated silencing of DCLK1 triggers apoptotic cell death of colon cancer cells in vitro and in vivo. PMID:24626093
DCLK1 mRNA levels were considerably reduced during FLV treatment. PMID:24260365
DCLK1 regulates pluripotency and angiogenic factors via microRNA-dependent mechanisms in pancreatic cancer. PMID:24040120
Human pancreatic ductal adenocarcinoma cells contain morphologically and functionally distinct subpopulations, expressing DCLK1, that have cancer stem cell-like properties. PMID:24096005
DCAMKL1 represses osteoblast activation by antagonizing Runx2, the master transcription factor in osteoblasts. PMID:23918955
Dclk1 distinguishes between tumor and normal stem cells in the intestine. PMID:23202126
DCAMKL-1 marks a subset of colorectal stem cells, as well as a subset of entero-endocrine cells. PMID:22749579
the progressive increase of DCAMKL-1 expression in Barrett's esophagus from dysplasia to esophageal adenocarcinoma PMID:21916995
Several DCLK1 variants were associated with disease phenotypes for rs7989807 in intron 3 (combination of SCZ, ADHD and BP), intron 19 (SCZ+BP) and the 3'UTR (SCZ+BP). PMID:22539971
High DCAMKL1 is associated with HCV-induced hepatocarcinogenesis. PMID:21937640
Eight suggestive significant loci were detected with a series of genes expressed within the inner ear that underlie the auditory function, such as: DCLK1, PTPRD, GRM8, CMIP. PMID:21493956
Data show that siRNA-mediated knockdown of DCAMKL-1 in pancreatic cancer cells induced microRNA miR-200a, an EMT inhibitor, along with downregulation of EMT-associated transcription factors. PMID:21285251
Together, our results suggest that silencing the DCLK splice variants DCL and DCLK-long induces apoptosis in NB cells. PMID:20228126
genomic organization underlying the splice variants of DCLK and the expression profile of two splice variants affecting the kinase domain of DCLK and CPG16 (candidate plasticity gene 16). PMID:11884394
high-quality wild-type crystals of doublecortin-like kinase were obtained and a complete native data set was collected to 1.5 A resolution PMID:12595708
an examination of the binding sites on this protein PMID:12692530

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Protein Families

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily

Tissue Specificity

In fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The ty

Database Links

HGNC: 2700

UNIGENE: Hs.507755

KEGG: hsa:9201

STRING: 9606.ENSP00000255448

OMIM: 604742

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