CNN1 Recombinant Monoclonal Antibody

Code: CSB-RA005655A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human, Mouse

Application: ELISA, WB, IHC

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Target Background

Product Details

Uniprot NO.

P51911

Target Names

CNN1

Alternative Names

Basic calponin antibody; Calponin 1 antibody; Calponin 1 basic smooth muscle antibody; Calponin H1 antibody; Calponin H1 smooth muscle antibody; Calponin-1 antibody; Calponin1 antibody; Calponins basic antibody; CNN 1 antibody; Cnn1 antibody; CNN1_HUMAN antibody; Epididymis secretory protein Li 14 antibody; HEL S 14 antibody; Sm Calp antibody; SMCC antibody; smooth muscle antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human CNN1

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2H1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: 293 whole cell lysate, Mouse lung tissue
All lanes: CNN1 antibody at 1.05μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 32 KDa
Observed band size: 34 KDa
IHC image of CSB-RA005655A0HU diluted at 1:105 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Description

Calponin-1 serves as a fundamental regulator of smooth muscle contraction, functioning as an actin-binding protein that modulates actomyosin interactions and cytoskeletal dynamics. As a definitive marker of smooth muscle differentiation, CNN1 plays essential roles in vascular biology, cardiac development, and tissue remodeling research. Its expression patterns provide valuable insights into smooth muscle phenotype switching, a process implicated in cardiovascular diseases and fibrotic conditions.

This recombinant monoclonal antibody, generated from clone 2H1, offers the reproducibility and consistency that demanding research protocols require. Because the antibody sequence is defined and production occurs in controlled recombinant systems, you can expect uniform performance across experiments and over time, eliminating the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG format, raised against a synthetic peptide derived from human CNN1, provides high specificity for your target.

Validation studies confirm reliable performance across multiple experimental platforms. In western blot applications, the antibody detects CNN1 at the expected 34 kDa molecular weight in both 293 whole cell lysate and mouse lung tissue, demonstrating cross-species reactivity between human and mouse samples. Recommended dilutions range from 1:500 to 1:5000, allowing you to optimize signal intensity for your specific sample types. For tissue localization studies, immunohistochemistry validation in paraffin-embedded human heart tissue shows clear staining using standard antigen retrieval protocols, with effective dilutions between 1:50 and 1:200.

Whether investigating smooth muscle differentiation, cardiovascular signal transduction pathways, or cytoskeletal regulation, this antibody provides a dependable tool for advancing your research objectives.

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Target Background

Function(From Uniprot)

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

Gene References into Functions

Studied maternal serum calponin 1 levels in the prediction of delivery within 7 days among pregnancies complicated with threatened preterm labor. Of 73 women included in the study, found the maternal serum calponin 1 level was significantly high in women who delivered within 7 days vs. those who delivered after 7 days. PMID:28068849
Loss of myoepithelial calponin was specifically associated with gain of the basal marker p63 in adjacent tumor cells. PMID:26343330
Loss of CNN1 expression is associated with Ovarian Cancer. PMID:26504022
CNN1 immunohistochemical staining allows ready recognition of intracytoplasmic inclusions, aiding diagnosis of infantile digital fibromatosis. PMID:24117680
epsilonPKC/ERK/mTOR pathway activation induced by the rescued expression of CNN1 contributed to the improvement of cardiac dysfunction and pathological changes observed in transgenic mice. PMID:24631115
Calponin knockdown inhibits autophosphorylation. PMID:24350264
Expression of transforming growth factor-beta (4.3 +/- 1.4 vs 2.6 +/- 2.3, p = 0.01) and calponin (3.7 +/- 0.7 vs 0.6 +/- 1.1, p = 0.05) was lower from infants with vs without a PDA. PMID:23731614
Calponin-1 expression in the uterine smooth muscle tissue of the body and the lower uterine segment of smooth muscle tissues was significantly different. PMID:21051832
Inhibition of calponin-1 expression can inhibit uterine smooth muscle cell migration and cause the morphological change and rearrangement of F-actin without affecting its proliferation and apoptosis in vitro. PMID:20584679
A single intronic CArG element is necessary but insufficient for proper CNN1 expression in vivo. CNN1 overexpression antagonizes arterial injury-induced neointimal formation. PMID:21817093
D2-40 immunohistochemistry reliably identifies the myoepithelial cells of breast in a variety of lesions in a pattern similar to that of calponin and p63. PMID:21326813
The point mutation S175T exhibited more resistance to actin rearrangement by cytochalasin D and PDBu than intact CNh1, suppressing cell motility induced by PDBu. PMID:20530411
expression in tumor vessels is a new and useful means to predict prognosis of hepatocellular carcinoma after hepatic resection PMID:11920541
results suggest that regulation of h1-calponin is involved in the regulation of differentiation of AR42J cells into insulin-producing cells PMID:12606518
the calponin homology domain of Vav1 associates with calmodulin and is prerequisite to T cell antigen receptor-induced calcium release in Jurkat T lymphocytes PMID:17550897
analysis of distinct sites of interaction that form the calponin: gelsolin complex and two calcium switches that control its activity PMID:17556051
expression of vascular CNN1 in the peripheral region of colon cancer tissues was significantly reduced in association with tumor progression, lymphatic invasion, vascular invasion and recurrence. PMID:17707120
Calponin and alpha-inhibin are the most useful positive markers for serous microcystic adenoma, and are negative in most entities in the differential diagnosis. PMID:19391217