CTCF Recombinant Monoclonal Antibody

Code: CSB-RA011511A0HU

Rate （N/A）
Review （0）

Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, IF, FC

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

P49711

Target Names

CTCF

Alternative Names

Transcriptional repressor CTCF (11-zinc finger protein) (CCCTC-binding factor) (CTCFL paralog), CTCF

Species Reactivity

Human

Immunogen

A synthesized peptide from human CTCF protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2D9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Immunofluorescence staining of Hela cell with CSB-RA011511A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing jurkat cells stained with CSB-RA011511A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

CTCF serves as a master architectural protein in chromatin organization, functioning as an essential regulator of three-dimensional genome structure and gene expression. This 11-zinc finger transcription factor establishes chromatin boundaries, mediates long-range DNA interactions, and plays critical roles in genomic imprinting and X-chromosome inactivation. Given its involvement in maintaining proper gene regulation, CTCF has become a key target for researchers investigating chromatin biology, epigenetic mechanisms, and cancer-associated genomic instability.

This recombinant monoclonal antibody, generated against a synthetic peptide from human CTCF, offers the reproducibility and consistency that demanding chromatin studies require. The recombinant production method ensures sequence-defined specificity and eliminates the lot-to-lot variability that can compromise longitudinal experiments or multi-site collaborations. Clone 2D9 provides researchers with a reliable tool for detecting this nuclear protein across multiple experimental platforms.

Validation studies demonstrate strong performance in immunofluorescence and flow cytometry applications. Immunofluorescence analysis of HeLa cells reveals clear nuclear localization consistent with CTCF's role as a chromatin-associated factor, with optimal staining achieved at dilutions between 1:50 and 1:200. Flow cytometry validation using Jurkat cells confirms specific detection with distinct positive signal separation from isotype controls, supporting quantitative analysis of CTCF expression in suspension cell populations.

The antibody is supplied in a stabilized liquid format with glycerol for convenient long-term storage at -20°C or -80°C. Whether investigating chromatin loop formation, exploring boundary element function, or examining CTCF dysregulation in disease contexts, this antibody provides the specificity and reproducibility needed for meaningful experimental outcomes in epigenetics and gene regulation research.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays an important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19. Plays a role in the recruitment of CENPE to the pericentromeric/centromeric regions of the chromosome during mitosis.

Gene References into Functions

The authors propose that cellular CCCTC-binding factor binding at the herpes simplex virus 1 CCCTC-binding factor binding sites (CTRL2) acts as a chromatin insulator to keep viral chromatin in a form that is poised for reactivation, a state which we call poised latency. PMID:29437926
neither the deletion of the CTCF locus nor the ectopic insertion of Firre cDNA or its ectopic expression are sufficient to alter topologically associated domains in a sex-specific or allele-specific manner PMID:29654311
CTCF maintains regulatory homeostasis of cancer pathways. PMID:30086769
these results suggest that CTCF participate in DNA damage response via poly(ADP-ribosylation). PMID:28262757
Studies suggest that the connection between DNA-binding protein CTCF (CTCF), cohesin, chromatin structure, and behavior is important in understanding of the development of behavior in general, and neurodevelopmental disorders in particular [Review]. PMID:29110030
ID1, CTCF and ELK1 may be associated with prostate cancer, and may be potential therapeutic targets for the treatment of this disease. PMID:29956775
CTCF promotes HSV-1 lytic transcription by facilitating the elongation of RNA Pol II and preventing silenced chromatin on the viral genome. PMID:28045091
These results, together with a prior exomesequencing based study, suggest that CTCF mutations may be involved in the development of ovarian endometriosis. PMID:29845264
findings establish for the first time that CTCF is an important regulator of the homologous recombination repair pathway. PMID:28560323
Findings indicate that CCCTC-binding factor (CTCF)-driven doublesex and mab-3 related transcription factor 2 protein (TERRA) transcription acts in cis to facilitate telomere repeat replication and chromosome stability. PMID:29235471
poly(ADP-ribosyl)ated CTCF changes its DNA binding and localisation in a breast cell line which is associated with nucleosome repositioning. PMID:29981477
Here, we show that PARP1 and host insulator protein CTCF colocalize at specific sites throughout the EBV genome and provide evidence to suggest that PARP1 acts to stabilize CTCF binding and maintain the open chromatin landscape at the active Cp promoter during type III latency. Further, PARP1 activity is important in maintaining latency type-specific viral gene expression. PMID:29976663
HOTTIP cooperates with CTCF to coordinate HOXA gene expression. PMID:29698677
CD4(+) T cells showed the greatest increase (threefold) in ORMDL3 expression in individuals carrying the asthma-risk alleles, where ORMDL3 negatively regulated interleukin-2 production. The asthma-risk variants rs4065275 and rs12936231 switched CTCF-binding sites in the 17q21 locus. PMID:27848966
Our data reveal that vigilin is essential for maintenance of imprinting of IGF2 gene via functional interaction between KH1-7 domains of vigilin and zinc-finger domains of CTCF. PMID:29157910
This study confirms that haploinsufficiency of CTCF causes distinct clinical features, and that a microdeletion encompassing CTCF could cause a recognisable CTCF deletion syndrome. Perturbed DNA methylation at CTCF binding sites, not at imprinted loci, may underlie the pathomechanism of the syndrome. PMID:28848059
structural studies show that the sequence-specific interactions between zinc fingers and CTCF-binding sites determine the directionality and conservation of CTCF recognition. PMID:29076501
CTCF may be a key factor that contributes to gene co-mutations in cancer. PMID:27762310
results support a model in which YY1 acts as an architectural protein to connect developmentally regulated looping interactions; the location of YY1-mediated interactions may be demarcated in development by a preexisting topological framework created by constitutive CTCF-mediated interactions. PMID:28536180
The MeCP2, a protein whose mutated forms are involved in Rett syndrome; and CTCF, a constitutive transcriptional insulator. PMID:28796949
The results show that cohesin has an essential genome-wide function in mediating long-range chromatin interactions and support the hypothesis that cohesin creates these by loop extrusion, until it is delayed by CTCF in a manner dependent on PDS5 proteins, or until it is released from DNA by WAPL. PMID:29217591
CTCF-FOXM1 axis regulates tumour growth and metastasis in hepatocellular carcinoma cells. PMID:28862757
we show CTCF binding site mutations to be functional by demonstrating allele-specific reduction of CTCF binding to mutant alleles. While topologically associating domains with mutated CTCF anchors in melanoma contain differentially expressed cancer-associated genes, CTCF motif mutations appear generally under neutral selection PMID:27974201
CTCF-mediated long-range interactions are integral for a multitude of topological features of interphase chromatin, such as the formation of topologically associated domains, domain insulation, enhancer blocking and even enhancer function. PMID:26802288
Authors have identified two novel pro-tumorigenic roles (promoting cell survival and altering cell polarity) for genetic alterations of CTCF in endometrial cancer. PMID:28319062
Describe several protein-DNA complex structures of a human CTCF tandem zinc-finger array, explaining the adaptability of CTCF to sequence variations and the positiondependent effect of differential DNA methylation at two cytosine residues, and revealing a potential function of C-terminal ZF8 and ZF9 spanning across the DNA phosphate backbone. PMID:28529057
CCCTC-binding factor (CTCF) targets the binding sites within MYCN promoter to facilitate its expression in neuroblastoma (NB) cells. PMID:26549029
we review recent high-resolution chromosome conformation capture and functional studies that have informed models of the spatial and regulatory compartmentalization of mammalian genomes, and discuss mechanistic models for how CTCF and cohesin control the functional architecture of mammalian chromosomes. PMID:27089971
GAD1 is reactivated by DNA methylation, which provided a model for DNA methylation and the active orchestration of oncogenic gene expression by CTCF in cancer cells. PMID:26549033
epigenetic factor CTCF-mediated chromatin remodeling regulates interactions between eye-specific PAX6 and those genes that are induced/associated with cell differentiation to modulate corneal epithelial cell-specific differentiation PMID:27583466
Rta-mediated decreased binding of CTCF in the viral genome is concurrent with virus reactivation. Via interfering with CTCF binding, in the host genome Rta can function as a transcriptional repressor for gene silencing. PMID:28490592
although we were unable to detect HD-associated DNA methylation alterations at queried sites, we found that DNA methylation may be correlated to the age of disease onset in cortex tissues. Moreover, our data suggest that DNA methylation may, in part, contribute to tissue-specific HTT transcription through differential CTCF occupancy. PMID:26953320
vitamin D-sensitive CTCF sites provide further mechanistic details to the epigenome-wide understanding of 1,25(OH)2D3-mediated gene regulation PMID:27569350
These findings indicate that erythroid specific activator GATA-1 acts at CTCF sites around the beta-globin locus to establish tissue-specific chromatin organization. PMID:28161276
TOP2B is positioned to solve topological problems at diverse cis-regulatory elements and its occupancy is a highly ordered and prevalent feature of CTCF/cohesin binding sites that flank TADs. PMID:27582050
CTCF binding to eRNAs and promoters is facilitated by estrogen when chromatin establishes contacts with nuclear lamina. PMID:27638884
we investigated the cell-type specificities of CTCF sites related to these functions across five cell types. Our study provides new insights into the multivalent functions of CTCF in the human genome PMID:27067545
The results indicate that the initial chromatin conformation affects subsequent RA-induced HOXA gene activation. Our study uncovers that a removable insulator spatiotemporally switches higher-order chromatin and multiple gene activities via cooperation of CTCF and key transcription factors. PMID:27798106
we find no evidence for selection driving these distinctive patterns of mutation. The mutational load at CTCF-binding sites is substantially determined by replication timing and the mutational signature of the tumor in question, suggesting that selectively neutral processes underlie the unusual mutation patterns. PMID:27490693
data demonstrate a reciprocal relationship between Hmgb2 and Ctcf in controlling aspects of chromatin structure and gene expression. PMID:27226577
Study described the formation of mutually exclusive complexes of ENY2 with insulator proteins and Sgfl1-a component of the SAGA complex, direct binding partner for ENY2 PMID:27417714
This is supported by the depletion of CTCF in glioblastoma cells affecting the expression levels of NOTCH2 as a target of miR-181c. CONCLUSION: Together, our results point to the epigenetic role of CTCF in the regulation of microRNAs implicated in tumorigenesis PMID:26983574
Our data show that aberrant epigenetic inactivation of DUSP2 occurs in carcinogenesis and that CTCF is involved in the epigenetic regulation of DUSP2 expression. PMID:26833217
Mutational analysis highlighted a potential role for CTCF, a crucial regulator of long-range chromatin interactions, in head and neck cancer progression. PMID:26747525
CTCF and cohesins shape the genome during evolution. (Review) PMID:26439501
The action of SNF2H at CTCF sites is functionally important as depletion of CTCF or SNF2H affects transcription of a common group of genes. PMID:27019336
CSB and CTCF can regulate each other's chromatin association, thereby modulating chromatin structure and coordinating gene expression in response to oxidative stress. PMID:26578602
CTCF binds to the provirus at a sharp border in epigenetic modifications in the pX region of the HTLV-1 provirus in T cells naturally infected with HTLV-1. This may cause widespread abnormalities in host cell chromatin structure and gene expression. PMID:26929370
CTCF/cohesin coordinates HOXA cluster higher-order chromatin structure and expression during development PMID:26376810
CTCF has a role in regulating SLC45A3-ELK4 Chimeric RNA PMID:26938874

Hide All

Subcellular Location

Nucleus, nucleoplasm. Chromosome. Chromosome, centromere. Note=May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase.

Protein Families

CTCF zinc-finger protein family

Tissue Specificity

Ubiquitous. Absent in primary spermatocytes.

Database Links

HGNC: 13723

UNIGENE: Hs.368367

KEGG: hsa:10664

STRING: 9606.ENSP00000264010

OMIM: 604167

Promotions