COL1A2 Recombinant Monoclonal Antibody

Code: CSB-RA005728MA1HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, IHC

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Product Details
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Target Background

Product Details

Uniprot NO.

P08123

Target Names

COL1A2

Alternative Names

COL1A2 Recombinant Monoclonal Antibody

Species Reactivity

Human

Immunogen

Recombinant Human COL1A2 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

mFc2a

Clone No.

3C3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA005728MA1HU diluted at 1:50 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Description

COL1A2 encodes the alpha-2 chain of type I collagen, the most abundant structural protein in the human body and a critical component of skin, tendon, bone, and connective tissue extracellular matrix. This protein plays essential roles in tissue architecture, wound healing, and fibrotic processes, making it a key target for researchers investigating connective tissue disorders, cancer progression, and stem cell differentiation. Mutations in COL1A2 are directly linked to osteogenesis imperfecta type IV, underscoring its clinical significance in skeletal biology research.

This recombinant monoclonal antibody, clone 3C3, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures comparable results across experiments. The antibody was raised against recombinant human COL1A2 protein and has been validated for human sample reactivity.

Validation studies demonstrate reliable performance in immunohistochemistry applications, with testing conducted on paraffin-embedded human prostate cancer tissue. Using a Leica Bond system with citrate buffer antigen retrieval and a working dilution of 1:50, the antibody produced clear specific staining when detected with an HRP-conjugated secondary antibody and DAB visualization. This validation in tumor tissue is particularly relevant for researchers examining collagen remodeling in the cancer microenvironment. The recommended dilution range of 1:50 to 1:200 for immunohistochemistry provides flexibility for optimization across different tissue types and fixation conditions.

Supplied in a glycerol-based PBS buffer for stability, this antibody serves researchers exploring signal transduction pathways, stem cell biology, and the broader landscape of extracellular matrix dynamics in health and disease.

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Target Background

Function(From Uniprot)

Type I collagen is a member of group I collagen (fibrillar forming collagen).

Gene References into Functions

Osteogenesis imperfecta with ectopic mineralizations in dentin and cementum and a COL1A2 mutation has been reported in a Thai adult patient and his preschool daughter. PMID:29636545
This study showed that there is no association of collagen type-(2a) and intracranial aneurysms. PMID:29164999
High COL1A2 expression is associated with Gastric Cancer. PMID:28401451
The aim of this study is to determine whether EcoRI, Del38 and PvuII polymorphisms of COL1A2 are associated with the development of osteoporosis and osteopenia in post-menopausal Polish women. The analyzed COL1A2 polymorphisms seem to be related to osteoporosis development. The COL1A2 polymorphism may be a genetic risk factor related to the development of osteoporosis. PMID:28930368
COL1A2 gene mutation is associated with osteogenesis imperfecta. PMID:28810924
We evaluated the association of a 7-base pair (7-bp) indel polymorphism (rs3917) in the 3'UTR of COL1A2 with the risk of sudden cardiac death in a Chinese population. Our data provided initial evidence that rs3917 was highly relevant to SCD susceptibility, and this indel may become a potential marker for molecular diagnosis and genetic counseling of SCD. PMID:28738217
meta-analysis suggests COL1A2 rs42524 is a significant risk factor for Intracranial Aneurysm susceptibility. PMID:28671939
By acting probably as a posttranscriptional regulator with a different efficacy on COL2A1 and COL1A2 expression, miR-29b can contribute to the collagens imbalance associated with an abnormal chondrocyte phenotype. PMID:28612031
High methylation of COL1A2 is associated with head and neck cancer. PMID:27027429
a phenotypically distinctive group of OI is caused by a mono-allelic COL1 C-propeptide cleavage site mutations and biallelic BMP1 mutations. The subgroup is characterized by high bone mass and other unique skeletal changes, such as coarse trabeculae, pseudo-fractures, and metaphyseal constriction PMID:27264419
We suggest that the mutation p.G337C in the COL1A2 gene is pathogenic for osteogenesis imperfecta by affecting the protein structure and the function of collagen. PMID:28953610
Identify PDGFRbeta as a driver in activating Akt/mTORC1 nexus for high glucose-mediated expression of collagen I (alpha2) in proximal tubular epithelial cells, which contributes to tubulointerstitial fibrosis in diabetic nephropathy. PMID:28424212
Flightless-I (Drosophila) homolog (FLII) activates TGFbeta1-mediated expression of COL1A2 gene. PMID:25451260
In the individuals with a COL1A2 mutation, 80% (8/10) of those with a glycine substitution located C terminal of p.Gly211 exhibited DGI in both dentitions while no individual (0/5) with a mutation N-terminal of this point (p = 0.007) exhibited DGI in either dentition. PMID:28498836
COL1A2 expression was positively related to tumor size, depth of invasion, and to lower overall survival of gastric cancer patients. PMID:27894325
the present study demonstrated that the INS/DEL polymorphism in the 3'UTR of COL1A2 is able to interfere with the interaction between miRNA and mRNA. In addition, it is the first study, to the best of our knowledge, to indicate that the minor allele (Del) is associated with a reduced risk of developing osteoporosis. PMID:27665867
THBS2, but not COL1A2 and SPP1, may serve as an indicator of gastric cancer prognosis. PMID:27997896
In children aged 6-12, the presence of polymorphism in the COL1A2 gene was not associated with the severity of dental fluorosis. PMID:26564713
Study suggests that the COL1A2 rs42524 polymorphism is associated with the development of hypertensive intracerebral hemorrhage, particularly in conjunction with tobacco use and alcohol consumption. PMID:26910001
Two Rare Mutations in the COL1A2 Gene Associate With Low Bone Mineral Density and Fractures in Iceland. PMID:26235824
COL1A2 mutation is associated with atypical osteogenesis imperfect. PMID:26471105
analysis of COL1A2 (type I collagen) polymorphisms in the central-eastern Mediterranean Basin PMID:26065693
Severe osteogenesis imperfecta caused by double glycine substitutions near the amino-terminal triple helical region in COL1A2. PMID:25858481
These data indicate a novel functional role of AnxA2 in the negative post-transcriptional regulation of type I collagen synthesis in human fibroblasts. PMID:25290763
The novel c.946G>T/p.G316C mutation in COL1A2 gene is associated with with osteogenesis imperfecta type I. PMID:25608812
Extracellular matrix proteins expression profiling in chemoresistant variants of the A2780 ovarian cancer cell line. PMID:24804215
Data indicate that the IL-33/ST2 protein pathway may promote airway remodeling in asthma through activating HLF-1 fibroblast to over-express fibronectin 1 (FN1) and type 1 collagen (Col1). PMID:25200162
The COL1A2 gene is associated with intracranial aneurysms in a subset of the German population, but it is not responsible for the majority of aneurysms. PMID:23800505
these results indicate that under quiescent conditions Fli1 recruits HDAC1/p300 to the COL1A2 promoter and suppresses the expression of the COL1A2 gene by chromatin remodeling through histone deacetylation. PMID:24058639
this study highlights an essential role for Sin3B in IFN-c induced COL1A2 repression in smooth muscle cells. PMID:24709079
Missense or nonsense mutations in COL1A2 were identified in 2 unrelated patients with osteigenesis imperfecta. PMID:24140640
Expression of COL1A2 mRNA encoding key fibrotic extracellular matrix molecules was down-regulated by pre-miRNA-29b. PMID:24641356
Increased degradation of collagen type I was observed in the supernatant of KFs expressing siTIMP-1, but not siTIMP-2, with the suppression of cell viability and induction of apoptosis. PMID:24042342
Data suggest that common genetic variations in COL1A2 may influence chronic venous insufficiency risk, possibly through microRNA-382-mediated regulation. PMID:23849651
Family study of missense mutation of c.2279G>A, Gly760Glu in exon 37 of COL1A2 causing familial osteogenesis imperfecta type IV. PMID:23548243
We conclude that the COL1A2 rs42524 polymorphism could be a genetic risk factor for primary intracerebral hemorrhage among Han Chinese. PMID:23036172
A spontaneous novel mutation in COL1A2 (c.1171G>A; p.Gly391Ser) causing only dentin defects and a novel mutation in PAX9 (c.43T>A; p.Phe15Ile) causing hypodontia were identified and correlated with the phenotypic presentations in the family. PMID:23227268
these data have identified as novel pathway whereby SIRT1 maintains COL1A2 synthesis in SMCs by modulating RFX5 activity. PMID:23079621
found no evidence supporting the putative link of COL1A2 alleles with otosclerosis. PMID:22130917
Scleraxis thus appears to play a key role in the transcriptional regulation of type I collagen synthesis. PMID:22796342
The rs42524 polymorphism in COL1A2 is a risk allele for neovascular age-related macular degeneration (nAMD) in a Han Chinese population. PMID:22815632
Data suggest that the Collagen alpha2 (I) (COL1A2) gene may play a role in the tumorigenesis of head and neck squamous cell carcinoma (HNSCC) and may serve as an important biomarker. PMID:22674299
TGF-beta stimulation upregulated type I collagen expression via miR-196a downregulation in normal fibroblasts. Cotransfection of the 196a protector blocked the miR-196a inhibitor-mediated upregulation of a2(I) collagen. PMID:22379029
Matrix-mediated downregulation of type I alpha 2 collagen occurs zia an Sp1 transcription factor Pathway PMID:22131293
transcript levels of UCHL1, COL1A2, THBS1 and TNFRSF10D were inversely correlated with promoter methylation PMID:22028813
MRTF-A is an important regulator of collagen synthesis in lung fibroblasts and exhibits a dependence on both SRF and Sp1 function to enhance collagen expression PMID:22049076
The patient with COL1A2 Ala1119Thr mutation has mild osteogenesis imperfecta. PMID:21344539
This study identifies two novel mutations p.G1102A and p.Y1117C that cause osteogenesis imperfecta PMID:21530898
Genetic variation within the COL1A2 gene is associated with increased risk of hepatocellular carcinoma in a Chinese population. PMID:21665180
COL10A2 is upregulated in gastric adenocarcinoma and is associated with tumor progression. PMID:21443102